Job ID = 14170611 SRX = SRX7320035 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 46935461 spots for SRR10640949/SRR10640949.sra Written 46935461 spots for SRR10640949/SRR10640949.sra Read 34047434 spots for SRR10640950/SRR10640950.sra Written 34047434 spots for SRR10640950/SRR10640950.sra fastq に変換しました。 bowtie でマッピング中... Your job 14171097 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:24:16 80982895 reads; of these: 80982895 (100.00%) were unpaired; of these: 3005034 (3.71%) aligned 0 times 64452149 (79.59%) aligned exactly 1 time 13525712 (16.70%) aligned >1 times 96.29% overall alignment rate Time searching: 00:24:16 Overall time: 00:24:16 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 32 files... [bam_rmdupse_core] 20963212 / 77977861 = 0.2688 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 08:17:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7320035/SRX7320035.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7320035/SRX7320035.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7320035/SRX7320035.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7320035/SRX7320035.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 08:17:11: #1 read tag files... INFO @ Sat, 11 Dec 2021 08:17:11: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 08:17:16: 1000000 INFO @ Sat, 11 Dec 2021 08:17:21: 2000000 INFO @ Sat, 11 Dec 2021 08:17:25: 3000000 INFO @ Sat, 11 Dec 2021 08:17:30: 4000000 INFO @ Sat, 11 Dec 2021 08:17:35: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 08:17:39: 6000000 INFO @ Sat, 11 Dec 2021 08:17:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7320035/SRX7320035.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7320035/SRX7320035.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7320035/SRX7320035.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7320035/SRX7320035.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 08:17:41: #1 read tag files... INFO @ Sat, 11 Dec 2021 08:17:41: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 08:17:44: 7000000 INFO @ Sat, 11 Dec 2021 08:17:46: 1000000 INFO @ Sat, 11 Dec 2021 08:17:49: 8000000 INFO @ Sat, 11 Dec 2021 08:17:51: 2000000 INFO @ Sat, 11 Dec 2021 08:17:54: 9000000 INFO @ Sat, 11 Dec 2021 08:17:56: 3000000 INFO @ Sat, 11 Dec 2021 08:17:59: 10000000 INFO @ Sat, 11 Dec 2021 08:18:01: 4000000 INFO @ Sat, 11 Dec 2021 08:18:04: 11000000 INFO @ Sat, 11 Dec 2021 08:18:06: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 08:18:09: 12000000 INFO @ Sat, 11 Dec 2021 08:18:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7320035/SRX7320035.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7320035/SRX7320035.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7320035/SRX7320035.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7320035/SRX7320035.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 08:18:11: #1 read tag files... INFO @ Sat, 11 Dec 2021 08:18:11: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 08:18:11: 6000000 INFO @ Sat, 11 Dec 2021 08:18:14: 13000000 INFO @ Sat, 11 Dec 2021 08:18:16: 1000000 INFO @ Sat, 11 Dec 2021 08:18:16: 7000000 INFO @ Sat, 11 Dec 2021 08:18:19: 14000000 INFO @ Sat, 11 Dec 2021 08:18:21: 2000000 INFO @ Sat, 11 Dec 2021 08:18:21: 8000000 INFO @ Sat, 11 Dec 2021 08:18:25: 15000000 INFO @ Sat, 11 Dec 2021 08:18:26: 3000000 INFO @ Sat, 11 Dec 2021 08:18:27: 9000000 INFO @ Sat, 11 Dec 2021 08:18:30: 16000000 INFO @ Sat, 11 Dec 2021 08:18:30: 4000000 INFO @ Sat, 11 Dec 2021 08:18:32: 10000000 INFO @ Sat, 11 Dec 2021 08:18:35: 17000000 INFO @ Sat, 11 Dec 2021 08:18:35: 5000000 INFO @ Sat, 11 Dec 2021 08:18:37: 11000000 INFO @ Sat, 11 Dec 2021 08:18:40: 18000000 INFO @ Sat, 11 Dec 2021 08:18:40: 6000000 INFO @ Sat, 11 Dec 2021 08:18:42: 12000000 INFO @ Sat, 11 Dec 2021 08:18:45: 7000000 INFO @ Sat, 11 Dec 2021 08:18:45: 19000000 INFO @ Sat, 11 Dec 2021 08:18:47: 13000000 INFO @ Sat, 11 Dec 2021 08:18:50: 8000000 INFO @ Sat, 11 Dec 2021 08:18:50: 20000000 INFO @ Sat, 11 Dec 2021 08:18:52: 14000000 INFO @ Sat, 11 Dec 2021 08:18:54: 9000000 INFO @ Sat, 11 Dec 2021 08:18:55: 21000000 INFO @ Sat, 11 Dec 2021 08:18:57: 15000000 INFO @ Sat, 11 Dec 2021 08:18:59: 10000000 INFO @ Sat, 11 Dec 2021 08:19:00: 22000000 INFO @ Sat, 11 Dec 2021 08:19:02: 16000000 INFO @ Sat, 11 Dec 2021 08:19:04: 11000000 INFO @ Sat, 11 Dec 2021 08:19:05: 23000000 INFO @ Sat, 11 Dec 2021 08:19:07: 17000000 INFO @ Sat, 11 Dec 2021 08:19:09: 12000000 INFO @ Sat, 11 Dec 2021 08:19:11: 24000000 INFO @ Sat, 11 Dec 2021 08:19:13: 18000000 INFO @ Sat, 11 Dec 2021 08:19:14: 13000000 INFO @ Sat, 11 Dec 2021 08:19:16: 25000000 INFO @ Sat, 11 Dec 2021 08:19:18: 19000000 INFO @ Sat, 11 Dec 2021 08:19:18: 14000000 INFO @ Sat, 11 Dec 2021 08:19:21: 26000000 INFO @ Sat, 11 Dec 2021 08:19:23: 15000000 INFO @ Sat, 11 Dec 2021 08:19:24: 20000000 INFO @ Sat, 11 Dec 2021 08:19:26: 27000000 INFO @ Sat, 11 Dec 2021 08:19:28: 16000000 INFO @ Sat, 11 Dec 2021 08:19:29: 21000000 INFO @ Sat, 11 Dec 2021 08:19:31: 28000000 INFO @ Sat, 11 Dec 2021 08:19:33: 17000000 INFO @ Sat, 11 Dec 2021 08:19:35: 22000000 INFO @ Sat, 11 Dec 2021 08:19:36: 29000000 INFO @ Sat, 11 Dec 2021 08:19:38: 18000000 INFO @ Sat, 11 Dec 2021 08:19:40: 23000000 INFO @ Sat, 11 Dec 2021 08:19:41: 30000000 INFO @ Sat, 11 Dec 2021 08:19:42: 19000000 INFO @ Sat, 11 Dec 2021 08:19:45: 24000000 INFO @ Sat, 11 Dec 2021 08:19:46: 31000000 INFO @ Sat, 11 Dec 2021 08:19:47: 20000000 INFO @ Sat, 11 Dec 2021 08:19:51: 25000000 INFO @ Sat, 11 Dec 2021 08:19:51: 32000000 INFO @ Sat, 11 Dec 2021 08:19:53: 21000000 INFO @ Sat, 11 Dec 2021 08:19:56: 26000000 INFO @ Sat, 11 Dec 2021 08:19:56: 33000000 INFO @ Sat, 11 Dec 2021 08:19:58: 22000000 INFO @ Sat, 11 Dec 2021 08:20:01: 34000000 INFO @ Sat, 11 Dec 2021 08:20:02: 27000000 INFO @ Sat, 11 Dec 2021 08:20:03: 23000000 INFO @ Sat, 11 Dec 2021 08:20:06: 35000000 INFO @ Sat, 11 Dec 2021 08:20:07: 28000000 INFO @ Sat, 11 Dec 2021 08:20:07: 24000000 INFO @ Sat, 11 Dec 2021 08:20:12: 36000000 INFO @ Sat, 11 Dec 2021 08:20:12: 25000000 INFO @ Sat, 11 Dec 2021 08:20:12: 29000000 INFO @ Sat, 11 Dec 2021 08:20:16: 37000000 INFO @ Sat, 11 Dec 2021 08:20:17: 26000000 INFO @ Sat, 11 Dec 2021 08:20:19: 30000000 INFO @ Sat, 11 Dec 2021 08:20:21: 38000000 INFO @ Sat, 11 Dec 2021 08:20:22: 27000000 INFO @ Sat, 11 Dec 2021 08:20:24: 31000000 INFO @ Sat, 11 Dec 2021 08:20:26: 39000000 INFO @ Sat, 11 Dec 2021 08:20:27: 28000000 INFO @ Sat, 11 Dec 2021 08:20:29: 