Job ID = 4303134 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 28,962,974 reads read : 28,962,974 reads written : 28,962,974 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR1552299.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:22 28962974 reads; of these: 28962974 (100.00%) were unpaired; of these: 1432420 (4.95%) aligned 0 times 22789426 (78.68%) aligned exactly 1 time 4741128 (16.37%) aligned >1 times 95.05% overall alignment rate Time searching: 00:07:22 Overall time: 00:07:22 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 4259059 / 27530554 = 0.1547 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Thu, 12 Dec 2019 01:04:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX681830/SRX681830.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX681830/SRX681830.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX681830/SRX681830.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX681830/SRX681830.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 12 Dec 2019 01:04:47: #1 read tag files... INFO @ Thu, 12 Dec 2019 01:04:47: #1 read treatment tags... INFO @ Thu, 12 Dec 2019 01:04:54: 1000000 INFO @ Thu, 12 Dec 2019 01:05:02: 2000000 INFO @ Thu, 12 Dec 2019 01:05:10: 3000000 INFO @ Thu, 12 Dec 2019 01:05:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX681830/SRX681830.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX681830/SRX681830.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX681830/SRX681830.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX681830/SRX681830.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 12 Dec 2019 01:05:16: #1 read tag files... INFO @ Thu, 12 Dec 2019 01:05:16: #1 read treatment tags... INFO @ Thu, 12 Dec 2019 01:05:18: 4000000 INFO @ Thu, 12 Dec 2019 01:05:25: 1000000 INFO @ Thu, 12 Dec 2019 01:05:27: 5000000 INFO @ Thu, 12 Dec 2019 01:05:32: 2000000 INFO @ Thu, 12 Dec 2019 01:05:35: 6000000 INFO @ Thu, 12 Dec 2019 01:05:39: 3000000 INFO @ Thu, 12 Dec 2019 01:05:42: 7000000 BedGraph に変換中... INFO @ Thu, 12 Dec 2019 01:05:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX681830/SRX681830.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX681830/SRX681830.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX681830/SRX681830.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX681830/SRX681830.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 12 Dec 2019 01:05:46: #1 read tag files... INFO @ Thu, 12 Dec 2019 01:05:46: #1 read treatment tags... INFO @ Thu, 12 Dec 2019 01:05:46: 4000000 INFO @ Thu, 12 Dec 2019 01:05:50: 8000000 INFO @ Thu, 12 Dec 2019 01:05:54: 1000000 INFO @ Thu, 12 Dec 2019 01:05:55: 5000000 INFO @ Thu, 12 Dec 2019 01:05:58: 9000000 INFO @ Thu, 12 Dec 2019 01:06:03: 2000000 INFO @ Thu, 12 Dec 2019 01:06:04: 6000000 INFO @ Thu, 12 Dec 2019 01:06:07: 10000000 INFO @ Thu, 12 Dec 2019 01:06:11: 3000000 INFO @ Thu, 12 Dec 2019 01:06:12: 7000000 INFO @ Thu, 12 Dec 2019 01:06:15: 11000000 INFO @ Thu, 12 Dec 2019 01:06:20: 4000000 INFO @ Thu, 12 Dec 2019 01:06:21: 8000000 INFO @ Thu, 12 Dec 2019 01:06:23: 12000000 INFO @ Thu, 12 Dec 2019 01:06:28: 5000000 INFO @ Thu, 12 Dec 2019 01:06:29: 9000000 INFO @ Thu, 12 Dec 2019 01:06:32: 13000000 INFO @ Thu, 12 Dec 2019 01:06:37: 6000000 INFO @ Thu, 12 Dec 2019 01:06:38: 10000000 INFO @ Thu, 12 Dec 2019 01:06:40: 14000000 INFO @ Thu, 12 Dec 2019 01:06:45: 7000000 INFO @ Thu, 12 Dec 2019 01:06:46: 11000000 INFO @ Thu, 12 Dec 2019 01:06:48: 15000000 INFO @ Thu, 12 Dec 2019 01:06:54: 8000000 INFO @ Thu, 12 Dec 2019 01:06:55: 12000000 INFO @ Thu, 12 Dec 2019 01:06:57: 16000000 INFO @ Thu, 12 Dec 2019 01:07:02: 9000000 INFO @ Thu, 12 Dec 2019 01:07:03: 13000000 INFO @ Thu, 12 Dec 2019 01:07:06: 17000000 INFO @ Thu, 12 Dec 2019 01:07:11: 10000000 INFO @ Thu, 12 Dec 2019 01:07:12: 14000000 INFO @ Thu, 12 Dec 2019 01:07:14: 18000000 INFO @ Thu, 12 Dec 2019 01:07:19: 11000000 INFO @ Thu, 12 Dec 2019 01:07:22: 15000000 INFO @ Thu, 12 Dec 2019 01:07:23: 19000000 INFO @ Thu, 12 Dec 2019 01:07:28: 12000000 INFO @ Thu, 12 Dec 2019 01:07:30: 16000000 INFO @ Thu, 12 Dec 2019 01:07:31: 20000000 INFO @ Thu, 12 Dec 2019 01:07:36: 13000000 INFO @ Thu, 12 Dec 2019 01:07:39: 17000000 INFO @ Thu, 12 Dec 2019 01:07:40: 21000000 INFO @ Thu, 12 Dec 2019 01:07:45: 14000000 INFO @ Thu, 12 Dec 2019 01:07:48: 18000000 INFO @ Thu, 12 Dec 2019 01:07:48: 22000000 INFO @ Thu, 12 Dec 2019 01:07:54: 15000000 INFO @ Thu, 12 Dec 2019 01:07:56: 19000000 INFO @ Thu, 12 Dec 2019 01:07:57: 23000000 INFO @ Thu, 12 Dec 2019 01:07:59: #1 tag size is determined as 50 bps INFO @ Thu, 12 Dec 2019 01:07:59: #1 tag size = 50 INFO @ Thu, 12 Dec 2019 01:07:59: #1 total tags in treatment: 23271495 INFO @ Thu, 12 Dec 2019 01:07:59: #1 user defined the maximum tags... INFO @ Thu, 12 Dec 2019 01:07:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 12 Dec 2019 01:08:00: #1 tags after filtering in treatment: 23271495 INFO @ Thu, 12 Dec 2019 01:08:00: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 12 Dec 2019 01:08:00: #1 finished! INFO @ Thu, 12 Dec 2019 01:08:00: #2 Build Peak Model... INFO @ Thu, 12 Dec 2019 01:08:00: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 12 Dec 2019 01:08:01: #2 number of paired peaks: 58 WARNING @ Thu, 12 Dec 2019 01:08:01: Too few paired peaks (58) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 12 Dec 2019 01:08:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX681830/SRX681830.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX681830/SRX681830.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX681830/SRX681830.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX681830/SRX681830.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 12 Dec 2019 01:08:02: 16000000 INFO @ Thu, 12 Dec 2019 01:08:05: 20000000 INFO @ Thu, 12 Dec 2019 01:08:11: 17000000 INFO @ Thu, 12 Dec 2019 01:08:14: 21000000 INFO @ Thu, 12 Dec 2019 01:08:19: 18000000 INFO @ Thu, 12 Dec 2019 01:08:22: 22000000 INFO @ Thu, 12 Dec 2019 01:08:28: 19000000 INFO @ Thu, 12 Dec 2019 01:08:30: 23000000 INFO @ Thu, 12 Dec 2019 01:08:32: #1 tag size is determined as 50 bps INFO @ Thu, 12 Dec 2019 01:08:32: #1 tag size = 50 INFO @ Thu, 12 Dec 2019 01:08:32: #1 total tags in treatment: 23271495 INFO @ Thu, 12 Dec 2019 01:08:32: #1 user defined the maximum tags... INFO @ Thu, 12 Dec 2019 01:08:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 12 Dec 2019 01:08:33: #1 tags after filtering in treatment: 23271495 INFO @ Thu, 12 Dec 2019 01:08:33: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 12 Dec 2019 01:08:33: #1 finished! INFO @ Thu, 12 Dec 2019 01:08:33: #2 Build Peak Model... INFO @ Thu, 12 Dec 2019 01:08:33: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 12 Dec 2019 01:08:34: #2 number of paired peaks: 58 WARNING @ Thu, 12 Dec 2019 01:08:34: Too few paired peaks (58) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 12 Dec 2019 01:08:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX681830/SRX681830.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX681830/SRX681830.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX681830/SRX681830.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX681830/SRX681830.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 12 Dec 2019 01:08:36: 20000000 INFO @ Thu, 12 Dec 2019 01:08:44: 21000000 INFO @ Thu, 12 Dec 2019 01:08:51: 22000000 INFO @ Thu, 12 Dec 2019 01:08:59: 23000000 INFO @ Thu, 12 Dec 2019 01:09:02: #1 tag size is determined as 50 bps INFO @ Thu, 12 Dec 2019 01:09:02: #1 tag size = 50 INFO @ Thu, 12 Dec 2019 01:09:02: #1 total tags in treatment: 23271495 INFO @ Thu, 12 Dec 2019 01:09:02: #1 user defined the maximum tags... INFO @ Thu, 12 Dec 2019 01:09:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 12 Dec 2019 01:09:02: #1 tags after filtering in treatment: 23271495 INFO @ Thu, 12 Dec 2019 01:09:02: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 12 Dec 2019 01:09:02: #1 finished! INFO @ Thu, 12 Dec 2019 01:09:02: #2 Build Peak Model... INFO @ Thu, 12 Dec 2019 01:09:02: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 12 Dec 2019 01:09:03: #2 number of paired peaks: 58 WARNING @ Thu, 12 Dec 2019 01:09:03: Too few paired peaks (58) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 12 Dec 2019 01:09:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX681830/SRX681830.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX681830/SRX681830.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX681830/SRX681830.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX681830/SRX681830.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。