Job ID = 12266073 SRX = SRX7282670 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 4945 spots for SRR10603085/SRR10603085.sra Written 4945 spots for SRR10603085/SRR10603085.sra fastq に変換しました。 bowtie でマッピング中... Your job 12266181 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:00 4945 reads; of these: 4945 (100.00%) were unpaired; of these: 501 (10.13%) aligned 0 times 147 (2.97%) aligned exactly 1 time 4297 (86.90%) aligned >1 times 89.87% overall alignment rate Time searching: 00:00:00 Overall time: 00:00:00 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 3682 / 4444 = 0.8285 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:46:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7282670/SRX7282670.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7282670/SRX7282670.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7282670/SRX7282670.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7282670/SRX7282670.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:46:36: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:46:36: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:46:36: #1 tag size is determined as 54 bps INFO @ Sat, 03 Apr 2021 08:46:36: #1 tag size = 54 INFO @ Sat, 03 Apr 2021 08:46:36: #1 total tags in treatment: 762 INFO @ Sat, 03 Apr 2021 08:46:36: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:46:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:46:36: #1 tags after filtering in treatment: 758 INFO @ Sat, 03 Apr 2021 08:46:36: #1 Redundant rate of treatment: 0.01 INFO @ Sat, 03 Apr 2021 08:46:36: #1 finished! INFO @ Sat, 03 Apr 2021 08:46:36: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:46:36: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:46:36: #2 number of paired peaks: 0 WARNING @ Sat, 03 Apr 2021 08:46:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 08:46:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7282670/SRX7282670.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7282670/SRX7282670.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7282670/SRX7282670.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7282670/SRX7282670.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:47:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7282670/SRX7282670.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7282670/SRX7282670.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7282670/SRX7282670.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7282670/SRX7282670.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:47:06: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:47:06: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:47:06: #1 tag size is determined as 54 bps INFO @ Sat, 03 Apr 2021 08:47:06: #1 tag size = 54 INFO @ Sat, 03 Apr 2021 08:47:06: #1 total tags in treatment: 762 INFO @ Sat, 03 Apr 2021 08:47:06: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:47:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:47:06: #1 tags after filtering in treatment: 758 INFO @ Sat, 03 Apr 2021 08:47:06: #1 Redundant rate of treatment: 0.01 INFO @ Sat, 03 Apr 2021 08:47:06: #1 finished! INFO @ Sat, 03 Apr 2021 08:47:06: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:47:06: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:47:06: #2 number of paired peaks: 0 WARNING @ Sat, 03 Apr 2021 08:47:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 08:47:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7282670/SRX7282670.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7282670/SRX7282670.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7282670/SRX7282670.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7282670/SRX7282670.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 08:47:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7282670/SRX7282670.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7282670/SRX7282670.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7282670/SRX7282670.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7282670/SRX7282670.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:47:36: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:47:36: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:47:36: #1 tag size is determined as 54 bps INFO @ Sat, 03 Apr 2021 08:47:36: #1 tag size = 54 INFO @ Sat, 03 Apr 2021 08:47:36: #1 total tags in treatment: 762 INFO @ Sat, 03 Apr 2021 08:47:36: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:47:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:47:36: #1 tags after filtering in treatment: 758 INFO @ Sat, 03 Apr 2021 08:47:36: #1 Redundant rate of treatment: 0.01 INFO @ Sat, 03 Apr 2021 08:47:36: #1 finished! INFO @ Sat, 03 Apr 2021 08:47:36: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:47:36: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:47:36: #2 number of paired peaks: 0 WARNING @ Sat, 03 Apr 2021 08:47:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 08:47:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7282670/SRX7282670.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7282670/SRX7282670.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7282670/SRX7282670.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7282670/SRX7282670.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling