Job ID = 12265600 SRX = SRX7277033 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 28931849 spots for SRR10597298/SRR10597298.sra Written 28931849 spots for SRR10597298/SRR10597298.sra fastq に変換しました。 bowtie でマッピング中... Your job 12266035 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:49:34 28931849 reads; of these: 28931849 (100.00%) were paired; of these: 11750788 (40.62%) aligned concordantly 0 times 11967532 (41.36%) aligned concordantly exactly 1 time 5213529 (18.02%) aligned concordantly >1 times ---- 11750788 pairs aligned concordantly 0 times; of these: 2804582 (23.87%) aligned discordantly 1 time ---- 8946206 pairs aligned 0 times concordantly or discordantly; of these: 17892412 mates make up the pairs; of these: 14638786 (81.82%) aligned 0 times 830457 (4.64%) aligned exactly 1 time 2423169 (13.54%) aligned >1 times 74.70% overall alignment rate Time searching: 00:49:34 Overall time: 00:49:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 2869921 / 19831159 = 0.1447 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:54:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7277033/SRX7277033.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7277033/SRX7277033.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7277033/SRX7277033.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7277033/SRX7277033.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:54:49: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:54:49: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:54:56: 1000000 INFO @ Sat, 03 Apr 2021 08:55:03: 2000000 INFO @ Sat, 03 Apr 2021 08:55:11: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:55:18: 4000000 INFO @ Sat, 03 Apr 2021 08:55:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7277033/SRX7277033.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7277033/SRX7277033.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7277033/SRX7277033.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7277033/SRX7277033.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:55:19: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:55:19: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:55:26: 5000000 INFO @ Sat, 03 Apr 2021 08:55:26: 1000000 INFO @ Sat, 03 Apr 2021 08:55:32: 2000000 INFO @ Sat, 03 Apr 2021 08:55:33: 6000000 INFO @ Sat, 03 Apr 2021 08:55:39: 3000000 INFO @ Sat, 03 Apr 2021 08:55:41: 7000000 INFO @ Sat, 03 Apr 2021 08:55:46: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:55:48: 8000000 INFO @ Sat, 03 Apr 2021 08:55:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7277033/SRX7277033.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7277033/SRX7277033.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7277033/SRX7277033.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7277033/SRX7277033.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:55:49: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:55:49: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:55:52: 5000000 INFO @ Sat, 03 Apr 2021 08:55:56: 9000000 INFO @ Sat, 03 Apr 2021 08:55:57: 1000000 INFO @ Sat, 03 Apr 2021 08:55:59: 6000000 INFO @ Sat, 03 Apr 2021 08:56:03: 10000000 INFO @ Sat, 03 Apr 2021 08:56:04: 2000000 INFO @ Sat, 03 Apr 2021 08:56:05: 7000000 INFO @ Sat, 03 Apr 2021 08:56:11: 11000000 INFO @ Sat, 03 Apr 2021 08:56:12: 3000000 INFO @ Sat, 03 Apr 2021 08:56:12: 8000000 INFO @ Sat, 03 Apr 2021 08:56:19: 12000000 INFO @ Sat, 03 Apr 2021 08:56:19: 9000000 INFO @ Sat, 03 Apr 2021 08:56:19: 4000000 INFO @ Sat, 03 Apr 2021 08:56:26: 10000000 INFO @ Sat, 03 Apr 2021 08:56:26: 13000000 INFO @ Sat, 03 Apr 2021 08:56:26: 5000000 INFO @ Sat, 03 Apr 2021 08:56:32: 11000000 INFO @ Sat, 03 Apr 2021 08:56:34: 14000000 INFO @ Sat, 03 Apr 2021 08:56:34: 6000000 INFO @ Sat, 03 Apr 2021 08:56:39: 12000000 INFO @ Sat, 03 Apr 2021 08:56:41: 7000000 INFO @ Sat, 03 Apr 2021 08:56:41: 15000000 INFO @ Sat, 03 Apr 2021 08:56:46: 13000000 INFO @ Sat, 03 Apr 2021 08:56:49: 8000000 INFO @ Sat, 03 Apr 2021 08:56:49: 16000000 INFO @ Sat, 03 Apr 2021 08:56:52: 14000000 INFO @ Sat, 03 Apr 2021 08:56:56: 9000000 INFO @ Sat, 03 Apr 2021 08:56:57: 17000000 INFO @ Sat, 03 Apr 2021 08:56:59: 15000000 INFO @ Sat, 03 Apr 2021 08:57:03: 10000000 INFO @ Sat, 03 Apr 2021 08:57:04: 18000000 INFO @ Sat, 03 Apr 2021 08:57:06: 16000000 INFO @ Sat, 03 Apr 2021 08:57:11: 11000000 INFO @ Sat, 03 Apr 2021 08:57:12: 19000000 INFO @ Sat, 03 Apr 2021 08:57:12: 17000000 INFO @ Sat, 03 Apr 2021 08:57:18: 12000000 INFO @ Sat, 03 Apr 2021 08:57:19: 18000000 INFO @ Sat, 03 Apr 2021 08:57:19: 20000000 INFO @ Sat, 03 Apr 2021 08:57:25: 19000000 INFO @ Sat, 03 Apr 2021 08:57:26: 13000000 INFO @ Sat, 03 Apr 2021 08:57:27: 21000000 INFO @ Sat, 03 Apr 2021 08:57:32: 20000000 INFO @ Sat, 03 Apr 2021 08:57:33: 14000000 INFO @ Sat, 03 Apr 2021 08:57:34: 22000000 INFO @ Sat, 03 Apr 2021 08:57:38: 21000000 INFO @ Sat, 03 Apr 2021 08:57:40: 15000000 INFO @ Sat, 03 Apr 2021 08:57:41: 23000000 INFO @ Sat, 03 Apr 2021 08:57:45: 22000000 INFO @ Sat, 03 Apr 2021 08:57:48: 16000000 INFO @ Sat, 03 Apr 2021 08:57:49: 24000000 INFO @ Sat, 03 Apr 2021 08:57:52: 23000000 INFO @ Sat, 03 Apr 2021 08:57:55: 17000000 INFO @ Sat, 03 Apr 2021 08:57:56: 25000000 INFO @ Sat, 03 Apr 2021 08:57:58: 24000000 INFO @ Sat, 03 Apr 2021 08:58:02: 18000000 INFO @ Sat, 03 Apr 2021 08:58:03: 26000000 INFO @ Sat, 03 Apr 2021 08:58:05: 25000000 INFO @ Sat, 03 Apr 2021 08:58:10: 19000000 INFO @ Sat, 03 Apr 2021 08:58:11: 27000000 INFO @ Sat, 03 Apr 2021 08:58:11: 26000000 INFO @ Sat, 03 Apr 2021 08:58:17: 20000000 INFO @ Sat, 03 Apr 2021 08:58:18: 27000000 INFO @ Sat, 03 Apr 2021 08:58:18: 28000000 INFO @ Sat, 03 Apr 2021 08:58:24: 21000000 INFO @ Sat, 03 Apr 2021 08:58:24: 28000000 INFO @ Sat, 03 Apr 2021 08:58:25: 29000000 INFO @ Sat, 03 Apr 2021 08:58:30: 29000000 INFO @ Sat, 03 Apr 2021 08:58:31: 22000000 INFO @ Sat, 03 Apr 2021 08:58:32: 30000000 INFO @ Sat, 03 Apr 2021 08:58:37: 30000000 INFO @ Sat, 03 Apr 2021 08:58:38: 23000000 INFO @ Sat, 03 Apr 2021 08:58:39: 31000000 INFO @ Sat, 03 Apr 2021 08:58:43: 31000000 INFO @ Sat, 03 Apr 2021 08:58:46: 24000000 INFO @ Sat, 03 Apr 2021 08:58:47: 32000000 INFO @ Sat, 03 Apr 2021 08:58:50: 32000000 INFO @ Sat, 03 Apr 2021 08:58:53: 25000000 INFO @ Sat, 03 Apr 2021 08:58:54: 33000000 INFO @ Sat, 03 Apr 2021 08:58:56: 33000000 INFO @ Sat, 03 Apr 2021 08:59:00: 26000000 INFO @ Sat, 03 Apr 2021 08:59:01: 34000000 INFO @ Sat, 03 Apr 2021 08:59:03: 34000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 08:59:07: 27000000 INFO @ Sat, 03 Apr 2021 08:59:09: 35000000 INFO @ Sat, 03 Apr 2021 08:59:09: 35000000 INFO @ Sat, 03 Apr 2021 08:59:14: 28000000 INFO @ Sat, 03 Apr 2021 08:59:16: 36000000 INFO @ Sat, 03 Apr 2021 08:59:16: 36000000 INFO @ Sat, 03 Apr 2021 08:59:21: 29000000 INFO @ Sat, 03 Apr 2021 08:59:23: 37000000 INFO @ Sat, 03 Apr 2021 08:59:24: 37000000 INFO @ Sat, 03 Apr 2021 08:59:27: #1 tag size is determined as 75 bps INFO @ Sat, 03 Apr 2021 08:59:27: #1 tag size = 75 INFO @ Sat, 03 Apr 2021 08:59:27: #1 total tags in treatment: 14454942 INFO @ Sat, 03 Apr 2021 08:59:27: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:59:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:59:27: #1 tags after filtering in treatment: 11529023 INFO @ Sat, 03 Apr 2021 08:59:27: #1 Redundant rate of treatment: 0.20 INFO @ Sat, 03 Apr 2021 08:59:27: #1 finished! INFO @ Sat, 03 Apr 2021 08:59:27: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:59:27: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:59:27: #1 tag size is determined as 75 bps INFO @ Sat, 03 Apr 2021 08:59:27: #1 tag size = 75 INFO @ Sat, 03 Apr 2021 08:59:27: #1 total tags in treatment: 14454942 INFO @ Sat, 03 Apr 2021 08:59:27: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:59:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:59:28: #1 tags after filtering in treatment: 11529023 INFO @ Sat, 03 Apr 2021 08:59:28: #1 Redundant rate of treatment: 0.20 INFO @ Sat, 03 Apr 2021 08:59:28: #1 finished! INFO @ Sat, 03 Apr 2021 08:59:28: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:59:28: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:59:28: #2 number of paired peaks: 192 WARNING @ Sat, 03 Apr 2021 08:59:28: Fewer paired peaks (192) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 192 pairs to build model! INFO @ Sat, 03 Apr 2021 08:59:28: start model_add_line... INFO @ Sat, 03 Apr 2021 08:59:28: start X-correlation... INFO @ Sat, 03 Apr 2021 08:59:28: end of X-cor INFO @ Sat, 03 Apr 2021 08:59:28: #2 finished! INFO @ Sat, 03 Apr 2021 08:59:28: #2 predicted fragment length is 84 bps INFO @ Sat, 03 Apr 2021 08:59:28: #2 alternative fragment length(s) may be 4,14,84,119,587 bps INFO @ Sat, 03 Apr 2021 08:59:28: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX7277033/SRX7277033.05_model.r WARNING @ Sat, 03 Apr 2021 08:59:28: #2 Since the d (84) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 08:59:28: #2 You may need to consider one of the other alternative d(s): 4,14,84,119,587 WARNING @ Sat, 03 Apr 2021 08:59:28: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 08:59:28: #3 Call peaks... INFO @ Sat, 03 Apr 2021 08:59:28: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 08:59:28: 30000000 INFO @ Sat, 03 Apr 2021 08:59:29: #2 number of paired peaks: 192 WARNING @ Sat, 03 Apr 2021 08:59:29: Fewer paired peaks (192) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 192 pairs to build model! INFO @ Sat, 03 Apr 2021 08:59:29: start model_add_line... INFO @ Sat, 03 Apr 2021 08:59:29: start X-correlation... INFO @ Sat, 03 Apr 2021 08:59:29: end of X-cor INFO @ Sat, 03 Apr 2021 08:59:29: #2 finished! INFO @ Sat, 03 Apr 2021 08:59:29: #2 predicted fragment length is 84 bps INFO @ Sat, 03 Apr 2021 08:59:29: #2 alternative fragment length(s) may be 4,14,84,119,587 bps INFO @ Sat, 03 Apr 2021 08:59:29: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX7277033/SRX7277033.10_model.r WARNING @ Sat, 03 Apr 2021 08:59:29: #2 Since the d (84) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 08:59:29: #2 You may need to consider one of the other alternative d(s): 4,14,84,119,587 WARNING @ Sat, 03 Apr 2021 08:59:29: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 08:59:29: #3 Call peaks... INFO @ Sat, 03 Apr 2021 08:59:29: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 08:59:35: 31000000 INFO @ Sat, 03 Apr 2021 08:59:42: 32000000 INFO @ Sat, 03 Apr 2021 08:59:49: 33000000 INFO @ Sat, 03 Apr 2021 08:59:56: 34000000 INFO @ Sat, 03 Apr 2021 08:59:56: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 08:59:56: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 09:00:02: 35000000 BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 09:00:09: 36000000 INFO @ Sat, 03 Apr 2021 09:00:10: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX7277033/SRX7277033.10_peaks.xls INFO @ Sat, 03 Apr 2021 09:00:10: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX7277033/SRX7277033.