Job ID = 12265642 SRX = SRX7277047 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 35301950 spots for SRR10597312/SRR10597312.sra Written 35301950 spots for SRR10597312/SRR10597312.sra fastq に変換しました。 bowtie でマッピング中... Your job 12266232 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:50:42 35301950 reads; of these: 35301950 (100.00%) were paired; of these: 1772086 (5.02%) aligned concordantly 0 times 14737686 (41.75%) aligned concordantly exactly 1 time 18792178 (53.23%) aligned concordantly >1 times ---- 1772086 pairs aligned concordantly 0 times; of these: 519679 (29.33%) aligned discordantly 1 time ---- 1252407 pairs aligned 0 times concordantly or discordantly; of these: 2504814 mates make up the pairs; of these: 1249611 (49.89%) aligned 0 times 481174 (19.21%) aligned exactly 1 time 774029 (30.90%) aligned >1 times 98.23% overall alignment rate Time searching: 00:50:42 Overall time: 00:50:42 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 15887021 / 33935096 = 0.4682 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 09:02:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7277047/SRX7277047.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7277047/SRX7277047.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7277047/SRX7277047.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7277047/SRX7277047.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 09:02:32: #1 read tag files... INFO @ Sat, 03 Apr 2021 09:02:32: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 09:02:37: 1000000 INFO @ Sat, 03 Apr 2021 09:02:43: 2000000 INFO @ Sat, 03 Apr 2021 09:02:48: 3000000 INFO @ Sat, 03 Apr 2021 09:02:54: 4000000 INFO @ Sat, 03 Apr 2021 09:02:59: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 09:03:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7277047/SRX7277047.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7277047/SRX7277047.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7277047/SRX7277047.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7277047/SRX7277047.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 09:03:02: #1 read tag files... INFO @ Sat, 03 Apr 2021 09:03:02: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 09:03:04: 6000000 INFO @ Sat, 03 Apr 2021 09:03:08: 1000000 INFO @ Sat, 03 Apr 2021 09:03:09: 7000000 INFO @ Sat, 03 Apr 2021 09:03:14: 2000000 INFO @ Sat, 03 Apr 2021 09:03:15: 8000000 INFO @ Sat, 03 Apr 2021 09:03:19: 3000000 INFO @ Sat, 03 Apr 2021 09:03:20: 9000000 INFO @ Sat, 03 Apr 2021 09:03:25: 4000000 INFO @ Sat, 03 Apr 2021 09:03:26: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 09:03:30: 5000000 INFO @ Sat, 03 Apr 2021 09:03:31: 11000000 INFO @ Sat, 03 Apr 2021 09:03:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7277047/SRX7277047.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7277047/SRX7277047.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7277047/SRX7277047.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7277047/SRX7277047.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 09:03:32: #1 read tag files... INFO @ Sat, 03 Apr 2021 09:03:32: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 09:03:36: 12000000 INFO @ Sat, 03 Apr 2021 09:03:36: 6000000 INFO @ Sat, 03 Apr 2021 09:03:39: 1000000 INFO @ Sat, 03 Apr 2021 09:03:42: 13000000 INFO @ Sat, 03 Apr 2021 09:03:43: 7000000 INFO @ Sat, 03 Apr 2021 09:03:45: 2000000 INFO @ Sat, 03 Apr 2021 09:03:47: 14000000 INFO @ Sat, 03 Apr 2021 09:03:49: 8000000 INFO @ Sat, 03 Apr 2021 09:03:52: 3000000 INFO @ Sat, 03 