Job ID = 6528474 SRX = SRX706809 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T15:16:36 prefetch.2.10.7: 1) Downloading 'SRR1581490'... 2020-06-29T15:16:36 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:19:36 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:19:36 prefetch.2.10.7: 1) 'SRR1581490' was downloaded successfully Read 23512132 spots for SRR1581490/SRR1581490.sra Written 23512132 spots for SRR1581490/SRR1581490.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:11 23512132 reads; of these: 23512132 (100.00%) were unpaired; of these: 1205196 (5.13%) aligned 0 times 19792763 (84.18%) aligned exactly 1 time 2514173 (10.69%) aligned >1 times 94.87% overall alignment rate Time searching: 00:05:11 Overall time: 00:05:11 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2988640 / 22306936 = 0.1340 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:37:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX706809/SRX706809.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX706809/SRX706809.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX706809/SRX706809.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX706809/SRX706809.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:37:03: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:37:03: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:37:10: 1000000 INFO @ Tue, 30 Jun 2020 00:37:16: 2000000 INFO @ Tue, 30 Jun 2020 00:37:22: 3000000 INFO @ Tue, 30 Jun 2020 00:37:29: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:37:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX706809/SRX706809.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX706809/SRX706809.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX706809/SRX706809.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX706809/SRX706809.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:37:34: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:37:34: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:37:35: 5000000 INFO @ Tue, 30 Jun 2020 00:37:41: 1000000 INFO @ Tue, 30 Jun 2020 00:37:42: 6000000 INFO @ Tue, 30 Jun 2020 00:37:48: 2000000 INFO @ Tue, 30 Jun 2020 00:37:49: 7000000 INFO @ Tue, 30 Jun 2020 00:37:54: 3000000 INFO @ Tue, 30 Jun 2020 00:37:56: 8000000 INFO @ Tue, 30 Jun 2020 00:38:01: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:38:03: 9000000 INFO @ Tue, 30 Jun 2020 00:38:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX706809/SRX706809.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX706809/SRX706809.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX706809/SRX706809.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX706809/SRX706809.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:38:03: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:38:03: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:38:08: 5000000 INFO @ Tue, 30 Jun 2020 00:38:10: 10000000 INFO @ Tue, 30 Jun 2020 00:38:10: 1000000 INFO @ Tue, 30 Jun 2020 00:38:15: 6000000 INFO @ Tue, 30 Jun 2020 00:38:17: 11000000 INFO @ Tue, 30 Jun 2020 00:38:17: 2000000 INFO @ Tue, 30 Jun 2020 00:38:22: 7000000 INFO @ Tue, 30 Jun 2020 00:38:23: 12000000 INFO @ Tue, 30 Jun 2020 00:38:24: 3000000 INFO @ Tue, 30 Jun 2020 00:38:28: 8000000 INFO @ Tue, 30 Jun 2020 00:38:30: 13000000 INFO @ Tue, 30 Jun 2020 00:38:31: 4000000 INFO @ Tue, 30 Jun 2020 00:38:35: 9000000 INFO @ Tue, 30 Jun 2020 00:38:37: 14000000 INFO @ Tue, 30 Jun 2020 00:38:38: 5000000 INFO @ Tue, 30 Jun 2020 00:38:42: 10000000 INFO @ Tue, 30 Jun 2020 00:38:44: 15000000 INFO @ Tue, 30 Jun 2020 00:38:45: 6000000 INFO @ Tue, 30 Jun 2020 00:38:48: 11000000 INFO @ Tue, 30 Jun 2020 00:38:51: 16000000 INFO @ Tue, 30 Jun 2020 00:38:52: 7000000 INFO @ Tue, 30 Jun 2020 00:38:55: 12000000 INFO @ Tue, 30 Jun 2020 00:38:58: 17000000 INFO @ Tue, 30 Jun 2020 00:38:59: 8000000 INFO @ Tue, 30 Jun 2020 00:39:01: 13000000 INFO @ Tue, 30 Jun 2020 00:39:05: 18000000 INFO @ Tue, 30 Jun 2020 00:39:06: 9000000 INFO @ Tue, 30 Jun 2020 00:39:07: 14000000 INFO @ Tue, 30 Jun 2020 00:39:12: 19000000 INFO @ Tue, 30 Jun 2020 00:39:13: 10000000 INFO @ Tue, 30 Jun 2020 00:39:14: 15000000 INFO @ Tue, 30 Jun 2020 00:39:14: #1 tag size is determined as 44 bps INFO @ Tue, 30 Jun 2020 00:39:14: #1 tag size = 44 INFO @ Tue, 30 Jun 2020 00:39:14: #1 total tags in treatment: 19318296 INFO @ Tue, 30 Jun 2020 00:39:14: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:39:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:39:14: #1 tags after filtering in treatment: 19318296 INFO @ Tue, 30 Jun 2020 00:39:14: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:39:14: #1 finished! INFO @ Tue, 30 Jun 2020 00:39:14: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:39:14: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:39:16: #2 number of paired peaks: 86 WARNING @ Tue, 30 Jun 2020 00:39:16: Too few paired peaks (86) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:39:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX706809/SRX706809.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX706809/SRX706809.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX706809/SRX706809.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX706809/SRX706809.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:39:20: 11000000 INFO @ Tue, 30 Jun 2020 00:39:20: 16000000 INFO @ Tue, 30 Jun 2020 00:39:26: 17000000 INFO @ Tue, 30 Jun 2020 00:39:27: 12000000 INFO @ Tue, 30 Jun 2020 00:39:32: 18000000 INFO @ Tue, 30 Jun 2020 00:39:34: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:39:39: 19000000 INFO @ Tue, 30 Jun 2020 00:39:40: 14000000 INFO @ Tue, 30 Jun 2020 00:39:41: #1 tag size is determined as 44 bps INFO @ Tue, 30 Jun 2020 00:39:41: #1 tag size = 44 INFO @ Tue, 30 Jun 2020 00:39:41: #1 total tags in treatment: 19318296 INFO @ Tue, 30 Jun 2020 00:39:41: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:39:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:39:41: #1 tags after filtering in treatment: 19318296 INFO @ Tue, 30 Jun 2020 00:39:41: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:39:41: #1 finished! INFO @ Tue, 30 Jun 2020 00:39:41: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:39:41: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:39:43: #2 number of paired peaks: 86 WARNING @ Tue, 30 Jun 2020 00:39:43: Too few paired peaks (86) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:39:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX706809/SRX706809.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX706809/SRX706809.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX706809/SRX706809.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX706809/SRX706809.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:39:47: 15000000 INFO @ Tue, 30 Jun 2020 00:39:53: 16000000 INFO @ Tue, 30 Jun 2020 00:40:00: 17000000 INFO @ Tue, 30 Jun 2020 00:40:06: 18000000 INFO @ Tue, 30 Jun 2020 00:40:13: 19000000 INFO @ Tue, 30 Jun 2020 00:40:15: #1 tag size is determined as 44 bps INFO @ Tue, 30 Jun 2020 00:40:15: #1 tag size = 44 INFO @ Tue, 30 Jun 2020 00:40:15: #1 total tags in treatment: 19318296 INFO @ Tue, 30 Jun 2020 00:40:15: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:40:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:40:15: #1 tags after filtering in treatment: 19318296 INFO @ Tue, 30 Jun 2020 00:40:15: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:40:15: #1 finished! INFO @ Tue, 30 Jun 2020 00:40:15: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:40:15: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:40:16: #2 number of paired peaks: 86 WARNING @ Tue, 30 Jun 2020 00:40:16: Too few paired peaks (86) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:40:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX706809/SRX706809.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX706809/SRX706809.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX706809/SRX706809.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX706809/SRX706809.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。