Job ID = 12265394 SRX = SRX6817547 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 67292037 spots for SRR10084538/SRR10084538.sra Written 67292037 spots for SRR10084538/SRR10084538.sra fastq に変換しました。 bowtie でマッピング中... Your job 12267102 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 02:25:55 67292037 reads; of these: 67292037 (100.00%) were paired; of these: 36751366 (54.61%) aligned concordantly 0 times 23106583 (34.34%) aligned concordantly exactly 1 time 7434088 (11.05%) aligned concordantly >1 times ---- 36751366 pairs aligned concordantly 0 times; of these: 4387971 (11.94%) aligned discordantly 1 time ---- 32363395 pairs aligned 0 times concordantly or discordantly; of these: 64726790 mates make up the pairs; of these: 59225812 (91.50%) aligned 0 times 1101467 (1.70%) aligned exactly 1 time 4399511 (6.80%) aligned >1 times 55.99% overall alignment rate Time searching: 02:25:55 Overall time: 02:25:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 56 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 10658369 / 34580602 = 0.3082 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 10:06:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6817547/SRX6817547.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6817547/SRX6817547.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6817547/SRX6817547.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6817547/SRX6817547.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 10:06:56: #1 read tag files... INFO @ Sat, 03 Apr 2021 10:06:56: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 10:07:04: 1000000 INFO @ Sat, 03 Apr 2021 10:07:13: 2000000 INFO @ Sat, 03 Apr 2021 10:07:22: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 10:07:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6817547/SRX6817547.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6817547/SRX6817547.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6817547/SRX6817547.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6817547/SRX6817547.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 10:07:26: #1 read tag files... INFO @ Sat, 03 Apr 2021 10:07:26: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 10:07:31: 4000000 INFO @ Sat, 03 Apr 2021 10:07:36: 1000000 INFO @ Sat, 03 Apr 2021 10:07:41: 5000000 INFO @ Sat, 03 Apr 2021 10:07:46: 2000000 INFO @ Sat, 03 Apr 2021 10:07:51: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 10:07:55: 3000000 INFO @ Sat, 03 Apr 2021 10:07:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6817547/SRX6817547.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6817547/SRX6817547.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6817547/SRX6817547.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6817547/SRX6817547.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 10:07:56: #1 read tag files... INFO @ Sat, 03 Apr 2021 10:07:56: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 10:08:01: 7000000 INFO @ Sat, 03 Apr 2021 10:08:06: 4000000 INFO @ Sat, 03 Apr 2021 10:08:06: 1000000 INFO @ Sat, 03 Apr 2021 10:08:11: 8000000 INFO @ Sat, 03 Apr 2021 10:08:16: 5000000 INFO @ Sat, 03 Apr 2021 10:08:16: 2000000 INFO @ Sat, 03 Apr 2021 10:08:21: 9000000 INFO @ Sat, 03 Apr 2021 10:08:26: 6000000 INFO @ Sat, 03 Apr 2021 10:08:27: 