Job ID = 12265389 SRX = SRX6817542 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 65861922 spots for SRR10084533/SRR10084533.sra Written 65861922 spots for SRR10084533/SRR10084533.sra fastq に変換しました。 bowtie でマッピング中... Your job 12266987 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 02:22:10 65861922 reads; of these: 65861922 (100.00%) were paired; of these: 35314461 (53.62%) aligned concordantly 0 times 22013032 (33.42%) aligned concordantly exactly 1 time 8534429 (12.96%) aligned concordantly >1 times ---- 35314461 pairs aligned concordantly 0 times; of these: 4215018 (11.94%) aligned discordantly 1 time ---- 31099443 pairs aligned 0 times concordantly or discordantly; of these: 62198886 mates make up the pairs; of these: 55324545 (88.95%) aligned 0 times 1201976 (1.93%) aligned exactly 1 time 5672365 (9.12%) aligned >1 times 58.00% overall alignment rate Time searching: 02:22:11 Overall time: 02:22:11 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 56 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 10256009 / 34384598 = 0.2983 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 09:58:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6817542/SRX6817542.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6817542/SRX6817542.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6817542/SRX6817542.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6817542/SRX6817542.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 09:58:50: #1 read tag files... INFO @ Sat, 03 Apr 2021 09:58:50: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 09:59:01: 1000000 INFO @ Sat, 03 Apr 2021 09:59:11: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 09:59:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6817542/SRX6817542.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6817542/SRX6817542.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6817542/SRX6817542.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6817542/SRX6817542.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 09:59:20: #1 read tag files... INFO @ Sat, 03 Apr 2021 09:59:20: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 09:59:22: 3000000 INFO @ Sat, 03 Apr 2021 09:59:31: 1000000 INFO @ Sat, 03 Apr 2021 09:59:34: 4000000 INFO @ Sat, 03 Apr 2021 09:59:43: 2000000 INFO @ Sat, 03 Apr 2021 09:59:45: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 09:59:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6817542/SRX6817542.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6817542/SRX6817542.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6817542/SRX6817542.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6817542/SRX6817542.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 09:59:50: #1 read tag files... INFO @ Sat, 03 Apr 2021 09:59:50: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 09:59:54: 3000000 INFO @ Sat, 03 Apr 2021 09:59:57: 6000000 INFO @ Sat, 03 Apr 2021 10:00:03: 1000000 INFO @ Sat, 03 Apr 2021 10:00:06: 4000000 INFO @ Sat, 03 Apr 2021 10:00:09: 7000000 INFO @ Sat, 03 Apr 2021 10:00:15: 2000000 INFO @ Sat, 03 Apr 2021 10:00:18: 5000000 INFO @ Sat, 03 Apr 2021 10:00:21: 8000000 INFO @ Sat, 03 Apr 2021 10:00:27: 3000000 INFO @ Sat, 03 Apr 2021 10:00:30: 6000000 INFO @ Sat, 03 Apr 2021 10:00:33: 9000000 INFO @ Sat, 03 Apr 