Job ID = 6528468 SRX = SRX6962948 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T15:20:04 prefetch.2.10.7: 1) Downloading 'SRR10244299'... 2020-06-29T15:20:04 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:21:06 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:21:07 prefetch.2.10.7: 'SRR10244299' is valid 2020-06-29T15:21:07 prefetch.2.10.7: 1) 'SRR10244299' was downloaded successfully 2020-06-29T15:21:07 prefetch.2.10.7: 'SRR10244299' has 0 unresolved dependencies Read 12014540 spots for SRR10244299/SRR10244299.sra Written 12014540 spots for SRR10244299/SRR10244299.sra 2020-06-29T15:21:58 prefetch.2.10.7: 1) Downloading 'SRR10244300'... 2020-06-29T15:21:58 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:23:04 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:23:05 prefetch.2.10.7: 'SRR10244300' is valid 2020-06-29T15:23:05 prefetch.2.10.7: 1) 'SRR10244300' was downloaded successfully 2020-06-29T15:23:05 prefetch.2.10.7: 'SRR10244300' has 0 unresolved dependencies Read 11897046 spots for SRR10244300/SRR10244300.sra Written 11897046 spots for SRR10244300/SRR10244300.sra 2020-06-29T15:23:56 prefetch.2.10.7: 1) Downloading 'SRR10244301'... 2020-06-29T15:23:56 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:26:05 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:26:06 prefetch.2.10.7: 'SRR10244301' is valid 2020-06-29T15:26:06 prefetch.2.10.7: 1) 'SRR10244301' was downloaded successfully 2020-06-29T15:26:06 prefetch.2.10.7: 'SRR10244301' has 0 unresolved dependencies Read 12544893 spots for SRR10244301/SRR10244301.sra Written 12544893 spots for SRR10244301/SRR10244301.sra 2020-06-29T15:27:02 prefetch.2.10.7: 1) Downloading 'SRR10244302'... 2020-06-29T15:27:02 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:28:41 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:28:42 prefetch.2.10.7: 'SRR10244302' is valid 2020-06-29T15:28:42 prefetch.2.10.7: 1) 'SRR10244302' was downloaded successfully 2020-06-29T15:28:42 prefetch.2.10.7: 'SRR10244302' has 0 unresolved dependencies Read 12400921 spots for SRR10244302/SRR10244302.sra Written 12400921 spots for SRR10244302/SRR10244302.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:16:28 48857400 reads; of these: 48857400 (100.00%) were unpaired; of these: 17434066 (35.68%) aligned 0 times 22395781 (45.84%) aligned exactly 1 time 9027553 (18.48%) aligned >1 times 64.32% overall alignment rate Time searching: 00:16:28 Overall time: 00:16:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 6866807 / 31423334 = 0.2185 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:14:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6962948/SRX6962948.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6962948/SRX6962948.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6962948/SRX6962948.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6962948/SRX6962948.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:14:22: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:14:22: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:14:27: 1000000 INFO @ Tue, 30 Jun 2020 01:14:32: 2000000 INFO @ Tue, 30 Jun 2020 01:14:38: 3000000 INFO @ Tue, 30 Jun 2020 01:14:43: 4000000 INFO @ Tue, 30 Jun 2020 01:14:48: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:14:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6962948/SRX6962948.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6962948/SRX6962948.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6962948/SRX6962948.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6962948/SRX6962948.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:14:52: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:14:52: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:14:54: 6000000 INFO @ Tue, 30 Jun 2020 01:14:58: 1000000 INFO @ Tue, 30 Jun 2020 01:14:59: 7000000 INFO @ Tue, 30 Jun 2020 01:15:05: 2000000 INFO @ Tue, 30 Jun 2020 01:15:05: 8000000 INFO @ Tue, 30 Jun 2020 01:15:11: 9000000 INFO @ Tue, 30 Jun 2020 01:15:12: 3000000 INFO @ Tue, 30 Jun 2020 01:15:17: 10000000 INFO @ Tue, 30 Jun 2020 01:15:18: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:15:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6962948/SRX6962948.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6962948/SRX6962948.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6962948/SRX6962948.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6962948/SRX6962948.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:15:22: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:15:22: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:15:22: 11000000 INFO @ Tue, 30 Jun 2020 01:15:25: 5000000 INFO @ Tue, 30 Jun 2020 01:15:28: 12000000 INFO @ Tue, 30 Jun 2020 01:15:28: 1000000 INFO @ Tue, 30 Jun 2020 01:15:32: 6000000 INFO @ Tue, 30 Jun 2020 01:15:33: 13000000 INFO @ Tue, 30 Jun 2020 01:15:35: 2000000 INFO @ Tue, 30 Jun 2020 01:15:38: 7000000 INFO @ Tue, 30 Jun 2020 01:15:39: 14000000 INFO @ Tue, 30 Jun 2020 01:15:42: 3000000 INFO @ Tue, 30 Jun 2020 01:15:45: 15000000 INFO @ Tue, 30 Jun 2020 01:15:45: 8000000 INFO @ Tue, 30 Jun 2020 01:15:48: 4000000 INFO @ Tue, 30 Jun 2020 01:15:50: 16000000 INFO @ Tue, 30 Jun 2020 01:15:52: 9000000 INFO @ Tue, 30 Jun 2020 01:15:55: 5000000 INFO @ Tue, 30 Jun 2020 01:15:56: 17000000 INFO @ Tue, 30 Jun 2020 01:15:58: 10000000 INFO @ Tue, 30 Jun 2020 01:16:01: 18000000 INFO @ Tue, 30 Jun 2020 01:16:02: 6000000 INFO @ Tue, 30 Jun 2020 01:16:05: 11000000 INFO @ Tue, 30 Jun 2020 01:16:07: 19000000 INFO @ Tue, 30 Jun 2020 01:16:08: 7000000 INFO @ Tue, 30 Jun 2020 01:16:11: 12000000 INFO @ Tue, 30 Jun 2020 01:16:12: 20000000 INFO @ Tue, 30 Jun 2020 01:16:15: 8000000 INFO @ Tue, 30 Jun 2020 01:16:18: 13000000 INFO @ Tue, 30 Jun 2020 01:16:18: 21000000 INFO @ Tue, 30 Jun 2020 01:16:22: 9000000 INFO @ Tue, 30 Jun 2020 01:16:24: 22000000 INFO @ Tue, 30 Jun 2020 01:16:24: 14000000 INFO @ Tue, 30 Jun 2020 01:16:28: 10000000 INFO @ Tue, 30 Jun 2020 01:16:29: 23000000 INFO @ Tue, 30 Jun 2020 01:16:31: 15000000 INFO @ Tue, 30 Jun 2020 01:16:35: 11000000 INFO @ Tue, 30 Jun 2020 01:16:35: 24000000 INFO @ Tue, 30 Jun 2020 01:16:37: 16000000 INFO @ Tue, 30 Jun 2020 01:16:38: #1 tag size is determined as 75 bps INFO @ Tue, 30 Jun 2020 01:16:38: #1 tag size = 75 INFO @ Tue, 30 Jun 2020 01:16:38: #1 total tags in treatment: 24556527 INFO @ Tue, 30 Jun 2020 01:16:38: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:16:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:16:38: #1 tags after filtering in treatment: 24556527 INFO @ Tue, 30 Jun 2020 01:16:38: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:16:38: #1 finished! INFO @ Tue, 30 Jun 2020 01:16:38: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:16:38: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:16:40: #2 number of paired peaks: 9 WARNING @ Tue, 30 Jun 2020 01:16:40: Too few paired peaks (9) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:16:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6962948/SRX6962948.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6962948/SRX6962948.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6962948/SRX6962948.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6962948/SRX6962948.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 01:16:41: 12000000 INFO @ Tue, 30 Jun 2020 01:16:43: 17000000 INFO @ Tue, 30 Jun 2020 01:16:48: 13000000 INFO @ Tue, 30 Jun 2020 01:16:50: 18000000 INFO @ Tue, 30 Jun 2020 01:16:54: 14000000 INFO @ Tue, 30 Jun 2020 01:16:56: 19000000 INFO @ Tue, 30 Jun 2020 01:17:01: 15000000 INFO @ Tue, 30 Jun 2020 01:17:03: 20000000 INFO @ Tue, 30 Jun 2020 01:17:07: 16000000 INFO @ Tue, 30 Jun 2020 01:17:09: 21000000 INFO @ Tue, 30 Jun 2020 01:17:13: 17000000 INFO @ Tue, 30 Jun 2020 01:17:15: 22000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 01:17:20: 18000000 INFO @ Tue, 30 Jun 2020 01:17:22: 23000000 INFO @ Tue, 30 Jun 2020 01:17:26: 19000000 INFO @ Tue, 30 Jun 2020 01:17:28: 24000000 INFO @ Tue, 30 Jun 2020 01:17:32: #1 tag size is determined as 75 bps INFO @ Tue, 30 Jun 2020 01:17:32: #1 tag size = 75 INFO @ Tue, 30 Jun 2020 01:17:32: #1 total tags in treatment: 24556527 INFO @ Tue, 30 Jun 2020 01:17:32: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:17:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:17:32: #1 tags after filtering in treatment: 24556527 INFO @ Tue, 30 Jun 2020 01:17:32: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:17:32: #1 finished! INFO @ Tue, 30 Jun 2020 01:17:32: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:17:32: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:17:32: 20000000 INFO @ Tue, 30 Jun 2020 01:17:33: #2 number of paired peaks: 9 WARNING @ Tue, 30 Jun 2020 01:17:33: Too few paired peaks (9) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:17:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6962948/SRX6962948.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6962948/SRX6962948.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6962948/SRX6962948.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6962948/SRX6962948.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 01:17:39: 21000000 INFO @ Tue, 30 Jun 2020 01:17:45: 22000000 INFO @ Tue, 30 Jun 2020 01:17:51: 23000000 INFO @ Tue, 30 Jun 2020 01:17:57: 24000000 INFO @ Tue, 30 Jun 2020 01:18:01: #1 tag size is determined as 75 bps INFO @ Tue, 30 Jun 2020 01:18:01: #1 tag size = 75 INFO @ Tue, 30 Jun 2020 01:18:01: #1 total tags in treatment: 24556527 INFO @ Tue, 30 Jun 2020 01:18:01: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:18:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:18:01: #1 tags after filtering in treatment: 24556527 INFO @ Tue, 30 Jun 2020 01:18:01: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:18:01: #1 finished! INFO @ Tue, 30 Jun 2020 01:18:01: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:18:01: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:18:03: #2 number of paired peaks: 9 WARNING @ Tue, 30 Jun 2020 01:18:03: Too few paired peaks (9) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:18:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6962948/SRX6962948.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6962948/SRX6962948.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6962948/SRX6962948.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6962948/SRX6962948.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。