Job ID = 6528471 SRX = SRX6962951 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T15:11:18 prefetch.2.10.7: 1) Downloading 'SRR10244311'... 2020-06-29T15:11:18 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:14:22 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:14:22 prefetch.2.10.7: 'SRR10244311' is valid 2020-06-29T15:14:22 prefetch.2.10.7: 1) 'SRR10244311' was downloaded successfully 2020-06-29T15:14:22 prefetch.2.10.7: 'SRR10244311' has 0 unresolved dependencies Read 12039586 spots for SRR10244311/SRR10244311.sra Written 12039586 spots for SRR10244311/SRR10244311.sra 2020-06-29T15:15:17 prefetch.2.10.7: 1) Downloading 'SRR10244312'... 2020-06-29T15:15:17 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:17:27 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:17:28 prefetch.2.10.7: 'SRR10244312' is valid 2020-06-29T15:17:28 prefetch.2.10.7: 1) 'SRR10244312' was downloaded successfully 2020-06-29T15:17:28 prefetch.2.10.7: 'SRR10244312' has 0 unresolved dependencies Read 11952612 spots for SRR10244312/SRR10244312.sra Written 11952612 spots for SRR10244312/SRR10244312.sra 2020-06-29T15:18:22 prefetch.2.10.7: 1) Downloading 'SRR10244313'... 2020-06-29T15:18:22 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:19:34 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:19:35 prefetch.2.10.7: 'SRR10244313' is valid 2020-06-29T15:19:35 prefetch.2.10.7: 1) 'SRR10244313' was downloaded successfully 2020-06-29T15:19:35 prefetch.2.10.7: 'SRR10244313' has 0 unresolved dependencies Read 12625695 spots for SRR10244313/SRR10244313.sra Written 12625695 spots for SRR10244313/SRR10244313.sra 2020-06-29T15:20:32 prefetch.2.10.7: 1) Downloading 'SRR10244314'... 2020-06-29T15:20:32 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:21:48 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:21:49 prefetch.2.10.7: 'SRR10244314' is valid 2020-06-29T15:21:49 prefetch.2.10.7: 1) 'SRR10244314' was downloaded successfully 2020-06-29T15:21:49 prefetch.2.10.7: 'SRR10244314' has 0 unresolved dependencies Read 12489155 spots for SRR10244314/SRR10244314.sra Written 12489155 spots for SRR10244314/SRR10244314.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:19:15 49107048 reads; of these: 49107048 (100.00%) were unpaired; of these: 4756205 (9.69%) aligned 0 times 34341324 (69.93%) aligned exactly 1 time 10009519 (20.38%) aligned >1 times 90.31% overall alignment rate Time searching: 00:19:15 Overall time: 00:19:15 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 13435948 / 44350843 = 0.3029 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:16:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6962951/SRX6962951.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6962951/SRX6962951.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6962951/SRX6962951.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6962951/SRX6962951.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:16:33: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:16:33: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:16:40: 1000000 INFO @ Tue, 30 Jun 2020 01:16:47: 2000000 INFO @ Tue, 30 Jun 2020 01:16:54: 3000000 INFO @ Tue, 30 Jun 2020 01:17:01: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:17:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6962951/SRX6962951.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6962951/SRX6962951.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6962951/SRX6962951.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6962951/SRX6962951.