Job ID = 12266572 SRX = SRX7282922 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 5432514 spots for SRR10603281/SRR10603281.sra Written 5432514 spots for SRR10603281/SRR10603281.sra fastq に変換しました。 bowtie でマッピング中... Your job 12266733 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:04 5432514 reads; of these: 5432514 (100.00%) were unpaired; of these: 1153146 (21.23%) aligned 0 times 3845728 (70.79%) aligned exactly 1 time 433640 (7.98%) aligned >1 times 78.77% overall alignment rate Time searching: 00:01:04 Overall time: 00:01:04 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 1519740 / 4279368 = 0.3551 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 09:04:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7282922/SRX7282922.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7282922/SRX7282922.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7282922/SRX7282922.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7282922/SRX7282922.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 09:04:54: #1 read tag files... INFO @ Sat, 03 Apr 2021 09:04:54: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 09:05:03: 1000000 INFO @ Sat, 03 Apr 2021 09:05:12: 2000000 INFO @ Sat, 03 Apr 2021 09:05:18: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 09:05:18: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 09:05:18: #1 total tags in treatment: 2759628 INFO @ Sat, 03 Apr 2021 09:05:18: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 09:05:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 09:05:18: #1 tags after filtering in treatment: 2759628 INFO @ Sat, 03 Apr 2021 09:05:18: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 09:05:18: #1 finished! INFO @ Sat, 03 Apr 2021 09:05:18: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 09:05:18: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 09:05:19: #2 number of paired peaks: 8101 INFO @ Sat, 03 Apr 2021 09:05:19: start model_add_line... INFO @ Sat, 03 Apr 2021 09:05:19: start X-correlation... INFO @ Sat, 03 Apr 2021 09:05:19: end of X-cor INFO @ Sat, 03 Apr 2021 09:05:19: #2 finished! INFO @ Sat, 03 Apr 2021 09:05:19: #2 predicted fragment length is 106 bps INFO @ Sat, 03 Apr 2021 09:05:19: #2 alternative fragment length(s) may be 106 bps INFO @ Sat, 03 Apr 2021 09:05:19: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX7282922/SRX7282922.05_model.r INFO @ Sat, 03 Apr 2021 09:05:19: #3 Call peaks... INFO @ Sat, 03 Apr 2021 09:05:19: #3 Pre-compute pvalue-qvalue table... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 09:05:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7282922/SRX7282922.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7282922/SRX7282922.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7282922/SRX7282922.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7282922/SRX7282922.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 09:05:24: #1 read tag files... INFO @ Sat, 03 Apr 2021 09:05:24: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 09:05:25: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 09:05:29: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX7282922/SRX7282922.05_peaks.xls INFO @ Sat, 03 Apr 2021 09:05:29: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX7282922/SRX7282922.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 09:05:29: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX7282922/SRX7282922.05_summits.bed INFO @ Sat, 03 Apr 2021 09:05:29: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (13371 records, 4 fields): 18 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 09:05:34: 1000000 INFO @ Sat, 03 Apr 2021 09:05:44: 2000000 INFO @ Sat, 03 Apr 2021 09:05:52: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 09:05:52: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 09:05:52: #1 total tags in treatment: 2759628 INFO @ Sat, 03 Apr 2021 09:05:52: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 09:05:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 09:05:52: #1 tags after filtering in treatment: 2759628 INFO @ Sat, 03 Apr 2021 09:05:52: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 09:05:52: #1 finished! INFO @ Sat, 03 Apr 2021 09:05:52: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 09:05:52: #2 looking for paired plus/minus strand peaks... BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 09:05:52: #2 number of paired peaks: 8101 INFO @ Sat, 03 Apr 2021 09:05:52: start model_add_line... INFO @ Sat, 03 Apr 2021 09:05:52: start X-correlation... INFO @ Sat, 03 Apr 2021 09:05:52: end of X-cor INFO @ Sat, 03 Apr 2021 09:05:52: #2 finished! INFO @ Sat, 03 Apr 2021 09:05:52: #2 predicted fragment length is 106 bps INFO @ Sat, 03 Apr 2021 09:05:52: #2 alternative fragment length(s) may be 106 bps INFO @ Sat, 03 Apr 2021 09:05:52: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX7282922/SRX7282922.10_model.r INFO @ Sat, 03 Apr 2021 09:05:52: #3 Call peaks... INFO @ Sat, 03 Apr 2021 09:05:52: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 09:05:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7282922/SRX7282922.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7282922/SRX7282922.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7282922/SRX7282922.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7282922/SRX7282922.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 09:05:54: #1 read tag files... INFO @ Sat, 03 Apr 2021 09:05:54: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 09:05:59: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 09:06:02: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX7282922/SRX7282922.10_peaks.xls INFO @ Sat, 03 Apr 2021 09:06:02: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX7282922/SRX7282922.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 09:06:03: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX7282922/SRX7282922.10_summits.bed INFO @ Sat, 03 Apr 2021 09:06:03: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (8362 records, 4 fields): 11 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 09:06:03: 1000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 09:06:12: 2000000 BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 09:06:18: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 09:06:18: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 09:06:18: #1 total tags in treatment: 2759628 INFO @ Sat, 03 Apr 2021 09:06:18: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 09:06:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 09:06:18: #1 tags after filtering in treatment: 2759628 INFO @ Sat, 03 Apr 2021 09:06:18: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 09:06:18: #1 finished! INFO @ Sat, 03 Apr 2021 09:06:18: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 09:06:18: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 09:06:19: #2 number of paired peaks: 8101 INFO @ Sat, 03 Apr 2021 09:06:19: start model_add_line... INFO @ Sat, 03 Apr 2021 09:06:19: start X-correlation... INFO @ Sat, 03 Apr 2021 09:06:19: end of X-cor INFO @ Sat, 03 Apr 2021 09:06:19: #2 finished! INFO @ Sat, 03 Apr 2021 09:06:19: #2 predicted fragment length is 106 bps INFO @ Sat, 03 Apr 2021 09:06:19: #2 alternative fragment length(s) may be 106 bps INFO @ Sat, 03 Apr 2021 09:06:19: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX7282922/SRX7282922.20_model.r INFO @ Sat, 03 Apr 2021 09:06:19: #3 Call peaks... INFO @ Sat, 03 Apr 2021 09:06:19: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 09:06:25: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 09:06:29: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX7282922/SRX7282922.20_peaks.xls INFO @ Sat, 03 Apr 2021 09:06:29: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX7282922/SRX7282922.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 09:06:29: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX7282922/SRX7282922.20_summits.bed INFO @ Sat, 03 Apr 2021 09:06:29: Done! pass1 - making usageList (14 chroms): 1 millis pass2 - checking and writing primary data (3784 records, 4 fields): 7 millis CompletedMACS2peakCalling