Job ID = 14170607 SRX = SRX7320031 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 38202080 spots for SRR10640943/SRR10640943.sra Written 38202080 spots for SRR10640943/SRR10640943.sra Read 53107372 spots for SRR10640944/SRR10640944.sra Written 53107372 spots for SRR10640944/SRR10640944.sra fastq に変換しました。 bowtie でマッピング中... Your job 14171093 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:25:19 91309452 reads; of these: 91309452 (100.00%) were unpaired; of these: 2749445 (3.01%) aligned 0 times 74305131 (81.38%) aligned exactly 1 time 14254876 (15.61%) aligned >1 times 96.99% overall alignment rate Time searching: 00:25:19 Overall time: 00:25:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 40 files... [bam_rmdupse_core] 27502625 / 88560007 = 0.3106 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 08:14:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7320031/SRX7320031.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7320031/SRX7320031.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7320031/SRX7320031.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7320031/SRX7320031.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 08:14:03: #1 read tag files... INFO @ Sat, 11 Dec 2021 08:14:03: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 08:14:09: 1000000 INFO @ Sat, 11 Dec 2021 08:14:15: 2000000 INFO @ Sat, 11 Dec 2021 08:14:21: 3000000 INFO @ Sat, 11 Dec 2021 08:14:26: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 08:14:32: 5000000 INFO @ Sat, 11 Dec 2021 08:14:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7320031/SRX7320031.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7320031/SRX7320031.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7320031/SRX7320031.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7320031/SRX7320031.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 08:14:33: #1 read tag files... INFO @ Sat, 11 Dec 2021 08:14:33: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 08:14:38: 1000000 INFO @ Sat, 11 Dec 2021 08:14:38: 6000000 INFO @ Sat, 11 Dec 2021 08:14:44: 2000000 INFO @ Sat, 11 Dec 2021 08:14:45: 7000000 INFO @ Sat, 11 Dec 2021 08:14:50: 3000000 INFO @ Sat, 11 Dec 2021 08:14:51: 8000000 INFO @ Sat, 11 Dec 2021 08:14:56: 4000000 INFO @ Sat, 11 Dec 2021 08:14:57: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 08:15:01: 5000000 INFO @ Sat, 11 Dec 2021 08:15:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7320031/SRX7320031.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7320031/SRX7320031.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7320031/SRX7320031.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7320031/SRX7320031.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 08:15:03: #1 read tag files... INFO @ Sat, 11 Dec 2021 08:15:03: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 08:15:03: 10000000 INFO @ Sat, 11 Dec 2021 08:15:07: 6000000 INFO @ Sat, 11 Dec 2021 08:15:09: 1000000 INFO @ Sat, 11 Dec 2021 08:15:09: 11000000 INFO @ Sat, 11 Dec 2021 08:15:13: 7000000 INFO @ Sat, 11 Dec 2021 08:15:14: 2000000 INFO @ Sat, 11 Dec 2021 08:15:15: 12000000 INFO @ Sat, 11 Dec 2021 08:15:18: 8000000 INFO @ Sat, 11 Dec 2021 08:15:20: 3000000 INFO @ Sat, 11 Dec 2021 08:15:22: 13000000 INFO @ Sat, 11 Dec 2021 08:15:24: 9000000 INFO @ Sat, 11 Dec 2021 08:15:26: 4000000 INFO @ Sat, 11 Dec 2021 08:15:28: 14000000 INFO @ Sat, 11 Dec 2021 08:15:30: 10000000 INFO @ Sat, 11 Dec 2021 08:15:32: 5000000 INFO @ Sat, 11 Dec 2021 08:15:34: 15000000 INFO @ Sat, 11 Dec 2021 08:15:35: 11000000 INFO @ Sat, 11 Dec 2021 08:15:38: 6000000 INFO @ Sat, 11 Dec 2021 08:15:40: 16000000 INFO @ Sat, 11 Dec 2021 08:15:41: 12000000 INFO @ Sat, 11 Dec 2021 08:15:43: 7000000 INFO @ Sat, 11 Dec 2021 08:15:46: 17000000 INFO @ Sat, 11 Dec 2021 08:15:47: 13000000 INFO @ Sat, 11 Dec 2021 08:15:49: 8000000 INFO @ Sat, 11 Dec 2021 08:15:52: 18000000 INFO @ Sat, 11 Dec 2021 08:15:52: 14000000 INFO @ Sat, 11 Dec 2021 08:15:55: 9000000 INFO @ Sat, 11 Dec 2021 08:15:58: 15000000 INFO @ Sat, 11 Dec 2021 08:15:58: 19000000 INFO @ Sat, 11 Dec 2021 08:16:01: 10000000 INFO @ Sat, 11 Dec 2021 08:16:04: 16000000 INFO @ Sat, 11 Dec 2021 08:16:04: 20000000 INFO @ Sat, 11 Dec 2021 08:16:06: 11000000 INFO @ Sat, 11 Dec 2021 08:16:09: 17000000 INFO @ Sat, 11 Dec 2021 08:16:11: 21000000 INFO @ Sat, 11 Dec 2021 08:16:12: 12000000 INFO @ Sat, 11 Dec 2021 08:16:15: 18000000 INFO @ Sat, 11 Dec 2021 08:16:17: 22000000 INFO @ Sat, 11 Dec 2021 08:16:18: 13000000 INFO @ Sat, 11 Dec 2021 08:16:21: 19000000 INFO @ Sat, 11 Dec 2021 08:16:23: 23000000 INFO @ Sat, 11 Dec 2021 08:16:23: 14000000 INFO @ Sat, 11 Dec 2021 08:16:26: 20000000 INFO @ Sat, 11 Dec 2021 08:16:29: 15000000 INFO @ Sat, 11 Dec 2021 08:16:29: 24000000 INFO @ Sat, 11 Dec 2021 08:16:32: 21000000 INFO @ Sat, 11 Dec 2021 08:16:35: 16000000 INFO @ Sat, 11 Dec 2021 08:16:36: 25000000 INFO @ Sat, 11 Dec 2021 08:16:38: 22000000 INFO @ Sat, 11 Dec 2021 08:16:40: 17000000 INFO @ Sat, 11 Dec 2021 08:16:42: 26000000 INFO @ Sat, 11 Dec 2021 08:16:43: 23000000 INFO @ Sat, 11 Dec 2021 08:16:46: 18000000 INFO @ Sat, 11 Dec 2021 08:16:48: 27000000 INFO @ Sat, 11 Dec 2021 08:16:49: 24000000 INFO @ Sat, 11 Dec 2021 08:16:52: 19000000 INFO @ Sat, 11 Dec 2021 08:16:54: 28000000 INFO @ Sat, 11 Dec 2021 08:16:55: 25000000 INFO @ Sat, 11 Dec 2021 08:16:58: 20000000 INFO @ Sat, 11 Dec 2021 08:17:00: 29000000 INFO @ Sat, 11 Dec 2021 08:17:01: 26000000 INFO @ Sat, 11 Dec 2021 08:17:04: 21000000 INFO @ Sat, 11 Dec 2021 08:17:06: 27000000 INFO @ Sat, 11 Dec 2021 08:17:07: 30000000 INFO @ Sat, 11 Dec 2021 08:17:10: 22000000 INFO @ Sat, 11 Dec 2021 08:17:12: 28000000 INFO @ Sat, 11 Dec 2021 08:17:13: 31000000 INFO @ Sat, 11 Dec 2021 08:17:16: 23000000 INFO @ Sat, 11 Dec 2021 08:17:18: 29000000 INFO @ Sat, 11 Dec 2021 08:17:19: 32000000 INFO @ Sat, 11 Dec 2021 08:17:21: 24000000 INFO @ Sat, 11 Dec 2021 08:17:24: 30000000 INFO @ Sat, 11 Dec 2021 08:17:25: 33000000 INFO @ Sat, 11 Dec 2021 08:17:27: 25000000 INFO @ Sat, 11 Dec 2021 08:17:30: 31000000 INFO @ Sat, 11 Dec 2021 08:17:31: 34000000 INFO @ Sat, 11 Dec 2021 08:17:33: 26000000 INFO @ Sat, 11 Dec 2021 08:17:35: 32000000 INFO @ Sat, 11 Dec 2021 08:17:37: 35000000 INFO @ Sat, 11 Dec 2021 08:17:39: 27000000 INFO @ Sat, 11 Dec 2021 08:17:41: 33000000 INFO @ Sat, 11 Dec 2021 08:17:44: 36000000 INFO @ Sat, 11 Dec 2021 08:17:45: 28000000 INFO @ Sat, 11 Dec 2021 08:17:47: 34000000 INFO @ Sat, 11 Dec 2021 08:17:50: 37000000 INFO @ Sat, 11 Dec 2021 08:17:51: 29000000 INFO @ Sat, 11 Dec 2021 08:17:52: 35000000 INFO @ Sat, 11 Dec 2021 08:17:56: 38000000 INFO @ Sat, 11 Dec 2021 08:17:57: 30000000 INFO @ Sat, 11 Dec 2021 08:17:58: 36000000 INFO @ Sat, 11 Dec 2021 08:18:02: 39000000 INFO @ Sat, 11 Dec 2021 08:18:03: 31000000 INFO @ Sat, 11 Dec 2021 08:18:04: 37000000 INFO @ Sat, 11 Dec 2021 08:18:09: 32000000 INFO @ Sat, 11 Dec 2021 08:18:09: 40000000 INFO @ Sat, 11 Dec 2021 08:18:10: 38000000 INFO @ Sat, 11 Dec 2021 08:18:14: 33000000 INFO @ Sat, 11 Dec 2021 08:18:15: 41000000 INFO @ Sat, 11 Dec 2021 08:18:16: 39000000 INFO @ Sat, 11 Dec 2021 08:18:20: 34000000 INFO @ Sat, 11 Dec 2021 08:18:21: 42000000 INFO @ Sat, 11 Dec 2021 08:18:21: 40000000 INFO @ Sat, 11 Dec 2021 08:18:26: 35000000 INFO @ Sat, 11 Dec 2021 08:18:27: 41000000 INFO @ Sat, 11 Dec 2021 08:18:27: 43000000 INFO @ Sat, 11 Dec 2021 08:18:32: 36000000 INFO @ Sat, 11 Dec 2021 08:18:33: 42000000 INFO @ Sat, 11 Dec 2021 08:18:33: 44000000 INFO @ Sat, 11 Dec 2021 08:18:38: 37000000 INFO @ Sat, 11 Dec 2021 08:18:39: 43000000 INFO @ Sat, 11 Dec 2021 08:18:40: 45000000 INFO @ Sat, 11 Dec 2021 08:18:44: 38000000 INFO @ Sat, 11 Dec 2021 08:18:44: 44000000 INFO @ Sat, 11 Dec 2021 08:18:46: 46000000 INFO @ Sat, 11 Dec 2021 08:18:50: 39000000 INFO @ Sat, 11 Dec 2021 08:18:50: 45000000 INFO @ Sat, 11 Dec 2021 08:18:52: 47000000 INFO @ Sat, 11 Dec 2021 08:18:56: 40000000 INFO @ Sat, 11 Dec 2021 08:18:56: 46000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 08:18:59: 48000000 INFO @ Sat, 11 Dec 2021 08:19:02: 41000000 INFO @ Sat, 11 Dec 2021 08:19:02: 47000000 INFO @ Sat, 11 Dec 2021 08:19:05: 49000000 INFO @ Sat, 11 Dec 2021 08:19:07: 42000000 INFO @ Sat, 11 Dec 2021 08:19:08: 48000000 INFO @ Sat, 11 Dec 2021 08:19:11: 50000000 INFO @ Sat, 11 Dec 2021 08:19:13: 43000000 INFO @ Sat, 11 Dec 2021 08:19:14: 49000000 INFO @ Sat, 11 Dec 2021 08:19:17: 51000000 INFO @ Sat, 11 Dec 2021 08:19:19: 44000000 INFO @ Sat, 11 Dec 2021 08:19:20: 50000000 INFO @ Sat, 11 Dec 2021 08:19:24: 52000000 INFO @ Sat, 11 Dec 2021 08:19:25: 45000000 INFO @ Sat, 11 Dec 2021 08:19:26: 51000000 INFO @ Sat, 11 Dec 2021 08:19:30: 53000000 INFO @ Sat, 11 Dec 2021 08:19:31: 46000000 INFO @ Sat, 11 Dec 2021 08:19:32: 52000000 INFO @ Sat, 11 Dec 2021 08:19:36: 54000000 INFO @ Sat, 11 Dec 2021 08:19:37: 47000000 INFO @ Sat, 11 Dec 2021 08:19:37: 53000000 INFO @ Sat, 11 Dec 2021 08:19:42: 55000000 INFO @ Sat, 11 Dec 2021 08:19:43: 48000000 INFO @ Sat, 11 Dec 2021 08:19:43: 54000000 INFO @ Sat, 11 Dec 2021 08:19:48: 56000000 INFO @ Sat, 11 Dec 2021 08:19:49: 55000000 INFO @ Sat, 11 Dec 2021 08:19:49: 49000000 INFO @ Sat, 11 Dec 2021 08:19:55: 56000000 INFO @ Sat, 11 Dec 2021 08:19:55: 57000000 INFO @ Sat, 11 Dec 2021 08:19:55: 50000000 INFO @ Sat, 11 Dec 2021 08:20:00: 57000000 INFO @ Sat, 11 Dec 2021 08:20:01: 58000000 INFO @ Sat, 11 Dec 2021 08:20:01: 51000000 INFO @ Sat, 11 Dec 2021 08:20:06: 58000000 INFO @ Sat, 11 Dec 2021 08:20:07: 59000000 INFO @ Sat, 11 Dec 2021 08:20:07: 52000000 INFO @ Sat, 11 Dec 2021 08:20:12: 59000000 INFO @ Sat, 11 Dec 2021 08:20:13: 53000000 INFO @ Sat, 11 Dec 2021 08:20:13: 60000000 INFO @ Sat, 11 Dec 2021 08:20:18: 60000000 INFO @ Sat, 11 Dec 2021 08:20:19: 54000000 INFO @ Sat, 11 Dec 2021 08:20:19: 61000000 INFO @ Sat, 11 Dec 2021 08:20:20: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 08:20:20: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 08:20:20: #1 total tags in treatment: 61057382 INFO @ Sat, 11 Dec 2021 08:20:20: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 08:20:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 08:20:21: #1 tags after filtering in treatment: 61057382 