Job ID = 12266387 SRX = SRX7282799 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 24281 spots for SRR10603038/SRR10603038.sra Written 24281 spots for SRR10603038/SRR10603038.sra fastq に変換しました。 bowtie でマッピング中... Your job 12266498 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:01 24281 reads; of these: 24281 (100.00%) were unpaired; of these: 2966 (12.22%) aligned 0 times 9609 (39.57%) aligned exactly 1 time 11706 (48.21%) aligned >1 times 87.78% overall alignment rate Time searching: 00:00:01 Overall time: 00:00:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 12633 / 21315 = 0.5927 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:57:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7282799/SRX7282799.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7282799/SRX7282799.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7282799/SRX7282799.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7282799/SRX7282799.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:57:34: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:57:34: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:57:34: #1 tag size is determined as 54 bps INFO @ Sat, 03 Apr 2021 08:57:34: #1 tag size = 54 INFO @ Sat, 03 Apr 2021 08:57:34: #1 total tags in treatment: 8682 INFO @ Sat, 03 Apr 2021 08:57:34: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:57:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:57:34: #1 tags after filtering in treatment: 8680 INFO @ Sat, 03 Apr 2021 08:57:34: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 08:57:34: #1 finished! INFO @ Sat, 03 Apr 2021 08:57:34: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:57:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:57:34: #2 number of paired peaks: 201 WARNING @ Sat, 03 Apr 2021 08:57:34: Fewer paired peaks (201) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 201 pairs to build model! INFO @ Sat, 03 Apr 2021 08:57:34: start model_add_line... INFO @ Sat, 03 Apr 2021 08:57:34: start X-correlation... INFO @ Sat, 03 Apr 2021 08:57:34: end of X-cor INFO @ Sat, 03 Apr 2021 08:57:34: #2 finished! INFO @ Sat, 03 Apr 2021 08:57:34: #2 predicted fragment length is 9 bps INFO @ Sat, 03 Apr 2021 08:57:34: #2 alternative fragment length(s) may be 9,51,87,132,145,170,190,230,266,288,325,362,407,508,567 bps INFO @ Sat, 03 Apr 2021 08:57:34: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX7282799/SRX7282799.05_model.r WARNING @ Sat, 03 Apr 2021 08:57:34: #2 Since the d (9) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 08:57:34: #2 You may need to consider one of the other alternative d(s): 9,51,87,132,145,170,190,230,266,288,325,362,407,508,567 WARNING @ Sat, 03 Apr 2021 08:57:34: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 08:57:34: #3 Call peaks... INFO @ Sat, 03 Apr 2021 08:57:34: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 08:57:34: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 08:57:34: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX7282799/SRX7282799.05_peaks.xls INFO @ Sat, 03 Apr 2021 08:57:34: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX7282799/SRX7282799.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 08:57:34: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX7282799/SRX7282799.05_summits.bed INFO @ Sat, 03 Apr 2021 08:57:34: Done! pass1 - making usageList (1 chroms): 1 millis pass2 - checking and writing primary data (6 records, 4 fields): 0 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:58:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7282799/SRX7282799.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7282799/SRX7282799.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7282799/SRX7282799.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7282799/SRX7282799.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:58:04: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:58:04: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:58:04: #1 tag size is determined as 54 bps INFO @ Sat, 03 Apr 2021 08:58:04: #1 tag size = 54 INFO @ Sat, 03 Apr 2021 08:58:04: #1 total tags in treatment: 8682 INFO @ Sat, 03 Apr 2021 08:58:04: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:58:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:58:04: #1 tags after filtering in treatment: 8680 INFO @ Sat, 03 Apr 2021 08:58:04: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 08:58:04: #1 finished! INFO @ Sat, 03 Apr 2021 08:58:04: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:58:04: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:58:04: #2 number of paired peaks: 201 WARNING @ Sat, 03 Apr 2021 08:58:04: Fewer paired peaks (201) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 201 pairs to build model! INFO @ Sat, 03 Apr 2021 08:58:04: start model_add_line... INFO @ Sat, 03 Apr 2021 08:58:04: start X-correlation... INFO @ Sat, 03 Apr 2021 08:58:04: end of X-cor INFO @ Sat, 03 Apr 2021 08:58:04: #2 finished! INFO @ Sat, 03 Apr 2021 08:58:04: #2 predicted fragment length is 9 bps INFO @ Sat, 03 Apr 2021 08:58:04: #2 alternative fragment length(s) may be 9,51,87,132,145,170,190,230,266,288,325,362,407,508,567 bps INFO @ Sat, 03 Apr 2021 08:58:04: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX7282799/SRX7282799.10_model.r WARNING @ Sat, 03 Apr 2021 08:58:04: #2 Since the d (9) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 08:58:04: #2 You may need to consider one of the other alternative d(s): 9,51,87,132,145,170,190,230,266,288,325,362,407,508,567 WARNING @ Sat, 03 Apr 2021 08:58:04: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 08:58:04: #3 Call peaks... INFO @ Sat, 03 Apr 2021 08:58:04: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 08:58:04: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 08:58:04: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX7282799/SRX7282799.10_peaks.xls INFO @ Sat, 03 Apr 2021 08:58:04: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX7282799/SRX7282799.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 08:58:04: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX7282799/SRX7282799.10_summits.bed INFO @ Sat, 03 Apr 2021 08:58:04: Done! pass1 - making usageList (1 chroms): 0 millis pass2 - checking and writing primary data (1 records, 4 fields): 1 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 08:58:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7282799/SRX7282799.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7282799/SRX7282799.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7282799/SRX7282799.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7282799/SRX7282799.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:58:34: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:58:34: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:58:34: #1 tag size is determined as 54 bps INFO @ Sat, 03 Apr 2021 08:58:34: #1 tag size = 54 INFO @ Sat, 03 Apr 2021 08:58:34: #1 total tags in treatment: 8682 INFO @ Sat, 03 Apr 2021 08:58:34: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:58:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:58:34: #1 tags after filtering in treatment: 8680 INFO @ Sat, 03 Apr 2021 08:58:34: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 08:58:34: #1 finished! INFO @ Sat, 03 Apr 2021 08:58:34: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:58:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:58:34: #2 number of paired peaks: 201 WARNING @ Sat, 03 Apr 2021 08:58:34: Fewer paired peaks (201) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 201 pairs to build model! INFO @ Sat, 03 Apr 2021 08:58:34: start model_add_line... INFO @ Sat, 03 Apr 2021 08:58:34: start X-correlation... INFO @ Sat, 03 Apr 2021 08:58:34: end of X-cor INFO @ Sat, 03 Apr 2021 08:58:34: #2 finished! INFO @ Sat, 03 Apr 2021 08:58:34: #2 predicted fragment length is 9 bps INFO @ Sat, 03 Apr 2021 08:58:34: #2 alternative fragment length(s) may be 9,51,87,132,145,170,190,230,266,288,325,362,407,508,567 bps INFO @ Sat, 03 Apr 2021 08:58:34: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX7282799/SRX7282799.20_model.r WARNING @ Sat, 03 Apr 2021 08:58:34: #2 Since the d (9) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 08:58:34: #2 You may need to consider one of the other alternative d(s): 9,51,87,132,145,170,190,230,266,288,325,362,407,508,567 WARNING @ Sat, 03 Apr 2021 08:58:34: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 08:58:34: #3 Call peaks... INFO @ Sat, 03 Apr 2021 08:58:34: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 08:58:34: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 08:58:34: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX7282799/SRX7282799.20_peaks.xls INFO @ Sat, 03 Apr 2021 08:58:34: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX7282799/SRX7282799.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 08:58:34: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX7282799/SRX7282799.20_summits.bed INFO @ Sat, 03 Apr 2021 08:58:34: Done! pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling