Job ID = 14170610 SRX = SRX7320034 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 41942416 spots for SRR10640948/SRR10640948.sra Written 41942416 spots for SRR10640948/SRR10640948.sra fastq に変換しました。 bowtie でマッピング中... Your job 14171055 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:20 41942416 reads; of these: 41942416 (100.00%) were unpaired; of these: 1567879 (3.74%) aligned 0 times 30250033 (72.12%) aligned exactly 1 time 10124504 (24.14%) aligned >1 times 96.26% overall alignment rate Time searching: 00:12:20 Overall time: 00:12:20 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 7443248 / 40374537 = 0.1844 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 07:52:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7320034/SRX7320034.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7320034/SRX7320034.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7320034/SRX7320034.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7320034/SRX7320034.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 07:52:15: #1 read tag files... INFO @ Sat, 11 Dec 2021 07:52:15: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 07:52:21: 1000000 INFO @ Sat, 11 Dec 2021 07:52:26: 2000000 INFO @ Sat, 11 Dec 2021 07:52:31: 3000000 INFO @ Sat, 11 Dec 2021 07:52:36: 4000000 INFO @ Sat, 11 Dec 2021 07:52:41: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 07:52:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7320034/SRX7320034.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7320034/SRX7320034.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7320034/SRX7320034.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7320034/SRX7320034.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 07:52:45: #1 read tag files... INFO @ Sat, 11 Dec 2021 07:52:45: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 07:52:47: 6000000 INFO @ Sat, 11 Dec 2021 07:52:51: 1000000 INFO @ Sat, 11 Dec 2021 07:52:52: 7000000 INFO @ Sat, 11 Dec 2021 07:52:57: 2000000 INFO @ Sat, 11 Dec 2021 07:52:58: 8000000 INFO @ Sat, 11 Dec 2021 07:53:02: 3000000 INFO @ Sat, 11 Dec 2021 07:53:04: 9000000 INFO @ Sat, 11 Dec 2021 07:53:08: 4000000 INFO @ Sat, 11 Dec 2021 07:53:09: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 07:53:14: 5000000 INFO @ Sat, 11 Dec 2021 07:53:15: 11000000 INFO @ Sat, 11 Dec 2021 07:53:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7320034/SRX7320034.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7320034/SRX7320034.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7320034/SRX7320034.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7320034/SRX7320034.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 07:53:15: #1 read tag files... INFO @ Sat, 11 Dec 2021 07:53:15: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 07:53:19: 6000000 INFO @ Sat, 11 Dec 2021 07:53:21: 12000000 INFO @ Sat, 11 Dec 2021 07:53:21: 1000000 INFO @ Sat, 11 Dec 2021 07:53:25: 7000000 INFO @ Sat, 11 Dec 2021 07:53:26: 13000000 INFO @ Sat, 11 Dec 2021 07:53:27: 2000000 INFO @ Sat, 11 Dec 2021 07:53:30: 8000000 INFO @ Sat, 11 Dec 2021 07:53:32: 14000000 INFO @ Sat, 11 Dec 2021 07:53:33: 3000000 INFO @ Sat, 11 Dec 2021 07:53:36: 9000000 INFO @ Sat, 11 Dec 2021 07:53:38: 15000000 INFO @ Sat, 11 Dec 2021 07:53:39: 4000000 INFO @ Sat, 11 Dec 2021 07:53:42: 10000000 INFO @ Sat, 11 Dec 2021 07:53:43: 16000000 INFO @ Sat, 11 Dec 2021 07:53:45: 5000000 INFO @ Sat, 11 Dec 2021 07:53:47: 11000000 INFO @ Sat, 11 Dec 2021 07:53:49: 17000000 INFO @ Sat, 11 Dec 2021 07:53:51: 6000000 INFO @ Sat, 11 Dec 2021 07:53:53: 12000000 INFO @ Sat, 11 Dec 2021 07:53:55: 18000000 INFO @ Sat, 11 Dec 2021 07:53:56: 7000000 INFO @ Sat, 11 Dec 2021 07:53:59: 13000000 INFO @ Sat, 11 Dec 2021 07:54:00: 19000000 INFO @ Sat, 11 Dec 2021 07:54:03: 8000000 INFO @ Sat, 11 Dec 2021 07:54:05: 14000000 INFO @ Sat, 11 Dec 2021 07:54:06: 20000000 INFO @ Sat, 11 Dec 2021 07:54:09: 9000000 INFO @ Sat, 11 Dec 2021 07:54:10: 15000000 INFO @ Sat, 11 Dec 2021 07:54:12: 21000000 INFO @ Sat, 11 Dec 2021 07:54:15: 10000000 INFO @ Sat, 11 Dec 2021 07:54:16: 16000000 INFO @ Sat, 11 Dec 2021 07:54:18: 22000000 INFO @ Sat, 11 Dec 2021 07:54:20: 11000000 INFO @ Sat, 11 Dec 2021 07:54:22: 17000000 INFO @ Sat, 11 Dec 2021 07:54:23: 23000000 INFO @ Sat, 11 Dec 2021 07:54:26: 12000000 INFO @ Sat, 11 Dec 2021 07:54:27: 18000000 INFO @ Sat, 11 Dec 2021 07:54:29: 24000000 INFO @ Sat, 11 Dec 2021 07:54:32: 13000000 INFO @ Sat, 11 Dec 2021 07:54:33: 19000000 INFO @ Sat, 11 Dec 2021 07:54:35: 25000000 INFO @ Sat, 11 Dec 2021 07:54:38: 14000000 INFO @ Sat, 11 Dec 2021 07:54:38: 20000000 INFO @ Sat, 11 Dec 2021 07:54:40: 26000000 INFO @ Sat, 11 Dec 2021 07:54:44: 15000000 INFO @ Sat, 11 Dec 2021 07:54:44: 21000000 INFO @ Sat, 11 Dec 2021 07:54:46: 27000000 INFO @ Sat, 11 Dec 2021 07:54:49: 