Job ID = 14168222 SRX = SRX7175252 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 27027725 spots for SRR10485692/SRR10485692.sra Written 27027725 spots for SRR10485692/SRR10485692.sra fastq に変換しました。 bowtie でマッピング中... Your job 14169445 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:25:05 27027725 reads; of these: 27027725 (100.00%) were paired; of these: 1640626 (6.07%) aligned concordantly 0 times 18142830 (67.13%) aligned concordantly exactly 1 time 7244269 (26.80%) aligned concordantly >1 times ---- 1640626 pairs aligned concordantly 0 times; of these: 373388 (22.76%) aligned discordantly 1 time ---- 1267238 pairs aligned 0 times concordantly or discordantly; of these: 2534476 mates make up the pairs; of these: 1240223 (48.93%) aligned 0 times 738253 (29.13%) aligned exactly 1 time 556000 (21.94%) aligned >1 times 97.71% overall alignment rate Time searching: 01:25:05 Overall time: 01:25:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 1325436 / 25704427 = 0.0516 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 18:00:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7175252/SRX7175252.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7175252/SRX7175252.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7175252/SRX7175252.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7175252/SRX7175252.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 18:00:57: #1 read tag files... INFO @ Fri, 10 Dec 2021 18:00:57: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 18:01:08: 1000000 INFO @ Fri, 10 Dec 2021 18:01:19: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 18:01:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7175252/SRX7175252.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7175252/SRX7175252.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7175252/SRX7175252.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7175252/SRX7175252.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 18:01:25: #1 read tag files... INFO @ Fri, 10 Dec 2021 18:01:25: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 18:01:30: 3000000 INFO @ Fri, 10 Dec 2021 18:01:35: 1000000 INFO @ Fri, 10 Dec 2021 18:01:42: 4000000 INFO @ Fri, 10 Dec 2021 18:01:45: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 18:01:54: 5000000 INFO @ Fri, 10 Dec 2021 18:01:55: 3000000 INFO @ Fri, 10 Dec 2021 18:01:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7175252/SRX7175252.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7175252/SRX7175252.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7175252/SRX7175252.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7175252/SRX7175252.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 18:01:55: #1 read tag files... INFO @ Fri, 10 Dec 2021 18:01:55: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 18:02:05: 6000000 INFO @ Fri, 10 Dec 2021 18:02:06: 4000000 INFO @ Fri, 10 Dec 2021 18:02:08: 1000000 INFO @ Fri, 10 Dec 2021 18:02:16: 7000000 INFO @ Fri, 10 Dec 2021 18:02:16: 5000000 INFO @ Fri, 10 Dec 2021 18:02:20: 2000000 INFO @ Fri, 10 Dec 2021 18:02:27: 8000000 INFO @ Fri, 10 Dec 2021 18:02:27: 6000000 INFO @ Fri, 10 Dec 2021 18:02:32: 3000000 INFO @ Fri, 10 Dec 2021 18:02:38: 7000000 INFO @ Fri, 10 Dec 2021 18:02:38: 9000000 INFO @ Fri, 10 Dec 2021 18:02:43: 4000000 INFO @ Fri, 10 Dec 2021 18:02:48: 8000000 INFO @ Fri, 10 Dec 2021 18:02:49: 10000000 INFO @ Fri, 10 Dec 2021 18:02:55: 5000000 INFO @ Fri, 10 Dec 2021 18:02:58: 9000000 INFO @ Fri, 10 Dec 2021 18:03:00: 11000000 INFO @ Fri, 10 Dec 2021 18:03:07: 6000000 INFO @ Fri, 10 Dec 2021 18:03:08: 10000000 INFO @ Fri, 10 Dec 2021 18:03:12: 12000000 INFO @ Fri, 10 Dec 2021 18:03:19: 11000000 INFO @ Fri, 10 Dec 2021 18:03:19: 7000000 INFO @ Fri, 10 Dec 2021 18:03:23: 13000000 INFO @ Fri, 10 Dec 2021 18:03:29: 12000000 INFO @ Fri, 10 Dec 2021 18:03:30: 8000000 INFO @ Fri, 10 Dec 2021 18:03:35: 14000000 INFO @ Fri, 10 Dec 2021 18:03:39: 13000000 INFO @ Fri, 10 Dec 2021 18:03:42: 9000000 INFO @ Fri, 10 Dec 2021 18:03:46: 15000000 INFO @ Fri, 10 Dec 2021 18:03:50: 14000000 INFO @ Fri, 10 Dec 2021 18:03:54: 10000000 INFO @ Fri, 10 Dec 2021 18:03:57: 16000000 INFO @ Fri, 10 Dec 2021 18:04:00: 15000000 INFO @ Fri, 10 Dec 2021 18:04:06: 11000000 INFO @ Fri, 10 Dec 2021 18:04:08: 17000000 INFO @ Fri, 10 Dec 2021 18:04:10: 16000000 INFO @ Fri, 10 Dec 2021 18:04:17: 12000000 INFO @ Fri, 10 Dec 2021 18:04:20: 18000000 INFO @ Fri, 10 Dec 2021 18:04:21: 17000000 INFO @ Fri, 10 Dec 2021 18:04:29: 13000000 INFO @ Fri, 10 Dec 2021 18:04:31: 19000000 INFO @ Fri, 10 Dec 2021 18:04:31: 18000000 INFO @ Fri, 10 Dec 2021 18:04:41: 14000000 INFO @ Fri, 10 Dec 2021 18:04:42: 19000000 INFO @ Fri, 10 Dec 2021 18:04:43: 20000000 INFO @ Fri, 10 Dec 2021 18:04:53: 15000000 INFO @ Fri, 10 Dec 2021 18:04:53: 20000000 INFO @ Fri, 10 Dec 2021 18:04:54: 21000000 INFO @ Fri, 10 Dec 2021 18:05:04: 21000000 INFO @ Fri, 10 Dec 2021 18:05:05: 16000000 INFO @ Fri, 10 Dec 2021 18:05:06: 22000000 INFO @ Fri, 10 Dec 2021 18:05:15: 22000000 INFO @ Fri, 10 Dec 2021 18:05:17: 17000000 INFO @ Fri, 10 Dec 2021 18:05:17: 23000000 INFO @ Fri, 10 Dec 2021 18:05:26: 23000000 INFO @ Fri, 10 Dec 2021 18:05:29: 24000000 INFO @ Fri, 10 Dec 2021 18:05:29: 18000000 INFO @ Fri, 10 Dec 2021 18:05:36: 24000000 INFO @ Fri, 10 Dec 2021 18:05:40: 25000000 INFO @ Fri, 10 Dec 2021 18:05:41: 19000000 INFO @ Fri, 10 Dec 2021 18:05:47: 25000000 INFO @ Fri, 10 Dec 2021 18:05:52: 26000000 INFO @ Fri, 10 Dec 2021 18:05:53: 20000000 INFO @ Fri, 10 Dec 2021 18:05:57: 26000000 INFO @ Fri, 10 Dec 2021 18:06:04: 27000000 INFO @ Fri, 10 Dec 2021 18:06:04: 21000000 INFO @ Fri, 10 Dec 2021 18:06:08: 27000000 INFO @ Fri, 10 Dec 2021 18:06:16: 28000000 INFO @ Fri, 10 Dec 2021 18:06:16: 22000000 INFO @ Fri, 10 Dec 2021 18:06:18: 28000000 INFO @ Fri, 10 Dec 2021 18:06:27: 29000000 INFO @ Fri, 10 Dec 2021 18:06:28: 23000000 INFO @ Fri, 10 Dec 2021 18:06:29: 29000000 INFO @ Fri, 10 Dec 2021 18:06:39: 30000000 INFO @ Fri, 10 Dec 2021 18:06:40: 24000000 INFO @ Fri, 10 Dec 2021 18:06:40: 30000000 INFO @ Fri, 10 Dec 2021 18:06:51: 31000000 INFO @ Fri, 10 Dec 2021 18:06:51: 31000000 INFO @ Fri, 10 Dec 2021 18:06:52: 25000000 INFO @ Fri, 10 Dec 2021 18:07:00: 32000000 INFO @ Fri, 10 Dec 2021 18:07:03: 32000000 INFO @ Fri, 10 Dec 2021 18:07:04: 26000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 18:07:09: 33000000 INFO @ Fri, 10 Dec 2021 18:07:16: 27000000 INFO @ Fri, 10 Dec 2021 18:07:16: 33000000 INFO @ Fri, 10 Dec 2021 18:07:18: 34000000 INFO @ Fri, 10 Dec 2021 18:07:27: 35000000 INFO @ Fri, 10 Dec 2021 18:07:27: 28000000 INFO @ Fri, 10 Dec 2021 18:07:29: 34000000 INFO @ Fri, 10 Dec 2021 18:07:36: 36000000 INFO @ Fri, 10 Dec 2021 18:07:39: 29000000 INFO @ Fri, 10 Dec 2021 18:07:42: 35000000 INFO @ Fri, 10 Dec 2021 18:07:45: 37000000 INFO @ Fri, 10 Dec 2021 18:07:51: 30000000 INFO @ Fri, 10 Dec 2021 18:07:55: 38000000 INFO @ Fri, 10 Dec 2021 18:07:55: 36000000 INFO @ Fri, 10 Dec 2021 18:08:03: 31000000 INFO @ Fri, 10 Dec 2021 18:08:04: 39000000 INFO @ Fri, 10 Dec 2021 18:08:08: 37000000 INFO @ Fri, 10 Dec 2021 18:08:13: 40000000 INFO @ Fri, 10 Dec 2021 18:08:16: 32000000 INFO @ Fri, 10 Dec 2021 18:08:21: 38000000 INFO @ Fri, 10 Dec 2021 18:08:23: 41000000 INFO @ Fri, 10 Dec 2021 18:08:29: 33000000 INFO @ Fri, 10 Dec 2021 18:08:32: 42000000 INFO @ Fri, 10 Dec 2021 18:08:34: 39000000 INFO @ Fri, 10 Dec 2021 18:08:41: 43000000 INFO @ Fri, 10 Dec 2021 18:08:41: 34000000 INFO @ Fri, 10 Dec 2021 18:08:47: 40000000 INFO @ Fri, 10 Dec 2021 18:08:50: 44000000 INFO @ Fri, 10 Dec 2021 18:08:54: 35000000 INFO @ Fri, 10 Dec 2021 18:09:00: 45000000 INFO @ Fri, 10 Dec 2021 18:09:00: 41000000 INFO @ Fri, 10 Dec 2021 18:09:07: 36000000 INFO @ Fri, 10 Dec 2021 18:09:09: 46000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 18:09:13: 42000000 INFO @ Fri, 10 Dec 2021 18:09:19: 47000000 INFO @ Fri, 10 Dec 2021 18:09:19: 37000000 INFO @ Fri, 10 Dec 2021 18:09:26: 43000000 INFO @ Fri, 10 Dec 2021 18:09:28: 48000000 INFO @ Fri, 10 Dec 2021 18:09:32: 38000000 INFO @ Fri, 10 Dec 2021 18:09:37: 49000000 INFO @ Fri, 10 Dec 2021 18:09:39: 44000000 INFO @ Fri, 10 Dec 2021 18:09:44: 39000000 INFO @ Fri, 10 Dec 2021 18:09:47: 50000000 INFO @ Fri, 10 Dec 2021 18:09:49: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 18:09:49: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 18:09:49: #1 total tags in treatment: 24071686 INFO @ Fri, 10 Dec 2021 18:09:49: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 18:09:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 18:09:49: #1 tags after filtering in treatment: 22106948 INFO @ Fri, 10 Dec 2021 18:09:49: #1 Redundant rate of treatment: 0.08 INFO @ Fri, 10 Dec 2021 18:09:49: #1 finished! INFO @ Fri, 10 Dec 2021 18:09:49: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 18:09:49: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 18:09:51: #2 number of paired peaks: 80 WARNING @ Fri, 10 Dec 2021 18:09:51: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 18:09:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7175252/SRX7175252.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175252/SRX7175252.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175252/SRX7175252.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175252/SRX7175252.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 18:09:52: 45000000 INFO @ Fri, 10 Dec 2021 18:09:57: 40000000 INFO @ Fri, 10 Dec 2021 18:10:05: 46000000 INFO @ Fri, 10 Dec 2021 18:10:09: 41000000 INFO @ Fri, 10 Dec 2021 18:10:17: 47000000 INFO @ Fri, 10 Dec 2021 18:10:22: 42000000 INFO @ Fri, 10 Dec 2021 18:10:30: 48000000 INFO @ Fri, 10 Dec 2021 18:10:34: 43000000 INFO @ Fri, 10 Dec 2021 18:10:43: 49000000 INFO @ Fri, 10 Dec 2021 18:10:47: 44000000 INFO @ Fri, 10 Dec 2021 18:10:55: 50000000 INFO @ Fri, 10 Dec 2021 18:10:58: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 18:10:58: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 18:10:58: #1 total tags in treatment: 24071686 INFO @ Fri, 10 Dec 2021 18:10:58: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 18:10:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 18:10:58: #1 tags after filtering in treatment: 22106948 INFO @ Fri, 10 Dec 2021 18:10:58: #1 Redundant rate of treatment: 0.08 INFO @ Fri, 10 Dec 2021 18:10:58: #1 finished! INFO @ Fri, 10 Dec 2021 18:10:58: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 18:10:58: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 18:11:00: 45000000 INFO @ Fri, 10 Dec 2021 18:11:00: #2 number of paired peaks: 80 WARNING @ Fri, 10 Dec 2021 18:11:00: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 18:11:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7175252/SRX7175252.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175252/SRX7175252.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175252/SRX7175252.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175252/SRX7175252.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 18:11:12: 46000000 INFO @ Fri, 10 Dec 2021 18:11:24: 47000000 INFO @ Fri, 10 Dec 2021 18:11:36: 48000000 INFO @ Fri, 10 Dec 2021 18:11:48: 49000000 INFO @ Fri, 10 Dec 2021 18:12:00: 50000000 INFO @ Fri, 10 Dec 2021 18:12:03: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 18:12:03: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 18:12:03: #1 total tags in treatment: 24071686 INFO @ Fri, 10 Dec 2021 18:12:03: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 18:12:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 18:12:03: #1 tags after filtering in treatment: 22106948 INFO @ Fri, 10 Dec 2021 18:12:03: #1 Redundant rate of treatment: 0.08 INFO @ Fri, 10 Dec 2021 18:12:03: #1 finished! INFO @ Fri, 10 Dec 2021 18:12:03: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 18:12:03: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 18:12:05: #2 number of paired peaks: 80 WARNING @ Fri, 10 Dec 2021 18:12:05: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 18:12:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7175252/SRX7175252.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175252/SRX7175252.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175252/SRX7175252.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175252/SRX7175252.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling