Job ID = 4178653 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 72,270,441 reads read : 144,540,882 reads written : 72,270,441 reads 0-length : 72,270,441 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:55:10 72270441 reads; of these: 72270441 (100.00%) were unpaired; of these: 3975950 (5.50%) aligned 0 times 39638824 (54.85%) aligned exactly 1 time 28655667 (39.65%) aligned >1 times 94.50% overall alignment rate Time searching: 00:55:11 Overall time: 00:55:12 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 16097332 / 68294491 = 0.2357 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Thu, 05 Dec 2019 15:31:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7158310/SRX7158310.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7158310/SRX7158310.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7158310/SRX7158310.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7158310/SRX7158310.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 05 Dec 2019 15:31:00: #1 read tag files... INFO @ Thu, 05 Dec 2019 15:31:00: #1 read treatment tags... INFO @ Thu, 05 Dec 2019 15:31:05: 1000000 INFO @ Thu, 05 Dec 2019 15:31:10: 2000000 INFO @ Thu, 05 Dec 2019 15:31:16: 3000000 INFO @ Thu, 05 Dec 2019 15:31:21: 4000000 INFO @ Thu, 05 Dec 2019 15:31:26: 5000000 INFO @ Thu, 05 Dec 2019 15:31:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7158310/SRX7158310.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7158310/SRX7158310.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7158310/SRX7158310.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7158310/SRX7158310.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 05 Dec 2019 15:31:30: #1 read tag files... INFO @ Thu, 05 Dec 2019 15:31:30: #1 read treatment tags... INFO @ Thu, 05 Dec 2019 15:31:31: 6000000 INFO @ Thu, 05 Dec 2019 15:31:36: 1000000 INFO @ Thu, 05 Dec 2019 15:31:37: 7000000 INFO @ Thu, 05 Dec 2019 15:31:41: 2000000 INFO @ Thu, 05 Dec 2019 15:31:42: 8000000 INFO @ Thu, 05 Dec 2019 15:31:47: 3000000 INFO @ Thu, 05 Dec 2019 15:31:48: 9000000 INFO @ Thu, 05 Dec 2019 15:31:53: 4000000 INFO @ Thu, 05 Dec 2019 15:31:53: 10000000 BedGraph に変換中... INFO @ Thu, 05 Dec 2019 15:31:58: 5000000 INFO @ Thu, 05 Dec 2019 15:31:58: 11000000 INFO @ Thu, 05 Dec 2019 15:32:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7158310/SRX7158310.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7158310/SRX7158310.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7158310/SRX7158310.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7158310/SRX7158310.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 05 Dec 2019 15:32:00: #1 read tag files... INFO @ Thu, 05 Dec 2019 15:32:00: #1 read treatment tags... INFO @ Thu, 05 Dec 2019 15:32:04: 12000000 INFO @ Thu, 05 Dec 2019 15:32:04: 6000000 INFO @ Thu, 05 Dec 2019 15:32:06: 1000000 INFO @ Thu, 05 Dec 2019 15:32:10: 7000000 INFO @ Thu, 05 Dec 2019 15:32:10: 13000000 INFO @ Thu, 05 Dec 2019 15:32:11: 2000000 INFO @ Thu, 05 Dec 2019 15:32:15: 14000000 INFO @ Thu, 05 Dec 2019 15:32:15: 8000000 