Job ID = 4178651 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 75,551,897 reads read : 151,103,794 reads written : 75,551,897 reads 0-length : 75,551,897 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:29:00 75551897 reads; of these: 75551897 (100.00%) were unpaired; of these: 2146600 (2.84%) aligned 0 times 63251980 (83.72%) aligned exactly 1 time 10153317 (13.44%) aligned >1 times 97.16% overall alignment rate Time searching: 00:29:01 Overall time: 00:29:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 32 files... [bam_rmdupse_core] 14926740 / 73405297 = 0.2033 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Thu, 05 Dec 2019 14:58:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7158309/SRX7158309.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7158309/SRX7158309.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7158309/SRX7158309.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7158309/SRX7158309.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 05 Dec 2019 14:58:55: #1 read tag files... INFO @ Thu, 05 Dec 2019 14:58:55: #1 read treatment tags... INFO @ Thu, 05 Dec 2019 14:59:02: 1000000 INFO @ Thu, 05 Dec 2019 14:59:08: 2000000 INFO @ Thu, 05 Dec 2019 14:59:14: 3000000 INFO @ Thu, 05 Dec 2019 14:59:21: 4000000 INFO @ Thu, 05 Dec 2019 14:59:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7158309/SRX7158309.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7158309/SRX7158309.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7158309/SRX7158309.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7158309/SRX7158309.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 05 Dec 2019 14:59:24: #1 read tag files... INFO @ Thu, 05 Dec 2019 14:59:24: #1 read treatment tags... INFO @ Thu, 05 Dec 2019 14:59:27: 5000000 INFO @ Thu, 05 Dec 2019 14:59:31: 1000000 INFO @ Thu, 05 Dec 2019 14:59:34: 6000000 INFO @ Thu, 05 Dec 2019 14:59:37: 2000000 INFO @ Thu, 05 Dec 2019 14:59:41: 7000000 INFO @ Thu, 05 Dec 2019 14:59:43: 3000000 INFO @ Thu, 05 Dec 2019 14:59:47: 8000000 INFO @ Thu, 05 Dec 2019 14:59:49: 4000000 BedGraph に変換中... INFO @ Thu, 05 Dec 2019 14:59:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7158309/SRX7158309.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7158309/SRX7158309.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7158309/SRX7158309.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7158309/SRX7158309.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 05 Dec 2019 14:59:54: #1 read tag files... INFO @ Thu, 05 Dec 2019 14:59:54: #1 read treatment tags... INFO @ Thu, 05 Dec 2019 14:59:54: 9000000 INFO @ Thu, 05 Dec 2019 14:59:55: 5000000 INFO @ Thu, 05 Dec 2019 15:00:01: 1000000 INFO @ Thu, 05 Dec 2019 15:00:01: 10000000 INFO @ Thu, 05 Dec 2019 15:00:02: 6000000 INFO @ Thu, 05 Dec 2019 15:00:07: 2000000 INFO @ Thu, 05 Dec 2019 15:00:08: 11000000 INFO @ Thu, 05 Dec 2019 15:00:09: 7000000 INFO @ Thu, 05 Dec 2019 15:00:14: 3000000 INFO @ Thu, 05 Dec 2019 15:00:15: 12000000 INFO @ Thu, 05 Dec 2019 15:00:15: 8000000 INFO @ Thu, 05 Dec 2019 15:00:20: 4000000 INFO @ Thu, 05 Dec 2019 15:00:21: 13000000 INFO @ Thu, 05 Dec 2019 15:00:22: 9000000 INFO @ Thu, 05 Dec 2019 15:00:27: 5000000 INFO @ Thu, 05 Dec 2019 15:00:28: 14000000 INFO @ Thu, 05 Dec 2019 15:00:28: 10000000 INFO @ Thu, 05 Dec 2019 15:00:34: 6000000 INFO @ Thu, 05 Dec 2019 15:00:35: 11000000 INFO @ Thu, 05 Dec 2019 15:00:35: 15000000 INFO @ Thu, 05 Dec 2019 15:00:40: 7000000 INFO @ Thu, 05 Dec 2019 15:00:41: 12000000 INFO @ Thu, 05 Dec 2019 15:00:41: 16000000 INFO @ Thu, 05 Dec 2019 15:00:47: 8000000 INFO @ Thu, 05 Dec 2019 15:00:48: 13000000 INFO @ Thu, 05 Dec 2019 15:00:48: 17000000 INFO @ Thu, 05 Dec 2019 15:00:53: 9000000 INFO @ Thu, 05 Dec 2019 15:00:54: 14000000 INFO @ Thu, 05 Dec 2019 15:00:55: 18000000 INFO @ Thu, 05 Dec 2019 15:01:00: 10000000 INFO @ Thu, 05 Dec 2019 15:01:00: 15000000 INFO @ Thu, 05 Dec 2019 15:01:01: 19000000 INFO @ Thu, 05 Dec 2019 15:01:06: 11000000 INFO @ Thu, 05 Dec 2019 15:01:07: 16000000 INFO @ Thu, 05 Dec 2019 15:01:08: 20000000 INFO @ Thu, 05 Dec 2019 15:01:13: 12000000 INFO @ Thu, 05 Dec 2019 15:01:14: 17000000 INFO @ Thu, 05 Dec 2019 15:01:15: 21000000 INFO @ Thu, 05 Dec 2019 15:01:19: 13000000 INFO @ Thu, 05 Dec 2019 15:01:20: 18000000 INFO @ Thu, 05 Dec 2019 15:01:21: 22000000 INFO @ Thu, 05 Dec 2019 15:01:26: 14000000 INFO @ Thu, 05 Dec 2019 15:01:27: 19000000 INFO @ Thu, 05 Dec 2019 15:01:28: 23000000 INFO @ Thu, 05 Dec 2019 15:01:32: 15000000 INFO @ Thu, 05 Dec 2019 15:01:33: 20000000 INFO @ Thu, 05 Dec 2019 15:01:35: 24000000 INFO @ Thu, 05 Dec 2019 15:01:38: 16000000 INFO @ Thu, 05 Dec 2019 15:01:40: 21000000 INFO @ Thu, 05 Dec 2019 15:01:41: 25000000 INFO @ Thu, 05 Dec 2019 15:01:45: 17000000 INFO @ Thu, 05 Dec 2019 15:01:46: 22000000 INFO @ Thu, 05 Dec 2019 15:01:48: 26000000 INFO @ Thu, 05 Dec 2019 15:01:52: 18000000 INFO @ Thu, 05 Dec 2019 15:01:53: 23000000 INFO @ Thu, 05 Dec 2019 15:01:54: 27000000 INFO @ Thu, 05 Dec 2019 15:01:58: 19000000 INFO @ Thu, 05 Dec 2019 15:02:00: 24000000 INFO @ Thu, 05 Dec 2019 15:02:01: 28000000 INFO @ Thu, 05 Dec 2019 15:02:04: 20000000 INFO @ Thu, 05 Dec 2019 15:02:07: 25000000 INFO @ Thu, 05 Dec 2019 15:02:08: 29000000 INFO @ Thu, 05 Dec 2019 15:02:11: 21000000 INFO @ Thu, 05 Dec 2019 15:02:13: 26000000 INFO @ Thu, 05 Dec 2019 15:02:14: 30000000 INFO @ Thu, 05 Dec 2019 15:02:17: 22000000 INFO @ Thu, 05 Dec 2019 15:02:20: 27000000 INFO @ Thu, 05 Dec 2019 15:02:21: 31000000 INFO @ Thu, 05 Dec 2019 15:02:23: 23000000 INFO @ Thu, 05 Dec 2019 15:02:27: 28000000 INFO @ Thu, 05 Dec 2019 15:02:28: 32000000 INFO @ Thu, 05 Dec 2019 15:02:30: 24000000 INFO @ Thu, 05 Dec 2019 15:02:33: 29000000 INFO @ Thu, 05 Dec 2019 15:02:34: 33000000 INFO @ Thu, 05 Dec 2019 15:02:36: 25000000 INFO @ Thu, 05 Dec 2019 15:02:40: 30000000 INFO @ Thu, 05 Dec 2019 15:02:41: 34000000 INFO @ Thu, 05 Dec 2019 15:02:43: 26000000 INFO @ Thu, 05 Dec 2019 15:02:46: 31000000 INFO @ Thu, 05 Dec 2019 15:02:47: 35000000 INFO @ Thu, 05 Dec 2019 15:02:49: 27000000 INFO @ Thu, 05 Dec 2019 15:02:53: 32000000 INFO @ Thu, 05 Dec 2019 15:02:54: 36000000 INFO @ Thu, 05 Dec 2019 15:02:56: 28000000 INFO @ Thu, 05 Dec 2019 15:02:59: 33000000 INFO @ Thu, 05 Dec 2019 15:03:00: 37000000 INFO @ Thu, 05 Dec 2019 15:03:02: 29000000 INFO @ Thu, 05 Dec 2019 15:03:06: 34000000 INFO @ Thu, 05 Dec 2019 15:03:07: 38000000 INFO @ Thu, 05 Dec 2019 15:03:09: 30000000 INFO @ Thu, 05 Dec 2019 15:03:13: 35000000 INFO @ Thu, 05 Dec 2019 15:03:13: 39000000 INFO @ Thu, 05 Dec 2019 15:03:16: 31000000 INFO @ Thu, 05 Dec 2019 15:03:19: 36000000 INFO @ Thu, 05 Dec 2019 15:03:20: 40000000 INFO @ Thu, 05 Dec 2019 15:03:22: 32000000 INFO @ Thu, 05 Dec 2019 15:03:26: 37000000 INFO @ Thu, 05 Dec 2019 15:03:26: 41000000 INFO @ Thu, 05 Dec 2019 15:03:29: 33000000 INFO @ Thu, 05 Dec 2019 15:03:32: 38000000 INFO @ Thu, 05 Dec 2019 15:03:33: 42000000 INFO @ Thu, 05 Dec 2019 15:03:35: 34000000 INFO @ Thu, 05 Dec 2019 15:03:39: 39000000 INFO @ Thu, 05 Dec 2019 15:03:39: 43000000 INFO @ Thu, 05 Dec 2019 15:03:42: 35000000 INFO @ Thu, 05 Dec 2019 15:03:45: 40000000 INFO @ Thu, 05 Dec 2019 15:03:46: 44000000 INFO @ Thu, 05 Dec 2019 15:03:49: 36000000 INFO @ Thu, 05 Dec 2019 15:03:52: 41000000 INFO @ Thu, 05 Dec 2019 15:03:52: 45000000 INFO @ Thu, 05 Dec 2019 15:03:55: 37000000 INFO @ Thu, 05 Dec 2019 15:03:59: 42000000 INFO @ Thu, 05 Dec 2019 15:03:59: 46000000 INFO @ Thu, 05 Dec 2019 15:04:02: 38000000 INFO @ Thu, 05 Dec 2019 15:04:05: 43000000 INFO @ Thu, 05 Dec 2019 15:04:05: 47000000 INFO @ Thu, 05 Dec 2019 15:04:08: 39000000 INFO @ Thu, 05 Dec 2019 15:04:12: 48000000 INFO @ Thu, 05 Dec 2019 15:04:12: 44000000 INFO @ Thu, 05 Dec 2019 15:04:15: 40000000 INFO @ Thu, 05 Dec 2019 15:04:18: 49000000 INFO @ Thu, 05 Dec 2019 15:04:19: 45000000 INFO @ Thu, 05 Dec 2019 15:04:22: 41000000 INFO @ Thu, 05 Dec 2019 15:04:24: 50000000 INFO @ Thu, 05 Dec 2019 15:04:25: 46000000 INFO @ Thu, 05 Dec 2019 15:04:28: 42000000 INFO @ Thu, 05 Dec 2019 15:04:31: 51000000 INFO @ Thu, 05 Dec 2019 15:04:32: 47000000 INFO @ Thu, 05 Dec 2019 15:04:35: 43000000 INFO @ Thu, 05 Dec 2019 15:04:38: 52000000 INFO @ Thu, 05 Dec 2019 15:04:39: 48000000 INFO @ Thu, 05 Dec 2019 15:04:41: 44000000 INFO @ Thu, 05 Dec 2019 15:04:44: 53000000 INFO @ Thu, 05 Dec 2019 15:04:45: 49000000 INFO @ Thu, 05 Dec 2019 15:04:48: 45000000 INFO @ Thu, 05 Dec 2019 15:04:51: 54000000 INFO @ Thu, 05 Dec 2019 15:04:52: 50000000 INFO @ Thu, 05 Dec 2019 15:04:54: 46000000 INFO @ Thu, 05 Dec 2019 15:04:57: 55000000 INFO @ Thu, 05 Dec 2019 15:04:58: 51000000 INFO @ Thu, 05 Dec 2019 15:05:01: 47000000 INFO @ Thu, 05 Dec 2019 15:05:04: 56000000 INFO @ Thu, 05 Dec 2019 15:05:05: 52000000 INFO @ Thu, 05 Dec 2019 15:05:08: 48000000 INFO @ Thu, 05 Dec 2019 15:05:10: 57000000 INFO @ Thu, 05 Dec 2019 15:05:11: 53000000 INFO @ Thu, 05 Dec 2019 15:05:14: 49000000 INFO @ Thu, 05 Dec 2019 15:05:17: 58000000 INFO @ Thu, 05 Dec 2019 15:05:18: 54000000 INFO @ Thu, 05 Dec 2019 15:05:21: #1 tag size is determined as 75 bps INFO @ Thu, 05 Dec 2019 15:05:21: #1 tag size = 75 INFO @ Thu, 05 Dec 2019 15:05:21: #1 total tags in treatment: 58478557 INFO @ Thu, 05 Dec 2019 15:05:21: #1 user defined the maximum tags... INFO @ Thu, 05 Dec 2019 15:05:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 05 Dec 2019 15:05:21: 50000000 INFO @ Thu, 05 Dec 2019 15:05:22: #1 tags after filtering in treatment: 58478557 INFO @ Thu, 05 Dec 2019 15:05:22: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 05 Dec 2019 15:05:22: #1 finished! INFO @ Thu, 05 Dec 2019 15:05:22: #2 Build Peak Model... INFO @ Thu, 05 Dec 2019 15:05:22: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 05 Dec 2019 15:05:24: 55000000 INFO @ Thu, 05 Dec 2019 15:05:25: #2 number of paired peaks: 0 WARNING @ Thu, 05 Dec 2019 15:05:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 05 Dec 2019 15:05:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7158309/SRX7158309.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis INFO @ Thu, 05 Dec 2019 15:05:27: 51000000 needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7158309/SRX7158309.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7158309/SRX7158309.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7158309/SRX7158309.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 05 Dec 2019 15:05:31: 56000000 INFO @ Thu, 05 Dec 2019 15:05:34: 52000000 INFO @ Thu, 05 Dec 2019 15:05:37: 57000000 INFO @ Thu, 05 Dec 2019 15:05:40: 53000000 INFO @ Thu, 05 Dec 2019 15:05:44: 58000000 INFO @ Thu, 05 Dec 2019 15:05:46: 54000000 INFO @ Thu, 05 Dec 2019 15:05:47: #1 tag size is determined as 75 bps INFO @ Thu, 05 Dec 2019 15:05:47: #1 tag size = 75 INFO @ Thu, 05 Dec 2019 15:05:47: #1 total tags in treatment: 58478557 INFO @ Thu, 05 Dec 2019 15:05:47: #1 user defined the maximum tags... INFO @ Thu, 05 Dec 2019 15:05:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 05 Dec 2019 15:05:48: #1 tags after filtering in treatment: 58478557 INFO @ Thu, 05 Dec 2019 15:05:48: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 05 Dec 2019 15:05:48: #1 finished! INFO @ Thu, 05 Dec 2019 15:05:48: #2 Build Peak Model... INFO @ Thu, 05 Dec 2019 15:05:48: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 05 Dec 2019 15:05:52: #2 number of paired peaks: 0 WARNING @ Thu, 05 Dec 2019 15:05:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 05 Dec 2019 15:05:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7158309/SRX7158309.10_peaks.narrowPeak: No such file or directory INFO @ Thu, 05 Dec 2019 15:05:52: 55000000 pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7158309/SRX7158309.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7158309/SRX7158309.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7158309/SRX7158309.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 05 Dec 2019 15:05:58: 56000000 INFO @ Thu, 05 Dec 2019 15:06:04: 57000000 INFO @ Thu, 05 Dec 2019 15:06:09: 58000000 INFO @ Thu, 05 Dec 2019 15:06:13: #1 tag size is determined as 75 bps INFO @ Thu, 05 Dec 2019 15:06:13: #1 tag size = 75 INFO @ Thu, 05 Dec 2019 15:06:13: #1 total tags in treatment: 58478557 INFO @ Thu, 05 Dec 2019 15:06:13: #1 user defined the maximum tags... INFO @ Thu, 05 Dec 2019 15:06:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 05 Dec 2019 15:06:14: #1 tags after filtering in treatment: 58478557 INFO @ Thu, 05 Dec 2019 15:06:14: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 05 Dec 2019 15:06:14: #1 finished! INFO @ Thu, 05 Dec 2019 15:06:14: #2 Build Peak Model... INFO @ Thu, 05 Dec 2019 15:06:14: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 05 Dec 2019 15:06:17: #2 number of paired peaks: 0 WARNING @ Thu, 05 Dec 2019 15:06:17: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 05 Dec 2019 15:06:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7158309/SRX7158309.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7158309/SRX7158309.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7158309/SRX7158309.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7158309/SRX7158309.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。