2021 10:00:39: 4000000 INFO @ Sat, 03 Apr 2021 10:00:42: 7000000 INFO @ Sat, 03 Apr 2021 10:00:45: 10000000 INFO @ Sat, 03 Apr 2021 10:00:51: 5000000 INFO @ Sat, 03 Apr 2021 10:00:53: 8000000 INFO @ Sat, 03 Apr 2021 10:00:57: 11000000 INFO @ Sat, 03 Apr 2021 10:01:04: 6000000 INFO @ Sat, 03 Apr 2021 10:01:05: 9000000 INFO @ Sat, 03 Apr 2021 10:01:08: 12000000 INFO @ Sat, 03 Apr 2021 10:01:16: 7000000 INFO @ Sat, 03 Apr 2021 10:01:17: 10000000 INFO @ Sat, 03 Apr 2021 10:01:20: 13000000 INFO @ Sat, 03 Apr 2021 10:01:28: 8000000 INFO @ Sat, 03 Apr 2021 10:01:28: 11000000 INFO @ Sat, 03 Apr 2021 10:01:32: 14000000 INFO @ Sat, 03 Apr 2021 10:01:40: 9000000 INFO @ Sat, 03 Apr 2021 10:01:40: 12000000 INFO @ Sat, 03 Apr 2021 10:01:44: 15000000 INFO @ Sat, 03 Apr 2021 10:01:52: 10000000 INFO @ Sat, 03 Apr 2021 10:01:53: 13000000 INFO @ Sat, 03 Apr 2021 10:01:56: 16000000 INFO @ Sat, 03 Apr 2021 10:02:05: 11000000 INFO @ Sat, 03 Apr 2021 10:02:05: 14000000 INFO @ Sat, 03 Apr 2021 10:02:07: 17000000 INFO @ Sat, 03 Apr 2021 10:02:17: 12000000 INFO @ Sat, 03 Apr 2021 10:02:17: 15000000 INFO @ Sat, 03 Apr 2021 10:02:18: 18000000 INFO @ Sat, 03 Apr 2021 10:02:29: 16000000 INFO @ Sat, 03 Apr 2021 10:02:29: 13000000 INFO @ Sat, 03 Apr 2021 10:02:30: 19000000 INFO @ Sat, 03 Apr 2021 10:02:41: 17000000 INFO @ Sat, 03 Apr 2021 10:02:42: 20000000 INFO @ Sat, 03 Apr 2021 10:02:42: 14000000 INFO @ Sat, 03 Apr 2021 10:02:53: 18000000 INFO @ Sat, 03 Apr 2021 10:02:53: 21000000 INFO @ Sat, 03 Apr 2021 10:02:54: 15000000 INFO @ Sat, 03 Apr 2021 10:03:04: 19000000 INFO @ Sat, 03 Apr 2021 10:03:05: 22000000 INFO @ Sat, 03 Apr 2021 10:03:06: 16000000 INFO @ Sat, 03 Apr 2021 10:03:16: 20000000 INFO @ Sat, 03 Apr 2021 10:03:16: 23000000 INFO @ Sat, 03 Apr 2021 10:03:18: 17000000 INFO @ Sat, 03 Apr 2021 10:03:27: 24000000 INFO @ Sat, 03 Apr 2021 10:03:28: 21000000 INFO @ Sat, 03 Apr 2021 10:03:30: 18000000 INFO @ Sat, 03 Apr 2021 10:03:39: 25000000 INFO @ Sat, 03 Apr 2021 10:03:39: 22000000 INFO @ Sat, 03 Apr 2021 10:03:42: 19000000 INFO @ Sat, 03 Apr 2021 10:03:50: 26000000 INFO @ Sat, 03 Apr 2021 10:03:51: 23000000 INFO @ Sat, 03 Apr 2021 10:03:54: 20000000 INFO @ Sat, 03 Apr 2021 10:04:02: 27000000 INFO @ Sat, 03 Apr 2021 10:04:03: 24000000 INFO @ Sat, 03 Apr 2021 10:04:06: 21000000 INFO @ Sat, 03 Apr 2021 10:04:13: 28000000 INFO @ Sat, 03 Apr 2021 10:04:15: 25000000 INFO @ Sat, 03 Apr 2021 10:04:18: 22000000 INFO @ Sat, 03 Apr 2021 10:04:25: 29000000 INFO @ Sat, 03 Apr 2021 10:04:26: 26000000 INFO @ Sat, 03 Apr 2021 10:04:30: 23000000 INFO @ Sat, 03 Apr 2021 10:04:36: 30000000 INFO @ Sat, 03 Apr 2021 10:04:38: 27000000 INFO @ Sat, 03 Apr 2021 10:04:42: 24000000 INFO @ Sat, 03 Apr 2021 10:04:48: 31000000 INFO @ Sat, 03 Apr 2021 10:04:50: 28000000 INFO @ Sat, 03 Apr 2021 10:04:54: 25000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 10:04:59: 32000000 INFO @ Sat, 03 Apr 2021 10:05:02: 29000000 INFO @ Sat, 03 Apr 2021 10:05:06: 26000000 INFO @ Sat, 03 Apr 2021 10:05:11: 33000000 INFO @ Sat, 03 Apr 2021 10:05:14: 30000000 INFO @ Sat, 03 Apr 2021 10:05:18: 27000000 INFO @ Sat, 03 Apr 2021 10:05:22: 34000000 INFO @ Sat, 03 Apr 2021 10:05:25: 31000000 INFO @ Sat, 03 Apr 2021 10:05:30: 28000000 INFO @ Sat, 03 Apr 2021 10:05:34: 35000000 INFO @ Sat, 03 Apr 2021 10:05:37: 32000000 INFO @ Sat, 03 Apr 2021 10:05:42: 29000000 INFO @ Sat, 03 Apr 2021 10:05:46: 36000000 INFO @ Sat, 03 Apr 2021 10:05:49: 33000000 INFO @ Sat, 03 Apr 2021 10:05:55: 30000000 INFO @ Sat, 03 Apr 2021 10:05:57: 37000000 INFO @ Sat, 03 Apr 2021 10:06:00: 34000000 INFO @ Sat, 03 Apr 2021 10:06:07: 31000000 INFO @ Sat, 03 Apr 2021 10:06:09: 38000000 INFO @ Sat, 03 Apr 2021 10:06:12: 35000000 BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 10:06:19: 32000000 INFO @ Sat, 03 Apr 2021 10:06:20: 39000000 INFO @ Sat, 03 Apr 2021 10:06:23: 36000000 INFO @ Sat, 03 Apr 2021 10:06:31: 33000000 INFO @ Sat, 03 Apr 2021 10:06:32: 40000000 INFO @ Sat, 03 Apr 2021 10:06:35: 37000000 INFO @ Sat, 03 Apr 2021 10:06:43: 34000000 INFO @ Sat, 03 Apr 2021 10:06:43: 41000000 INFO @ Sat, 03 Apr 2021 10:06:46: 38000000 INFO @ Sat, 03 Apr 2021 10:06:55: 42000000 INFO @ Sat, 03 Apr 2021 10:06:55: 35000000 INFO @ Sat, 03 Apr 2021 10:06:58: 39000000 INFO @ Sat, 03 Apr 2021 10:07:07: 43000000 INFO @ Sat, 03 Apr 2021 10:07:07: 36000000 INFO @ Sat, 03 Apr 2021 10:07:10: 40000000 INFO @ Sat, 03 Apr 2021 10:07:18: 44000000 INFO @ Sat, 03 Apr 2021 10:07:19: 37000000 INFO @ Sat, 03 Apr 2021 10:07:21: 41000000 INFO @ Sat, 03 Apr 2021 10:07:30: 45000000 INFO @ Sat, 03 Apr 2021 10:07:30: 38000000 INFO @ Sat, 03 Apr 2021 10:07:33: 42000000 INFO @ Sat, 03 Apr 2021 10:07:42: 46000000 INFO @ Sat, 03 Apr 2021 10:07:43: 39000000 INFO @ Sat, 03 Apr 2021 10:07:45: 43000000 INFO @ Sat, 03 Apr 2021 10:07:54: 47000000 INFO @ Sat, 03 Apr 2021 10:07:55: 40000000 INFO @ Sat, 03 Apr 2021 10:07:56: 44000000 INFO @ Sat, 03 Apr 2021 10:08:06: 48000000 INFO @ Sat, 03 Apr 2021 10:08:07: 41000000 INFO @ Sat, 03 Apr 2021 10:08:08: 45000000 INFO @ Sat, 03 Apr 2021 10:08:18: 49000000 INFO @ Sat, 03 Apr 2021 10:08:19: 42000000 INFO @ Sat, 03 Apr 2021 10:08:21: 46000000 INFO @ Sat, 03 Apr 2021 10:08:30: 50000000 INFO @ Sat, 03 Apr 2021 10:08:32: 43000000 INFO @ Sat, 03 Apr 2021 10:08:33: 47000000 INFO @ Sat, 03 Apr 2021 10:08:43: 51000000 INFO @ Sat, 03 Apr 2021 10:08:44: 44000000 INFO @ Sat, 03 Apr 2021 10:08:45: 48000000 INFO @ Sat, 03 Apr 2021 10:08:54: 52000000 INFO @ Sat, 03 Apr 2021 10:08:57: 45000000 INFO @ Sat, 03 Apr 2021 10:08:57: 49000000 INFO @ Sat, 03 Apr 2021 10:09:06: 53000000 INFO @ Sat, 03 Apr 2021 10:09:09: 46000000 INFO @ Sat, 03 Apr 2021 10:09:10: 50000000 INFO @ Sat, 03 Apr 2021 10:09:18: 54000000 INFO @ Sat, 03 Apr 2021 10:09:22: 51000000 INFO @ Sat, 03 Apr 2021 10:09:22: 47000000 INFO @ Sat, 03 Apr 2021 10:09:29: 55000000 INFO @ Sat, 03 Apr 2021 10:09:33: 52000000 INFO @ Sat, 03 Apr 2021 10:09:34: 48000000 INFO @ Sat, 03 Apr 2021 10:09:40: #1 tag size is determined as 150 bps INFO @ Sat, 03 Apr 2021 10:09:40: #1 tag size = 150 INFO @ Sat, 03 Apr 2021 10:09:40: #1 total tags in treatment: 21348646 INFO @ Sat, 03 Apr 2021 10:09:40: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 10:09:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 10:09:40: #1 tags after filtering in treatment: 16571359 INFO @ Sat, 03 Apr 2021 10:09:40: #1 Redundant rate of treatment: 0.22 INFO @ Sat, 03 Apr 2021 10:09:40: #1 finished! INFO @ Sat, 03 Apr 2021 10:09:40: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 10:09:40: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 10:09:41: #2 number of paired peaks: 155 WARNING @ Sat, 03 Apr 2021 10:09:41: Fewer paired peaks (155) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 155 pairs to build model! INFO @ Sat, 03 Apr 2021 10:09:41: start model_add_line... INFO @ Sat, 03 Apr 2021 10:09:41: start X-correlation... INFO @ Sat, 03 Apr 2021 10:09:41: end of X-cor INFO @ Sat, 03 Apr 2021 10:09:41: #2 finished! INFO @ Sat, 03 Apr 2021 10:09:41: #2 predicted fragment length is 155 bps INFO @ Sat, 03 Apr 2021 10:09:41: #2 alternative fragment length(s) may be 155 bps INFO @ Sat, 03 Apr 2021 10:09:41: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX6817542/SRX6817542.05_model.r WARNING @ Sat, 03 Apr 2021 10:09:41: #2 Since the d (155) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 10:09:41: #2 You may need to consider one of the other alternative d(s): 155 WARNING @ Sat, 03 Apr 2021 10:09:41: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 10:09:41: #3 Call peaks... INFO @ Sat, 03 Apr 2021 10:09:41: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 10:09:45: 53000000 INFO @ Sat, 03 Apr 2021 10:09:47: 49000000 INFO @ Sat, 03 Apr 2021 10:09:56: 54000000 INFO @ Sat, 03 Apr 2021 10:09:59: 50000000 INFO @ Sat, 03 Apr 2021 10:10:08: 55000000 INFO @ Sat, 03 Apr 2021 10:10:11: 51000000 INFO @ Sat, 03 Apr 2021 10:10:18: #1 tag size is determined as 150 bps INFO @ Sat, 03 Apr 2021 10:10:18: #1 tag size = 150 INFO @ Sat, 03 Apr 2021 10:10:18: #1 total tags in treatment: 21348646 INFO @ Sat, 03 Apr 2021 10:10:18: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 10:10:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 10:10:19: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 10:10:19: #1 tags after filtering in treatment: 16571359 INFO @ Sat, 03 Apr 2021 10:10:19: #1 Redundant rate of treatment: 0.22 INFO @ Sat, 03 Apr 2021 10:10:19: #1 finished! INFO @ Sat, 03 Apr 2021 10:10:19: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 10:10:19: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 10:10:20: #2 number of paired peaks: 155 WARNING @ Sat, 03 Apr 2021 10:10:20: Fewer paired peaks (155) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 155 pairs to build model! INFO @ Sat, 03 Apr 2021 10:10:20: start model_add_line... INFO @ Sat, 03 Apr 2021 10:10:20: start X-correlation... INFO @ Sat, 03 Apr 2021 10:10:20: end of X-cor INFO @ Sat, 03 Apr 2021 10:10:20: #2 finished! INFO @ Sat, 03 Apr 2021 10:10:20: #2 predicted fragment length is 155 bps INFO @ Sat, 03 Apr 2021 10:10:20: #2 alternative fragment length(s) may be 155 bps INFO @ Sat, 03 Apr 2021 10:10:20: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX6817542/SRX6817542.10_model.r WARNING @ Sat, 03 Apr 2021 10:10:20: #2 Since the d (155) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 10:10:20: #2 You may need to consider one of the other alternative d(s): 155 WARNING @ Sat, 03 Apr 2021 10:10:20: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 10:10:20: #3 Call peaks... INFO @ Sat, 03 Apr 2021 10:10:20: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 10:10:22: 52000000 INFO @ Sat, 03 Apr 2021 10:10:33: 53000000 INFO @ Sat, 03 Apr 2021 10:10:38: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX6817542/SRX6817542.05_peaks.xls INFO @ Sat, 03 Apr 2021 10:10:38: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX6817542/SRX6817542.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 10:10:38: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX6817542/SRX6817542.05_summits.bed INFO @ Sat, 03 Apr 2021 10:10:38: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (6431 records, 4 fields): 8 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 10:10:44: 54000000 INFO @ Sat, 03 Apr 2021 10:10:54: 55000000 INFO @ Sat, 03 Apr 2021 10:10:57: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 10:11:03: #1 tag size is determined as 150 bps INFO @ Sat, 03 Apr 2021 10:11:03: #1 tag size = 150 INFO @ Sat, 03 Apr 2021 10:11:03: #1 total tags in treatment: 21348646 INFO @ Sat, 03 Apr 2021 10:11:03: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 10:11:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 10:11:03: #1 tags after filtering in treatment: 16571359 INFO @ Sat, 03 Apr 2021 10:11:03: #1 Redundant rate of treatment: 0.22 INFO @ Sat, 03 Apr 2021 10:11:03: #1 finished! INFO @ Sat, 03 Apr 2021 10:11:03: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 10:11:03: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 10:11:05: #2 number of paired peaks: 155 WARNING @ Sat, 03 Apr 2021 10:11:05: Fewer paired peaks (155) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 155 pairs to build model! INFO @ Sat, 03 Apr 2021 10:11:05: start model_add_line... INFO @ Sat, 03 Apr 2021 10:11:05: start X-correlation... INFO @ Sat, 03 Apr 2021 10:11:05: end of X-cor INFO @ Sat, 03 Apr 2021 10:11:05: #2 finished! INFO @ Sat, 03 Apr 2021 10:11:05: #2 predicted fragment length is 155 bps INFO @ Sat, 03 Apr 2021 10:11:05: #2 alternative fragment length(s) may be 155 bps INFO @ Sat, 03 Apr 2021 10:11:05: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX6817542/SRX6817542.20_model.r WARNING @ Sat, 03 Apr 2021 10:11:05: #2 Since the d (155) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 10:11:05: #2 You may need to consider one of the other alternative d(s): 155 WARNING @ Sat, 03 Apr 2021 10:11:05: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 10:11:05: #3 Call peaks... INFO @ Sat, 03 Apr 2021 10:11:05: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 10:11:16: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX6817542/SRX6817542.10_peaks.xls INFO @ Sat, 03 Apr 2021 10:11:16: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX6817542/SRX6817542.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 10:11:16: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX6817542/SRX6817542.10_summits.bed INFO @ Sat, 03 Apr 2021 10:11:16: Done! pass1 - making usageList (15 chroms): 0 millis pass2 - checking and writing primary data (2049 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 10:11:41: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 10:11:59: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX6817542/SRX6817542.20_peaks.xls INFO @ Sat, 03 Apr 2021 10:11:59: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX6817542/SRX6817542.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 10:11:59: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX6817542/SRX6817542.20_summits.bed INFO @ Sat, 03 Apr 2021 10:11:59: Done! pass1 - making usageList (11 chroms): 1 millis pass2 - checking and writing primary data (515 records, 4 fields): 1 millis CompletedMACS2peakCalling