22000000 INFO @ Sat, 11 Dec 2021 07:54:50: 16000000 INFO @ Sat, 11 Dec 2021 07:54:52: 28000000 INFO @ Sat, 11 Dec 2021 07:54:55: 23000000 INFO @ Sat, 11 Dec 2021 07:54:55: 17000000 INFO @ Sat, 11 Dec 2021 07:54:57: 29000000 INFO @ Sat, 11 Dec 2021 07:55:01: 24000000 INFO @ Sat, 11 Dec 2021 07:55:01: 18000000 INFO @ Sat, 11 Dec 2021 07:55:03: 30000000 INFO @ Sat, 11 Dec 2021 07:55:06: 25000000 INFO @ Sat, 11 Dec 2021 07:55:07: 19000000 INFO @ Sat, 11 Dec 2021 07:55:08: 31000000 INFO @ Sat, 11 Dec 2021 07:55:12: 26000000 INFO @ Sat, 11 Dec 2021 07:55:13: 20000000 INFO @ Sat, 11 Dec 2021 07:55:14: 32000000 INFO @ Sat, 11 Dec 2021 07:55:18: 27000000 INFO @ Sat, 11 Dec 2021 07:55:19: 21000000 INFO @ Sat, 11 Dec 2021 07:55:20: #1 tag size is determined as 49 bps INFO @ Sat, 11 Dec 2021 07:55:20: #1 tag size = 49 INFO @ Sat, 11 Dec 2021 07:55:20: #1 total tags in treatment: 32931289 INFO @ Sat, 11 Dec 2021 07:55:20: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 07:55:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 07:55:20: #1 tags after filtering in treatment: 32931289 INFO @ Sat, 11 Dec 2021 07:55:20: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 07:55:20: #1 finished! INFO @ Sat, 11 Dec 2021 07:55:20: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 07:55:20: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 07:55:23: #2 number of paired peaks: 61 WARNING @ Sat, 11 Dec 2021 07:55:23: Too few paired peaks (61) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 07:55:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7320034/SRX7320034.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7320034/SRX7320034.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7320034/SRX7320034.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7320034/SRX7320034.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 07:55:24: 28000000 INFO @ Sat, 11 Dec 2021 07:55:25: 22000000 INFO @ Sat, 11 Dec 2021 07:55:29: 29000000 INFO @ Sat, 11 Dec 2021 07:55:31: 23000000 INFO @ Sat, 11 Dec 2021 07:55:35: 30000000 INFO @ Sat, 11 Dec 2021 07:55:36: 24000000 INFO @ Sat, 11 Dec 2021 07:55:40: 31000000 INFO @ Sat, 11 Dec 2021 07:55:42: 25000000 INFO @ Sat, 11 Dec 2021 07:55:46: 32000000 INFO @ Sat, 11 Dec 2021 07:55:48: 26000000 INFO @ Sat, 11 Dec 2021 07:55:51: #1 tag size is determined as 49 bps INFO @ Sat, 11 Dec 2021 07:55:51: #1 tag size = 49 INFO @ Sat, 11 Dec 2021 07:55:51: #1 total tags in treatment: 32931289 INFO @ Sat, 11 Dec 2021 07:55:51: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 07:55:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 07:55:52: #1 tags after filtering in treatment: 32931289 INFO @ Sat, 11 Dec 2021 07:55:52: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 07:55:52: #1 finished! INFO @ Sat, 11 Dec 2021 07:55:52: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 07:55:52: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 07:55:53: 27000000 INFO @ Sat, 11 Dec 2021 07:55:54: #2 number of paired peaks: 61 WARNING @ Sat, 11 Dec 2021 07:55:54: Too few paired peaks (61) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 07:55:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7320034/SRX7320034.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7320034/SRX7320034.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7320034/SRX7320034.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7320034/SRX7320034.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 07:55:59: 28000000 INFO @ Sat, 11 Dec 2021 07:56:04: 29000000 INFO @ Sat, 11 Dec 2021 07:56:10: 30000000 INFO @ Sat, 11 Dec 2021 07:56:15: 31000000 INFO @ Sat, 11 Dec 2021 07:56:21: 32000000 INFO @ Sat, 11 Dec 2021 07:56:27: #1 tag size is determined as 49 bps INFO @ Sat, 11 Dec 2021 07:56:27: #1 tag size = 49 INFO @ Sat, 11 Dec 2021 07:56:27: #1 total tags in treatment: 32931289 INFO @ Sat, 11 Dec 2021 07:56:27: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 07:56:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 07:56:27: #1 tags after filtering in treatment: 32931289 INFO @ Sat, 11 Dec 2021 07:56:27: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 07:56:27: #1 finished! INFO @ Sat, 11 Dec 2021 07:56:27: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 07:56:27: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 07:56:29: #2 number of paired peaks: 61 WARNING @ Sat, 11 Dec 2021 07:56:29: Too few paired peaks (61) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 07:56:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7320034/SRX7320034.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7320034/SRX7320034.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7320034/SRX7320034.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7320034/SRX7320034.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。