INFO @ Thu, 05 Dec 2019 15:32:17: 3000000 INFO @ Thu, 05 Dec 2019 15:32:21: 15000000 INFO @ Thu, 05 Dec 2019 15:32:21: 9000000 INFO @ Thu, 05 Dec 2019 15:32:22: 4000000 INFO @ Thu, 05 Dec 2019 15:32:26: 16000000 INFO @ Thu, 05 Dec 2019 15:32:26: 10000000 INFO @ Thu, 05 Dec 2019 15:32:28: 5000000 INFO @ Thu, 05 Dec 2019 15:32:32: 17000000 INFO @ Thu, 05 Dec 2019 15:32:32: 11000000 INFO @ Thu, 05 Dec 2019 15:32:33: 6000000 INFO @ Thu, 05 Dec 2019 15:32:37: 18000000 INFO @ Thu, 05 Dec 2019 15:32:37: 12000000 INFO @ Thu, 05 Dec 2019 15:32:39: 7000000 INFO @ Thu, 05 Dec 2019 15:32:43: 19000000 INFO @ Thu, 05 Dec 2019 15:32:43: 13000000 INFO @ Thu, 05 Dec 2019 15:32:45: 8000000 INFO @ Thu, 05 Dec 2019 15:32:48: 20000000 INFO @ Thu, 05 Dec 2019 15:32:49: 14000000 INFO @ Thu, 05 Dec 2019 15:32:50: 9000000 INFO @ Thu, 05 Dec 2019 15:32:54: 21000000 INFO @ Thu, 05 Dec 2019 15:32:54: 15000000 INFO @ Thu, 05 Dec 2019 15:32:56: 10000000 INFO @ Thu, 05 Dec 2019 15:32:59: 22000000 INFO @ Thu, 05 Dec 2019 15:33:00: 16000000 INFO @ Thu, 05 Dec 2019 15:33:01: 11000000 INFO @ Thu, 05 Dec 2019 15:33:05: 23000000 INFO @ Thu, 05 Dec 2019 15:33:06: 17000000 INFO @ Thu, 05 Dec 2019 15:33:07: 12000000 INFO @ Thu, 05 Dec 2019 15:33:10: 24000000 INFO @ Thu, 05 Dec 2019 15:33:11: 18000000 INFO @ Thu, 05 Dec 2019 15:33:13: 13000000 INFO @ Thu, 05 Dec 2019 15:33:16: 25000000 INFO @ Thu, 05 Dec 2019 15:33:17: 19000000 INFO @ Thu, 05 Dec 2019 15:33:18: 14000000 INFO @ Thu, 05 Dec 2019 15:33:22: 26000000 INFO @ Thu, 05 Dec 2019 15:33:23: 20000000 INFO @ Thu, 05 Dec 2019 15:33:24: 15000000 INFO @ Thu, 05 Dec 2019 15:33:28: 27000000 INFO @ Thu, 05 Dec 2019 15:33:28: 21000000 INFO @ Thu, 05 Dec 2019 15:33:30: 16000000 INFO @ Thu, 05 Dec 2019 15:33:34: 22000000 INFO @ Thu, 05 Dec 2019 15:33:34: 28000000 INFO @ Thu, 05 Dec 2019 15:33:35: 17000000 INFO @ Thu, 05 Dec 2019 15:33:39: 23000000 INFO @ Thu, 05 Dec 2019 15:33:40: 29000000 INFO @ Thu, 05 Dec 2019 15:33:41: 18000000 INFO @ Thu, 05 Dec 2019 15:33:45: 24000000 INFO @ Thu, 05 Dec 2019 15:33:45: 30000000 INFO @ Thu, 05 Dec 2019 15:33:47: 19000000 INFO @ Thu, 05 Dec 2019 15:33:51: 25000000 INFO @ Thu, 05 Dec 2019 15:33:51: 31000000 INFO @ Thu, 05 Dec 2019 15:33:53: 20000000 INFO @ Thu, 05 Dec 2019 15:33:56: 26000000 INFO @ Thu, 05 Dec 2019 15:33:57: 32000000 INFO @ Thu, 05 Dec 2019 15:33:58: 21000000 INFO @ Thu, 05 Dec 2019 15:34:02: 27000000 INFO @ Thu, 05 Dec 2019 15:34:02: 33000000 INFO @ Thu, 05 Dec 2019 15:34:04: 22000000 INFO @ Thu, 05 Dec 2019 15:34:08: 28000000 INFO @ Thu, 05 Dec 2019 15:34:08: 34000000 INFO @ Thu, 05 Dec 2019 15:34:10: 23000000 INFO @ Thu, 05 Dec 2019 15:34:14: 29000000 INFO @ Thu, 05 Dec 2019 15:34:14: 35000000 INFO @ Thu, 05 Dec 2019 15:34:16: 24000000 INFO @ Thu, 05 Dec 2019 15:34:19: 30000000 INFO @ Thu, 05 Dec 2019 15:34:20: 36000000 INFO @ Thu, 05 Dec 2019 15:34:22: 25000000 INFO @ Thu, 05 Dec 2019 15:34:25: 31000000 INFO @ Thu, 05 Dec 2019 15:34:25: 37000000 INFO @ Thu, 05 Dec 2019 15:34:28: 26000000 INFO @ Thu, 05 Dec 2019 15:34:31: 32000000 INFO @ Thu, 05 Dec 2019 15:34:31: 38000000 INFO @ Thu, 05 Dec 2019 15:34:33: 27000000 INFO @ Thu, 05 Dec 2019 15:34:36: 33000000 INFO @ Thu, 05 Dec 2019 15:34:37: 39000000 INFO @ Thu, 05 Dec 2019 15:34:39: 28000000 INFO @ Thu, 05 Dec 2019 15:34:42: 34000000 INFO @ Thu, 05 Dec 2019 15:34:42: 40000000 INFO @ Thu, 05 Dec 2019 15:34:45: 29000000 INFO @ Thu, 05 Dec 2019 15:34:48: 35000000 INFO @ Thu, 05 Dec 2019 15:34:48: 41000000 INFO @ Thu, 05 Dec 2019 15:34:50: 30000000 INFO @ Thu, 05 Dec 2019 15:34:54: 36000000 INFO @ Thu, 05 Dec 2019 15:34:54: 42000000 INFO @ Thu, 05 Dec 2019 15:34:56: 31000000 INFO @ Thu, 05 Dec 2019 15:34:59: 43000000 INFO @ Thu, 05 Dec 2019 15:34:59: 37000000 INFO @ Thu, 05 Dec 2019 15:35:02: 32000000 INFO @ Thu, 05 Dec 2019 15:35:05: 44000000 INFO @ Thu, 05 Dec 2019 15:35:05: 38000000 INFO @ Thu, 05 Dec 2019 15:35:07: 33000000 INFO @ Thu, 05 Dec 2019 15:35:10: 45000000 INFO @ Thu, 05 Dec 2019 15:35:11: 39000000 INFO @ Thu, 05 Dec 2019 15:35:13: 34000000 INFO @ Thu, 05 Dec 2019 15:35:16: 46000000 INFO @ Thu, 05 Dec 2019 15:35:16: 40000000 INFO @ Thu, 05 Dec 2019 15:35:19: 35000000 INFO @ Thu, 05 Dec 2019 15:35:22: 47000000 INFO @ Thu, 05 Dec 2019 15:35:22: 41000000 INFO @ Thu, 05 Dec 2019 15:35:25: 36000000 INFO @ Thu, 05 Dec 2019 15:35:28: 48000000 INFO @ Thu, 05 Dec 2019 15:35:28: 42000000 INFO @ Thu, 05 Dec 2019 15:35:31: 37000000 INFO @ Thu, 05 Dec 2019 15:35:33: 43000000 INFO @ Thu, 05 Dec 2019 15:35:33: 49000000 INFO @ Thu, 05 Dec 2019 15:35:37: 38000000 INFO @ Thu, 05 Dec 2019 15:35:39: 44000000 INFO @ Thu, 05 Dec 2019 15:35:39: 50000000 INFO @ Thu, 05 Dec 2019 15:35:43: 39000000 INFO @ Thu, 05 Dec 2019 15:35:44: 45000000 INFO @ Thu, 05 Dec 2019 15:35:45: 51000000 INFO @ Thu, 05 Dec 2019 15:35:48: 40000000 INFO @ Thu, 05 Dec 2019 15:35:50: 46000000 INFO @ Thu, 05 Dec 2019 15:35:50: 52000000 INFO @ Thu, 05 Dec 2019 15:35:52: #1 tag size is determined as 75 bps INFO @ Thu, 05 Dec 2019 15:35:52: #1 tag size = 75 INFO @ Thu, 05 Dec 2019 15:35:52: #1 total tags in treatment: 52197159 INFO @ Thu, 05 Dec 2019 15:35:52: #1 user defined the maximum tags... INFO @ Thu, 05 Dec 2019 15:35:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 05 Dec 2019 15:35:53: #1 tags after filtering in treatment: 52197159 INFO @ Thu, 05 Dec 2019 15:35:53: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 05 Dec 2019 15:35:53: #1 finished! INFO @ Thu, 05 Dec 2019 15:35:53: #2 Build Peak Model... INFO @ Thu, 05 Dec 2019 15:35:53: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 05 Dec 2019 15:35:55: 41000000 INFO @ Thu, 05 Dec 2019 15:35:55: 47000000 INFO @ Thu, 05 Dec 2019 15:35:56: #2 number of paired peaks: 0 WARNING @ Thu, 05 Dec 2019 15:35:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 05 Dec 2019 15:35:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7158310/SRX7158310.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7158310/SRX7158310.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7158310/SRX7158310.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7158310/SRX7158310.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 05 Dec 2019 15:36:00: 48000000 INFO @ Thu, 05 Dec 2019 15:36:01: 42000000 INFO @ Thu, 05 Dec 2019 15:36:06: 49000000 INFO @ Thu, 05 Dec 2019 15:36:08: 43000000 INFO @ Thu, 05 Dec 2019 15:36:11: 50000000 INFO @ Thu, 05 Dec 2019 15:36:14: 44000000 INFO @ Thu, 05 Dec 2019 15:36:16: 51000000 INFO @ Thu, 05 Dec 2019 15:36:20: 45000000 INFO @ Thu, 05 Dec 2019 15:36:21: 52000000 INFO @ Thu, 05 Dec 2019 15:36:23: #1 tag size is determined as 75 bps INFO @ Thu, 05 Dec 2019 15:36:23: #1 tag size = 75 INFO @ Thu, 05 Dec 2019 15:36:23: #1 total tags in treatment: 52197159 INFO @ Thu, 05 Dec 2019 15:36:23: #1 user defined the maximum tags... INFO @ Thu, 05 Dec 2019 15:36:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 05 Dec 2019 15:36:24: #1 tags after filtering in treatment: 52197159 INFO @ Thu, 05 Dec 2019 15:36:24: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 05 Dec 2019 15:36:24: #1 finished! INFO @ Thu, 05 Dec 2019 15:36:24: #2 Build Peak Model... INFO @ Thu, 05 Dec 2019 15:36:24: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 05 Dec 2019 15:36:27: 46000000 INFO @ Thu, 05 Dec 2019 15:36:27: #2 number of paired peaks: 0 WARNING @ Thu, 05 Dec 2019 15:36:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 05 Dec 2019 15:36:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7158310/SRX7158310.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7158310/SRX7158310.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7158310/SRX7158310.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7158310/SRX7158310.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 05 Dec 2019 15:36:33: 47000000 INFO @ Thu, 05 Dec 2019 15:36:40: 48000000 INFO @ Thu, 05 Dec 2019 15:36:46: 49000000 INFO @ Thu, 05 Dec 2019 15:36:53: 50000000 INFO @ Thu, 05 Dec 2019 15:37:00: 51000000 INFO @ Thu, 05 Dec 2019 15:37:07: 52000000 INFO @ Thu, 05 Dec 2019 15:37:08: #1 tag size is determined as 75 bps INFO @ Thu, 05 Dec 2019 15:37:08: #1 tag size = 75 INFO @ Thu, 05 Dec 2019 15:37:08: #1 total tags in treatment: 52197159 INFO @ Thu, 05 Dec 2019 15:37:08: #1 user defined the maximum tags... INFO @ Thu, 05 Dec 2019 15:37:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 05 Dec 2019 15:37:09: #1 tags after filtering in treatment: 52197159 INFO @ Thu, 05 Dec 2019 15:37:09: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 05 Dec 2019 15:37:09: #1 finished! INFO @ Thu, 05 Dec 2019 15:37:09: #2 Build Peak Model... INFO @ Thu, 05 Dec 2019 15:37:09: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 05 Dec 2019 15:37:12: #2 number of paired peaks: 0 WARNING @ Thu, 05 Dec 2019 15:37:12: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 05 Dec 2019 15:37:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7158310/SRX7158310.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7158310/SRX7158310.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7158310/SRX7158310.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7158310/SRX7158310.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。