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 09:00:10: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX7277033/SRX7277033.10_summits.bed INFO @ Sat, 03 Apr 2021 09:00:10: Done! pass1 - making usageList (13 chroms): 1 millis pass2 - checking and writing primary data (1477 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 09:00:10: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX7277033/SRX7277033.05_peaks.xls INFO @ Sat, 03 Apr 2021 09:00:10: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX7277033/SRX7277033.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 09:00:10: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX7277033/SRX7277033.05_summits.bed INFO @ Sat, 03 Apr 2021 09:00:10: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (6773 records, 4 fields): 8 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 09:00:16: 37000000 INFO @ Sat, 03 Apr 2021 09:00:19: #1 tag size is determined as 75 bps INFO @ Sat, 03 Apr 2021 09:00:19: #1 tag size = 75 INFO @ Sat, 03 Apr 2021 09:00:19: #1 total tags in treatment: 14454942 INFO @ Sat, 03 Apr 2021 09:00:19: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 09:00:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 09:00:20: #1 tags after filtering in treatment: 11529023 INFO @ Sat, 03 Apr 2021 09:00:20: #1 Redundant rate of treatment: 0.20 INFO @ Sat, 03 Apr 2021 09:00:20: #1 finished! INFO @ Sat, 03 Apr 2021 09:00:20: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 09:00:20: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 09:00:21: #2 number of paired peaks: 192 WARNING @ Sat, 03 Apr 2021 09:00:21: Fewer paired peaks (192) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 192 pairs to build model! INFO @ Sat, 03 Apr 2021 09:00:21: start model_add_line... INFO @ Sat, 03 Apr 2021 09:00:21: start X-correlation... INFO @ Sat, 03 Apr 2021 09:00:21: end of X-cor INFO @ Sat, 03 Apr 2021 09:00:21: #2 finished! INFO @ Sat, 03 Apr 2021 09:00:21: #2 predicted fragment length is 84 bps INFO @ Sat, 03 Apr 2021 09:00:21: #2 alternative fragment length(s) may be 4,14,84,119,587 bps INFO @ Sat, 03 Apr 2021 09:00:21: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX7277033/SRX7277033.20_model.r WARNING @ Sat, 03 Apr 2021 09:00:21: #2 Since the d (84) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 09:00:21: #2 You may need to consider one of the other alternative d(s): 4,14,84,119,587 WARNING @ Sat, 03 Apr 2021 09:00:21: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 09:00:21: #3 Call peaks... INFO @ Sat, 03 Apr 2021 09:00:21: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 09:00:48: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 09:01:02: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX7277033/SRX7277033.20_peaks.xls INFO @ Sat, 03 Apr 2021 09:01:02: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX7277033/SRX7277033.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 09:01:02: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX7277033/SRX7277033.20_summits.bed INFO @ Sat, 03 Apr 2021 09:01:02: Done! pass1 - making usageList (8 chroms): 0 millis pass2 - checking and writing primary data (224 records, 4 fields): 3 millis CompletedMACS2peakCalling