Apr 2021 09:03:52: 15000000 INFO @ Sat, 03 Apr 2021 09:03:55: 9000000 INFO @ Sat, 03 Apr 2021 09:03:58: 16000000 INFO @ Sat, 03 Apr 2021 09:03:58: 4000000 INFO @ Sat, 03 Apr 2021 09:04:01: 10000000 INFO @ Sat, 03 Apr 2021 09:04:04: 17000000 INFO @ Sat, 03 Apr 2021 09:04:04: 5000000 INFO @ Sat, 03 Apr 2021 09:04:07: 11000000 INFO @ Sat, 03 Apr 2021 09:04:09: 18000000 INFO @ Sat, 03 Apr 2021 09:04:10: 6000000 INFO @ Sat, 03 Apr 2021 09:04:14: 12000000 INFO @ Sat, 03 Apr 2021 09:04:14: 19000000 INFO @ Sat, 03 Apr 2021 09:04:16: 7000000 INFO @ Sat, 03 Apr 2021 09:04:20: 13000000 INFO @ Sat, 03 Apr 2021 09:04:20: 20000000 INFO @ Sat, 03 Apr 2021 09:04:23: 8000000 INFO @ Sat, 03 Apr 2021 09:04:25: 21000000 INFO @ Sat, 03 Apr 2021 09:04:26: 14000000 INFO @ Sat, 03 Apr 2021 09:04:29: 9000000 INFO @ Sat, 03 Apr 2021 09:04:32: 22000000 INFO @ Sat, 03 Apr 2021 09:04:33: 15000000 INFO @ Sat, 03 Apr 2021 09:04:36: 10000000 INFO @ Sat, 03 Apr 2021 09:04:37: 23000000 INFO @ Sat, 03 Apr 2021 09:04:40: 16000000 INFO @ Sat, 03 Apr 2021 09:04:42: 24000000 INFO @ Sat, 03 Apr 2021 09:04:42: 11000000 INFO @ Sat, 03 Apr 2021 09:04:46: 17000000 INFO @ Sat, 03 Apr 2021 09:04:47: 25000000 INFO @ Sat, 03 Apr 2021 09:04:49: 12000000 INFO @ Sat, 03 Apr 2021 09:04:52: 26000000 INFO @ Sat, 03 Apr 2021 09:04:53: 18000000 INFO @ Sat, 03 Apr 2021 09:04:56: 13000000 INFO @ Sat, 03 Apr 2021 09:04:58: 27000000 INFO @ Sat, 03 Apr 2021 09:04:59: 19000000 INFO @ Sat, 03 Apr 2021 09:05:02: 14000000 INFO @ Sat, 03 Apr 2021 09:05:03: 28000000 INFO @ Sat, 03 Apr 2021 09:05:06: 20000000 INFO @ Sat, 03 Apr 2021 09:05:08: 29000000 INFO @ Sat, 03 Apr 2021 09:05:08: 15000000 INFO @ Sat, 03 Apr 2021 09:05:12: 21000000 INFO @ Sat, 03 Apr 2021 09:05:14: 30000000 INFO @ Sat, 03 Apr 2021 09:05:15: 16000000 INFO @ Sat, 03 Apr 2021 09:05:19: 22000000 INFO @ Sat, 03 Apr 2021 09:05:19: 31000000 INFO @ Sat, 03 Apr 2021 09:05:21: 17000000 INFO @ Sat, 03 Apr 2021 09:05:25: 32000000 INFO @ Sat, 03 Apr 2021 09:05:25: 23000000 INFO @ Sat, 03 Apr 2021 09:05:27: 18000000 INFO @ Sat, 03 Apr 2021 09:05:30: 33000000 INFO @ Sat, 03 Apr 2021 09:05:31: 24000000 INFO @ Sat, 03 Apr 2021 09:05:34: 19000000 INFO @ Sat, 03 Apr 2021 09:05:35: 34000000 INFO @ Sat, 03 Apr 2021 09:05:37: 25000000 INFO @ Sat, 03 Apr 2021 09:05:41: 20000000 INFO @ Sat, 03 Apr 2021 09:05:42: 35000000 INFO @ Sat, 03 Apr 2021 09:05:44: 26000000 INFO @ Sat, 03 Apr 2021 09:05:47: 36000000 INFO @ Sat, 03 Apr 2021 09:05:47: 21000000 INFO @ Sat, 03 Apr 2021 09:05:51: 27000000 INFO @ Sat, 03 Apr 2021 09:05:52: 37000000 INFO @ Sat, 03 Apr 2021 09:05:54: 22000000 INFO @ Sat, 03 Apr 2021 09:05:56: #1 tag size is determined as 36 bps INFO @ Sat, 03 Apr 2021 09:05:56: #1 tag size = 36 INFO @ Sat, 03 Apr 2021 09:05:56: #1 total tags in treatment: 17701446 INFO @ Sat, 03 Apr 2021 09:05:56: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 09:05:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 09:05:56: 28000000 INFO @ Sat, 03 Apr 2021 09:05:57: #1 tags after filtering in treatment: 13577151 INFO @ Sat, 03 Apr 2021 09:05:57: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 03 Apr 2021 09:05:57: #1 finished! INFO @ Sat, 03 Apr 2021 09:05:57: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 09:05:57: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 09:05:57: #2 number of paired peaks: 80 WARNING @ Sat, 03 Apr 2021 09:05:57: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 09:05:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7277047/SRX7277047.