Job ID = 12265373 SRX = SRX6817531 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 47748917 spots for SRR10084522/SRR10084522.sra Written 47748917 spots for SRR10084522/SRR10084522.sra fastq に変換しました。 bowtie でマッピング中... Your job 12267054 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 02:35:55 47748917 reads; of these: 47748917 (100.00%) were paired; of these: 21258926 (44.52%) aligned concordantly 0 times 21222406 (44.45%) aligned concordantly exactly 1 time 5267585 (11.03%) aligned concordantly >1 times ---- 21258926 pairs aligned concordantly 0 times; of these: 6874335 (32.34%) aligned discordantly 1 time ---- 14384591 pairs aligned 0 times concordantly or discordantly; of these: 28769182 mates make up the pairs; of these: 22912033 (79.64%) aligned 0 times 2251229 (7.83%) aligned exactly 1 time 3605920 (12.53%) aligned >1 times 76.01% overall alignment rate Time searching: 02:35:55 Overall time: 02:35:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 56 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 7686102 / 33191063 = 0.2316 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 10:15:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6817531/SRX6817531.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6817531/SRX6817531.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6817531/SRX6817531.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6817531/SRX6817531.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 10:15:00: #1 read tag files... INFO @ Sat, 03 Apr 2021 10:15:00: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 10:15:12: 1000000 INFO @ Sat, 03 Apr 2021 10:15:24: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 10:15:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6817531/SRX6817531.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6817531/SRX6817531.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6817531/SRX6817531.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6817531/SRX6817531.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 10:15:30: #1 read tag files... INFO @ Sat, 03 Apr 2021 10:15:30: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 10:15:34: 3000000 INFO @ Sat, 03 Apr 2021 10:15:41: 1000000 INFO @ Sat, 03 Apr 2021 10:15:44: 4000000 INFO @ Sat, 03 Apr 2021 10:15:52: 2000000 INFO @ Sat, 03 Apr 2021 10:15:54: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 10:16:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6817531/SRX6817531.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6817531/SRX6817531.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6817531/SRX6817531.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6817531/SRX6817531.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 10:16:00: #1 read tag files... INFO @ Sat, 03 Apr 2021 10:16:00: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 10:16:02: 3000000 INFO @ Sat, 03 Apr 2021 10:16:05: 6000000 INFO @ Sat, 03 Apr 2021 10:16:12: 1000000 INFO @ Sat, 03 Apr 2021 10:16:12: 4000000 INFO @ Sat, 03 Apr 2021 10:16:16: 7000000 INFO @ Sat, 03 Apr 2021 10:16:23: 5000000 INFO @ Sat, 03 Apr 2021 10:16:24: 2000000 INFO @ Sat, 03 Apr 2021 10:16:28: 8000000 INFO @ Sat, 03 Apr 2021 10:16:33: 6000000 INFO @ Sat, 03 Apr 2021 10:16:36: 3000000 INFO @ Sat, 03 Apr 2021 10:16:39: 9000000 INFO @ Sat, 03 Apr 2021 10:16:44: 7000000 INFO @ Sat, 03 