Job ID = 12266202 SRX = SRX7282721 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 57239 spots for SRR10603152/SRR10603152.sra Written 57239 spots for SRR10603152/SRR10603152.sra fastq に変換しました。 bowtie でマッピング中... Your job 12266311 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:02 57239 reads; of these: 57239 (100.00%) were unpaired; of these: 8580 (14.99%) aligned 0 times 27878 (48.70%) aligned exactly 1 time 20781 (36.31%) aligned >1 times 85.01% overall alignment rate Time searching: 00:00:02 Overall time: 00:00:02 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 24662 / 48659 = 0.5068 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:51:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7282721/SRX7282721.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7282721/SRX7282721.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7282721/SRX7282721.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7282721/SRX7282721.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:51:33: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:51:33: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:51:34: #1 tag size is determined as 54 bps INFO @ Sat, 03 Apr 2021 08:51:34: #1 tag size = 54 INFO @ Sat, 03 Apr 2021 08:51:34: #1 total tags in treatment: 23997 INFO @ Sat, 03 Apr 2021 08:51:34: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:51:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:51:34: #1 tags after filtering in treatment: 23997 INFO @ Sat, 03 Apr 2021 08:51:34: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 08:51:34: #1 finished! INFO @ Sat, 03 Apr 2021 08:51:34: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:51:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:51:34: #2 number of paired peaks: 905 WARNING @ Sat, 03 Apr 2021 08:51:34: Fewer paired peaks (905) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 905 pairs to build model! INFO @ Sat, 03 Apr 2021 08:51:34: start model_add_line... INFO @ Sat, 03 Apr 2021 08:51:34: start X-correlation... INFO @ Sat, 03 Apr 2021 08:51:34: end of X-cor INFO @ Sat, 03 Apr 2021 08:51:34: #2 finished! INFO @ Sat, 03 Apr 2021 08:51:34: #2 predicted fragment length is 10 bps INFO @ Sat, 03 Apr 2021 08:51:34: #2 alternative fragment length(s) may be 10,63,103,129,184,236,258,281,296,512,551 bps INFO @ Sat, 03 Apr 2021 08:51:34: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX7282721/SRX7282721.05_model.r WARNING @ Sat, 03 Apr 2021 08:51:34: #2 Since the d (10) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 08:51:34: #2 You may need to consider one of the other alternative d(s): 10,63,103,129,184,236,258,281,296,512,551 WARNING @ Sat, 03 Apr 2021 08:51:34: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 08:51:34: #3 Call peaks... INFO @ Sat, 03 Apr 2021 08:51:34: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 08:51:34: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 08:51:34: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX7282721/SRX7282721.05_peaks.xls INFO @ Sat, 03 Apr 2021 08:51:34: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX7282721/SRX7282721.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 08:51:34: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX7282721/SRX7282721.05_summits.bed INFO @ Sat, 03 Apr 2021 08:51:34: Done! pass1 - making usageList (1 chroms): 1 millis pass2 - checking and writing primary data (7 records, 4 fields): 1 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:52:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7282721/SRX7282721.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7282721/SRX7282721.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7282721/SRX7282721.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7282721/SRX7282721.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:52:03: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:52:03: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:52:04: #1 tag size is determined as 54 bps INFO @ Sat, 03 Apr 2021 08:52:04: #1 tag size = 54 INFO @ Sat, 03 Apr 2021 08:52:04: #1 total tags in treatment: 23997 INFO @ Sat, 03 Apr 2021 08:52:04: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:52:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:52:04: #1 tags after filtering in treatment: 23997 INFO @ Sat, 03 Apr 2021 08:52:04: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 08:52:04: #1 finished! INFO @ Sat, 03 Apr 2021 08:52:04: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:52:04: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:52:04: #2 number of paired peaks: 905 WARNING @ Sat, 03 Apr 2021 08:52:04: Fewer paired peaks (905) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 905 pairs to build model! INFO @ Sat, 03 Apr 2021 08:52:04: start model_add_line... INFO @ Sat, 03 Apr 2021 08:52:04: start X-correlation... INFO @ Sat, 03 Apr 2021 08:52:04: end of X-cor INFO @ Sat, 03 Apr 2021 08:52:04: #2 finished! INFO @ Sat, 03 Apr 2021 08:52:04: #2 predicted fragment length is 10 bps INFO @ Sat, 03 Apr 2021 08:52:04: #2 alternative fragment length(s) may be 10,63,103,129,184,236,258,281,296,512,551 bps INFO @ Sat, 03 Apr 2021 08:52:04: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX7282721/SRX7282721.10_model.r WARNING @ Sat, 03 Apr 2021 08:52:04: #2 Since the d (10) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 08:52:04: #2 You may need to consider one of the other alternative d(s): 10,63,103,129,184,236,258,281,296,512,551 WARNING @ Sat, 03 Apr 2021 08:52:04: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 08:52:04: #3 Call peaks... INFO @ Sat, 03 Apr 2021 08:52:04: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 08:52:04: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 08:52:04: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX7282721/SRX7282721.10_peaks.xls INFO @ Sat, 03 Apr 2021 08:52:04: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX7282721/SRX7282721.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 08:52:04: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX7282721/SRX7282721.10_summits.bed INFO @ Sat, 03 Apr 2021 08:52:04: Done! pass1 - making usageList (1 chroms): 1 millis pass2 - checking and writing primary data (2 records, 4 fields): 1 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 08:52:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7282721/SRX7282721.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7282721/SRX7282721.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7282721/SRX7282721.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7282721/SRX7282721.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:52:33: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:52:33: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:52:34: #1 tag size is determined as 54 bps INFO @ Sat, 03 Apr 2021 08:52:34: #1 tag size = 54 INFO @ Sat, 03 Apr 2021 08:52:34: #1 total tags in treatment: 23997 INFO @ Sat, 03 Apr 2021 08:52:34: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:52:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:52:34: #1 tags after filtering in treatment: 23997 INFO @ Sat, 03 Apr 2021 08:52:34: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 08:52:34: #1 finished! INFO @ Sat, 03 Apr 2021 08:52:34: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:52:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:52:34: #2 number of paired peaks: 905 WARNING @ Sat, 03 Apr 2021 08:52:34: Fewer paired peaks (905) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 905 pairs to build model! INFO @ Sat, 03 Apr 2021 08:52:34: start model_add_line... INFO @ Sat, 03 Apr 2021 08:52:34: start X-correlation... INFO @ Sat, 03 Apr 2021 08:52:34: end of X-cor INFO @ Sat, 03 Apr 2021 08:52:34: #2 finished! INFO @ Sat, 03 Apr 2021 08:52:34: #2 predicted fragment length is 10 bps INFO @ Sat, 03 Apr 2021 08:52:34: #2 alternative fragment length(s) may be 10,63,103,129,184,236,258,281,296,512,551 bps INFO @ Sat, 03 Apr 2021 08:52:34: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX7282721/SRX7282721.20_model.r WARNING @ Sat, 03 Apr 2021 08:52:34: #2 Since the d (10) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 08:52:34: #2 You may need to consider one of the other alternative d(s): 10,63,103,129,184,236,258,281,296,512,551 WARNING @ Sat, 03 Apr 2021 08:52:34: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 08:52:34: #3 Call peaks... INFO @ Sat, 03 Apr 2021 08:52:34: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 08:52:34: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 08:52:34: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX7282721/SRX7282721.20_peaks.xls INFO @ Sat, 03 Apr 2021 08:52:34: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX7282721/SRX7282721.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 08:52:34: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX7282721/SRX7282721.20_summits.bed INFO @ Sat, 03 Apr 2021 08:52:34: Done! pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling