Job ID = 12265641 SRX = SRX7277046 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 30014825 spots for SRR10597311/SRR10597311.sra Written 30014825 spots for SRR10597311/SRR10597311.sra fastq に変換しました。 bowtie でマッピング中... Your job 12266010 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:38:53 30014825 reads; of these: 30014825 (100.00%) were paired; of these: 1807417 (6.02%) aligned concordantly 0 times 14124011 (47.06%) aligned concordantly exactly 1 time 14083397 (46.92%) aligned concordantly >1 times ---- 1807417 pairs aligned concordantly 0 times; of these: 529814 (29.31%) aligned discordantly 1 time ---- 1277603 pairs aligned 0 times concordantly or discordantly; of these: 2555206 mates make up the pairs; of these: 1433143 (56.09%) aligned 0 times 468707 (18.34%) aligned exactly 1 time 653356 (25.57%) aligned >1 times 97.61% overall alignment rate Time searching: 00:38:53 Overall time: 00:38:53 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 9765899 / 28607420 = 0.3414 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:46:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7277046/SRX7277046.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7277046/SRX7277046.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7277046/SRX7277046.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7277046/SRX7277046.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:46:03: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:46:03: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:46:08: 1000000 INFO @ Sat, 03 Apr 2021 08:46:12: 2000000 INFO @ Sat, 03 Apr 2021 08:46:16: 3000000 INFO @ Sat, 03 Apr 2021 08:46:20: 4000000 INFO @ Sat, 03 Apr 2021 08:46:24: 5000000 INFO @ Sat, 03 Apr 2021 08:46:29: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:46:33: 7000000 INFO @ Sat, 03 Apr 2021 08:46:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7277046/SRX7277046.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7277046/SRX7277046.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7277046/SRX7277046.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7277046/SRX7277046.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:46:33: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:46:33: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:46:37: 8000000 INFO @ Sat, 03 Apr 2021 08:46:38: 1000000 INFO @ Sat, 03 Apr 2021 08:46:41: 9000000 INFO @ Sat, 03 Apr 2021 08:46:42: 2000000 INFO @ Sat, 03 Apr 2021 08:46:46: 10000000 INFO @ Sat, 03 Apr 2021 08:46:46: 3000000 INFO @ Sat, 03 Apr 2021 08:46:50: 11000000 INFO @ Sat, 03 Apr 2021 08:46:50: 4000000 INFO @ Sat, 03 Apr 2021 08:46:54: 12000000 INFO @ Sat, 03 Apr 2021 08:46:54: 5000000 INFO @ Sat, 03 Apr 2021 08:46:58: 13000000 INFO @ Sat, 03 Apr 2021 08:46:58: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:47:02: 14000000 INFO @ Sat, 03 Apr 2021 08:47:03: 7000000 INFO @ Sat, 03 Apr 2021 08:47:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7277046/SRX7277046.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7277046/SRX7277046.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7277046/SRX7277046.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7277046/SRX7277046.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:47:04: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:47:04: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:47:06: 15000000 INFO @ Sat, 03 Apr 2021 08:47:07: 8000000 INFO @ Sat, 03 Apr 2021 08:47:08: 1000000 INFO @ Sat, 03 Apr 2021 08:47:10: 16000000 INFO @ Sat, 03 Apr 2021 08:47:11: 9000000 INFO @ Sat, 03 Apr 2021 08:47:13: 2000000 INFO @ Sat, 03 Apr 2021 08:47:15: 17000000 INFO @ Sat, 03 Apr 2021 08:47:15: 10000000 INFO @ Sat, 03 Apr 2021 08:47:18: 3000000 INFO @ Sat, 03 Apr 2021 08:47:19: 18000000 INFO @ Sat, 03 Apr 2021 08:47:19: 11000000 INFO @ Sat, 03 Apr 2021 08:47:23: 19000000 INFO @ Sat, 03 Apr 2021 08:47:23: 12000000 INFO @ Sat, 03 Apr 2021 08:47:23: 4000000 INFO @ Sat, 03 Apr 2021 08:47:27: 20000000 INFO @ Sat, 03 Apr 2021 08:47:27: 13000000 INFO @ Sat, 03 Apr 2021 08:47:28: 5000000 INFO @ Sat, 03 Apr 2021 08:47:31: 21000000 INFO @ Sat, 03 Apr 2021 08:47:31: 14000000 INFO @ Sat, 03 Apr 2021 08:47:33: 6000000 INFO @ Sat, 03 Apr 2021 08:47:35: 22000000 INFO @ Sat, 03 Apr 2021 08:47:35: 15000000 INFO @ Sat, 03 Apr 2021 08:47:38: 7000000 INFO @ Sat, 03 Apr 2021 08:47:39: 23000000 INFO @ Sat, 03 Apr 2021 08:47:39: 16000000 INFO @ Sat, 03 Apr 2021 08:47:43: 8000000 INFO @ Sat, 03 Apr 2021 08:47:43: 17000000 INFO @ Sat, 03 Apr 2021 08:47:44: 24000000 INFO @ Sat, 03 Apr 2021 08:47:48: 18000000 INFO @ Sat, 03 Apr 2021 08:47:48: 25000000 INFO @ Sat, 03 Apr 2021 08:47:48: 9000000 INFO @ Sat, 03 Apr 2021 08:47:52: 19000000 INFO @ Sat, 03 Apr 2021 08:47:52: 26000000 INFO @ Sat, 03 Apr 2021 08:47:53: 10000000 INFO @ Sat, 03 Apr 2021 08:47:56: 20000000 INFO @ Sat, 03 Apr 2021 08:47:57: 27000000 INFO @ Sat, 03 Apr 2021 08:47:58: 11000000 INFO @ Sat, 03 Apr 2021 08:48:00: 21000000 INFO @ Sat, 03 Apr 2021 08:48:01: 28000000 INFO @ Sat, 03 Apr 2021 08:48:03: 12000000 INFO @ Sat, 03 Apr 2021 08:48:04: 22000000 INFO @ Sat, 03 Apr 2021 08:48:06: 29000000 INFO @ Sat, 03 Apr 2021 08:48:07: 13000000 INFO @ Sat, 03 Apr 2021 08:48:08: 23000000 INFO @ Sat, 03 Apr 2021 08:48:11: 30000000 INFO @ Sat, 03 Apr 2021 08:48:12: 24000000 INFO @ Sat, 03 Apr 2021 08:48:12: 14000000 INFO @ Sat, 03 Apr 2021 08:48:15: 31000000 INFO @ Sat, 03 Apr 2021 08:48:16: 25000000 INFO @ Sat, 03 Apr 2021 08:48:17: 15000000 INFO @ Sat, 03 Apr 2021 08:48:20: 32000000 INFO @ Sat, 03 Apr 2021 08:48:20: 26000000 INFO @ Sat, 03 Apr 2021 08:48:22: 16000000 INFO @ Sat, 03 Apr 2021 08:48:24: 27000000 INFO @ Sat, 03 Apr 2021 08:48:24: 33000000 INFO @ Sat, 03 Apr 2021 08:48:27: 17000000 INFO @ Sat, 03 Apr 2021 08:48:28: 28000000 INFO @ Sat, 03 Apr 2021 08:48:29: 34000000 INFO @ Sat, 03 Apr 2021 08:48:32: 18000000 INFO @ Sat, 03 Apr 2021 08:48:32: 29000000 INFO @ Sat, 03 Apr 2021 08:48:34: 35000000 INFO @ Sat, 03 Apr 2021 08:48:36: 30000000 INFO @ Sat, 03 Apr 2021 08:48:36: 19000000 INFO @ Sat, 03 Apr 2021 08:48:38: 36000000 INFO @ Sat, 03 Apr 2021 08:48:40: 31000000 INFO @ Sat, 03 Apr 2021 08:48:41: 20000000 INFO @ Sat, 03 Apr 2021 08:48:43: 37000000 INFO @ Sat, 03 Apr 2021 08:48:44: 32000000 INFO @ Sat, 03 Apr 2021 08:48:46: 21000000 INFO @ Sat, 03 Apr 2021 08:48:47: 38000000 INFO @ Sat, 03 Apr 2021 08:48:48: 33000000 INFO @ Sat, 03 Apr 2021 08:48:50: 22000000 INFO @ Sat, 03 Apr 2021 08:48:52: 34000000 INFO @ Sat, 03 Apr 2021 08:48:52: 39000000 INFO @ Sat, 03 Apr 2021 08:48:53: #1 tag size is determined as 36 bps INFO @ Sat, 03 Apr 2021 08:48:53: #1 tag size = 36 INFO @ Sat, 03 Apr 2021 08:48:53: #1 total tags in treatment: 18477810 INFO @ Sat, 03 Apr 2021 08:48:53: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:48:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:48:53: #1 tags after filtering in treatment: 14677196 INFO @ Sat, 03 Apr 2021 08:48:53: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 03 Apr 2021 08:48:53: #1 finished! INFO @ Sat, 03 Apr 2021 08:48:53: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:48:53: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:48:54: #2 number of paired peaks: 77 WARNING @ Sat, 03 Apr 2021 08:48:54: Too few paired peaks (77) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 08:48:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7277046/SRX7277046.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7277046/SRX7277046.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7277046/SRX7277046.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7277046/SRX7277046.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 08:48:55: 23000000 INFO @ Sat, 03 Apr 2021 08:48:56: 35000000 INFO @ Sat, 03 Apr 2021 08:48:59: 24000000 INFO @ Sat, 03 Apr 2021 08:49:00: 36000000 INFO @ Sat, 03 Apr 2021 08:49:04: 25000000 INFO @ Sat, 03 Apr 2021 08:49:04: 37000000 INFO @ Sat, 03 Apr 2021 08:49:08: 26000000 INFO @ Sat, 03 Apr 2021 08:49:08: 38000000 INFO @ Sat, 03 Apr 2021 08:49:12: 39000000 INFO @ Sat, 03 Apr 2021 08:49:12: #1 tag size is determined as 36 bps INFO @ Sat, 03 Apr 2021 08:49:12: #1 tag size = 36 INFO @ Sat, 03 Apr 2021 08:49:12: #1 total tags in treatment: 18477810 INFO @ Sat, 03 Apr 2021 08:49:12: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:49:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:49:12: 27000000 INFO @ Sat, 03 Apr 2021 08:49:13: #1 tags after filtering in treatment: 14677196 INFO @ Sat, 03 Apr 2021 08:49:13: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 03 Apr 2021 08:49:13: #1 finished! INFO @ Sat, 03 Apr 2021 08:49:13: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:49:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:49:14: #2 number of paired peaks: 77 WARNING @ Sat, 03 Apr 2021 08:49:14: Too few paired peaks (77) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 08:49:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7277046/SRX7277046.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7277046/SRX7277046.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7277046/SRX7277046.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7277046/SRX7277046.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 08:49:17: 28000000 INFO @ Sat, 03 Apr 2021 08:49:21: 29000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 08:49:26: 30000000 INFO @ Sat, 03 Apr 2021 08:49:30: 31000000 INFO @ Sat, 03 Apr 2021 08:49:35: 32000000 INFO @ Sat, 03 Apr 2021 08:49:40: 33000000 INFO @ Sat, 03 Apr 2021 08:49:45: 34000000 INFO @ Sat, 03 Apr 2021 08:49:49: 35000000 INFO @ Sat, 03 Apr 2021 08:49:54: 36000000 INFO @ Sat, 03 Apr 2021 08:49:58: 37000000 INFO @ Sat, 03 Apr 2021 08:50:03: 38000000 INFO @ Sat, 03 Apr 2021 08:50:07: 39000000 INFO @ Sat, 03 Apr 2021 08:50:08: #1 tag size is determined as 36 bps INFO @ Sat, 03 Apr 2021 08:50:08: #1 tag size = 36 INFO @ Sat, 03 Apr 2021 08:50:08: #1 total tags in treatment: 18477810 INFO @ Sat, 03 Apr 2021 08:50:08: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:50:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:50:08: #1 tags after filtering in treatment: 14677196 INFO @ Sat, 03 Apr 2021 08:50:08: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 03 Apr 2021 08:50:08: #1 finished! INFO @ Sat, 03 Apr 2021 08:50:08: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:50:08: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:50:09: #2 number of paired peaks: 77 WARNING @ Sat, 03 Apr 2021 08:50:09: Too few paired peaks (77) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 08:50:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7277046/SRX7277046.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7277046/SRX7277046.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7277046/SRX7277046.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7277046/SRX7277046.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。