Apr 2021 10:16:48: 4000000 INFO @ Sat, 03 Apr 2021 10:16:51: 10000000 INFO @ Sat, 03 Apr 2021 10:16:54: 8000000 INFO @ Sat, 03 Apr 2021 10:17:01: 5000000 INFO @ Sat, 03 Apr 2021 10:17:02: 11000000 INFO @ Sat, 03 Apr 2021 10:17:05: 9000000 INFO @ Sat, 03 Apr 2021 10:17:13: 6000000 INFO @ Sat, 03 Apr 2021 10:17:13: 12000000 INFO @ Sat, 03 Apr 2021 10:17:16: 10000000 INFO @ Sat, 03 Apr 2021 10:17:25: 11000000 INFO @ Sat, 03 Apr 2021 10:17:25: 7000000 INFO @ Sat, 03 Apr 2021 10:17:25: 13000000 INFO @ Sat, 03 Apr 2021 10:17:34: 12000000 INFO @ Sat, 03 Apr 2021 10:17:37: 8000000 INFO @ Sat, 03 Apr 2021 10:17:37: 14000000 INFO @ Sat, 03 Apr 2021 10:17:44: 13000000 INFO @ Sat, 03 Apr 2021 10:17:49: 15000000 INFO @ Sat, 03 Apr 2021 10:17:49: 9000000 INFO @ Sat, 03 Apr 2021 10:17:54: 14000000 INFO @ Sat, 03 Apr 2021 10:18:00: 10000000 INFO @ Sat, 03 Apr 2021 10:18:00: 16000000 INFO @ Sat, 03 Apr 2021 10:18:04: 15000000 INFO @ Sat, 03 Apr 2021 10:18:11: 17000000 INFO @ Sat, 03 Apr 2021 10:18:11: 11000000 INFO @ Sat, 03 Apr 2021 10:18:14: 16000000 INFO @ Sat, 03 Apr 2021 10:18:22: 18000000 INFO @ Sat, 03 Apr 2021 10:18:23: 12000000 INFO @ Sat, 03 Apr 2021 10:18:25: 17000000 INFO @ Sat, 03 Apr 2021 10:18:33: 19000000 INFO @ Sat, 03 Apr 2021 10:18:34: 13000000 INFO @ Sat, 03 Apr 2021 10:18:36: 18000000 INFO @ Sat, 03 Apr 2021 10:18:44: 20000000 INFO @ Sat, 03 Apr 2021 10:18:45: 19000000 INFO @ Sat, 03 Apr 2021 10:18:46: 14000000 INFO @ Sat, 03 Apr 2021 10:18:55: 20000000 INFO @ Sat, 03 Apr 2021 10:18:56: 21000000 INFO @ Sat, 03 Apr 2021 10:18:58: 15000000 INFO @ Sat, 03 Apr 2021 10:19:05: 21000000 INFO @ Sat, 03 Apr 2021 10:19:09: 22000000 INFO @ Sat, 03 Apr 2021 10:19:11: 16000000 INFO @ Sat, 03 Apr 2021 10:19:14: 22000000 INFO @ Sat, 03 Apr 2021 10:19:21: 23000000 INFO @ Sat, 03 Apr 2021 10:19:23: 17000000 INFO @ Sat, 03 Apr 2021 10:19:24: 23000000 INFO @ Sat, 03 Apr 2021 10:19:33: 24000000 INFO @ Sat, 03 Apr 2021 10:19:34: 24000000 INFO @ Sat, 03 Apr 2021 10:19:35: 18000000 INFO @ Sat, 03 Apr 2021 10:19:43: 25000000 INFO @ Sat, 03 Apr 2021 10:19:47: 25000000 INFO @ Sat, 03 Apr 2021 10:19:48: 19000000 INFO @ Sat, 03 Apr 2021 10:19:52: 26000000 INFO @ Sat, 03 Apr 2021 10:20:00: 20000000 INFO @ Sat, 03 Apr 2021 10:20:00: 26000000 INFO @ Sat, 03 Apr 2021 10:20:02: 27000000 INFO @ Sat, 03 Apr 2021 10:20:12: 28000000 INFO @ Sat, 03 Apr 2021 10:20:13: 21000000 INFO @ Sat, 03 Apr 2021 10:20:14: 27000000 INFO @ Sat, 03 Apr 2021 10:20:22: 29000000 INFO @ Sat, 03 Apr 2021 10:20:26: 22000000 INFO @ Sat, 03 Apr 2021 10:20:26: 28000000 INFO @ Sat, 03 Apr 2021 10:20:31: 30000000 INFO @ Sat, 03 Apr 2021 10:20:38: 23000000 INFO @ Sat, 03 Apr 2021 10:20:39: 29000000 INFO @ Sat, 03 Apr 2021 10:20:41: 31000000 INFO @ Sat, 03 Apr 2021 10:20:50: 32000000 INFO @ Sat, 03 Apr 2021 10:20:50: 24000000 INFO @ Sat, 03 Apr 2021 10:20:51: 30000000 INFO @ Sat, 03 Apr 2021 10:21:00: 33000000 INFO @ Sat, 03 Apr 2021 10:21:02: 31000000 INFO @ Sat, 03 Apr 2021 10:21:02: 25000000 INFO @ Sat, 03 Apr 2021 10:21:09: 34000000 INFO @ Sat, 03 Apr 2021 10:21:13: 32000000 INFO @ Sat, 03 Apr 2021 10:21:14: 26000000 INFO @ Sat, 03 Apr 2021 10:21:19: 35000000 INFO @ Sat, 03 Apr 2021 10:21:22: 33000000 INFO @ Sat, 03 Apr 2021 10:21:25: 27000000 INFO @ Sat, 03 Apr 2021 10:21:29: 36000000 INFO @ Sat, 03 Apr 2021 10:21:32: 34000000 INFO @ Sat, 03 Apr 2021 10:21:35: 28000000 INFO @ Sat, 03 Apr 2021 10:21:40: 37000000 INFO @ Sat, 03 Apr 2021 10:21:42: 35000000 INFO @ Sat, 03 Apr 2021 10:21:46: 29000000 INFO @ Sat, 03 Apr 2021 10:21:50: 38000000 INFO @ Sat, 03 Apr 2021 10:21:52: 36000000 INFO @ Sat, 03 Apr 2021 10:21:57: 30000000 INFO @ Sat, 03 Apr 2021 10:22:01: 39000000 INFO @ Sat, 03 Apr 2021 10:22:02: 37000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 10:22:07: 31000000 INFO @ Sat, 03 Apr 2021 10:22:13: 40000000 INFO @ Sat, 03 Apr 2021 10:22:13: 38000000 INFO @ Sat, 03 Apr 2021 10:22:18: 32000000 INFO @ Sat, 03 Apr 2021 10:22:23: 39000000 INFO @ Sat, 03 Apr 2021 10:22:25: 41000000 INFO @ Sat, 03 Apr 2021 10:22:28: 33000000 INFO @ Sat, 03 Apr 2021 10:22:33: 40000000 INFO @ Sat, 03 Apr 2021 10:22:38: 42000000 INFO @ Sat, 03 Apr 2021 10:22:38: 34000000 INFO @ Sat, 03 Apr 2021 10:22:43: 41000000 INFO @ Sat, 03 Apr 2021 10:22:49: 35000000 INFO @ Sat, 03 Apr 2021 10:22:52: 43000000 INFO @ Sat, 03 Apr 2021 10:22:54: 42000000 INFO @ Sat, 03 Apr 2021 10:23:00: 36000000 INFO @ Sat, 03 Apr 2021 10:23:04: 44000000 INFO @ Sat, 03 Apr 2021 10:23:04: 43000000 INFO @ Sat, 03 Apr 2021 10:23:12: 37000000 INFO @ Sat, 03 Apr 2021 10:23:15: 44000000 INFO @ Sat, 03 Apr 2021 10:23:17: 45000000 INFO @ Sat, 03 Apr 2021 10:23:24: 38000000 INFO @ Sat, 03 Apr 2021 10:23:26: 45000000 INFO @ Sat, 03 Apr 2021 10:23:30: 46000000 INFO @ Sat, 03 Apr 2021 10:23:36: 39000000 INFO @ Sat, 03 Apr 2021 10:23:37: 46000000 INFO @ Sat, 03 Apr 2021 10:23:42: 47000000 BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 10:23:48: 40000000 INFO @ Sat, 03 Apr 2021 10:23:49: 47000000 INFO @ Sat, 03 Apr 2021 10:23:55: 48000000 INFO @ Sat, 03 Apr 2021 10:24:00: 41000000 INFO @ Sat, 03 Apr 2021 10:24:00: 48000000 INFO @ Sat, 03 Apr 2021 10:24:07: 49000000 INFO @ Sat, 03 Apr 2021 10:24:11: 42000000 INFO @ Sat, 03 Apr 2021 10:24:11: 49000000 INFO @ Sat, 03 Apr 2021 10:24:18: 50000000 INFO @ Sat, 03 Apr 2021 10:24:22: 50000000 INFO @ Sat, 03 Apr 2021 10:24:22: 43000000 INFO @ Sat, 03 Apr 2021 10:24:30: 51000000 INFO @ Sat, 03 Apr 2021 10:24:32: 51000000 INFO @ Sat, 03 Apr 2021 10:24:32: 44000000 INFO @ Sat, 03 Apr 2021 10:24:41: 52000000 INFO @ Sat, 03 Apr 2021 10:24:42: 52000000 INFO @ Sat, 03 Apr 2021 10:24:43: 45000000 INFO @ Sat, 03 Apr 2021 10:24:53: 53000000 INFO @ Sat, 03 Apr 2021 10:24:53: 53000000 INFO @ Sat, 03 Apr 2021 10:24:54: 46000000 INFO @ Sat, 03 Apr 2021 10:25:03: 54000000 INFO @ Sat, 03 Apr 2021 10:25:04: 54000000 INFO @ Sat, 03 Apr 2021 10:25:04: 47000000 INFO @ Sat, 03 Apr 2021 10:25:13: 55000000 INFO @ Sat, 03 Apr 2021 10:25:15: 55000000 INFO @ Sat, 03 Apr 2021 10:25:15: 48000000 INFO @ Sat, 03 Apr 2021 10:25:23: 56000000 INFO @ Sat, 03 Apr 2021 10:25:26: 49000000 INFO @ Sat, 03 Apr 2021 10:25:26: 56000000 INFO @ Sat, 03 Apr 2021 10:25:33: 57000000 INFO @ Sat, 03 Apr 2021 10:25:35: #1 tag size is determined as 150 bps INFO @ Sat, 03 Apr 2021 10:25:35: #1 tag size = 150 INFO @ Sat, 03 Apr 2021 10:25:35: #1 total tags in treatment: 19884951 INFO @ Sat, 03 Apr 2021 10:25:35: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 10:25:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 10:25:36: #1 tags after filtering in treatment: 15386653 INFO @ Sat, 03 Apr 2021 10:25:36: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 03 Apr 2021 10:25:36: #1 finished! INFO @ Sat, 03 Apr 2021 10:25:36: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 10:25:36: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 10:25:36: 50000000 INFO @ Sat, 03 Apr 2021 10:25:37: #2 number of paired peaks: 186 WARNING @ Sat, 03 Apr 2021 10:25:37: Fewer paired peaks (186) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 186 pairs to build model! INFO @ Sat, 03 Apr 2021 10:25:37: start model_add_line... INFO @ Sat, 03 Apr 2021 10:25:37: start X-correlation... INFO @ Sat, 03 Apr 2021 10:25:37: end of X-cor INFO @ Sat, 03 Apr 2021 10:25:37: #2 finished! INFO @ Sat, 03 Apr 2021 10:25:37: #2 predicted fragment length is 198 bps INFO @ Sat, 03 Apr 2021 10:25:37: #2 alternative fragment length(s) may be 198 bps INFO @ Sat, 03 Apr 2021 10:25:37: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX6817531/SRX6817531.05_model.r WARNING @ Sat, 03 Apr 2021 10:25:37: #2 Since the d (198) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 10:25:37: #2 You may need to consider one of the other alternative d(s): 198 WARNING @ Sat, 03 Apr 2021 10:25:37: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 10:25:37: #3 Call peaks... INFO @ Sat, 03 Apr 2021 10:25:37: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 10:25:37: 57000000 INFO @ Sat, 03 Apr 2021 10:25:40: #1 tag size is determined as 150 bps INFO @ Sat, 03 Apr 2021 10:25:40: #1 tag size = 150 INFO @ Sat, 03 Apr 2021 10:25:40: #1 total tags in treatment: 19884951 INFO @ Sat, 03 Apr 2021 10:25:40: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 10:25:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 10:25:40: #1 tags after filtering in treatment: 15386653 INFO @ Sat, 03 Apr 2021 10:25:40: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 03 Apr 2021 10:25:40: #1 finished! INFO @ Sat, 03 Apr 2021 10:25:40: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 10:25:40: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 10:25:41: #2 number of paired peaks: 186 WARNING @ Sat, 03 Apr 2021 10:25:41: Fewer paired peaks (186) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 186 pairs to build model! INFO @ Sat, 03 Apr 2021 10:25:41: start model_add_line... INFO @ Sat, 03 Apr 2021 10:25:42: start X-correlation... INFO @ Sat, 03 Apr 2021 10:25:42: end of X-cor INFO @ Sat, 03 Apr 2021 10:25:42: #2 finished! INFO @ Sat, 03 Apr 2021 10:25:42: #2 predicted fragment length is 198 bps INFO @ Sat, 03 Apr 2021 10:25:42: #2 alternative fragment length(s) may be 198 bps INFO @ Sat, 03 Apr 2021 10:25:42: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX6817531/SRX6817531.10_model.r WARNING @ Sat, 03 Apr 2021 10:25:42: #2 Since the d (198) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 10:25:42: #2 You may need to consider one of the other alternative d(s): 198 WARNING @ Sat, 03 Apr 2021 10:25:42: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 10:25:42: #3 Call peaks... INFO @ Sat, 03 Apr 2021 10:25:42: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 10:25:46: 51000000 INFO @ Sat, 03 Apr 2021 10:25:58: 52000000 INFO @ Sat, 03 Apr 2021 10:26:09: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 10:26:09: 53000000 INFO @ Sat, 03 Apr 2021 10:26:13: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 10:26:20: 54000000 INFO @ Sat, 03 Apr 2021 10:26:26: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX6817531/SRX6817531.05_peaks.xls INFO @ Sat, 03 Apr 2021 10:26:26: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX6817531/SRX6817531.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 10:26:26: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX6817531/SRX6817531.05_summits.bed INFO @ Sat, 03 Apr 2021 10:26:26: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (6393 records, 4 fields): 9 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 10:26:30: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX6817531/SRX6817531.10_peaks.xls INFO @ Sat, 03 Apr 2021 10:26:30: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX6817531/SRX6817531.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 10:26:30: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX6817531/SRX6817531.10_summits.bed INFO @ Sat, 03 Apr 2021 10:26:30: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (2490 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 10:26:31: 55000000 INFO @ Sat, 03 Apr 2021 10:26:42: 56000000 INFO @ Sat, 03 Apr 2021 10:26:53: 57000000 INFO @ Sat, 03 Apr 2021 10:26:55: #1 tag size is determined as 150 bps INFO @ Sat, 03 Apr 2021 10:26:55: #1 tag size = 150 INFO @ Sat, 03 Apr 2021 10:26:55: #1 total tags in treatment: 19884951 INFO @ Sat, 03 Apr 2021 10:26:55: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 10:26:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 10:26:56: #1 tags after filtering in treatment: 15386653 INFO @ Sat, 03 Apr 2021 10:26:56: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 03 Apr 2021 10:26:56: #1 finished! INFO @ Sat, 03 Apr 2021 10:26:56: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 10:26:56: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 10:26:57: #2 number of paired peaks: 186 WARNING @ Sat, 03 Apr 2021 10:26:57: Fewer paired peaks (186) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 186 pairs to build model! INFO @ Sat, 03 Apr 2021 10:26:57: start model_add_line... INFO @ Sat, 03 Apr 2021 10:26:57: start X-correlation... INFO @ Sat, 03 Apr 2021 10:26:57: end of X-cor INFO @ Sat, 03 Apr 2021 10:26:57: #2 finished! INFO @ Sat, 03 Apr 2021 10:26:57: #2 predicted fragment length is 198 bps INFO @ Sat, 03 Apr 2021 10:26:57: #2 alternative fragment length(s) may be 198 bps INFO @ Sat, 03 Apr 2021 10:26:57: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX6817531/SRX6817531.20_model.r WARNING @ Sat, 03 Apr 2021 10:26:57: #2 Since the d (198) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 10:26:57: #2 You may need to consider one of the other alternative d(s): 198 WARNING @ Sat, 03 Apr 2021 10:26:57: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 10:26:57: #3 Call peaks... INFO @ Sat, 03 Apr 2021 10:26:57: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 10:27:29: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 10:27:46: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX6817531/SRX6817531.20_peaks.xls INFO @ Sat, 03 Apr 2021 10:27:46: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX6817531/SRX6817531.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 10:27:46: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX6817531/SRX6817531.20_summits.bed INFO @ Sat, 03 Apr 2021 10:27:46: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (510 records, 4 fields): 2 millis CompletedMACS2peakCalling