3000000 INFO @ Sat, 03 Apr 2021 10:08:31: 10000000 INFO @ Sat, 03 Apr 2021 10:08:37: 7000000 INFO @ Sat, 03 Apr 2021 10:08:37: 4000000 INFO @ Sat, 03 Apr 2021 10:08:41: 11000000 INFO @ Sat, 03 Apr 2021 10:08:47: 8000000 INFO @ Sat, 03 Apr 2021 10:08:47: 5000000 INFO @ Sat, 03 Apr 2021 10:08:50: 12000000 INFO @ Sat, 03 Apr 2021 10:08:57: 9000000 INFO @ Sat, 03 Apr 2021 10:08:58: 6000000 INFO @ Sat, 03 Apr 2021 10:09:00: 13000000 INFO @ Sat, 03 Apr 2021 10:09:07: 10000000 INFO @ Sat, 03 Apr 2021 10:09:08: 7000000 INFO @ Sat, 03 Apr 2021 10:09:10: 14000000 INFO @ Sat, 03 Apr 2021 10:09:17: 11000000 INFO @ Sat, 03 Apr 2021 10:09:18: 8000000 INFO @ Sat, 03 Apr 2021 10:09:20: 15000000 INFO @ Sat, 03 Apr 2021 10:09:27: 12000000 INFO @ Sat, 03 Apr 2021 10:09:28: 9000000 INFO @ Sat, 03 Apr 2021 10:09:29: 16000000 INFO @ Sat, 03 Apr 2021 10:09:37: 13000000 INFO @ Sat, 03 Apr 2021 10:09:38: 10000000 INFO @ Sat, 03 Apr 2021 10:09:39: 17000000 INFO @ Sat, 03 Apr 2021 10:09:47: 14000000 INFO @ Sat, 03 Apr 2021 10:09:48: 11000000 INFO @ Sat, 03 Apr 2021 10:09:49: 18000000 INFO @ Sat, 03 Apr 2021 10:09:57: 15000000 INFO @ Sat, 03 Apr 2021 10:09:58: 12000000 INFO @ Sat, 03 Apr 2021 10:09:58: 19000000 INFO @ Sat, 03 Apr 2021 10:10:07: 16000000 INFO @ Sat, 03 Apr 2021 10:10:08: 13000000 INFO @ Sat, 03 Apr 2021 10:10:08: 20000000 INFO @ Sat, 03 Apr 2021 10:10:17: 17000000 INFO @ Sat, 03 Apr 2021 10:10:17: 14000000 INFO @ Sat, 03 Apr 2021 10:10:18: 21000000 INFO @ Sat, 03 Apr 2021 10:10:26: 18000000 INFO @ Sat, 03 Apr 2021 10:10:27: 22000000 INFO @ Sat, 03 Apr 2021 10:10:27: 15000000 INFO @ Sat, 03 Apr 2021 10:10:36: 19000000 INFO @ Sat, 03 Apr 2021 10:10:37: 23000000 INFO @ Sat, 03 Apr 2021 10:10:37: 16000000 INFO @ Sat, 03 Apr 2021 10:10:46: 20000000 INFO @ Sat, 03 Apr 2021 10:10:47: 24000000 INFO @ Sat, 03 Apr 2021 10:10:47: 17000000 INFO @ Sat, 03 Apr 2021 10:10:56: 21000000 INFO @ Sat, 03 Apr 2021 10:10:56: 25000000 INFO @ Sat, 03 Apr 2021 10:10:57: 18000000 INFO @ Sat, 03 Apr 2021 10:11:06: 22000000 INFO @ Sat, 03 Apr 2021 10:11:06: 26000000 INFO @ Sat, 03 Apr 2021 10:11:06: 19000000 INFO @ Sat, 03 Apr 2021 10:11:15: 23000000 INFO @ Sat, 03 Apr 2021 10:11:16: 27000000 INFO @ Sat, 03 Apr 2021 10:11:16: 20000000 INFO @ Sat, 03 Apr 2021 10:11:25: 24000000 INFO @ Sat, 03 Apr 2021 10:11:26: 28000000 INFO @ Sat, 03 Apr 2021 10:11:26: 21000000 INFO @ Sat, 03 Apr 2021 10:11:35: 25000000 INFO @ Sat, 03 Apr 2021 10:11:36: 29000000 INFO @ Sat, 03 Apr 2021 10:11:36: 22000000 INFO @ Sat, 03 Apr 2021 10:11:45: 26000000 INFO @ Sat, 03 Apr 2021 10:11:46: 30000000 INFO @ Sat, 03 Apr 2021 10:11:46: 23000000 INFO @ Sat, 03 Apr 2021 10:11:55: 31000000 INFO @ Sat, 03 Apr 2021 10:11:55: 27000000 INFO @ Sat, 03 Apr 2021 10:11:56: 24000000 INFO @ Sat, 03 Apr 2021 10:12:05: 32000000 INFO @ Sat, 03 Apr 2021 10:12:05: 28000000 INFO @ Sat, 03 Apr 2021 10:12:06: 25000000 INFO @ Sat, 03 Apr 2021 10:12:14: 33000000 INFO @ Sat, 03 Apr 2021 10:12:15: 29000000 INFO @ Sat, 03 Apr 2021 10:12:16: 26000000 INFO @ Sat, 03 Apr 2021 10:12:24: 34000000 INFO @ Sat, 03 Apr 2021 10:12:25: 30000000 INFO @ Sat, 03 Apr 2021 10:12:26: 27000000 INFO @ Sat, 03 Apr 2021 10:12:33: 35000000 INFO @ Sat, 03 Apr 2021 10:12:35: 31000000 INFO @ Sat, 03 Apr 2021 10:12:36: 28000000 INFO @ Sat, 03 Apr 2021 10:12:43: 36000000 INFO @ Sat, 03 Apr 2021 10:12:45: 32000000 INFO @ Sat, 03 Apr 2021 10:12:46: 29000000 INFO @ Sat, 03 Apr 2021 10:12:53: 37000000 INFO @ Sat, 03 Apr 2021 10:12:55: 33000000 INFO @ Sat, 03 Apr 2021 10:12:56: 30000000 INFO @ Sat, 03 Apr 2021 10:13:02: 38000000 INFO @ Sat, 03 Apr 2021 10:13:05: 34000000 INFO @ Sat, 03 Apr 2021 10:13:06: 31000000 INFO @ Sat, 03 Apr 2021 10:13:12: 39000000 INFO @ Sat, 03 Apr 2021 10:13:14: 35000000 INFO @ Sat, 03 Apr 2021 10:13:16: 32000000 INFO @ Sat, 03 Apr 2021 10:13:21: 40000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 10:13:24: 36000000 INFO @ Sat, 03 Apr 2021 10:13:25: 33000000 INFO @ Sat, 03 Apr 2021 10:13:31: 41000000 INFO @ Sat, 03 Apr 2021 10:13:33: 37000000 INFO @ Sat, 03 Apr 2021 10:13:35: 34000000 INFO @ Sat, 03 Apr 2021 10:13:40: 42000000 INFO @ Sat, 03 Apr 2021 10:13:43: 38000000 INFO @ Sat, 03 Apr 2021 10:13:45: 35000000 INFO @ Sat, 03 Apr 2021 10:13:50: 43000000 INFO @ Sat, 03 Apr 2021 10:13:52: 39000000 INFO @ Sat, 03 Apr 2021 10:13:55: 36000000 INFO @ Sat, 03 Apr 2021 10:13:59: 44000000 INFO @ Sat, 03 Apr 2021 10:14:01: 40000000 INFO @ Sat, 03 Apr 2021 10:14:04: 37000000 INFO @ Sat, 03 Apr 2021 10:14:09: 45000000 INFO @ Sat, 03 Apr 2021 10:14:11: 41000000 INFO @ Sat, 03 Apr 2021 10:14:14: 38000000 INFO @ Sat, 03 Apr 2021 10:14:19: 46000000 INFO @ Sat, 03 Apr 2021 10:14:20: 42000000 INFO @ Sat, 03 Apr 2021 10:14:23: 39000000 INFO @ Sat, 03 Apr 2021 10:14:29: 47000000 INFO @ Sat, 03 Apr 2021 10:14:29: 43000000 INFO @ Sat, 03 Apr 2021 10:14:33: 40000000 INFO @ Sat, 03 Apr 2021 10:14:39: 48000000 INFO @ Sat, 03 Apr 2021 10:14:39: 44000000 BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 10:14:42: 41000000 INFO @ Sat, 03 Apr 2021 10:14:49: 45000000 INFO @ Sat, 03 Apr 2021 10:14:49: 49000000 INFO @ Sat, 03 Apr 2021 10:14:52: 42000000 INFO @ Sat, 03 Apr 2021 10:14:58: 46000000 INFO @ Sat, 03 Apr 2021 10:14:59: 50000000 INFO @ Sat, 03 Apr 2021 10:15:01: 43000000 INFO @ Sat, 03 Apr 2021 10:15:08: 47000000 INFO @ Sat, 03 Apr 2021 10:15:09: 51000000 INFO @ Sat, 03 Apr 2021 10:15:11: 44000000 INFO @ Sat, 03 Apr 2021 10:15:18: 48000000 INFO @ Sat, 03 Apr 2021 10:15:19: 52000000 INFO @ Sat, 03 Apr 2021 10:15:21: 45000000 INFO @ Sat, 03 Apr 2021 10:15:28: 49000000 INFO @ Sat, 03 Apr 2021 10:15:29: 53000000 INFO @ Sat, 03 Apr 2021 10:15:31: 46000000 INFO @ Sat, 03 Apr 2021 10:15:38: 50000000 INFO @ Sat, 03 Apr 2021 10:15:38: 54000000 INFO @ Sat, 03 Apr 2021 10:15:39: #1 tag size is determined as 150 bps INFO @ Sat, 03 Apr 2021 10:15:39: #1 tag size = 150 INFO @ Sat, 03 Apr 2021 10:15:39: #1 total tags in treatment: 21187081 INFO @ Sat, 03 Apr 2021 10:15:39: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 10:15:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 10:15:39: #1 tags after filtering in treatment: 16609310 INFO @ Sat, 03 Apr 2021 10:15:39: #1 Redundant rate of treatment: 0.22 INFO @ Sat, 03 Apr 2021 10:15:39: #1 finished! INFO @ Sat, 03 Apr 2021 10:15:39: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 10:15:39: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 10:15:40: #2 number of paired peaks: 178 WARNING @ Sat, 03 Apr 2021 10:15:40: Fewer paired peaks (178) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 