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:17:03: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:17:03: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:17:08: 5000000 INFO @ Tue, 30 Jun 2020 01:17:11: 1000000 INFO @ Tue, 30 Jun 2020 01:17:16: 6000000 INFO @ Tue, 30 Jun 2020 01:17:19: 2000000 INFO @ Tue, 30 Jun 2020 01:17:25: 7000000 INFO @ Tue, 30 Jun 2020 01:17:27: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:17:33: 8000000 INFO @ Tue, 30 Jun 2020 01:17:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6962951/SRX6962951.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6962951/SRX6962951.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6962951/SRX6962951.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6962951/SRX6962951.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:17:33: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:17:33: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:17:35: 4000000 INFO @ Tue, 30 Jun 2020 01:17:41: 9000000 INFO @ Tue, 30 Jun 2020 01:17:41: 1000000 INFO @ Tue, 30 Jun 2020 01:17:43: 5000000 INFO @ Tue, 30 Jun 2020 01:17:49: 2000000 INFO @ Tue, 30 Jun 2020 01:17:49: 10000000 INFO @ Tue, 30 Jun 2020 01:17:51: 6000000 INFO @ Tue, 30 Jun 2020 01:17:58: 3000000 INFO @ Tue, 30 Jun 2020 01:17:58: 11000000 INFO @ Tue, 30 Jun 2020 01:17:59: 7000000 INFO @ Tue, 30 Jun 2020 01:18:06: 4000000 INFO @ Tue, 30 Jun 2020 01:18:06: 12000000 INFO @ Tue, 30 Jun 2020 01:18:08: 8000000 INFO @ Tue, 30 Jun 2020 01:18:14: 5000000 INFO @ Tue, 30 Jun 2020 01:18:15: 13000000 INFO @ Tue, 30 Jun 2020 01:18:16: 9000000 INFO @ Tue, 30 Jun 2020 01:18:22: 6000000 INFO @ Tue, 30 Jun 2020 01:18:23: 14000000 INFO @ Tue, 30 Jun 2020 01:18:24: 10000000 INFO @ Tue, 30 Jun 2020 01:18:30: 7000000 INFO @ Tue, 30 Jun 2020 01:18:32: 15000000 INFO @ Tue, 30 Jun 2020 01:18:32: 11000000 INFO @ Tue, 30 Jun 2020 01:18:39: 8000000 INFO @ Tue, 30 Jun 2020 01:18:40: 16000000 INFO @ Tue, 30 Jun 2020 01:18:41: 12000000 INFO @ Tue, 30 Jun 2020 01:18:47: 9000000 INFO @ Tue, 30 Jun 2020 01:18:49: 17000000 INFO @ Tue, 30 Jun 2020 01:18:49: 13000000 INFO @ Tue, 30 Jun 2020 01:18:56: 10000000 INFO @ Tue, 30 Jun 2020 01:18:57: 18000000 INFO @ Tue, 30 Jun 2020 01:18:58: 14000000 INFO @ Tue, 30 Jun 2020 01:19:04: 11000000 INFO @ Tue, 30 Jun 2020 01:19:05: 19000000 INFO @ Tue, 30 Jun 2020 01:19:06: 15000000 INFO @ Tue, 30 Jun 2020 01:19:13: 12000000 INFO @ Tue, 30 Jun 2020 01:19:14: 20000000 INFO @ Tue, 30 Jun 2020 01:19:15: 16000000 INFO @ Tue, 30 Jun 2020 01:19:21: 13000000 INFO @ Tue, 30 Jun 2020 01:19:22: 21000000 INFO @ Tue, 30 Jun 2020 01:19:23: 17000000 INFO @ Tue, 30 Jun 2020 01:19:29: 14000000 INFO @ Tue, 30 Jun 2020 01:19:30: 22000000 INFO @ Tue, 30 Jun 2020 01:19:31: 18000000 INFO @ Tue, 30 Jun 2020 01:19:38: 15000000 INFO @ Tue, 30 Jun 2020 01:19:39: 23000000 INFO @ Tue, 30 Jun 2020 01:19:40: 19000000 INFO @ Tue, 30 Jun 2020 01:19:47: 16000000 INFO @ Tue, 30 Jun 2020 01:19:47: 24000000 INFO @ Tue, 30 Jun 2020 01:19:49: 20000000 INFO @ Tue, 30 Jun 2020 01:19:56: 17000000 INFO @ Tue, 30 Jun 2020 01:19:56: 25000000 INFO @ Tue, 30 Jun 2020 01:19:57: 21000000 INFO @ Tue, 30 Jun 2020 01:20:05: 18000000 INFO @ Tue, 30 Jun 2020 01:20:05: 26000000 INFO @ Tue, 30 Jun 2020 01:20:06: 22000000 INFO @ Tue, 30 Jun 2020 01:20:13: 19000000 INFO @ Tue, 30 Jun 2020 01:20:13: 27000000 INFO @ Tue, 30 Jun 2020 01:20:15: 23000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 01:20:22: 28000000 INFO @ Tue, 30 Jun 2020 01:20:22: 20000000 INFO @ Tue, 30 Jun 2020 01:20:24: 24000000 INFO @ Tue, 30 Jun 2020 01:20:30: 29000000 INFO @ Tue, 30 Jun 2020 01:20:31: 21000000 INFO @ Tue, 30 Jun 2020 01:20:32: 25000000 INFO @ Tue, 30 Jun 2020 01:20:39: 30000000 INFO @ Tue, 30 Jun 2020 01:20:39: 22000000 INFO @ Tue, 30 Jun 2020 01:20:41: 26000000 INFO @ Tue, 30 Jun 2020 01:20:47: #1 tag size is determined as 75 bps INFO @ Tue, 30 Jun 2020 01:20:47: #1 tag size = 75 INFO @ Tue, 30 Jun 2020 01:20:47: #1 total tags in treatment: 30914895 INFO @ Tue, 30 Jun 2020 01:20:47: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:20:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:20:48: #1 tags after filtering in treatment: 30914895 INFO @ Tue, 30 Jun 2020 01:20:48: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:20:48: #1 finished! INFO @ Tue, 30 Jun 2020 01:20:48: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:20:48: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:20:48: 23000000 INFO @ Tue, 30 Jun 2020 01:20:50: #2 number of paired peaks: 2 WARNING @ Tue, 30 Jun 2020 01:20:50: Too few paired peaks (2) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:20:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6962951/SRX6962951.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6962951/SRX6962951.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6962951/SRX6962951.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6962951/SRX6962951.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 01:20:50: 27000000 INFO @ Tue, 30 Jun 2020 01:20:57: 24000000 INFO @ Tue, 30 Jun 2020 01:20:58: 28000000 INFO @ Tue, 30 Jun 2020 01:21:05: 25000000 INFO @ Tue, 30 Jun 2020 01:21:07: 29000000 INFO @ Tue, 30 Jun 2020 01:21:14: 26000000 INFO @ Tue, 30 Jun 2020 01:21:15: 30000000 INFO @ Tue, 30 Jun 2020 01:21:22: 27000000 INFO @ Tue, 30 Jun 2020 01:21:23: #1 tag size is determined as 75 bps INFO @ Tue, 30 Jun 2020 01:21:23: #1 tag size = 75 INFO @ Tue, 30 Jun 2020 01:21:23: #1 total tags in treatment: 30914895 INFO @ Tue, 30 Jun 2020 01:21:23: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:21:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:21:24: #1 tags after filtering in treatment: 30914895 INFO @ Tue, 30 Jun 2020 01:21:24: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:21:24: #1 finished! INFO @ Tue, 30 Jun 2020 01:21:24: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:21:24: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:21:26: #2 number of paired peaks: 2 WARNING @ Tue, 30 Jun 2020 01:21:26: Too few paired peaks (2) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:21:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6962951/SRX6962951.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6962951/SRX6962951.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6962951/SRX6962951.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6962951/SRX6962951.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Tue, 30 Jun 2020 01:21:30: 28000000 INFO @ Tue, 30 Jun 2020 01:21:37: 29000000 INFO @ Tue, 30 Jun 2020 01:21:45: 30000000 INFO @ Tue, 30 Jun 2020 01:21:52: #1 tag size is determined as 75 bps INFO @ Tue, 30 Jun 2020 01:21:52: #1 tag size = 75 INFO @ Tue, 30 Jun 2020 01:21:52: #1 total tags in treatment: 30914895 INFO @ Tue, 30 Jun 2020 01:21:52: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:21:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:21:52: #1 tags after filtering in treatment: 30914895 INFO @ Tue, 30 Jun 2020 01:21:52: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:21:52: #1 finished! INFO @ Tue, 30 Jun 2020 01:21:52: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:21:52: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:21:54: #2 number of paired peaks: 2 WARNING @ Tue, 30 Jun 2020 01:21:54: Too few paired peaks (2) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:21:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6962951/SRX6962951.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6962951/SRX6962951.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6962951/SRX6962951.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6962951/SRX6962951.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling