Job ID = 8069479 SRX = SRX7191472 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-08-08T03:33:29 prefetch.2.10.7: 1) Downloading 'SRR10502456'... 2020-08-08T03:33:29 prefetch.2.10.7: Downloading via HTTPS... 2020-08-08T03:34:55 prefetch.2.10.7: HTTPS download succeed 2020-08-08T03:34:55 prefetch.2.10.7: 1) 'SRR10502456' was downloaded successfully Read 23148804 spots for SRR10502456/SRR10502456.sra Written 23148804 spots for SRR10502456/SRR10502456.sra fastq に変換しました。 bowtie でマッピング中... Your job 8070095 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:49 23148804 reads; of these: 23148804 (100.00%) were unpaired; of these: 725913 (3.14%) aligned 0 times 16301985 (70.42%) aligned exactly 1 time 6120906 (26.44%) aligned >1 times 96.86% overall alignment rate Time searching: 00:07:49 Overall time: 00:07:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 1627751 / 22422891 = 0.0726 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 08 Aug 2020 12:48:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7191472/SRX7191472.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7191472/SRX7191472.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7191472/SRX7191472.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7191472/SRX7191472.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Aug 2020 12:48:37: #1 read tag files... INFO @ Sat, 08 Aug 2020 12:48:37: #1 read treatment tags... INFO @ Sat, 08 Aug 2020 12:48:43: 1000000 INFO @ Sat, 08 Aug 2020 12:48:48: 2000000 INFO @ Sat, 08 Aug 2020 12:48:53: 3000000 INFO @ Sat, 08 Aug 2020 12:48:58: 4000000 INFO @ Sat, 08 Aug 2020 12:49:03: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 08 Aug 2020 12:49:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7191472/SRX7191472.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7191472/SRX7191472.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7191472/SRX7191472.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7191472/SRX7191472.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Aug 2020 12:49:07: #1 read tag files... INFO @ Sat, 08 Aug 2020 12:49:07: #1 read treatment tags... INFO @ Sat, 08 Aug 2020 12:49:09: 6000000 INFO @ Sat, 08 Aug 2020 12:49:13: 1000000 INFO @ Sat, 08 Aug 2020 12:49:15: 7000000 INFO @ Sat, 08 Aug 2020 12:49:18: 2000000 INFO @ Sat, 08 Aug 2020 12:49:21: 8000000 INFO @ Sat, 08 Aug 2020 12:49:24: 3000000 INFO @ Sat, 08 Aug 2020 12:49:26: 9000000 INFO @ Sat, 08 Aug 2020 12:49:30: 4000000 INFO @ Sat, 08 Aug 2020 12:49:33: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 08 Aug 2020 12:49:36: 5000000 INFO @ Sat, 08 Aug 2020 12:49:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7191472/SRX7191472.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7191472/SRX7191472.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7191472/SRX7191472.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7191472/SRX7191472.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Aug 2020 12:49:37: #1 read tag files... INFO @ Sat, 08 Aug 2020 12:49:37: #1 read treatment tags... INFO @ Sat, 08 Aug 2020 12:49:39: 11000000 INFO @ Sat, 08 Aug 2020 12:49:43: 6000000 INFO @ Sat, 08 Aug 2020 12:49:44: 1000000 INFO @ Sat, 08 Aug 2020 12:49:45: 12000000 INFO @ Sat, 08 Aug 2020 12:49:49: 7000000 INFO @ Sat, 08 Aug 2020 12:49:51: 2000000 INFO @ Sat, 08 Aug 2020 12:49:52: 13000000 INFO @ Sat, 08 Aug 2020 12:49:55: 8000000 INFO @ Sat, 08 Aug 2020 12:49:58: 3000000 INFO @ Sat, 08 Aug 2020 12:49:58: 14000000 INFO @ Sat, 08 Aug 2020 12:50:02: 9000000 INFO @ Sat, 08 Aug 2020 12:50:04: 15000000 INFO @ Sat, 08 Aug 2020 12:50:05: 4000000 INFO @ Sat, 08 Aug 2020 12:50:08: 10000000 INFO @ Sat, 08 Aug 2020 12:50:11: 16000000 INFO @ Sat, 08 Aug 2020 12:50:12: 5000000 INFO @ Sat, 08 Aug 2020 12:50:14: 11000000 INFO @ Sat, 08 Aug 2020 12:50:17: 17000000 INFO @ Sat, 08 Aug 2020 12:50:19: 6000000 INFO @ Sat, 08 Aug 2020 12:50:20: 12000000 INFO @ Sat, 08 Aug 2020 12:50:23: 18000000 INFO @ Sat, 08 Aug 2020 12:50:26: 7000000 INFO @ Sat, 08 Aug 2020 12:50:27: 13000000 INFO @ Sat, 08 Aug 2020 12:50:30: 19000000 INFO @ Sat, 08 Aug 2020 12:50:33: 8000000 INFO @ Sat, 08 Aug 2020 12:50:33: 14000000 INFO @ Sat, 08 Aug 2020 12:50:36: 20000000 INFO @ Sat, 08 Aug 2020 12:50:39: 9000000 INFO @ Sat, 08 Aug 2020 12:50:40: 15000000 INFO @ Sat, 08 Aug 2020 12:50:41: #1 tag size is determined as 50 bps INFO @ Sat, 08 Aug 2020 12:50:41: #1 tag size = 50 INFO @ Sat, 08 Aug 2020 12:50:41: #1 total tags in treatment: 20795140 INFO @ Sat, 08 Aug 2020 12:50:41: #1 user defined the maximum tags... INFO @ Sat, 08 Aug 2020 12:50:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Aug 2020 12:50:42: #1 tags after filtering in treatment: 20795140 INFO @ Sat, 08 Aug 2020 12:50:42: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Aug 2020 12:50:42: #1 finished! INFO @ Sat, 08 Aug 2020 12:50:42: #2 Build Peak Model... INFO @ Sat, 08 Aug 2020 12:50:42: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Aug 2020 12:50:43: #2 number of paired peaks: 65 WARNING @ Sat, 08 Aug 2020 12:50:43: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Aug 2020 12:50:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7191472/SRX7191472.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7191472/SRX7191472.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7191472/SRX7191472.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7191472/SRX7191472.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 08 Aug 2020 12:50:46: 16000000 INFO @ Sat, 08 Aug 2020 12:50:47: 10000000 INFO @ Sat, 08 Aug 2020 12:50:52: 17000000 INFO @ Sat, 08 Aug 2020 12:50:54: 11000000 INFO @ Sat, 08 Aug 2020 12:50:59: 18000000 INFO @ Sat, 08 Aug 2020 12:51:01: 12000000 INFO @ Sat, 08 Aug 2020 12:51:05: 19000000 INFO @ Sat, 08 Aug 2020 12:51:08: 13000000 INFO @ Sat, 08 Aug 2020 12:51:11: 20000000 INFO @ Sat, 08 Aug 2020 12:51:15: 14000000 INFO @ Sat, 08 Aug 2020 12:51:16: #1 tag size is determined as 50 bps INFO @ Sat, 08 Aug 2020 12:51:16: #1 tag size = 50 INFO @ Sat, 08 Aug 2020 12:51:16: #1 total tags in treatment: 20795140 INFO @ Sat, 08 Aug 2020 12:51:16: #1 user defined the maximum tags... INFO @ Sat, 08 Aug 2020 12:51:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Aug 2020 12:51:17: #1 tags after filtering in treatment: 20795140 INFO @ Sat, 08 Aug 2020 12:51:17: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Aug 2020 12:51:17: #1 finished! INFO @ Sat, 08 Aug 2020 12:51:17: #2 Build Peak Model... INFO @ Sat, 08 Aug 2020 12:51:17: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Aug 2020 12:51:18: #2 number of paired peaks: 65 WARNING @ Sat, 08 Aug 2020 12:51:18: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Aug 2020 12:51:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7191472/SRX7191472.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7191472/SRX7191472.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7191472/SRX7191472.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7191472/SRX7191472.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 08 Aug 2020 12:51:22: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 08 Aug 2020 12:51:29: 16000000 INFO @ Sat, 08 Aug 2020 12:51:36: 17000000 INFO @ Sat, 08 Aug 2020 12:51:42: 18000000 INFO @ Sat, 08 Aug 2020 12:51:49: 19000000 INFO @ Sat, 08 Aug 2020 12:51:55: 20000000 INFO @ Sat, 08 Aug 2020 12:52:01: #1 tag size is determined as 50 bps INFO @ Sat, 08 Aug 2020 12:52:01: #1 tag size = 50 INFO @ Sat, 08 Aug 2020 12:52:01: #1 total tags in treatment: 20795140 INFO @ Sat, 08 Aug 2020 12:52:01: #1 user defined the maximum tags... INFO @ Sat, 08 Aug 2020 12:52:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Aug 2020 12:52:01: #1 tags after filtering in treatment: 20795140 INFO @ Sat, 08 Aug 2020 12:52:01: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Aug 2020 12:52:01: #1 finished! INFO @ Sat, 08 Aug 2020 12:52:01: #2 Build Peak Model... INFO @ Sat, 08 Aug 2020 12:52:01: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Aug 2020 12:52:02: #2 number of paired peaks: 65 WARNING @ Sat, 08 Aug 2020 12:52:02: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Aug 2020 12:52:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7191472/SRX7191472.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7191472/SRX7191472.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7191472/SRX7191472.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7191472/SRX7191472.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。