178 pairs to build model! INFO @ Sat, 03 Apr 2021 10:15:40: start model_add_line... INFO @ Sat, 03 Apr 2021 10:15:41: start X-correlation... INFO @ Sat, 03 Apr 2021 10:15:41: end of X-cor INFO @ Sat, 03 Apr 2021 10:15:41: #2 finished! INFO @ Sat, 03 Apr 2021 10:15:41: #2 predicted fragment length is 159 bps INFO @ Sat, 03 Apr 2021 10:15:41: #2 alternative fragment length(s) may be 159 bps INFO @ Sat, 03 Apr 2021 10:15:41: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX6817547/SRX6817547.05_model.r WARNING @ Sat, 03 Apr 2021 10:15:41: #2 Since the d (159) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 10:15:41: #2 You may need to consider one of the other alternative d(s): 159 WARNING @ Sat, 03 Apr 2021 10:15:41: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 10:15:41: #3 Call peaks... INFO @ Sat, 03 Apr 2021 10:15:41: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 10:15:41: 47000000 INFO @ Sat, 03 Apr 2021 10:15:48: 51000000 INFO @ Sat, 03 Apr 2021 10:15:52: 48000000 INFO @ Sat, 03 Apr 2021 10:15:58: 52000000 INFO @ Sat, 03 Apr 2021 10:16:02: 49000000 INFO @ Sat, 03 Apr 2021 10:16:07: 53000000 INFO @ Sat, 03 Apr 2021 10:16:12: 50000000 INFO @ Sat, 03 Apr 2021 10:16:17: 54000000 INFO @ Sat, 03 Apr 2021 10:16:18: #1 tag size is determined as 150 bps INFO @ Sat, 03 Apr 2021 10:16:18: #1 tag size = 150 INFO @ Sat, 03 Apr 2021 10:16:18: #1 total tags in treatment: 21187081 INFO @ Sat, 03 Apr 2021 10:16:18: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 10:16:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 10:16:18: #1 tags after filtering in treatment: 16609310 INFO @ Sat, 03 Apr 2021 10:16:18: #1 Redundant rate of treatment: 0.22 INFO @ Sat, 03 Apr 2021 10:16:18: #1 finished! INFO @ Sat, 03 Apr 2021 10:16:18: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 10:16:18: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 10:16:19: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 10:16:19: #2 number of paired peaks: 178 WARNING @ Sat, 03 Apr 2021 10:16:19: Fewer paired peaks (178) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 178 pairs to build model! INFO @ Sat, 03 Apr 2021 10:16:19: start model_add_line... INFO @ Sat, 03 Apr 2021 10:16:19: start X-correlation... INFO @ Sat, 03 Apr 2021 10:16:19: end of X-cor INFO @ Sat, 03 Apr 2021 10:16:19: #2 finished! INFO @ Sat, 03 Apr 2021 10:16:19: #2 predicted fragment length is 159 bps INFO @ Sat, 03 Apr 2021 10:16:19: #2 alternative fragment length(s) may be 159 bps INFO @ Sat, 03 Apr 2021 10:16:19: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX6817547/SRX6817547.10_model.r WARNING @ Sat, 03 Apr 2021 10:16:19: #2 Since the d (159) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 10:16:19: #2 You may need to consider one of the other alternative d(s): 159 WARNING @ Sat, 03 Apr 2021 10:16:19: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 10:16:19: #3 Call peaks... INFO @ Sat, 03 Apr 2021 10:16:19: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 10:16:22: 51000000 INFO @ Sat, 03 Apr 2021 10:16:31: 52000000 INFO @ Sat, 03 Apr 2021 10:16:39: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX6817547/SRX6817547.05_peaks.xls INFO @ Sat, 03 Apr 2021 10:16:39: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX6817547/SRX6817547.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 10:16:39: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX6817547/SRX6817547.05_summits.bed INFO @ Sat, 03 Apr 2021 10:16:39: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (7339 records, 4 fields): 9 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 10:16:40: 53000000 INFO @ Sat, 03 Apr 2021 10:16:49: 54000000 INFO @ Sat, 03 Apr 2021 10:16:49: #1 tag size is determined as 150 bps INFO @ Sat, 03 Apr 2021 10:16:49: #1 tag size = 150 INFO @ Sat, 03 Apr 2021 10:16:49: #1 total tags in treatment: 21187081 INFO @ Sat, 03 Apr 2021 10:16:49: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 10:16:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 10:16:50: #1 tags after filtering in treatment: 16609310 INFO @ Sat, 03 Apr 2021 10:16:50: #1 Redundant rate of treatment: 0.22 INFO @ Sat, 03 Apr 2021 10:16:50: #1 finished! INFO @ Sat, 03 Apr 2021 10:16:50: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 10:16:50: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 10:16:51: #2 number of paired peaks: 178 WARNING @ Sat, 03 Apr 2021 10:16:51: Fewer paired peaks (178) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 178 pairs to build model! INFO @ Sat, 03 Apr 2021 10:16:51: start model_add_line... INFO @ Sat, 03 Apr 2021 10:16:51: start X-correlation... INFO @ Sat, 03 Apr 2021 10:16:51: end of X-cor INFO @ Sat, 03 Apr 2021 10:16:51: #2 finished! INFO @ Sat, 03 Apr 2021 10:16:51: #2 predicted fragment length is 159 bps INFO @ Sat, 03 Apr 2021 10:16:51: #2 alternative fragment length(s) may be 159 bps INFO @ Sat, 03 Apr 2021 10:16:51: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX6817547/SRX6817547.20_model.r WARNING @ Sat, 03 Apr 2021 10:16:51: #2 Since the d (159) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 10:16:51: #2 You may need to consider one of the other alternative d(s): 159 WARNING @ Sat, 03 Apr 2021 10:16:51: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 10:16:51: #3 Call peaks... INFO @ Sat, 03 Apr 2021 10:16:51: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 10:16:56: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 10:17:14: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX6817547/SRX6817547.10_peaks.xls INFO @ Sat, 03 Apr 2021 10:17:14: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX6817547/SRX6817547.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 10:17:14: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX6817547/SRX6817547.10_summits.bed INFO @ Sat, 03 Apr 2021 10:17:14: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (2573 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 10:17:29: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 10:17:49: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX6817547/SRX6817547.20_peaks.xls INFO @ Sat, 03 Apr 2021 10:17:49: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX6817547/SRX6817547.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 10:17:49: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX6817547/SRX6817547.20_summits.bed INFO @ Sat, 03 Apr 2021 10:17:49: Done! pass1 - making usageList (12 chroms): 0 millis pass2 - checking and writing primary data (599 records, 4 fields): 2 millis CompletedMACS2peakCalling