Job ID = 14172329 SRX = SRX7434114 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 9451032 spots for SRR10759880/SRR10759880.sra Written 9451032 spots for SRR10759880/SRR10759880.sra fastq に変換しました。 bowtie でマッピング中... Your job 14172792 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:23 9451032 reads; of these: 9451032 (100.00%) were unpaired; of these: 1400215 (14.82%) aligned 0 times 6931159 (73.34%) aligned exactly 1 time 1119658 (11.85%) aligned >1 times 85.18% overall alignment rate Time searching: 00:02:24 Overall time: 00:02:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 5210702 / 8050817 = 0.6472 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 15:12:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7434114/SRX7434114.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7434114/SRX7434114.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7434114/SRX7434114.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7434114/SRX7434114.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 15:12:33: #1 read tag files... INFO @ Sat, 11 Dec 2021 15:12:33: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 15:12:39: 1000000 INFO @ Sat, 11 Dec 2021 15:12:46: 2000000 INFO @ Sat, 11 Dec 2021 15:12:52: #1 tag size is determined as 51 bps INFO @ Sat, 11 Dec 2021 15:12:52: #1 tag size = 51 INFO @ Sat, 11 Dec 2021 15:12:52: #1 total tags in treatment: 2840115 INFO @ Sat, 11 Dec 2021 15:12:52: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 15:12:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 15:12:52: #1 tags after filtering in treatment: 2840115 INFO @ Sat, 11 Dec 2021 15:12:52: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 15:12:52: #1 finished! INFO @ Sat, 11 Dec 2021 15:12:52: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 15:12:52: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 15:12:52: #2 number of paired peaks: 5662 INFO @ Sat, 11 Dec 2021 15:12:52: start model_add_line... INFO @ Sat, 11 Dec 2021 15:12:52: start X-correlation... INFO @ Sat, 11 Dec 2021 15:12:52: end of X-cor INFO @ Sat, 11 Dec 2021 15:12:52: #2 finished! INFO @ Sat, 11 Dec 2021 15:12:52: #2 predicted fragment length is 227 bps INFO @ Sat, 11 Dec 2021 15:12:52: #2 alternative fragment length(s) may be 227 bps INFO @ Sat, 11 Dec 2021 15:12:52: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX7434114/SRX7434114.05_model.r INFO @ Sat, 11 Dec 2021 15:12:52: #3 Call peaks... INFO @ Sat, 11 Dec 2021 15:12:52: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 15:12:59: #3 Call peaks for each chromosome... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 15:13:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7434114/SRX7434114.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7434114/SRX7434114.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7434114/SRX7434114.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7434114/SRX7434114.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 15:13:02: #1 read tag files... INFO @ Sat, 11 Dec 2021 15:13:02: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 15:13:02: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX7434114/SRX7434114.05_peaks.xls INFO @ Sat, 11 Dec 2021 15:13:02: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX7434114/SRX7434114.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 15:13:02: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX7434114/SRX7434114.05_summits.bed INFO @ Sat, 11 Dec 2021 15:13:02: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (7186 records, 4 fields): 8 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 15:13:08: 1000000 INFO @ Sat, 11 Dec 2021 15:13:13: 2000000 INFO @ Sat, 11 Dec 2021 15:13:18: #1 tag size is determined as 51 bps INFO @ Sat, 11 Dec 2021 15:13:18: #1 tag size = 51 INFO @ Sat, 11 Dec 2021 15:13:18: #1 total tags in treatment: 2840115 INFO @ Sat, 11 Dec 2021 15:13:18: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 15:13:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 15:13:18: #1 tags after filtering in treatment: 2840115 INFO @ Sat, 11 Dec 2021 15:13:18: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 15:13:18: #1 finished! INFO @ Sat, 11 Dec 2021 15:13:18: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 15:13:18: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 15:13:18: #2 number of paired peaks: 5662 INFO @ Sat, 11 Dec 2021 15:13:18: start model_add_line... INFO @ Sat, 11 Dec 2021 15:13:18: start X-correlation... INFO @ Sat, 11 Dec 2021 15:13:18: end of X-cor INFO @ Sat, 11 Dec 2021 15:13:18: #2 finished! INFO @ Sat, 11 Dec 2021 15:13:18: #2 predicted fragment length is 227 bps INFO @ Sat, 11 Dec 2021 15:13:18: #2 alternative fragment length(s) may be 227 bps INFO @ Sat, 11 Dec 2021 15:13:18: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX7434114/SRX7434114.10_model.r INFO @ Sat, 11 Dec 2021 15:13:18: #3 Call peaks... INFO @ Sat, 11 Dec 2021 15:13:18: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 15:13:26: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 15:13:29: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX7434114/SRX7434114.10_peaks.xls INFO @ Sat, 11 Dec 2021 15:13:29: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX7434114/SRX7434114.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 15:13:29: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX7434114/SRX7434114.10_summits.bed INFO @ Sat, 11 Dec 2021 15:13:29: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (5456 records, 4 fields): 6 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 15:13:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7434114/SRX7434114.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7434114/SRX7434114.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7434114/SRX7434114.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7434114/SRX7434114.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 15:13:32: #1 read tag files... INFO @ Sat, 11 Dec 2021 15:13:32: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 15:13:39: 1000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 15:13:45: 2000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 15:13:51: #1 tag size is determined as 51 bps INFO @ Sat, 11 Dec 2021 15:13:51: #1 tag size = 51 INFO @ Sat, 11 Dec 2021 15:13:51: #1 total tags in treatment: 2840115 INFO @ Sat, 11 Dec 2021 15:13:51: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 15:13:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 15:13:51: #1 tags after filtering in treatment: 2840115 INFO @ Sat, 11 Dec 2021 15:13:51: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 15:13:51: #1 finished! INFO @ Sat, 11 Dec 2021 15:13:51: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 15:13:51: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 15:13:51: #2 number of paired peaks: 5662 INFO @ Sat, 11 Dec 2021 15:13:51: start model_add_line... INFO @ Sat, 11 Dec 2021 15:13:51: start X-correlation... INFO @ Sat, 11 Dec 2021 15:13:51: end of X-cor INFO @ Sat, 11 Dec 2021 15:13:51: #2 finished! INFO @ Sat, 11 Dec 2021 15:13:51: #2 predicted fragment length is 227 bps INFO @ Sat, 11 Dec 2021 15:13:51: #2 alternative fragment length(s) may be 227 bps INFO @ Sat, 11 Dec 2021 15:13:51: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX7434114/SRX7434114.20_model.r INFO @ Sat, 11 Dec 2021 15:13:51: #3 Call peaks... INFO @ Sat, 11 Dec 2021 15:13:51: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 15:13:58: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 15:14:01: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX7434114/SRX7434114.20_peaks.xls INFO @ Sat, 11 Dec 2021 15:14:01: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX7434114/SRX7434114.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 15:14:02: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX7434114/SRX7434114.20_summits.bed INFO @ Sat, 11 Dec 2021 15:14:02: Done! pass1 - making usageList (13 chroms): 1 millis pass2 - checking and writing primary data (3601 records, 4 fields): 5 millis CompletedMACS2peakCalling