Job ID = 14170614 SRX = SRX7320036 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 39133827 spots for SRR10640951/SRR10640951.sra Written 39133827 spots for SRR10640951/SRR10640951.sra fastq に変換しました。 bowtie でマッピング中... Your job 14171049 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:06 39133827 reads; of these: 39133827 (100.00%) were unpaired; of these: 2002019 (5.12%) aligned 0 times 27694143 (70.77%) aligned exactly 1 time 9437665 (24.12%) aligned >1 times 94.88% overall alignment rate Time searching: 00:11:06 Overall time: 00:11:06 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 6612858 / 37131808 = 0.1781 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 07:50:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7320036/SRX7320036.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7320036/SRX7320036.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7320036/SRX7320036.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7320036/SRX7320036.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 07:50:56: #1 read tag files... INFO @ Sat, 11 Dec 2021 07:50:56: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 07:51:01: 1000000 INFO @ Sat, 11 Dec 2021 07:51:07: 2000000 INFO @ Sat, 11 Dec 2021 07:51:13: 3000000 INFO @ Sat, 11 Dec 2021 07:51:18: 4000000 INFO @ Sat, 11 Dec 2021 07:51:24: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 07:51:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7320036/SRX7320036.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7320036/SRX7320036.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7320036/SRX7320036.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7320036/SRX7320036.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 07:51:26: #1 read tag files... INFO @ Sat, 11 Dec 2021 07:51:26: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 07:51:30: 6000000 INFO @ Sat, 11 Dec 2021 07:51:33: 1000000 INFO @ Sat, 11 Dec 2021 07:51:36: 7000000 INFO @ Sat, 11 Dec 2021 07:51:39: 2000000 INFO @ Sat, 11 Dec 2021 07:51:43: 8000000 INFO @ Sat, 11 Dec 2021 07:51:46: 3000000 INFO @ Sat, 11 Dec 2021 07:51:50: 9000000 INFO @ Sat, 11 Dec 2021 07:51:53: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 07:51:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7320036/SRX7320036.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7320036/SRX7320036.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7320036/SRX7320036.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7320036/SRX7320036.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 07:51:56: #1 read tag files... INFO @ Sat, 11 Dec 2021 07:51:56: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 07:51:56: 10000000 INFO @ Sat, 11 Dec 2021 07:51:59: 5000000 INFO @ Sat, 11 Dec 2021 07:52:03: 1000000 INFO @ Sat, 11 Dec 2021 07:52:03: 11000000 INFO @ Sat, 11 Dec 2021 07:52:06: 6000000 INFO @ Sat, 11 Dec 2021 07:52:09: 2000000 INFO @ Sat, 11 Dec 2021 07:52:10: 12000000 INFO @ Sat, 11 Dec 2021 07:52:13: 7000000 INFO @ Sat, 11 Dec 2021 07:52:16: 3000000 INFO @ Sat, 11 Dec 2021 07:52:16: 13000000 INFO @ Sat, 11 Dec 2021 07:52:19: 8000000 INFO @ Sat, 11 Dec 2021 07:52:22: 4000000 INFO @ Sat, 11 Dec 2021 07:52:23: 14000000 INFO @ Sat, 11 Dec 2021 07:52:26: 9000000 INFO @ Sat, 11 Dec 2021 07:52:29: 5000000 INFO @ Sat, 11 Dec 2021 07:52:30: 15000000 INFO @ Sat, 11 Dec 2021 07:52:33: 10000000 INFO @ Sat, 11 Dec 2021 07:52:36: 6000000 INFO @ Sat, 11 Dec 2021 07:52:36: 16000000 INFO @ Sat, 11 Dec 2021 07:52:40: 11000000 INFO @ Sat, 11 Dec 2021 07:52:42: 7000000 INFO @ Sat, 11 Dec 2021 07:52:43: 17000000 INFO @ Sat, 11 Dec 2021 07:52:46: 12000000 INFO @ Sat, 11 Dec 2021 07:52:49: 8000000 INFO @ Sat, 11 Dec 2021 07:52:50: 18000000 INFO @ Sat, 11 Dec 2021 07:52:53: 13000000 INFO @ Sat, 11 Dec 2021 07:52:55: 9000000 INFO @ Sat, 11 Dec 2021 07:52:56: 19000000 INFO @ Sat, 11 Dec 2021 07:53:00: 14000000 INFO @ Sat, 11 Dec 2021 07:53:02: 10000000 INFO @ Sat, 11 Dec 2021 07:53:03: 20000000 INFO @ Sat, 11 Dec 2021 07:53:06: 15000000 INFO @ Sat, 11 Dec 2021 07:53:09: 11000000 INFO @ Sat, 11 Dec 2021 07:53:09: 21000000 INFO @ Sat, 11 Dec 2021 07:53:13: 16000000 INFO @ Sat, 11 Dec 2021 07:53:15: 12000000 INFO @ Sat, 11 Dec 2021 07:53:16: 22000000 INFO @ Sat, 11 Dec 2021 07:53:20: 17000000 INFO @ Sat, 11 Dec 2021 07:53:22: 13000000 INFO @ Sat, 11 Dec 2021 07:53:23: 23000000 INFO @ Sat, 11 Dec 2021 07:53:26: 18000000 INFO @ Sat, 11 Dec 2021 07:53:28: 14000000 INFO @ Sat, 11 Dec 2021 07:53:29: 24000000 INFO @ Sat, 11 Dec 2021 07:53:33: 19000000 INFO @ Sat, 11 Dec 2021 07:53:35: 15000000 INFO @ Sat, 11 Dec 2021 07:53:36: 25000000 INFO @ Sat, 11 Dec 2021 07:53:40: 20000000 INFO @ Sat, 11 Dec 2021 07:53:42: 16000000 INFO @ Sat, 11 Dec 2021 07:53:43: 26000000 INFO @ Sat, 11 Dec 2021 07:53:46: 21000000 INFO @ Sat, 11 Dec 2021 07:53:48: 17000000 INFO @ Sat, 11 Dec 2021 07:53:50: 27000000 INFO @ Sat, 11 Dec 2021 07:53:53: 22000000 INFO @ Sat, 11 Dec 2021 07:53:55: 18000000 INFO @ Sat, 11 Dec 2021 07:53:56: 28000000 INFO @ Sat, 11 Dec 2021 07:54:00: 23000000 INFO @ Sat, 11 Dec 2021 07:54:01: 19000000 INFO @ Sat, 11 Dec 2021 07:54:03: 29000000 INFO @ Sat, 11 Dec 2021 07:54:06: 24000000 INFO @ Sat, 11 Dec 2021 07:54:08: 20000000 INFO @ Sat, 11 Dec 2021 07:54:10: 30000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 07:54:13: #1 tag size is determined as 49 bps INFO @ Sat, 11 Dec 2021 07:54:13: #1 tag size = 49 INFO @ Sat, 11 Dec 2021 07:54:13: #1 total tags in treatment: 30518950 INFO @ Sat, 11 Dec 2021 07:54:13: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 07:54:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 07:54:13: 25000000 INFO @ Sat, 11 Dec 2021 07:54:14: #1 tags after filtering in treatment: 30518950 INFO @ Sat, 11 Dec 2021 07:54:14: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 07:54:14: #1 finished! INFO @ Sat, 11 Dec 2021 07:54:14: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 07:54:14: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 07:54:15: 21000000 INFO @ Sat, 11 Dec 2021 07:54:15: #2 number of paired peaks: 61 WARNING @ Sat, 11 Dec 2021 07:54:15: Too few paired peaks (61) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 07:54:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7320036/SRX7320036.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7320036/SRX7320036.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7320036/SRX7320036.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7320036/SRX7320036.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 07:54:20: 26000000 INFO @ Sat, 11 Dec 2021 07:54:21: 22000000 INFO @ Sat, 11 Dec 2021 07:54:27: 27000000 INFO @ Sat, 11 Dec 2021 07:54:28: 23000000 INFO @ Sat, 11 Dec 2021 07:54:33: 28000000 INFO @ Sat, 11 Dec 2021 07:54:34: 24000000 INFO @ Sat, 11 Dec 2021 07:54:40: 29000000 INFO @ Sat, 11 Dec 2021 07:54:41: 25000000 INFO @ Sat, 11 Dec 2021 07:54:47: 30000000 INFO @ Sat, 11 Dec 2021 07:54:48: 26000000 INFO @ Sat, 11 Dec 2021 07:54:50: #1 tag size is determined as 49 bps INFO @ Sat, 11 Dec 2021 07:54:50: #1 tag size = 49 INFO @ Sat, 11 Dec 2021 07:54:50: #1 total tags in treatment: 30518950 INFO @ Sat, 11 Dec 2021 07:54:50: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 07:54:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 07:54:51: #1 tags after filtering in treatment: 30518950 INFO @ Sat, 11 Dec 2021 07:54:51: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 07:54:51: #1 finished! INFO @ Sat, 11 Dec 2021 07:54:51: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 07:54:51: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 07:54:52: #2 number of paired peaks: 61 WARNING @ Sat, 11 Dec 2021 07:54:52: Too few paired peaks (61) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 07:54:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7320036/SRX7320036.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7320036/SRX7320036.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7320036/SRX7320036.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7320036/SRX7320036.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 07:54:54: 27000000 INFO @ Sat, 11 Dec 2021 07:55:00: 28000000 INFO @ Sat, 11 Dec 2021 07:55:06: 29000000 INFO @ Sat, 11 Dec 2021 07:55:12: 30000000 INFO @ Sat, 11 Dec 2021 07:55:16: #1 tag size is determined as 49 bps INFO @ Sat, 11 Dec 2021 07:55:16: #1 tag size = 49 INFO @ Sat, 11 Dec 2021 07:55:16: #1 total tags in treatment: 30518950 INFO @ Sat, 11 Dec 2021 07:55:16: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 07:55:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 07:55:16: #1 tags after filtering in treatment: 30518950 INFO @ Sat, 11 Dec 2021 07:55:16: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 07:55:16: #1 finished! INFO @ Sat, 11 Dec 2021 07:55:16: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 07:55:16: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 07:55:18: #2 number of paired peaks: 61 WARNING @ Sat, 11 Dec 2021 07:55:18: Too few paired peaks (61) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 07:55:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7320036/SRX7320036.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7320036/SRX7320036.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7320036/SRX7320036.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7320036/SRX7320036.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。