Job ID = 6626563 SRX = SRX7224532 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 25635585 spots for SRR10541172/SRR10541172.sra Written 25635585 spots for SRR10541172/SRR10541172.sra fastq に変換しました。 bowtie でマッピング中... Your job 6626852 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:45:52 25635585 reads; of these: 25635585 (100.00%) were paired; of these: 1698730 (6.63%) aligned concordantly 0 times 20911344 (81.57%) aligned concordantly exactly 1 time 3025511 (11.80%) aligned concordantly >1 times ---- 1698730 pairs aligned concordantly 0 times; of these: 95599 (5.63%) aligned discordantly 1 time ---- 1603131 pairs aligned 0 times concordantly or discordantly; of these: 3206262 mates make up the pairs; of these: 2026814 (63.21%) aligned 0 times 754681 (23.54%) aligned exactly 1 time 424767 (13.25%) aligned >1 times 96.05% overall alignment rate Time searching: 00:45:52 Overall time: 00:45:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 548093 / 23954509 = 0.0229 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:35:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7224532/SRX7224532.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7224532/SRX7224532.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7224532/SRX7224532.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7224532/SRX7224532.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:35:26: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:35:26: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:35:33: 1000000 INFO @ Tue, 14 Jul 2020 08:35:39: 2000000 INFO @ Tue, 14 Jul 2020 08:35:46: 3000000 INFO @ Tue, 14 Jul 2020 08:35:52: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:35:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7224532/SRX7224532.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7224532/SRX7224532.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7224532/SRX7224532.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7224532/SRX7224532.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:35:56: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:35:56: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:35:59: 5000000 INFO @ Tue, 14 Jul 2020 08:36:04: 1000000 INFO @ Tue, 14 Jul 2020 08:36:07: 6000000 INFO @ Tue, 14 Jul 2020 08:36:13: 2000000 INFO @ Tue, 14 Jul 2020 08:36:15: 7000000 INFO @ Tue, 14 Jul 2020 08:36:21: 3000000 INFO @ Tue, 14 Jul 2020 08:36:24: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:36:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7224532/SRX7224532.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7224532/SRX7224532.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7224532/SRX7224532.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7224532/SRX7224532.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:36:26: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:36:26: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:36:29: 4000000 INFO @ Tue, 14 Jul 2020 08:36:32: 9000000 INFO @ Tue, 14 Jul 2020 08:36:35: 1000000 INFO @ Tue, 14 Jul 2020 08:36:38: 5000000 INFO @ Tue, 14 Jul 2020 08:36:40: 10000000 INFO @ Tue, 14 Jul 2020 08:36:44: 2000000 INFO @ Tue, 14 Jul 2020 08:36:47: 6000000 INFO @ Tue, 14 Jul 2020 08:36:49: 11000000 INFO @ Tue, 14 Jul 2020 08:36:53: 3000000 INFO @ Tue, 14 Jul 2020 08:36:56: 7000000 INFO @ Tue, 14 Jul 2020 08:36:57: 12000000 INFO @ Tue, 14 Jul 2020 08:37:02: 4000000 INFO @ Tue, 14 Jul 2020 08:37:05: 8000000 INFO @ Tue, 14 Jul 2020 08:37:06: 13000000 INFO @ Tue, 14 Jul 2020 08:37:11: 5000000 INFO @ Tue, 14 Jul 2020 08:37:14: 9000000 INFO @ Tue, 14 Jul 2020 08:37:14: 14000000 INFO @ Tue, 14 Jul 2020 08:37:19: 6000000 INFO @ Tue, 14 Jul 2020 08:37:22: 10000000 INFO @ Tue, 14 Jul 2020 08:37:23: 15000000 INFO @ Tue, 14 Jul 2020 08:37:28: 7000000 INFO @ Tue, 14 Jul 2020 08:37:31: 11000000 INFO @ Tue, 14 Jul 2020 08:37:32: 16000000 INFO @ Tue, 14 Jul 2020 08:37:37: 8000000 INFO @ Tue, 14 Jul 2020 08:37:40: 12000000 INFO @ Tue, 14 Jul 2020 08:37:40: 17000000 INFO @ Tue, 14 Jul 2020 08:37:45: 9000000 INFO @ Tue, 14 Jul 2020 08:37:48: 13000000 INFO @ Tue, 14 Jul 2020 08:37:49: 18000000 INFO @ Tue, 14 Jul 2020 08:37:54: 10000000 INFO @ Tue, 14 Jul 2020 08:37:57: 14000000 INFO @ Tue, 14 Jul 2020 08:37:58: 19000000 INFO @ Tue, 14 Jul 2020 08:38:02: 11000000 INFO @ Tue, 14 Jul 2020 08:38:06: 15000000 INFO @ Tue, 14 Jul 2020 08:38:06: 20000000 INFO @ Tue, 14 Jul 2020 08:38:11: 12000000 INFO @ Tue, 14 Jul 2020 08:38:15: 16000000 INFO @ Tue, 14 Jul 2020 08:38:15: 21000000 INFO @ Tue, 14 Jul 2020 08:38:19: 13000000 INFO @ Tue, 14 Jul 2020 08:38:24: 17000000 INFO @ Tue, 14 Jul 2020 08:38:24: 22000000 INFO @ Tue, 14 Jul 2020 08:38:28: 14000000 INFO @ Tue, 14 Jul 2020 08:38:32: 18000000 INFO @ Tue, 14 Jul 2020 08:38:33: 23000000 INFO @ Tue, 14 Jul 2020 08:38:36: 15000000 INFO @ Tue, 14 Jul 2020 08:38:41: 19000000 INFO @ Tue, 14 Jul 2020 08:38:41: 24000000 INFO @ Tue, 14 Jul 2020 08:38:45: 16000000 INFO @ Tue, 14 Jul 2020 08:38:50: 20000000 INFO @ Tue, 14 Jul 2020 08:38:50: 25000000 INFO @ Tue, 14 Jul 2020 08:38:53: 17000000 INFO @ Tue, 14 Jul 2020 08:38:58: 21000000 INFO @ Tue, 14 Jul 2020 08:38:59: 26000000 INFO @ Tue, 14 Jul 2020 08:39:01: 18000000 INFO @ Tue, 14 Jul 2020 08:39:07: 22000000 INFO @ Tue, 14 Jul 2020 08:39:08: 27000000 INFO @ Tue, 14 Jul 2020 08:39:10: 19000000 INFO @ Tue, 14 Jul 2020 08:39:16: 23000000 INFO @ Tue, 14 Jul 2020 08:39:16: 28000000 INFO @ Tue, 14 Jul 2020 08:39:18: 20000000 INFO @ Tue, 14 Jul 2020 08:39:25: 24000000 INFO @ Tue, 14 Jul 2020 08:39:25: 29000000 INFO @ Tue, 14 Jul 2020 08:39:26: 21000000 INFO @ Tue, 14 Jul 2020 08:39:33: 25000000 INFO @ Tue, 14 Jul 2020 08:39:34: 30000000 INFO @ Tue, 14 Jul 2020 08:39:35: 22000000 INFO @ Tue, 14 Jul 2020 08:39:42: 26000000 INFO @ Tue, 14 Jul 2020 08:39:42: 31000000 INFO @ Tue, 14 Jul 2020 08:39:43: 23000000 INFO @ Tue, 14 Jul 2020 08:39:51: 27000000 INFO @ Tue, 14 Jul 2020 08:39:51: 32000000 INFO @ Tue, 14 Jul 2020 08:39:51: 24000000 INFO @ Tue, 14 Jul 2020 08:39:59: 28000000 INFO @ Tue, 14 Jul 2020 08:40:00: 25000000 INFO @ Tue, 14 Jul 2020 08:40:00: 33000000 INFO @ Tue, 14 Jul 2020 08:40:08: 29000000 INFO @ Tue, 14 Jul 2020 08:40:08: 26000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 08:40:09: 34000000 INFO @ Tue, 14 Jul 2020 08:40:17: 27000000 INFO @ Tue, 14 Jul 2020 08:40:17: 30000000 INFO @ Tue, 14 Jul 2020 08:40:17: 35000000 INFO @ Tue, 14 Jul 2020 08:40:25: 28000000 INFO @ Tue, 14 Jul 2020 08:40:25: 31000000 INFO @ Tue, 14 Jul 2020 08:40:26: 36000000 INFO @ Tue, 14 Jul 2020 08:40:34: 29000000 INFO @ Tue, 14 Jul 2020 08:40:34: 32000000 INFO @ Tue, 14 Jul 2020 08:40:34: 37000000 INFO @ Tue, 14 Jul 2020 08:40:42: 33000000 INFO @ Tue, 14 Jul 2020 08:40:42: 30000000 INFO @ Tue, 14 Jul 2020 08:40:43: 38000000 INFO @ Tue, 14 Jul 2020 08:40:51: 34000000 INFO @ Tue, 14 Jul 2020 08:40:51: 31000000 INFO @ Tue, 14 Jul 2020 08:40:52: 39000000 INFO @ Tue, 14 Jul 2020 08:40:59: 35000000 INFO @ Tue, 14 Jul 2020 08:40:59: 32000000 INFO @ Tue, 14 Jul 2020 08:41:00: 40000000 INFO @ Tue, 14 Jul 2020 08:41:08: 36000000 INFO @ Tue, 14 Jul 2020 08:41:08: 33000000 INFO @ Tue, 14 Jul 2020 08:41:09: 41000000 INFO @ Tue, 14 Jul 2020 08:41:16: 37000000 INFO @ Tue, 14 Jul 2020 08:41:17: 34000000 INFO @ Tue, 14 Jul 2020 08:41:18: 42000000 INFO @ Tue, 14 Jul 2020 08:41:25: 35000000 INFO @ Tue, 14 Jul 2020 08:41:25: 38000000 INFO @ Tue, 14 Jul 2020 08:41:27: 43000000 BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 08:41:33: 36000000 INFO @ Tue, 14 Jul 2020 08:41:34: 39000000 INFO @ Tue, 14 Jul 2020 08:41:35: 44000000 INFO @ Tue, 14 Jul 2020 08:41:42: 37000000 INFO @ Tue, 14 Jul 2020 08:41:43: 40000000 INFO @ Tue, 14 Jul 2020 08:41:44: 45000000 INFO @ Tue, 14 Jul 2020 08:41:50: 38000000 INFO @ Tue, 14 Jul 2020 08:41:52: 41000000 INFO @ Tue, 14 Jul 2020 08:41:53: 46000000 INFO @ Tue, 14 Jul 2020 08:41:59: 39000000 INFO @ Tue, 14 Jul 2020 08:42:00: 42000000 INFO @ Tue, 14 Jul 2020 08:42:02: 47000000 INFO @ Tue, 14 Jul 2020 08:42:08: 40000000 INFO @ Tue, 14 Jul 2020 08:42:09: 43000000 INFO @ Tue, 14 Jul 2020 08:42:10: 48000000 INFO @ Tue, 14 Jul 2020 08:42:12: #1 tag size is determined as 78 bps INFO @ Tue, 14 Jul 2020 08:42:12: #1 tag size = 78 INFO @ Tue, 14 Jul 2020 08:42:12: #1 total tags in treatment: 23389350 INFO @ Tue, 14 Jul 2020 08:42:12: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:42:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:42:12: #1 tags after filtering in treatment: 21958267 INFO @ Tue, 14 Jul 2020 08:42:12: #1 Redundant rate of treatment: 0.06 INFO @ Tue, 14 Jul 2020 08:42:12: #1 finished! INFO @ Tue, 14 Jul 2020 08:42:12: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:42:12: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:42:13: #2 number of paired peaks: 45 WARNING @ Tue, 14 Jul 2020 08:42:13: Too few paired peaks (45) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:42:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7224532/SRX7224532.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7224532/SRX7224532.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7224532/SRX7224532.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7224532/SRX7224532.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:42:16: 41000000 INFO @ Tue, 14 Jul 2020 08:42:18: 44000000 INFO @ Tue, 14 Jul 2020 08:42:25: 42000000 INFO @ Tue, 14 Jul 2020 08:42:26: 45000000 INFO @ Tue, 14 Jul 2020 08:42:33: 43000000 INFO @ Tue, 14 Jul 2020 08:42:35: 46000000 INFO @ Tue, 14 Jul 2020 08:42:42: 44000000 INFO @ Tue, 14 Jul 2020 08:42:43: 47000000 INFO @ Tue, 14 Jul 2020 08:42:50: 45000000 INFO @ Tue, 14 Jul 2020 08:42:51: 48000000 INFO @ Tue, 14 Jul 2020 08:42:52: #1 tag size is determined as 78 bps INFO @ Tue, 14 Jul 2020 08:42:52: #1 tag size = 78 INFO @ Tue, 14 Jul 2020 08:42:52: #1 total tags in treatment: 23389350 INFO @ Tue, 14 Jul 2020 08:42:52: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:42:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:42:53: #1 tags after filtering in treatment: 21958267 INFO @ Tue, 14 Jul 2020 08:42:53: #1 Redundant rate of treatment: 0.06 INFO @ Tue, 14 Jul 2020 08:42:53: #1 finished! INFO @ Tue, 14 Jul 2020 08:42:53: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:42:53: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:42:54: #2 number of paired peaks: 45 WARNING @ Tue, 14 Jul 2020 08:42:54: Too few paired peaks (45) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:42:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7224532/SRX7224532.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7224532/SRX7224532.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7224532/SRX7224532.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7224532/SRX7224532.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:42:58: 46000000 INFO @ Tue, 14 Jul 2020 08:43:06: 47000000 INFO @ Tue, 14 Jul 2020 08:43:13: 48000000 INFO @ Tue, 14 Jul 2020 08:43:15: #1 tag size is determined as 78 bps INFO @ Tue, 14 Jul 2020 08:43:15: #1 tag size = 78 INFO @ Tue, 14 Jul 2020 08:43:15: #1 total tags in treatment: 23389350 INFO @ Tue, 14 Jul 2020 08:43:15: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:43:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:43:15: #1 tags after filtering in treatment: 21958267 INFO @ Tue, 14 Jul 2020 08:43:15: #1 Redundant rate of treatment: 0.06 INFO @ Tue, 14 Jul 2020 08:43:15: #1 finished! INFO @ Tue, 14 Jul 2020 08:43:15: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:43:15: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:43:16: #2 number of paired peaks: 45 WARNING @ Tue, 14 Jul 2020 08:43:16: Too few paired peaks (45) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:43:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7224532/SRX7224532.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7224532/SRX7224532.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7224532/SRX7224532.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7224532/SRX7224532.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling