Job ID = 12265657 SRX = SRX7277057 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 35704899 spots for SRR10597322/SRR10597322.sra Written 35704899 spots for SRR10597322/SRR10597322.sra fastq に変換しました。 bowtie でマッピング中... Your job 12266106 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:41:52 35704899 reads; of these: 35704899 (100.00%) were paired; of these: 1365277 (3.82%) aligned concordantly 0 times 12061857 (33.78%) aligned concordantly exactly 1 time 22277765 (62.39%) aligned concordantly >1 times ---- 1365277 pairs aligned concordantly 0 times; of these: 341560 (25.02%) aligned discordantly 1 time ---- 1023717 pairs aligned 0 times concordantly or discordantly; of these: 2047434 mates make up the pairs; of these: 1066539 (52.09%) aligned 0 times 389402 (19.02%) aligned exactly 1 time 591493 (28.89%) aligned >1 times 98.51% overall alignment rate Time searching: 00:41:53 Overall time: 00:41:53 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 18512543 / 34600082 = 0.5350 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:55:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7277057/SRX7277057.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7277057/SRX7277057.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7277057/SRX7277057.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7277057/SRX7277057.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:55:48: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:55:48: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:55:53: 1000000 INFO @ Sat, 03 Apr 2021 08:55:57: 2000000 INFO @ Sat, 03 Apr 2021 08:56:02: 3000000 INFO @ Sat, 03 Apr 2021 08:56:06: 4000000 INFO @ Sat, 03 Apr 2021 08:56:10: 5000000 INFO @ Sat, 03 Apr 2021 08:56:15: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:56:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7277057/SRX7277057.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7277057/SRX7277057.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7277057/SRX7277057.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7277057/SRX7277057.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:56:18: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:56:18: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:56:19: 7000000 INFO @ Sat, 03 Apr 2021 08:56:24: 1000000 INFO @ Sat, 03 Apr 2021 08:56:24: 8000000 INFO @ Sat, 03 Apr 2021 08:56:28: 9000000 INFO @ Sat, 03 Apr 2021 08:56:29: 2000000 INFO @ Sat, 03 Apr 2021 08:56:33: 10000000 INFO @ Sat, 03 Apr 2021 08:56:34: 3000000 INFO @ Sat, 03 Apr 2021 08:56:38: 11000000 INFO @ Sat, 03 Apr 2021 08:56:39: 4000000 INFO @ Sat, 03 Apr 2021 08:56:42: 12000000 INFO @ Sat, 03 Apr 2021 08:56:45: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:56:47: 13000000 INFO @ Sat, 03 Apr 2021 08:56:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7277057/SRX7277057.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7277057/SRX7277057.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7277057/SRX7277057.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7277057/SRX7277057.