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7277047/SRX7277047.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7277047/SRX7277047.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7277047/SRX7277047.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 09:06:00: 23000000 INFO @ Sat, 03 Apr 2021 09:06:02: 29000000 INFO @ Sat, 03 Apr 2021 09:06:05: 24000000 INFO @ Sat, 03 Apr 2021 09:06:08: 30000000 INFO @ Sat, 03 Apr 2021 09:06:11: 25000000 INFO @ Sat, 03 Apr 2021 09:06:14: 31000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 09:06:17: 26000000 INFO @ Sat, 03 Apr 2021 09:06:20: 32000000 INFO @ Sat, 03 Apr 2021 09:06:22: 27000000 INFO @ Sat, 03 Apr 2021 09:06:27: 33000000 INFO @ Sat, 03 Apr 2021 09:06:29: 28000000 INFO @ Sat, 03 Apr 2021 09:06:33: 34000000 INFO @ Sat, 03 Apr 2021 09:06:35: 29000000 INFO @ Sat, 03 Apr 2021 09:06:39: 35000000 INFO @ Sat, 03 Apr 2021 09:06:41: 30000000 INFO @ Sat, 03 Apr 2021 09:06:45: 36000000 INFO @ Sat, 03 Apr 2021 09:06:46: 31000000 INFO @ Sat, 03 Apr 2021 09:06:51: 37000000 INFO @ Sat, 03 Apr 2021 09:06:52: 32000000 INFO @ Sat, 03 Apr 2021 09:06:55: #1 tag size is determined as 36 bps INFO @ Sat, 03 Apr 2021 09:06:55: #1 tag size = 36 INFO @ Sat, 03 Apr 2021 09:06:55: #1 total tags in treatment: 17701446 INFO @ Sat, 03 Apr 2021 09:06:55: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 09:06:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 09:06:55: #1 tags after filtering in treatment: 13577151 INFO @ Sat, 03 Apr 2021 09:06:55: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 03 Apr 2021 09:06:55: #1 finished! INFO @ Sat, 03 Apr 2021 09:06:55: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 09:06:55: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 09:06:56: #2 number of paired peaks: 80 WARNING @ Sat, 03 Apr 2021 09:06:56: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 09:06:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7277047/SRX7277047.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7277047/SRX7277047.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7277047/SRX7277047.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7277047/SRX7277047.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 09:06:58: 33000000 INFO @ Sat, 03 Apr 2021 09:07:03: 34000000 INFO @ Sat, 03 Apr 2021 09:07:08: 35000000 INFO @ Sat, 03 Apr 2021 09:07:14: 36000000 INFO @ Sat, 03 Apr 2021 09:07:20: 37000000 INFO @ Sat, 03 Apr 2021 09:07:23: #1 tag size is determined as 36 bps INFO @ Sat, 03 Apr 2021 09:07:23: #1 tag size = 36 INFO @ Sat, 03 Apr 2021 09:07:23: #1 total tags in treatment: 17701446 INFO @ Sat, 03 Apr 2021 09:07:23: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 09:07:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 09:07:23: #1 tags after filtering in treatment: 13577151 INFO @ Sat, 03 Apr 2021 09:07:23: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 03 Apr 2021 09:07:23: #1 finished! INFO @ Sat, 03 Apr 2021 09:07:23: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 09:07:23: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 09:07:24: #2 number of paired peaks: 80 WARNING @ Sat, 03 Apr 2021 09:07:24: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 09:07:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7277047/SRX7277047.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7277047/SRX7277047.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7277047/SRX7277047.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7277047/SRX7277047.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。