32000000 INFO @ Sat, 11 Dec 2021 08:20:31: 40000000 INFO @ Sat, 11 Dec 2021 08:20:32: 29000000 INFO @ Sat, 11 Dec 2021 08:20:34: 33000000 INFO @ Sat, 11 Dec 2021 08:20:36: 41000000 INFO @ Sat, 11 Dec 2021 08:20:37: 30000000 INFO @ Sat, 11 Dec 2021 08:20:39: 34000000 INFO @ Sat, 11 Dec 2021 08:20:41: 31000000 INFO @ Sat, 11 Dec 2021 08:20:41: 42000000 INFO @ Sat, 11 Dec 2021 08:20:44: 35000000 INFO @ Sat, 11 Dec 2021 08:20:46: 32000000 INFO @ Sat, 11 Dec 2021 08:20:46: 43000000 INFO @ Sat, 11 Dec 2021 08:20:49: 36000000 INFO @ Sat, 11 Dec 2021 08:20:51: 33000000 INFO @ Sat, 11 Dec 2021 08:20:52: 44000000 INFO @ Sat, 11 Dec 2021 08:20:55: 37000000 INFO @ Sat, 11 Dec 2021 08:20:56: 34000000 INFO @ Sat, 11 Dec 2021 08:20:57: 45000000 INFO @ Sat, 11 Dec 2021 08:21:00: 38000000 INFO @ Sat, 11 Dec 2021 08:21:00: 35000000 INFO @ Sat, 11 Dec 2021 08:21:02: 46000000 INFO @ Sat, 11 Dec 2021 08:21:05: 39000000 INFO @ Sat, 11 Dec 2021 08:21:05: 36000000 INFO @ Sat, 11 Dec 2021 08:21:07: 47000000 INFO @ Sat, 11 Dec 2021 08:21:10: 37000000 INFO @ Sat, 11 Dec 2021 08:21:10: 40000000 INFO @ Sat, 11 Dec 2021 08:21:12: 48000000 INFO @ Sat, 11 Dec 2021 08:21:15: 38000000 INFO @ Sat, 11 Dec 2021 08:21:15: 41000000 INFO @ Sat, 11 Dec 2021 08:21:18: 49000000 INFO @ Sat, 11 Dec 2021 08:21:19: 39000000 INFO @ Sat, 11 Dec 2021 08:21:20: 42000000 INFO @ Sat, 11 Dec 2021 08:21:23: 50000000 INFO @ Sat, 11 Dec 2021 08:21:24: 40000000 INFO @ Sat, 11 Dec 2021 08:21:25: 43000000 INFO @ Sat, 11 Dec 2021 08:21:28: 51000000 INFO @ Sat, 11 Dec 2021 08:21:29: 41000000 INFO @ Sat, 11 Dec 2021 08:21:30: 44000000 INFO @ Sat, 11 Dec 2021 08:21:33: 52000000 INFO @ Sat, 11 Dec 2021 08:21:33: 42000000 INFO @ Sat, 11 Dec 2021 08:21:35: 45000000 INFO @ Sat, 11 Dec 2021 08:21:38: 53000000 INFO @ Sat, 11 Dec 2021 08:21:38: 43000000 INFO @ Sat, 11 Dec 2021 08:21:40: 46000000 INFO @ Sat, 11 Dec 2021 08:21:43: 44000000 INFO @ Sat, 11 Dec 2021 08:21:43: 54000000 INFO @ Sat, 11 Dec 2021 08:21:46: 47000000 INFO @ Sat, 11 Dec 2021 08:21:48: 45000000 INFO @ Sat, 11 Dec 2021 08:21:48: 55000000 INFO @ Sat, 11 Dec 2021 08:21:51: 48000000 INFO @ Sat, 11 Dec 2021 08:21:53: 46000000 INFO @ Sat, 11 Dec 2021 08:21:53: 56000000 INFO @ Sat, 11 Dec 2021 08:21:56: 49000000 INFO @ Sat, 11 Dec 2021 08:21:57: 47000000 INFO @ Sat, 11 Dec 2021 08:21:58: 57000000 INFO @ Sat, 11 Dec 2021 08:21:59: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 08:21:59: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 08:21:59: #1 total tags in treatment: 57014649 INFO @ Sat, 11 Dec 2021 08:21:59: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 08:21:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 08:22:00: #1 tags after filtering in treatment: 57014649 INFO @ Sat, 11 Dec 2021 08:22:00: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 08:22:00: #1 finished! INFO @ Sat, 11 Dec 2021 08:22:00: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 08:22:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 08:22:01: 50000000 INFO @ Sat, 11 Dec 2021 08:22:02: 48000000 INFO @ Sat, 11 Dec 2021 08:22:04: #2 number of paired peaks: 0 WARNING @ Sat, 11 Dec 2021 08:22:04: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 08:22:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7320035/SRX7320035.