Job ID = 12265682 SRX = SRX7281006 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 20323181 spots for SRR10601381/SRR10601381.sra Written 20323181 spots for SRR10601381/SRR10601381.sra fastq に変換しました。 bowtie でマッピング中... Your job 12265821 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:40 20323181 reads; of these: 20323181 (100.00%) were unpaired; of these: 7188104 (35.37%) aligned 0 times 9798103 (48.21%) aligned exactly 1 time 3336974 (16.42%) aligned >1 times 64.63% overall alignment rate Time searching: 00:06:40 Overall time: 00:06:40 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 7852358 / 13135077 = 0.5978 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:19:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7281006/SRX7281006.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7281006/SRX7281006.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7281006/SRX7281006.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7281006/SRX7281006.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:19:57: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:19:57: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:20:03: 1000000 INFO @ Sat, 03 Apr 2021 08:20:09: 2000000 INFO @ Sat, 03 Apr 2021 08:20:14: 3000000 INFO @ Sat, 03 Apr 2021 08:20:20: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:20:25: 5000000 INFO @ Sat, 03 Apr 2021 08:20:27: #1 tag size is determined as 74 bps INFO @ Sat, 03 Apr 2021 08:20:27: #1 tag size = 74 INFO @ Sat, 03 Apr 2021 08:20:27: #1 total tags in treatment: 5282719 INFO @ Sat, 03 Apr 2021 08:20:27: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:20:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:20:27: #1 tags after filtering in treatment: 5282719 INFO @ Sat, 03 Apr 2021 08:20:27: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 08:20:27: #1 finished! INFO @ Sat, 03 Apr 2021 08:20:27: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:20:27: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:20:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7281006/SRX7281006.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7281006/SRX7281006.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7281006/SRX7281006.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7281006/SRX7281006.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:20:27: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:20:27: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:20:28: #2 number of paired peaks: 6973 INFO @ Sat, 03 Apr 2021 08:20:28: start model_add_line... INFO @ Sat, 03 Apr 2021 08:20:28: start X-correlation... INFO @ Sat, 03 Apr 2021 08:20:28: end of X-cor INFO @ Sat, 03 Apr 2021 08:20:28: #2 finished! INFO @ Sat, 03 Apr 2021 08:20:28: #2 predicted fragment length is 97 bps INFO @ Sat, 03 Apr 2021 08:20:28: #2 alternative fragment length(s) may be 97 bps INFO @ Sat, 03 Apr 2021 08:20:28: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX7281006/SRX7281006.05_model.r WARNING @ Sat, 03 Apr 2021 08:20:28: #2 Since the d (97) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 08:20:28: #2 You may need to consider one of the other alternative d(s): 97 WARNING @ Sat, 03 Apr 2021 08:20:28: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 08:20:28: #3 Call peaks... INFO @ Sat, 03 Apr 2021 08:20:28: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 08:20:33: 1000000 INFO @ Sat, 03 Apr 2021 08:20:38: 2000000 INFO @ Sat, 03 Apr 2021 08:20:39: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 08:20:44: 3000000 INFO @ Sat, 03 Apr 2021 08:20:46: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX7281006/SRX7281006.05_peaks.xls INFO @ Sat, 03 Apr 2021 08:20:46: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX7281006/SRX7281006.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 08:20:46: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX7281006/SRX7281006.05_summits.bed INFO @ Sat, 03 Apr 2021 08:20:46: Done! pass1 - making usageList (14 chroms): 4 millis pass2 - checking and writing primary data (18521 records, 4 fields): 22 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 08:20:49: 4000000 BedGraph に変換中... INFO @ Sat, 03 Apr 2021 08:20:55: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:20:57: #1 tag size is determined as 74 bps INFO @ Sat, 03 Apr 2021 08:20:57: #1 tag size = 74 INFO @ Sat, 03 Apr 2021 08:20:57: #1 total tags in treatment: 5282719 INFO @ Sat, 03 Apr 2021 08:20:57: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:20:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:20:57: #1 tags after filtering in treatment: 5282719 INFO @ Sat, 03 Apr 2021 08:20:57: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 08:20:57: #1 finished! INFO @ Sat, 03 Apr 2021 08:20:57: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:20:57: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:20:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7281006/SRX7281006.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7281006/SRX7281006.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7281006/SRX7281006.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7281006/SRX7281006.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:20:57: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:20:57: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:20:58: #2 number of paired peaks: 6973 INFO @ Sat, 03 Apr 2021 08:20:58: start model_add_line... INFO @ Sat, 03 Apr 2021 08:20:58: start X-correlation... INFO @ Sat, 03 Apr 2021 08:20:58: end of X-cor INFO @ Sat, 03 Apr 2021 08:20:58: #2 finished! INFO @ Sat, 03 Apr 2021 08:20:58: #2 predicted fragment length is 97 bps INFO @ Sat, 03 Apr 2021 08:20:58: #2 alternative fragment length(s) may be 97 bps INFO @ Sat, 03 Apr 2021 08:20:58: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX7281006/SRX7281006.10_model.r WARNING @ Sat, 03 Apr 2021 08:20:58: #2 Since the d (97) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 08:20:58: #2 You may need to consider one of the other alternative d(s): 97 WARNING @ Sat, 03 Apr 2021 08:20:58: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 08:20:58: #3 Call peaks... INFO @ Sat, 03 Apr 2021 08:20:58: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 08:21:03: 1000000 INFO @ Sat, 03 Apr 2021 08:21:09: 2000000 INFO @ Sat, 03 Apr 2021 08:21:09: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 08:21:14: 3000000 INFO @ Sat, 03 Apr 2021 08:21:15: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX7281006/SRX7281006.10_peaks.xls INFO @ Sat, 03 Apr 2021 08:21:15: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX7281006/SRX7281006.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 08:21:15: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX7281006/SRX7281006.10_summits.bed INFO @ Sat, 03 Apr 2021 08:21:15: Done! pass1 - making usageList (14 chroms): 3 millis pass2 - checking and writing primary data (12550 records, 4 fields): 16 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 08:21:20: 4000000 INFO @ Sat, 03 Apr 2021 08:21:25: 5000000 INFO @ Sat, 03 Apr 2021 08:21:27: #1 tag size is determined as 74 bps INFO @ Sat, 03 Apr 2021 08:21:27: #1 tag size = 74 INFO @ Sat, 03 Apr 2021 08:21:27: #1 total tags in treatment: 5282719 INFO @ Sat, 03 Apr 2021 08:21:27: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:21:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:21:27: #1 tags after filtering in treatment: 5282719 INFO @ Sat, 03 Apr 2021 08:21:27: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 08:21:27: #1 finished! INFO @ Sat, 03 Apr 2021 08:21:27: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:21:27: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:21:28: #2 number of paired peaks: 6973 INFO @ Sat, 03 Apr 2021 08:21:28: start model_add_line... INFO @ Sat, 03 Apr 2021 08:21:28: start X-correlation... INFO @ Sat, 03 Apr 2021 08:21:28: end of X-cor INFO @ Sat, 03 Apr 2021 08:21:28: #2 finished! INFO @ Sat, 03 Apr 2021 08:21:28: #2 predicted fragment length is 97 bps INFO @ Sat, 03 Apr 2021 08:21:28: #2 alternative fragment length(s) may be 97 bps INFO @ Sat, 03 Apr 2021 08:21:28: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX7281006/SRX7281006.20_model.r WARNING @ Sat, 03 Apr 2021 08:21:28: #2 Since the d (97) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 08:21:28: #2 You may need to consider one of the other alternative d(s): 97 WARNING @ Sat, 03 Apr 2021 08:21:28: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 08:21:28: #3 Call peaks... INFO @ Sat, 03 Apr 2021 08:21:28: #3 Pre-compute pvalue-qvalue table... BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 08:21:39: #3 Call peaks for each chromosome... BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 08:21:45: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX7281006/SRX7281006.20_peaks.xls INFO @ Sat, 03 Apr 2021 08:21:45: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX7281006/SRX7281006.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 08:21:45: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX7281006/SRX7281006.20_summits.bed INFO @ Sat, 03 Apr 2021 08:21:45: Done! pass1 - making usageList (13 chroms): 1 millis pass2 - checking and writing primary data (6588 records, 4 fields): 10 millis CompletedMACS2peakCalling