Job ID = 6528465 SRX = SRX6827254 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T15:25:35 prefetch.2.10.7: 1) Downloading 'SRR10094667'... 2020-06-29T15:25:35 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:30:45 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:30:45 prefetch.2.10.7: 1) 'SRR10094667' was downloaded successfully Read 36391993 spots for SRR10094667/SRR10094667.sra Written 36391993 spots for SRR10094667/SRR10094667.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:17 36391993 reads; of these: 36391993 (100.00%) were unpaired; of these: 14679742 (40.34%) aligned 0 times 18715478 (51.43%) aligned exactly 1 time 2996773 (8.23%) aligned >1 times 59.66% overall alignment rate Time searching: 00:07:17 Overall time: 00:07:17 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 3170720 / 21712251 = 0.1460 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:52:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6827254/SRX6827254.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6827254/SRX6827254.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6827254/SRX6827254.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6827254/SRX6827254.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:52:21: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:52:21: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:52:27: 1000000 INFO @ Tue, 30 Jun 2020 00:52:32: 2000000 INFO @ Tue, 30 Jun 2020 00:52:38: 3000000 INFO @ Tue, 30 Jun 2020 00:52:44: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:52:50: 5000000 INFO @ Tue, 30 Jun 2020 00:52:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6827254/SRX6827254.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6827254/SRX6827254.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6827254/SRX6827254.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6827254/SRX6827254.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:52:51: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:52:51: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:52:56: 6000000 INFO @ Tue, 30 Jun 2020 00:52:57: 1000000 INFO @ Tue, 30 Jun 2020 00:53:02: 7000000 INFO @ Tue, 30 Jun 2020 00:53:03: 2000000 INFO @ Tue, 30 Jun 2020 00:53:08: 8000000 INFO @ Tue, 30 Jun 2020 00:53:09: 3000000 INFO @ Tue, 30 Jun 2020 00:53:14: 9000000 INFO @ Tue, 30 Jun 2020 00:53:15: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:53:20: 10000000 INFO @ Tue, 30 Jun 2020 00:53:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6827254/SRX6827254.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6827254/SRX6827254.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6827254/SRX6827254.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6827254/SRX6827254.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:53:21: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:53:21: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:53:21: 5000000 INFO @ Tue, 30 Jun 2020 00:53:26: 11000000 INFO @ Tue, 30 Jun 2020 00:53:26: 1000000 INFO @ Tue, 30 Jun 2020 00:53:27: 6000000 INFO @ Tue, 30 Jun 2020 00:53:32: 2000000 INFO @ Tue, 30 Jun 2020 00:53:32: 12000000 INFO @ Tue, 30 Jun 2020 00:53:34: 7000000 INFO @ Tue, 30 Jun 2020 00:53:37: 3000000 INFO @ Tue, 30 Jun 2020 00:53:38: 13000000 INFO @ Tue, 30 Jun 2020 00:53:39: 8000000 INFO @ Tue, 30 Jun 2020 00:53:42: 4000000 INFO @ Tue, 30 Jun 2020 00:53:44: 14000000 INFO @ Tue, 30 Jun 2020 00:53:45: 9000000 INFO @ Tue, 30 Jun 2020 00:53:48: 5000000 INFO @ Tue, 30 Jun 2020 00:53:49: 15000000 INFO @ Tue, 30 Jun 2020 00:53:51: 10000000 INFO @ Tue, 30 Jun 2020 00:53:53: 6000000 INFO @ Tue, 30 Jun 2020 00:53:55: 16000000 INFO @ Tue, 30 Jun 2020 00:53:58: 11000000 INFO @ Tue, 30 Jun 2020 00:53:59: 7000000 INFO @ Tue, 30 Jun 2020 00:54:01: 17000000 INFO @ Tue, 30 Jun 2020 00:54:04: 12000000 INFO @ Tue, 30 Jun 2020 00:54:04: 8000000 INFO @ Tue, 30 Jun 2020 00:54:07: 18000000 INFO @ Tue, 30 Jun 2020 00:54:10: 13000000 INFO @ Tue, 30 Jun 2020 00:54:10: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:54:10: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:54:10: #1 total tags in treatment: 18541531 INFO @ Tue, 30 Jun 2020 00:54:10: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:54:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:54:10: 9000000 INFO @ Tue, 30 Jun 2020 00:54:10: #1 tags after filtering in treatment: 18541531 INFO @ Tue, 30 Jun 2020 00:54:10: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:54:10: #1 finished! INFO @ Tue, 30 Jun 2020 00:54:10: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:54:10: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:54:12: #2 number of paired peaks: 23 WARNING @ Tue, 30 Jun 2020 00:54:12: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:54:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6827254/SRX6827254.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6827254/SRX6827254.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6827254/SRX6827254.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6827254/SRX6827254.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:54:16: 14000000 INFO @ Tue, 30 Jun 2020 00:54:16: 10000000 INFO @ Tue, 30 Jun 2020 00:54:21: 11000000 INFO @ Tue, 30 Jun 2020 00:54:21: 15000000 INFO @ Tue, 30 Jun 2020 00:54:26: 12000000 INFO @ Tue, 30 Jun 2020 00:54:27: 16000000 INFO @ Tue, 30 Jun 2020 00:54:32: 13000000 INFO @ Tue, 30 Jun 2020 00:54:33: 17000000 INFO @ Tue, 30 Jun 2020 00:54:37: 14000000 INFO @ Tue, 30 Jun 2020 00:54:39: 18000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:54:42: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:54:42: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:54:42: #1 total tags in treatment: 18541531 INFO @ Tue, 30 Jun 2020 00:54:42: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:54:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:54:42: #1 tags after filtering in treatment: 18541531 INFO @ Tue, 30 Jun 2020 00:54:42: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:54:42: #1 finished! INFO @ Tue, 30 Jun 2020 00:54:42: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:54:42: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:54:43: 15000000 INFO @ Tue, 30 Jun 2020 00:54:43: #2 number of paired peaks: 23 WARNING @ Tue, 30 Jun 2020 00:54:43: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:54:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6827254/SRX6827254.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6827254/SRX6827254.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6827254/SRX6827254.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6827254/SRX6827254.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:54:48: 16000000 INFO @ Tue, 30 Jun 2020 00:54:53: 17000000 INFO @ Tue, 30 Jun 2020 00:54:58: 18000000 INFO @ Tue, 30 Jun 2020 00:55:01: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:55:01: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:55:01: #1 total tags in treatment: 18541531 INFO @ Tue, 30 Jun 2020 00:55:01: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:55:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:55:02: #1 tags after filtering in treatment: 18541531 INFO @ Tue, 30 Jun 2020 00:55:02: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:55:02: #1 finished! INFO @ Tue, 30 Jun 2020 00:55:02: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:55:02: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:55:03: #2 number of paired peaks: 23 WARNING @ Tue, 30 Jun 2020 00:55:03: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:55:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6827254/SRX6827254.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6827254/SRX6827254.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6827254/SRX6827254.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6827254/SRX6827254.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。