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7320035/SRX7320035.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7320035/SRX7320035.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7320035/SRX7320035.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 08:22:06: 51000000 INFO @ Sat, 11 Dec 2021 08:22:07: 49000000 INFO @ Sat, 11 Dec 2021 08:22:10: 52000000 INFO @ Sat, 11 Dec 2021 08:22:12: 50000000 INFO @ Sat, 11 Dec 2021 08:22:15: 53000000 INFO @ Sat, 11 Dec 2021 08:22:17: 51000000 INFO @ Sat, 11 Dec 2021 08:22:20: 54000000 INFO @ Sat, 11 Dec 2021 08:22:21: 52000000 INFO @ Sat, 11 Dec 2021 08:22:24: 55000000 INFO @ Sat, 11 Dec 2021 08:22:26: 53000000 INFO @ Sat, 11 Dec 2021 08:22:29: 56000000 INFO @ Sat, 11 Dec 2021 08:22:31: 54000000 INFO @ Sat, 11 Dec 2021 08:22:34: 57000000 INFO @ Sat, 11 Dec 2021 08:22:34: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 08:22:34: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 08:22:34: #1 total tags in treatment: 57014649 INFO @ Sat, 11 Dec 2021 08:22:34: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 08:22:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 08:22:35: 55000000 INFO @ Sat, 11 Dec 2021 08:22:36: #1 tags after filtering in treatment: 57014649 INFO @ Sat, 11 Dec 2021 08:22:36: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 08:22:36: #1 finished! INFO @ Sat, 11 Dec 2021 08:22:36: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 08:22:36: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 08:22:39: #2 number of paired peaks: 0 WARNING @ Sat, 11 Dec 2021 08:22:39: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 08:22:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7320035/SRX7320035.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7320035/SRX7320035.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7320035/SRX7320035.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7320035/SRX7320035.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 08:22:40: 56000000 INFO @ Sat, 11 Dec 2021 08:22:45: 57000000 INFO @ Sat, 11 Dec 2021 08:22:45: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 08:22:45: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 08:22:45: #1 total tags in treatment: 57014649 INFO @ Sat, 11 Dec 2021 08:22:45: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 08:22:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 08:22:46: #1 tags after filtering in treatment: 57014649 INFO @ Sat, 11 Dec 2021 08:22:46: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 08:22:46: #1 finished! INFO @ Sat, 11 Dec 2021 08:22:46: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 08:22:46: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 08:22:50: #2 number of paired peaks: 0 WARNING @ Sat, 11 Dec 2021 08:22:50: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 08:22:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7320035/SRX7320035.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7320035/SRX7320035.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7320035/SRX7320035.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7320035/SRX7320035.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。