Job ID = 8069494 SRX = SRX7191477 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-08-08T03:33:44 prefetch.2.10.7: 1) Downloading 'SRR10502461'... 2020-08-08T03:33:44 prefetch.2.10.7: Downloading via HTTPS... 2020-08-08T03:34:51 prefetch.2.10.7: HTTPS download succeed 2020-08-08T03:34:51 prefetch.2.10.7: 'SRR10502461' is valid 2020-08-08T03:34:51 prefetch.2.10.7: 1) 'SRR10502461' was downloaded successfully Read 17631331 spots for SRR10502461/SRR10502461.sra Written 17631331 spots for SRR10502461/SRR10502461.sra fastq に変換しました。 bowtie でマッピング中... Your job 8069966 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:18 17631331 reads; of these: 17631331 (100.00%) were unpaired; of these: 2797242 (15.87%) aligned 0 times 10976911 (62.26%) aligned exactly 1 time 3857178 (21.88%) aligned >1 times 84.13% overall alignment rate Time searching: 00:05:18 Overall time: 00:05:18 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1782245 / 14834089 = 0.1201 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 08 Aug 2020 12:44:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7191477/SRX7191477.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7191477/SRX7191477.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7191477/SRX7191477.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7191477/SRX7191477.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Aug 2020 12:44:24: #1 read tag files... INFO @ Sat, 08 Aug 2020 12:44:24: #1 read treatment tags... INFO @ Sat, 08 Aug 2020 12:44:30: 1000000 INFO @ Sat, 08 Aug 2020 12:44:36: 2000000 INFO @ Sat, 08 Aug 2020 12:44:42: 3000000 INFO @ Sat, 08 Aug 2020 12:44:49: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 08 Aug 2020 12:44:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7191477/SRX7191477.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7191477/SRX7191477.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7191477/SRX7191477.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7191477/SRX7191477.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Aug 2020 12:44:54: #1 read tag files... INFO @ Sat, 08 Aug 2020 12:44:54: #1 read treatment tags... INFO @ Sat, 08 Aug 2020 12:44:55: 5000000 INFO @ Sat, 08 Aug 2020 12:45:01: 1000000 INFO @ Sat, 08 Aug 2020 12:45:02: 6000000 INFO @ Sat, 08 Aug 2020 12:45:08: 2000000 INFO @ Sat, 08 Aug 2020 12:45:08: 7000000 INFO @ Sat, 08 Aug 2020 12:45:14: 3000000 INFO @ Sat, 08 Aug 2020 12:45:15: 8000000 INFO @ Sat, 08 Aug 2020 12:45:21: 4000000 INFO @ Sat, 08 Aug 2020 12:45:21: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 08 Aug 2020 12:45:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7191477/SRX7191477.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7191477/SRX7191477.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7191477/SRX7191477.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7191477/SRX7191477.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Aug 2020 12:45:24: #1 read tag files... INFO @ Sat, 08 Aug 2020 12:45:24: #1 read treatment tags... INFO @ Sat, 08 Aug 2020 12:45:28: 5000000 INFO @ Sat, 08 Aug 2020 12:45:28: 10000000 INFO @ Sat, 08 Aug 2020 12:45:30: 1000000 INFO @ Sat, 08 Aug 2020 12:45:34: 6000000 INFO @ Sat, 08 Aug 2020 12:45:35: 11000000 INFO @ Sat, 08 Aug 2020 12:45:36: 2000000 INFO @ Sat, 08 Aug 2020 12:45:41: 12000000 INFO @ Sat, 08 Aug 2020 12:45:41: 7000000 INFO @ Sat, 08 Aug 2020 12:45:42: 3000000 INFO @ Sat, 08 Aug 2020 12:45:48: 4000000 INFO @ Sat, 08 Aug 2020 12:45:48: 8000000 INFO @ Sat, 08 Aug 2020 12:45:48: 13000000 INFO @ Sat, 08 Aug 2020 12:45:48: #1 tag size is determined as 50 bps INFO @ Sat, 08 Aug 2020 12:45:48: #1 tag size = 50 INFO @ Sat, 08 Aug 2020 12:45:48: #1 total tags in treatment: 13051844 INFO @ Sat, 08 Aug 2020 12:45:48: #1 user defined the maximum tags... INFO @ Sat, 08 Aug 2020 12:45:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Aug 2020 12:45:48: #1 tags after filtering in treatment: 13051844 INFO @ Sat, 08 Aug 2020 12:45:48: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Aug 2020 12:45:48: #1 finished! INFO @ Sat, 08 Aug 2020 12:45:48: #2 Build Peak Model... INFO @ Sat, 08 Aug 2020 12:45:48: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Aug 2020 12:45:49: #2 number of paired peaks: 69 WARNING @ Sat, 08 Aug 2020 12:45:49: Too few paired peaks (69) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Aug 2020 12:45:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7191477/SRX7191477.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7191477/SRX7191477.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7191477/SRX7191477.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7191477/SRX7191477.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 08 Aug 2020 12:45:54: 5000000 INFO @ Sat, 08 Aug 2020 12:45:54: 9000000 INFO @ Sat, 08 Aug 2020 12:45:59: 6000000 INFO @ Sat, 08 Aug 2020 12:46:00: 10000000 INFO @ Sat, 08 Aug 2020 12:46:05: 7000000 INFO @ Sat, 08 Aug 2020 12:46:07: 11000000 INFO @ Sat, 08 Aug 2020 12:46:11: 8000000 INFO @ Sat, 08 Aug 2020 12:46:13: 12000000 INFO @ Sat, 08 Aug 2020 12:46:17: 9000000 INFO @ Sat, 08 Aug 2020 12:46:20: 13000000 INFO @ Sat, 08 Aug 2020 12:46:20: #1 tag size is determined as 50 bps INFO @ Sat, 08 Aug 2020 12:46:20: #1 tag size = 50 INFO @ Sat, 08 Aug 2020 12:46:20: #1 total tags in treatment: 13051844 INFO @ Sat, 08 Aug 2020 12:46:20: #1 user defined the maximum tags... INFO @ Sat, 08 Aug 2020 12:46:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Aug 2020 12:46:20: #1 tags after filtering in treatment: 13051844 INFO @ Sat, 08 Aug 2020 12:46:20: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Aug 2020 12:46:20: #1 finished! INFO @ Sat, 08 Aug 2020 12:46:20: #2 Build Peak Model... INFO @ Sat, 08 Aug 2020 12:46:20: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Aug 2020 12:46:21: #2 number of paired peaks: 69 WARNING @ Sat, 08 Aug 2020 12:46:21: Too few paired peaks (69) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Aug 2020 12:46:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7191477/SRX7191477.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7191477/SRX7191477.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7191477/SRX7191477.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7191477/SRX7191477.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 08 Aug 2020 12:46:23: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 08 Aug 2020 12:46:28: 11000000 INFO @ Sat, 08 Aug 2020 12:46:34: 12000000 INFO @ Sat, 08 Aug 2020 12:46:39: 13000000 INFO @ Sat, 08 Aug 2020 12:46:40: #1 tag size is determined as 50 bps INFO @ Sat, 08 Aug 2020 12:46:40: #1 tag size = 50 INFO @ Sat, 08 Aug 2020 12:46:40: #1 total tags in treatment: 13051844 INFO @ Sat, 08 Aug 2020 12:46:40: #1 user defined the maximum tags... INFO @ Sat, 08 Aug 2020 12:46:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Aug 2020 12:46:40: #1 tags after filtering in treatment: 13051844 INFO @ Sat, 08 Aug 2020 12:46:40: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Aug 2020 12:46:40: #1 finished! INFO @ Sat, 08 Aug 2020 12:46:40: #2 Build Peak Model... INFO @ Sat, 08 Aug 2020 12:46:40: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Aug 2020 12:46:41: #2 number of paired peaks: 69 WARNING @ Sat, 08 Aug 2020 12:46:41: Too few paired peaks (69) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Aug 2020 12:46:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7191477/SRX7191477.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7191477/SRX7191477.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7191477/SRX7191477.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7191477/SRX7191477.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。