Job ID = 12265387 SRX = SRX6817540 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 31864775 spots for SRR10084531/SRR10084531.sra Written 31864775 spots for SRR10084531/SRR10084531.sra fastq に変換しました。 bowtie でマッピング中... Your job 12265574 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:21:09 31864775 reads; of these: 31864775 (100.00%) were unpaired; of these: 19130205 (60.04%) aligned 0 times 9327538 (29.27%) aligned exactly 1 time 3407032 (10.69%) aligned >1 times 39.96% overall alignment rate Time searching: 00:21:09 Overall time: 00:21:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1876922 / 12734570 = 0.1474 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:14:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6817540/SRX6817540.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6817540/SRX6817540.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6817540/SRX6817540.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6817540/SRX6817540.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:14:20: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:14:20: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:14:30: 1000000 INFO @ Sat, 03 Apr 2021 07:14:39: 2000000 INFO @ Sat, 03 Apr 2021 07:14:48: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:14:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6817540/SRX6817540.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6817540/SRX6817540.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6817540/SRX6817540.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6817540/SRX6817540.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:14:50: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:14:50: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:14:58: 4000000 INFO @ Sat, 03 Apr 2021 07:15:00: 1000000 INFO @ Sat, 03 Apr 2021 07:15:08: 5000000 INFO @ Sat, 03 Apr 2021 07:15:10: 2000000 BedGraph に変換中... INFO @ Sat, 03 Apr 2021 07:15:18: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:15:19: 3000000 INFO @ Sat, 03 Apr 2021 07:15:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6817540/SRX6817540.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6817540/SRX6817540.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6817540/SRX6817540.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6817540/SRX6817540.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:15:20: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:15:20: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:15:29: 7000000 INFO @ Sat, 03 Apr 2021 07:15:29: 4000000 INFO @ Sat, 03 Apr 2021 07:15:29: 1000000 INFO @ Sat, 03 Apr 2021 07:15:38: 5000000 INFO @ Sat, 03 Apr 2021 07:15:38: 2000000 INFO @ Sat, 03 Apr 2021 07:15:39: 8000000 INFO @ Sat, 03 Apr 2021 07:15:47: 6000000 INFO @ Sat, 03 Apr 2021 07:15:47: 3000000 INFO @ Sat, 03 Apr 2021 07:15:49: 9000000 INFO @ Sat, 03 Apr 2021 07:15:56: 7000000 INFO @ Sat, 03 Apr 2021 07:15:56: 4000000 INFO @ Sat, 03 Apr 2021 07:16:00: 10000000 INFO @ Sat, 03 Apr 2021 07:16:05: 5000000 INFO @ Sat, 03 Apr 2021 07:16:05: 8000000 INFO @ Sat, 03 Apr 2021 07:16:08: #1 tag size is determined as 101 bps INFO @ Sat, 03 Apr 2021 07:16:08: #1 tag size = 101 INFO @ Sat, 03 Apr 2021 07:16:08: #1 total tags in treatment: 10857648 INFO @ Sat, 03 Apr 2021 07:16:08: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:16:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:16:08: #1 tags after filtering in treatment: 10857648 INFO @ Sat, 03 Apr 2021 07:16:08: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 07:16:08: #1 finished! INFO @ Sat, 03 Apr 2021 07:16:08: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:16:08: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:16:09: #2 number of paired peaks: 71 WARNING @ Sat, 03 Apr 2021 07:16:09: Too few paired peaks (71) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 07:16:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6817540/SRX6817540.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6817540/SRX6817540.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6817540/SRX6817540.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6817540/SRX6817540.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:16:13: 6000000 INFO @ Sat, 03 Apr 2021 07:16:14: 9000000 INFO @ Sat, 03 Apr 2021 07:16:22: 7000000 INFO @ Sat, 03 Apr 2021 07:16:22: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 07:16:30: #1 tag size is determined as 101 bps INFO @ Sat, 03 Apr 2021 07:16:30: #1 tag size = 101 INFO @ Sat, 03 Apr 2021 07:16:30: #1 total tags in treatment: 10857648 INFO @ Sat, 03 Apr 2021 07:16:30: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:16:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:16:30: #1 tags after filtering in treatment: 10857648 INFO @ Sat, 03 Apr 2021 07:16:30: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 07:16:30: #1 finished! INFO @ Sat, 03 Apr 2021 07:16:30: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:16:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:16:31: #2 number of paired peaks: 71 WARNING @ Sat, 03 Apr 2021 07:16:31: Too few paired peaks (71) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 07:16:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6817540/SRX6817540.10_peaks.narrowPeak: No such file or directory INFO @ Sat, 03 Apr 2021 07:16:31: 8000000 pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6817540/SRX6817540.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6817540/SRX6817540.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6817540/SRX6817540.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:16:39: 9000000 INFO @ Sat, 03 Apr 2021 07:16:47: 10000000 INFO @ Sat, 03 Apr 2021 07:16:53: #1 tag size is determined as 101 bps INFO @ Sat, 03 Apr 2021 07:16:53: #1 tag size = 101 INFO @ Sat, 03 Apr 2021 07:16:53: #1 total tags in treatment: 10857648 INFO @ Sat, 03 Apr 2021 07:16:53: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:16:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:16:53: #1 tags after filtering in treatment: 10857648 INFO @ Sat, 03 Apr 2021 07:16:53: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 07:16:53: #1 finished! INFO @ Sat, 03 Apr 2021 07:16:53: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:16:53: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:16:54: #2 number of paired peaks: 71 WARNING @ Sat, 03 Apr 2021 07:16:54: Too few paired peaks (71) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 07:16:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6817540/SRX6817540.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6817540/SRX6817540.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6817540/SRX6817540.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6817540/SRX6817540.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。