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:56:49: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:56:49: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:56:51: 6000000 INFO @ Sat, 03 Apr 2021 08:56:52: 14000000 INFO @ Sat, 03 Apr 2021 08:56:54: 1000000 INFO @ Sat, 03 Apr 2021 08:56:56: 7000000 INFO @ Sat, 03 Apr 2021 08:56:58: 15000000 INFO @ Sat, 03 Apr 2021 08:56:59: 2000000 INFO @ Sat, 03 Apr 2021 08:57:02: 8000000 INFO @ Sat, 03 Apr 2021 08:57:03: 16000000 INFO @ Sat, 03 Apr 2021 08:57:04: 3000000 INFO @ Sat, 03 Apr 2021 08:57:07: 9000000 INFO @ Sat, 03 Apr 2021 08:57:08: 17000000 INFO @ Sat, 03 Apr 2021 08:57:10: 4000000 INFO @ Sat, 03 Apr 2021 08:57:12: 10000000 INFO @ Sat, 03 Apr 2021 08:57:13: 18000000 INFO @ Sat, 03 Apr 2021 08:57:15: 5000000 INFO @ Sat, 03 Apr 2021 08:57:18: 11000000 INFO @ Sat, 03 Apr 2021 08:57:19: 19000000 INFO @ Sat, 03 Apr 2021 08:57:20: 6000000 INFO @ Sat, 03 Apr 2021 08:57:24: 12000000 INFO @ Sat, 03 Apr 2021 08:57:24: 20000000 INFO @ Sat, 03 Apr 2021 08:57:25: 7000000 INFO @ Sat, 03 Apr 2021 08:57:28: 21000000 INFO @ Sat, 03 Apr 2021 08:57:29: 13000000 INFO @ Sat, 03 Apr 2021 08:57:31: 8000000 INFO @ Sat, 03 Apr 2021 08:57:34: 22000000 INFO @ Sat, 03 Apr 2021 08:57:35: 14000000 INFO @ Sat, 03 Apr 2021 08:57:36: 9000000 INFO @ Sat, 03 Apr 2021 08:57:39: 23000000 INFO @ Sat, 03 Apr 2021 08:57:40: 15000000 INFO @ Sat, 03 Apr 2021 08:57:41: 10000000 INFO @ Sat, 03 Apr 2021 08:57:44: 24000000 INFO @ Sat, 03 Apr 2021 08:57:45: 16000000 INFO @ Sat, 03 Apr 2021 08:57:47: 11000000 INFO @ Sat, 03 Apr 2021 08:57:49: 25000000 INFO @ Sat, 03 Apr 2021 08:57:51: 17000000 INFO @ Sat, 03 Apr 2021 08:57:52: 12000000 INFO @ Sat, 03 Apr 2021 08:57:54: 26000000 INFO @ Sat, 03 Apr 2021 08:57:56: 18000000 INFO @ Sat, 03 Apr 2021 08:57:57: 13000000 INFO @ Sat, 03 Apr 2021 08:57:59: 27000000 INFO @ Sat, 03 Apr 2021 08:58:02: 19000000 INFO @ Sat, 03 Apr 2021 08:58:03: 14000000 INFO @ Sat, 03 Apr 2021 08:58:05: 28000000 INFO @ Sat, 03 Apr 2021 08:58:07: 20000000 INFO @ Sat, 03 Apr 2021 08:58:08: 15000000 INFO @ Sat, 03 Apr 2021 08:58:10: 29000000 INFO @ Sat, 03 Apr 2021 08:58:12: 21000000 INFO @ Sat, 03 Apr 2021 08:58:13: 16000000 INFO @ Sat, 03 Apr 2021 08:58:15: 30000000 INFO @ Sat, 03 Apr 2021 08:58:17: 22000000 INFO @ Sat, 03 Apr 2021 08:58:18: 17000000 INFO @ Sat, 03 Apr 2021 08:58:20: 31000000 INFO @ Sat, 03 Apr 2021 08:58:22: 23000000 INFO @ Sat, 03 Apr 2021 08:58:24: 18000000 INFO @ Sat, 03 Apr 2021 08:58:26: 32000000 INFO @ Sat, 03 Apr 2021 08:58:28: 24000000 INFO @ Sat, 03 Apr 2021 08:58:29: 19000000 INFO @ Sat, 03 Apr 2021 08:58:31: 33000000 INFO @ Sat, 03 Apr 2021 08:58:33: #1 tag size is determined as 36 bps INFO @ Sat, 03 Apr 2021 08:58:33: #1 tag size = 36 INFO @ Sat, 03 Apr 2021 08:58:33: #1 total tags in treatment: 15849633 INFO @ Sat, 03 Apr 2021 08:58:33: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:58:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:58:33: 25000000 INFO @ Sat, 03 Apr 2021 08:58:33: #1 tags after filtering in treatment: 11941028 INFO @ Sat, 03 Apr 2021 08:58:33: #1 Redundant rate of treatment: 0.25 INFO @ Sat, 03 Apr 2021 08:58:33: #1 finished! INFO @ Sat, 03 Apr 2021 08:58:33: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:58:33: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:58:34: 20000000 INFO @ Sat, 03 Apr 2021 08:58:34: #2 number of paired peaks: 93 WARNING @ Sat, 03 Apr 2021 08:58:34: Too few paired peaks (93) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 08:58:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7277057/SRX7277057.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7277057/SRX7277057.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7277057/SRX7277057.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7277057/SRX7277057.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 08:58:38: 21000000 INFO @ Sat, 03 Apr 2021 08:58:39: 26000000 INFO @ Sat, 03 Apr 2021 08:58:43: 22000000 INFO @ Sat, 03 Apr 2021 08:58:44: 27000000 INFO @ Sat, 03 Apr 2021 08:58:47: 23000000 INFO @ Sat, 03 Apr 2021 08:58:50: 28000000 INFO @ Sat, 03 Apr 2021 08:58:52: 24000000 INFO @ Sat, 03 Apr 2021 08:58:55: 29000000 INFO @ Sat, 03 Apr 2021 08:58:56: 25000000 INFO @ Sat, 03 Apr 2021 08:59:01: 26000000 INFO @ Sat, 03 Apr 2021 08:59:01: 30000000 INFO @ Sat, 03 Apr 2021 08:59:06: 27000000 INFO @ Sat, 03 Apr 2021 08:59:07: 31000000 INFO @ Sat, 03 Apr 2021 08:59:11: 28000000 INFO @ Sat, 03 Apr 2021 08:59:12: 32000000 INFO @ Sat, 03 Apr 2021 08:59:15: 29000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 08:59:18: 33000000 INFO @ Sat, 03 Apr 2021 08:59:20: #1 tag size is determined as 36 bps INFO @ Sat, 03 Apr 2021 08:59:20: #1 tag size = 36 INFO @ Sat, 03 Apr 2021 08:59:20: #1 total tags in treatment: 15849633 INFO @ Sat, 03 Apr 2021 08:59:20: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:59:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:59:20: 30000000 INFO @ Sat, 03 Apr 2021 08:59:20: #1 tags after filtering in treatment: 11941028 INFO @ Sat, 03 Apr 2021 08:59:20: #1 Redundant rate of treatment: 0.25 INFO @ Sat, 03 Apr 2021 08:59:20: #1 finished! INFO @ Sat, 03 Apr 2021 08:59:20: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:59:20: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:59:21: #2 number of paired peaks: 93 WARNING @ Sat, 03 Apr 2021 08:59:21: Too few paired peaks (93) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 08:59:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7277057/SRX7277057.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7277057/SRX7277057.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7277057/SRX7277057.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7277057/SRX7277057.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 08:59:24: 31000000 INFO @ Sat, 03 Apr 2021 08:59:29: 32000000 INFO @ Sat, 03 Apr 2021 08:59:33: 33000000 INFO @ Sat, 03 Apr 2021 08:59:35: #1 tag size is determined as 36 bps INFO @ Sat, 03 Apr 2021 08:59:35: #1 tag size = 36 INFO @ Sat, 03 Apr 2021 08:59:35: #1 total tags in treatment: 15849633 INFO @ Sat, 03 Apr 2021 08:59:35: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:59:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:59:35: #1 tags after filtering in treatment: 11941028 INFO @ Sat, 03 Apr 2021 08:59:35: #1 Redundant rate of treatment: 0.25 INFO @ Sat, 03 Apr 2021 08:59:35: #1 finished! INFO @ Sat, 03 Apr 2021 08:59:35: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:59:35: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:59:36: #2 number of paired peaks: 93 WARNING @ Sat, 03 Apr 2021 08:59:36: Too few paired peaks (93) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 08:59:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7277057/SRX7277057.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7277057/SRX7277057.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7277057/SRX7277057.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7277057/SRX7277057.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。