Job ID = 5721281 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 15,424,468 reads read : 15,424,468 reads written : 15,424,468 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:15 15424468 reads; of these: 15424468 (100.00%) were unpaired; of these: 784766 (5.09%) aligned 0 times 10660033 (69.11%) aligned exactly 1 time 3979669 (25.80%) aligned >1 times 94.91% overall alignment rate Time searching: 00:04:15 Overall time: 00:04:15 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 1759288 / 14639702 = 0.1202 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 06:24:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7299239/SRX7299239.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7299239/SRX7299239.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7299239/SRX7299239.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7299239/SRX7299239.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 06:24:36: #1 read tag files... INFO @ Thu, 16 Apr 2020 06:24:36: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 06:24:43: 1000000 INFO @ Thu, 16 Apr 2020 06:24:51: 2000000 INFO @ Thu, 16 Apr 2020 06:24:58: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 06:25:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7299239/SRX7299239.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7299239/SRX7299239.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7299239/SRX7299239.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7299239/SRX7299239.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 06:25:05: #1 read tag files... INFO @ Thu, 16 Apr 2020 06:25:05: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 06:25:05: 4000000 INFO @ Thu, 16 Apr 2020 06:25:11: 1000000 INFO @ Thu, 16 Apr 2020 06:25:13: 5000000 INFO @ Thu, 16 Apr 2020 06:25:18: 2000000 INFO @ Thu, 16 Apr 2020 06:25:20: 6000000 INFO @ Thu, 16 Apr 2020 06:25:24: 3000000 INFO @ Thu, 16 Apr 2020 06:25:27: 7000000 INFO @ Thu, 16 Apr 2020 06:25:31: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 06:25:33: 8000000 INFO @ Thu, 16 Apr 2020 06:25:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7299239/SRX7299239.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7299239/SRX7299239.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7299239/SRX7299239.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7299239/SRX7299239.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 06:25:36: #1 read tag files... INFO @ Thu, 16 Apr 2020 06:25:36: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 06:25:37: 5000000 INFO @ Thu, 16 Apr 2020 06:25:39: 9000000 INFO @ Thu, 16 Apr 2020 06:25:42: 1000000 INFO @ Thu, 16 Apr 2020 06:25:44: 6000000 INFO @ Thu, 16 Apr 2020 06:25:45: 10000000 INFO @ Thu, 16 Apr 2020 06:25:49: 2000000 INFO @ Thu, 16 Apr 2020 06:25:51: 7000000 INFO @ Thu, 16 Apr 2020 06:25:51: 11000000 INFO @ Thu, 16 Apr 2020 06:25:55: 3000000 INFO @ Thu, 16 Apr 2020 06:25:57: 8000000 INFO @ Thu, 16 Apr 2020 06:25:58: 12000000 INFO @ Thu, 16 Apr 2020 06:26:01: 4000000 INFO @ Thu, 16 Apr 2020 06:26:03: #1 tag size is determined as 35 bps INFO @ Thu, 16 Apr 2020 06:26:03: #1 tag size = 35 INFO @ Thu, 16 Apr 2020 06:26:03: #1 total tags in treatment: 12880414 INFO @ Thu, 16 Apr 2020 06:26:03: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 06:26:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 06:26:03: #1 tags after filtering in treatment: 12880414 INFO @ Thu, 16 Apr 2020 06:26:03: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 16 Apr 2020 06:26:03: #1 finished! INFO @ Thu, 16 Apr 2020 06:26:03: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 06:26:03: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 06:26:04: 9000000 INFO @ Thu, 16 Apr 2020 06:26:04: #2 number of paired peaks: 65 WARNING @ Thu, 16 Apr 2020 06:26:04: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 16 Apr 2020 06:26:04: Process for pairing-model is terminated! INFO @ Thu, 16 Apr 2020 06:26:08: 5000000 cut: /home/okishinya/chipatlas/results/dm3/SRX7299239/SRX7299239.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7299239/SRX7299239.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7299239/SRX7299239.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7299239/SRX7299239.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 06:26:11: 10000000 INFO @ Thu, 16 Apr 2020 06:26:14: 6000000 INFO @ Thu, 16 Apr 2020 06:26:17: 11000000 INFO @ Thu, 16 Apr 2020 06:26:20: 7000000 INFO @ Thu, 16 Apr 2020 06:26:23: 12000000 INFO @ Thu, 16 Apr 2020 06:26:26: 8000000 INFO @ Thu, 16 Apr 2020 06:26:29: #1 tag size is determined as 35 bps INFO @ Thu, 16 Apr 2020 06:26:29: #1 tag size = 35 INFO @ Thu, 16 Apr 2020 06:26:29: #1 total tags in treatment: 12880414 INFO @ Thu, 16 Apr 2020 06:26:29: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 06:26:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 06:26:29: #1 tags after filtering in treatment: 12880414 INFO @ Thu, 16 Apr 2020 06:26:29: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 16 Apr 2020 06:26:29: #1 finished! INFO @ Thu, 16 Apr 2020 06:26:29: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 06:26:29: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 06:26:30: #2 number of paired peaks: 65 WARNING @ Thu, 16 Apr 2020 06:26:30: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 16 Apr 2020 06:26:30: Process for pairing-model is terminated! INFO @ Thu, 16 Apr 2020 06:26:33: 9000000 cut: /home/okishinya/chipatlas/results/dm3/SRX7299239/SRX7299239.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7299239/SRX7299239.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7299239/SRX7299239.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7299239/SRX7299239.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 06:26:38: 10000000 INFO @ Thu, 16 Apr 2020 06:26:44: 11000000 INFO @ Thu, 16 Apr 2020 06:26:49: 12000000 INFO @ Thu, 16 Apr 2020 06:26:54: #1 tag size is determined as 35 bps INFO @ Thu, 16 Apr 2020 06:26:54: #1 tag size = 35 INFO @ Thu, 16 Apr 2020 06:26:54: #1 total tags in treatment: 12880414 INFO @ Thu, 16 Apr 2020 06:26:54: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 06:26:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 06:26:55: #1 tags after filtering in treatment: 12880414 INFO @ Thu, 16 Apr 2020 06:26:55: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 16 Apr 2020 06:26:55: #1 finished! INFO @ Thu, 16 Apr 2020 06:26:55: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 06:26:55: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 06:26:55: #2 number of paired peaks: 65 WARNING @ Thu, 16 Apr 2020 06:26:55: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 16 Apr 2020 06:26:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7299239/SRX7299239.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7299239/SRX7299239.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7299239/SRX7299239.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7299239/SRX7299239.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。