Job ID = 12266571 SRX = SRX7282921 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 3324393 spots for SRR10603280/SRR10603280.sra Written 3324393 spots for SRR10603280/SRR10603280.sra fastq に変換しました。 bowtie でマッピング中... Your job 12266716 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:50 3324393 reads; of these: 3324393 (100.00%) were unpaired; of these: 583923 (17.56%) aligned 0 times 2415722 (72.67%) aligned exactly 1 time 324748 (9.77%) aligned >1 times 82.44% overall alignment rate Time searching: 00:00:50 Overall time: 00:00:50 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 609187 / 2740470 = 0.2223 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 09:03:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7282921/SRX7282921.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7282921/SRX7282921.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7282921/SRX7282921.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7282921/SRX7282921.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 09:03:56: #1 read tag files... INFO @ Sat, 03 Apr 2021 09:03:56: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 09:04:01: 1000000 INFO @ Sat, 03 Apr 2021 09:04:06: 2000000 INFO @ Sat, 03 Apr 2021 09:04:07: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 09:04:07: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 09:04:07: #1 total tags in treatment: 2131283 INFO @ Sat, 03 Apr 2021 09:04:07: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 09:04:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 09:04:07: #1 tags after filtering in treatment: 2131283 INFO @ Sat, 03 Apr 2021 09:04:07: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 09:04:07: #1 finished! INFO @ Sat, 03 Apr 2021 09:04:07: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 09:04:07: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 09:04:07: #2 number of paired peaks: 5989 INFO @ Sat, 03 Apr 2021 09:04:07: start model_add_line... INFO @ Sat, 03 Apr 2021 09:04:07: start X-correlation... INFO @ Sat, 03 Apr 2021 09:04:07: end of X-cor INFO @ Sat, 03 Apr 2021 09:04:07: #2 finished! INFO @ Sat, 03 Apr 2021 09:04:07: #2 predicted fragment length is 103 bps INFO @ Sat, 03 Apr 2021 09:04:07: #2 alternative fragment length(s) may be 103 bps INFO @ Sat, 03 Apr 2021 09:04:07: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX7282921/SRX7282921.05_model.r INFO @ Sat, 03 Apr 2021 09:04:07: #3 Call peaks... INFO @ Sat, 03 Apr 2021 09:04:07: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 09:04:12: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 09:04:15: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX7282921/SRX7282921.05_peaks.xls INFO @ Sat, 03 Apr 2021 09:04:15: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX7282921/SRX7282921.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 09:04:16: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX7282921/SRX7282921.05_summits.bed INFO @ Sat, 03 Apr 2021 09:04:16: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (9199 records, 4 fields): 11 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 09:04:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7282921/SRX7282921.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7282921/SRX7282921.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7282921/SRX7282921.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7282921/SRX7282921.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 09:04:26: #1 read tag files... INFO @ Sat, 03 Apr 2021 09:04:26: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 09:04:31: 1000000 INFO @ Sat, 03 Apr 2021 09:04:37: 2000000 INFO @ Sat, 03 Apr 2021 09:04:37: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 09:04:37: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 09:04:37: #1 total tags in treatment: 2131283 INFO @ Sat, 03 Apr 2021 09:04:37: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 09:04:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 09:04:37: #1 tags after filtering in treatment: 2131283 INFO @ Sat, 03 Apr 2021 09:04:37: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 09:04:37: #1 finished! INFO @ Sat, 03 Apr 2021 09:04:37: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 09:04:37: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 09:04:38: #2 number of paired peaks: 5989 INFO @ Sat, 03 Apr 2021 09:04:38: start model_add_line... INFO @ Sat, 03 Apr 2021 09:04:38: start X-correlation... INFO @ Sat, 03 Apr 2021 09:04:38: end of X-cor INFO @ Sat, 03 Apr 2021 09:04:38: #2 finished! INFO @ Sat, 03 Apr 2021 09:04:38: #2 predicted fragment length is 103 bps INFO @ Sat, 03 Apr 2021 09:04:38: #2 alternative fragment length(s) may be 103 bps INFO @ Sat, 03 Apr 2021 09:04:38: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX7282921/SRX7282921.10_model.r INFO @ Sat, 03 Apr 2021 09:04:38: #3 Call peaks... INFO @ Sat, 03 Apr 2021 09:04:38: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 09:04:43: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 09:04:46: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX7282921/SRX7282921.10_peaks.xls INFO @ Sat, 03 Apr 2021 09:04:46: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX7282921/SRX7282921.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 09:04:46: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX7282921/SRX7282921.10_summits.bed INFO @ Sat, 03 Apr 2021 09:04:46: Done! pass1 - making usageList (14 chroms): 1 millis pass2 - checking and writing primary data (5180 records, 4 fields): 7 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 09:04:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7282921/SRX7282921.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7282921/SRX7282921.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7282921/SRX7282921.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7282921/SRX7282921.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 09:04:56: #1 read tag files... INFO @ Sat, 03 Apr 2021 09:04:56: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 09:05:01: 1000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 09:05:06: 2000000 INFO @ Sat, 03 Apr 2021 09:05:07: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 09:05:07: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 09:05:07: #1 total tags in treatment: 2131283 INFO @ Sat, 03 Apr 2021 09:05:07: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 09:05:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 09:05:07: #1 tags after filtering in treatment: 2131283 INFO @ Sat, 03 Apr 2021 09:05:07: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 09:05:07: #1 finished! INFO @ Sat, 03 Apr 2021 09:05:07: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 09:05:07: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 09:05:08: #2 number of paired peaks: 5989 INFO @ Sat, 03 Apr 2021 09:05:08: start model_add_line... INFO @ Sat, 03 Apr 2021 09:05:08: start X-correlation... INFO @ Sat, 03 Apr 2021 09:05:08: end of X-cor INFO @ Sat, 03 Apr 2021 09:05:08: #2 finished! INFO @ Sat, 03 Apr 2021 09:05:08: #2 predicted fragment length is 103 bps INFO @ Sat, 03 Apr 2021 09:05:08: #2 alternative fragment length(s) may be 103 bps INFO @ Sat, 03 Apr 2021 09:05:08: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX7282921/SRX7282921.20_model.r INFO @ Sat, 03 Apr 2021 09:05:08: #3 Call peaks... INFO @ Sat, 03 Apr 2021 09:05:08: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 09:05:13: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 09:05:16: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX7282921/SRX7282921.20_peaks.xls INFO @ Sat, 03 Apr 2021 09:05:16: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX7282921/SRX7282921.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 09:05:16: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX7282921/SRX7282921.20_summits.bed INFO @ Sat, 03 Apr 2021 09:05:16: Done! pass1 - making usageList (11 chroms): 0 millis pass2 - checking and writing primary data (2009 records, 4 fields): 4 millis CompletedMACS2peakCalling