INFO @ Sat, 11 Dec 2021 08:20:21: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 08:20:21: #1 finished! INFO @ Sat, 11 Dec 2021 08:20:21: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 08:20:21: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 08:20:23: 61000000 INFO @ Sat, 11 Dec 2021 08:20:24: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 08:20:24: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 08:20:24: #1 total tags in treatment: 61057382 INFO @ Sat, 11 Dec 2021 08:20:24: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 08:20:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 08:20:24: 55000000 INFO @ Sat, 11 Dec 2021 08:20:25: #2 number of paired peaks: 0 WARNING @ Sat, 11 Dec 2021 08:20:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 08:20:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7320031/SRX7320031.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 6 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7320031/SRX7320031.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7320031/SRX7320031.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7320031/SRX7320031.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 08:20:25: #1 tags after filtering in treatment: 61057382 INFO @ Sat, 11 Dec 2021 08:20:25: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 08:20:25: #1 finished! INFO @ Sat, 11 Dec 2021 08:20:25: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 08:20:25: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 08:20:29: #2 number of paired peaks: 0 WARNING @ Sat, 11 Dec 2021 08:20:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 08:20:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7320031/SRX7320031.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7320031/SRX7320031.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7320031/SRX7320031.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7320031/SRX7320031.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 08:20:30: 56000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 08:20:35: 57000000 INFO @ Sat, 11 Dec 2021 08:20:39: 58000000 INFO @ Sat, 11 Dec 2021 08:20:45: 59000000 INFO @ Sat, 11 Dec 2021 08:20:50: 60000000 INFO @ Sat, 11 Dec 2021 08:20:54: 61000000 INFO @ Sat, 11 Dec 2021 08:20:55: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 08:20:55: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 08:20:55: #1 total tags in treatment: 61057382 INFO @ Sat, 11 Dec 2021 08:20:55: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 08:20:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 08:20:56: #1 tags after filtering in treatment: 61057382 INFO @ Sat, 11 Dec 2021 08:20:56: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 08:20:56: #1 finished! INFO @ Sat, 11 Dec 2021 08:20:56: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 08:20:56: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 08:21:00: #2 number of paired peaks: 0 WARNING @ Sat, 11 Dec 2021 08:21:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 08:21:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7320031/SRX7320031.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7320031/SRX7320031.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7320031/SRX7320031.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7320031/SRX7320031.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling