Job ID = 12265649 SRX = SRX7277051 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 37785092 spots for SRR10597316/SRR10597316.sra Written 37785092 spots for SRR10597316/SRR10597316.sra fastq に変換しました。 bowtie でマッピング中... Your job 12266188 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:47:47 37785092 reads; of these: 37785092 (100.00%) were paired; of these: 1599436 (4.23%) aligned concordantly 0 times 13763623 (36.43%) aligned concordantly exactly 1 time 22422033 (59.34%) aligned concordantly >1 times ---- 1599436 pairs aligned concordantly 0 times; of these: 445551 (27.86%) aligned discordantly 1 time ---- 1153885 pairs aligned 0 times concordantly or discordantly; of these: 2307770 mates make up the pairs; of these: 1130482 (48.99%) aligned 0 times 463719 (20.09%) aligned exactly 1 time 713569 (30.92%) aligned >1 times 98.50% overall alignment rate Time searching: 00:47:47 Overall time: 00:47:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 18287497 / 36554412 = 0.5003 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:59:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7277051/SRX7277051.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7277051/SRX7277051.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7277051/SRX7277051.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7277051/SRX7277051.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:59:39: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:59:39: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:59:44: 1000000 INFO @ Sat, 03 Apr 2021 08:59:48: 2000000 INFO @ Sat, 03 Apr 2021 08:59:52: 3000000 INFO @ Sat, 03 Apr 2021 08:59:57: 4000000 INFO @ Sat, 03 Apr 2021 09:00:01: 5000000 INFO @ Sat, 03 Apr 2021 09:00:06: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 09:00:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7277051/SRX7277051.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7277051/SRX7277051.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7277051/SRX7277051.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7277051/SRX7277051.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 09:00:10: #1 read tag files... INFO @ Sat, 03 Apr 2021 09:00:10: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 09:00:10: 7000000 INFO @ Sat, 03 Apr 2021 09:00:14: 1000000 INFO @ Sat, 03 Apr 2021 09:00:15: 8000000 INFO @ Sat, 03 Apr 2021 09:00:19: 2000000 INFO @ Sat, 03 Apr 2021 09:00:19: 9000000 INFO @ Sat, 03 Apr 2021 09:00:23: 3000000 INFO @ Sat, 03 Apr 2021 09:00:23: 10000000 INFO @ Sat, 03 Apr 2021 09:00:27: 4000000 INFO @ Sat, 03 Apr 2021 09:00:28: 11000000 INFO @ Sat, 03 Apr 2021 09:00:32: 5000000 INFO @ Sat, 03 Apr 2021 09:00:32: 12000000 INFO @ Sat, 03 Apr 2021 09:00:36: 6000000 INFO @ Sat, 03 Apr 2021 09:00:37: 13000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 09:00:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7277051/SRX7277051.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7277051/SRX7277051.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7277051/SRX7277051.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7277051/SRX7277051.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 09:00:39: #1 read tag files... INFO @ Sat, 03 Apr 2021 09:00:39: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 09:00:41: 7000000 INFO @ Sat, 03 Apr 2021 09:00:41: 14000000 INFO @ Sat, 03 Apr 2021 09:00:44: 1000000 INFO @ Sat, 03 Apr 2021 09:00:45: 8000000 INFO @ Sat, 03 Apr 2021 09:00:45: 15000000 INFO @ Sat, 03 Apr 2021 09:00:48: 2000000 INFO @ Sat, 03 Apr 2021 09:00:49: 9000000 INFO @ Sat, 03 Apr 2021 09:00:50: 16000000 INFO @ Sat, 03 Apr 2021 09:00:53: 3000000 INFO @ Sat, 03 Apr 2021 09:00:54: 10000000 INFO @ Sat, 03 Apr 2021 09:00:54: 17000000 INFO @ Sat, 03 Apr 2021 09:00:57: 4000000 INFO @ Sat, 03 Apr 2021 09:00:58: 11000000 INFO @ Sat, 03 Apr 2021 09:00:59: 18000000 INFO @ Sat, 03 Apr 2021 09:01:01: 5000000 INFO @ Sat, 03 Apr 2021 09:01:03: 12000000 INFO @ Sat, 03 Apr 2021 09:01:03: 19000000 INFO @ Sat, 03 Apr 2021 09:01:06: 6000000 INFO @ Sat, 03 Apr 2021 09:01:07: 13000000 INFO @ Sat, 03 Apr 2021 09:01:07: 20000000 INFO @ Sat, 03 Apr 2021 09:01:10: 7000000 INFO @ Sat, 03 Apr 2021 09:01:12: 14000000 INFO @ Sat, 03 Apr 2021 09:01:12: 21000000 INFO @ Sat, 03 Apr 2021 09:01:15: 8000000 INFO @ Sat, 03 Apr 2021 09:01:16: 15000000 INFO @ Sat, 03 Apr 2021 09:01:16: 22000000 INFO @ Sat, 03 Apr 2021 09:01:19: 9000000 INFO @ Sat, 03 Apr 2021 09:01:20: 16000000 INFO @ Sat, 03 Apr 2021 09:01:21: 23000000 INFO @ Sat, 03 Apr 2021 09:01:24: 10000000 INFO @ Sat, 03 Apr 2021 09:01:25: 24000000 INFO @ Sat, 03 Apr 2021 09:01:25: 17000000 INFO @ Sat, 03 Apr 2021 09:01:28: 11000000 INFO @ Sat, 03 Apr 2021 09:01:29: 25000000 INFO @ Sat, 03 Apr 2021 09:01:29: 18000000 INFO @ Sat, 03 Apr 2021 09:01:32: 12000000 INFO @ Sat, 03 Apr 2021 09:01:33: 26000000 INFO @ Sat, 03 Apr 2021 09:01:34: 19000000 INFO @ Sat, 03 Apr 2021 09:01:37: 13000000 INFO @ Sat, 03 Apr 2021 09:01:38: 27000000 INFO @ Sat, 03 Apr 2021 09:01:38: 20000000 INFO @ Sat, 03 Apr 2021 09:01:41: 14000000 INFO @ Sat, 03 Apr 2021 09:01:42: 28000000 INFO @ Sat, 03 Apr 2021 09:01:43: 21000000 INFO @ Sat, 03 Apr 2021 09:01:46: 15000000 INFO @ Sat, 03 Apr 2021 09:01:46: 29000000 INFO @ Sat, 03 Apr 2021 09:01:47: 22000000 INFO @ Sat, 03 Apr 2021 09:01:50: 16000000 INFO @ Sat, 03 Apr 2021 09:01:51: 30000000 INFO @ Sat, 03 Apr 2021 09:01:51: 23000000 INFO @ Sat, 03 Apr 2021 09:01:55: 17000000 INFO @ Sat, 03 Apr 2021 09:01:55: 31000000 INFO @ Sat, 03 Apr 2021 09:01:56: 24000000 INFO @ Sat, 03 Apr 2021 09:01:59: 18000000 INFO @ Sat, 03 Apr 2021 09:02:00: 32000000 INFO @ Sat, 03 Apr 2021 09:02:00: 25000000 INFO @ Sat, 03 Apr 2021 09:02:04: 19000000 INFO @ Sat, 03 Apr 2021 09:02:04: 33000000 INFO @ Sat, 03 Apr 2021 09:02:04: 26000000 INFO @ Sat, 03 Apr 2021 09:02:08: 20000000 INFO @ Sat, 03 Apr 2021 09:02:09: 34000000 INFO @ Sat, 03 Apr 2021 09:02:09: 27000000 INFO @ Sat, 03 Apr 2021 09:02:13: 21000000 INFO @ Sat, 03 Apr 2021 09:02:13: 35000000 INFO @ Sat, 03 Apr 2021 09:02:13: 28000000 INFO @ Sat, 03 Apr 2021 09:02:17: 22000000 INFO @ Sat, 03 Apr 2021 09:02:17: 36000000 INFO @ Sat, 03 Apr 2021 09:02:17: 29000000 INFO @ Sat, 03 Apr 2021 09:02:21: 23000000 INFO @ Sat, 03 Apr 2021 09:02:22: 37000000 INFO @ Sat, 03 Apr 2021 09:02:22: 30000000 INFO @ Sat, 03 Apr 2021 09:02:26: 24000000 INFO @ Sat, 03 Apr 2021 09:02:26: #1 tag size is determined as 36 bps INFO @ Sat, 03 Apr 2021 09:02:26: #1 tag size = 36 INFO @ Sat, 03 Apr 2021 09:02:26: #1 total tags in treatment: 17927850 INFO @ Sat, 03 Apr 2021 09:02:26: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 09:02:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 09:02:26: #1 tags after filtering in treatment: 13638947 INFO @ Sat, 03 Apr 2021 09:02:26: #1 Redundant rate of treatment: 0.24 INFO @ Sat, 03 Apr 2021 09:02:26: #1 finished! INFO @ Sat, 03 Apr 2021 09:02:26: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 09:02:26: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 09:02:26: 31000000 INFO @ Sat, 03 Apr 2021 09:02:27: #2 number of paired peaks: 79 WARNING @ Sat, 03 Apr 2021 09:02:27: Too few paired peaks (79) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 09:02:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7277051/SRX7277051.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7277051/SRX7277051.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7277051/SRX7277051.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7277051/SRX7277051.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 09:02:30: 25000000 INFO @ Sat, 03 Apr 2021 09:02:31: 32000000 INFO @ Sat, 03 Apr 2021 09:02:34: 26000000 INFO @ Sat, 03 Apr 2021 09:02:35: 33000000 INFO @ Sat, 03 Apr 2021 09:02:39: 27000000 INFO @ Sat, 03 Apr 2021 09:02:40: 34000000 INFO @ Sat, 03 Apr 2021 09:02:43: 28000000 INFO @ Sat, 03 Apr 2021 09:02:44: 35000000 INFO @ Sat, 03 Apr 2021 09:02:47: 29000000 INFO @ Sat, 03 Apr 2021 09:02:49: 36000000 INFO @ Sat, 03 Apr 2021 09:02:52: 30000000 INFO @ Sat, 03 Apr 2021 09:02:54: 37000000 INFO @ Sat, 03 Apr 2021 09:02:56: 31000000 INFO @ Sat, 03 Apr 2021 09:02:58: #1 tag size is determined as 36 bps INFO @ Sat, 03 Apr 2021 09:02:58: #1 tag size = 36 INFO @ Sat, 03 Apr 2021 09:02:58: #1 total tags in treatment: 17927850 INFO @ Sat, 03 Apr 2021 09:02:58: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 09:02:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 09:02:58: #1 tags after filtering in treatment: 13638947 INFO @ Sat, 03 Apr 2021 09:02:58: #1 Redundant rate of treatment: 0.24 INFO @ Sat, 03 Apr 2021 09:02:58: #1 finished! INFO @ Sat, 03 Apr 2021 09:02:58: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 09:02:58: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 09:02:59: #2 number of paired peaks: 79 WARNING @ Sat, 03 Apr 2021 09:02:59: Too few paired peaks (79) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 09:02:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7277051/SRX7277051.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7277051/SRX7277051.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7277051/SRX7277051.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7277051/SRX7277051.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 09:03:01: 32000000 INFO @ Sat, 03 Apr 2021 09:03:05: 33000000 INFO @ Sat, 03 Apr 2021 09:03:09: 34000000 INFO @ Sat, 03 Apr 2021 09:03:14: 35000000 INFO @ Sat, 03 Apr 2021 09:03:18: 36000000 INFO @ Sat, 03 Apr 2021 09:03:22: 37000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 09:03:26: #1 tag size is determined as 36 bps INFO @ Sat, 03 Apr 2021 09:03:26: #1 tag size = 36 INFO @ Sat, 03 Apr 2021 09:03:26: #1 total tags in treatment: 17927850 INFO @ Sat, 03 Apr 2021 09:03:26: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 09:03:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 09:03:27: #1 tags after filtering in treatment: 13638947 INFO @ Sat, 03 Apr 2021 09:03:27: #1 Redundant rate of treatment: 0.24 INFO @ Sat, 03 Apr 2021 09:03:27: #1 finished! INFO @ Sat, 03 Apr 2021 09:03:27: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 09:03:27: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 09:03:28: #2 number of paired peaks: 79 WARNING @ Sat, 03 Apr 2021 09:03:28: Too few paired peaks (79) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 09:03:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7277051/SRX7277051.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7277051/SRX7277051.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7277051/SRX7277051.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7277051/SRX7277051.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。