Job ID = 14172325 SRX = SRX7434111 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 27316832 spots for SRR10759877/SRR10759877.sra Written 27316832 spots for SRR10759877/SRR10759877.sra fastq に変換しました。 bowtie でマッピング中... Your job 14172822 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:28 27316832 reads; of these: 27316832 (100.00%) were unpaired; of these: 3011865 (11.03%) aligned 0 times 18184908 (66.57%) aligned exactly 1 time 6120059 (22.40%) aligned >1 times 88.97% overall alignment rate Time searching: 00:08:28 Overall time: 00:08:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 3160836 / 24304967 = 0.1300 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 15:23:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7434111/SRX7434111.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7434111/SRX7434111.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7434111/SRX7434111.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7434111/SRX7434111.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 15:23:01: #1 read tag files... INFO @ Sat, 11 Dec 2021 15:23:01: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 15:23:08: 1000000 INFO @ Sat, 11 Dec 2021 15:23:15: 2000000 INFO @ Sat, 11 Dec 2021 15:23:22: 3000000 INFO @ Sat, 11 Dec 2021 15:23:29: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 15:23:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7434111/SRX7434111.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7434111/SRX7434111.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7434111/SRX7434111.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7434111/SRX7434111.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 15:23:31: #1 read tag files... INFO @ Sat, 11 Dec 2021 15:23:31: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 15:23:36: 5000000 INFO @ Sat, 11 Dec 2021 15:23:38: 1000000 INFO @ Sat, 11 Dec 2021 15:23:43: 6000000 INFO @ Sat, 11 Dec 2021 15:23:46: 2000000 INFO @ Sat, 11 Dec 2021 15:23:51: 7000000 INFO @ Sat, 11 Dec 2021 15:23:54: 3000000 INFO @ Sat, 11 Dec 2021 15:23:59: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 15:24:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7434111/SRX7434111.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7434111/SRX7434111.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7434111/SRX7434111.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7434111/SRX7434111.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 15:24:01: #1 read tag files... INFO @ Sat, 11 Dec 2021 15:24:01: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 15:24:01: 4000000 INFO @ Sat, 11 Dec 2021 15:24:06: 9000000 INFO @ Sat, 11 Dec 2021 15:24:08: 1000000 INFO @ Sat, 11 Dec 2021 15:24:09: 5000000 INFO @ Sat, 11 Dec 2021 15:24:14: 10000000 INFO @ Sat, 11 Dec 2021 15:24:14: 2000000 INFO @ Sat, 11 Dec 2021 15:24:17: 6000000 INFO @ Sat, 11 Dec 2021 15:24:21: 3000000 INFO @ Sat, 11 Dec 2021 15:24:22: 11000000 INFO @ Sat, 11 Dec 2021 15:24:24: 7000000 INFO @ Sat, 11 Dec 2021 15:24:28: 4000000 INFO @ Sat, 11 Dec 2021 15:24:30: 12000000 INFO @ Sat, 11 Dec 2021 15:24:32: 8000000 INFO @ Sat, 11 Dec 2021 15:24:35: 5000000 INFO @ Sat, 11 Dec 2021 15:24:38: 13000000 INFO @ Sat, 11 Dec 2021 15:24:40: 9000000 INFO @ Sat, 11 Dec 2021 15:24:42: 6000000 INFO @ Sat, 11 Dec 2021 15:24:45: 14000000 INFO @ Sat, 11 Dec 2021 15:24:48: 10000000 INFO @ Sat, 11 Dec 2021 15:24:49: 7000000 INFO @ Sat, 11 Dec 2021 15:24:53: 15000000 INFO @ Sat, 11 Dec 2021 15:24:55: 11000000 INFO @ Sat, 11 Dec 2021 15:24:56: 8000000 INFO @ Sat, 11 Dec 2021 15:25:01: 16000000 INFO @ Sat, 11 Dec 2021 15:25:03: 9000000 INFO @ Sat, 11 Dec 2021 15:25:03: 12000000 INFO @ Sat, 11 Dec 2021 15:25:09: 17000000 INFO @ Sat, 11 Dec 2021 15:25:10: 10000000 INFO @ Sat, 11 Dec 2021 15:25:11: 13000000 INFO @ Sat, 11 Dec 2021 15:25:17: 11000000 INFO @ Sat, 11 Dec 2021 15:25:17: 18000000 INFO @ Sat, 11 Dec 2021 15:25:19: 14000000 INFO @ Sat, 11 Dec 2021 15:25:24: 12000000 INFO @ Sat, 11 Dec 2021 15:25:25: 19000000 INFO @ Sat, 11 Dec 2021 15:25:26: 15000000 INFO @ Sat, 11 Dec 2021 15:25:31: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 15:25:33: 20000000 INFO @ Sat, 11 Dec 2021 15:25:34: 16000000 INFO @ Sat, 11 Dec 2021 15:25:38: 14000000 INFO @ Sat, 11 Dec 2021 15:25:41: 21000000 INFO @ Sat, 11 Dec 2021 15:25:42: #1 tag size is determined as 51 bps INFO @ Sat, 11 Dec 2021 15:25:42: #1 tag size = 51 INFO @ Sat, 11 Dec 2021 15:25:42: #1 total tags in treatment: 21144131 INFO @ Sat, 11 Dec 2021 15:25:42: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 15:25:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 15:25:42: #1 tags after filtering in treatment: 21144131 INFO @ Sat, 11 Dec 2021 15:25:42: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 15:25:42: #1 finished! INFO @ Sat, 11 Dec 2021 15:25:42: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 15:25:42: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 15:25:42: 17000000 INFO @ Sat, 11 Dec 2021 15:25:44: #2 number of paired peaks: 85 WARNING @ Sat, 11 Dec 2021 15:25:44: Too few paired peaks (85) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 15:25:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7434111/SRX7434111.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7434111/SRX7434111.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7434111/SRX7434111.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7434111/SRX7434111.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 15:25:45: 15000000 INFO @ Sat, 11 Dec 2021 15:25:50: 18000000 INFO @ Sat, 11 Dec 2021 15:25:52: 16000000 INFO @ Sat, 11 Dec 2021 15:25:58: 19000000 INFO @ Sat, 11 Dec 2021 15:25:59: 17000000 INFO @ Sat, 11 Dec 2021 15:26:06: 18000000 INFO @ Sat, 11 Dec 2021 15:26:06: 20000000 INFO @ Sat, 11 Dec 2021 15:26:12: 19000000 INFO @ Sat, 11 Dec 2021 15:26:14: 21000000 INFO @ Sat, 11 Dec 2021 15:26:15: #1 tag size is determined as 51 bps INFO @ Sat, 11 Dec 2021 15:26:15: #1 tag size = 51 INFO @ Sat, 11 Dec 2021 15:26:15: #1 total tags in treatment: 21144131 INFO @ Sat, 11 Dec 2021 15:26:15: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 15:26:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 15:26:15: #1 tags after filtering in treatment: 21144131 INFO @ Sat, 11 Dec 2021 15:26:15: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 15:26:15: #1 finished! INFO @ Sat, 11 Dec 2021 15:26:15: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 15:26:15: #2 looking for paired plus/minus strand peaks... BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 15:26:17: #2 number of paired peaks: 85 WARNING @ Sat, 11 Dec 2021 15:26:17: Too few paired peaks (85) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 15:26:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7434111/SRX7434111.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7434111/SRX7434111.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7434111/SRX7434111.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7434111/SRX7434111.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 15:26:19: 20000000 INFO @ Sat, 11 Dec 2021 15:26:25: 21000000 INFO @ Sat, 11 Dec 2021 15:26:26: #1 tag size is determined as 51 bps INFO @ Sat, 11 Dec 2021 15:26:26: #1 tag size = 51 INFO @ Sat, 11 Dec 2021 15:26:26: #1 total tags in treatment: 21144131 INFO @ Sat, 11 Dec 2021 15:26:26: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 15:26:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 15:26:26: #1 tags after filtering in treatment: 21144131 INFO @ Sat, 11 Dec 2021 15:26:26: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 15:26:26: #1 finished! INFO @ Sat, 11 Dec 2021 15:26:26: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 15:26:26: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 15:26:27: #2 number of paired peaks: 85 WARNING @ Sat, 11 Dec 2021 15:26:27: Too few paired peaks (85) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 15:26:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7434111/SRX7434111.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7434111/SRX7434111.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7434111/SRX7434111.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7434111/SRX7434111.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling