Job ID = 6528460 SRX = SRX6747977 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:59:40 prefetch.2.10.7: 1) Downloading 'SRR10009752'... 2020-06-29T14:59:40 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:03:02 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:03:02 prefetch.2.10.7: 1) 'SRR10009752' was downloaded successfully 2020-06-29T15:03:02 prefetch.2.10.7: 'SRR10009752' has 0 unresolved dependencies Read 32104277 spots for SRR10009752/SRR10009752.sra Written 32104277 spots for SRR10009752/SRR10009752.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:32 32104277 reads; of these: 32104277 (100.00%) were unpaired; of these: 664805 (2.07%) aligned 0 times 23678088 (73.75%) aligned exactly 1 time 7761384 (24.18%) aligned >1 times 97.93% overall alignment rate Time searching: 00:12:32 Overall time: 00:12:32 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 2929008 / 31439472 = 0.0932 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:35:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6747977/SRX6747977.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6747977/SRX6747977.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6747977/SRX6747977.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6747977/SRX6747977.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:35:30: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:35:30: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:35:36: 1000000 INFO @ Tue, 30 Jun 2020 00:35:42: 2000000 INFO @ Tue, 30 Jun 2020 00:35:48: 3000000 INFO @ Tue, 30 Jun 2020 00:35:54: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:36:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6747977/SRX6747977.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6747977/SRX6747977.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6747977/SRX6747977.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6747977/SRX6747977.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:36:00: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:36:00: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:36:00: 5000000 INFO @ Tue, 30 Jun 2020 00:36:07: 1000000 INFO @ Tue, 30 Jun 2020 00:36:07: 6000000 INFO @ Tue, 30 Jun 2020 00:36:14: 2000000 INFO @ Tue, 30 Jun 2020 00:36:14: 7000000 INFO @ Tue, 30 Jun 2020 00:36:21: 3000000 INFO @ Tue, 30 Jun 2020 00:36:21: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:36:28: 4000000 INFO @ Tue, 30 Jun 2020 00:36:28: 9000000 INFO @ Tue, 30 Jun 2020 00:36:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6747977/SRX6747977.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6747977/SRX6747977.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6747977/SRX6747977.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6747977/SRX6747977.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:36:30: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:36:30: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:36:35: 5000000 INFO @ Tue, 30 Jun 2020 00:36:35: 10000000 INFO @ Tue, 30 Jun 2020 00:36:37: 1000000 INFO @ Tue, 30 Jun 2020 00:36:43: 11000000 INFO @ Tue, 30 Jun 2020 00:36:43: 6000000 INFO @ Tue, 30 Jun 2020 00:36:45: 2000000 INFO @ Tue, 30 Jun 2020 00:36:50: 12000000 INFO @ Tue, 30 Jun 2020 00:36:50: 7000000 INFO @ Tue, 30 Jun 2020 00:36:52: 3000000 INFO @ Tue, 30 Jun 2020 00:36:57: 13000000 INFO @ Tue, 30 Jun 2020 00:36:58: 8000000 INFO @ Tue, 30 Jun 2020 00:37:00: 4000000 INFO @ Tue, 30 Jun 2020 00:37:04: 14000000 INFO @ Tue, 30 Jun 2020 00:37:05: 9000000 INFO @ Tue, 30 Jun 2020 00:37:07: 5000000 INFO @ Tue, 30 Jun 2020 00:37:12: 15000000 INFO @ Tue, 30 Jun 2020 00:37:12: 10000000 INFO @ Tue, 30 Jun 2020 00:37:15: 6000000 INFO @ Tue, 30 Jun 2020 00:37:19: 16000000 INFO @ Tue, 30 Jun 2020 00:37:20: 11000000 INFO @ Tue, 30 Jun 2020 00:37:22: 7000000 INFO @ Tue, 30 Jun 2020 00:37:26: 17000000 INFO @ Tue, 30 Jun 2020 00:37:27: 12000000 INFO @ Tue, 30 Jun 2020 00:37:29: 8000000 INFO @ Tue, 30 Jun 2020 00:37:33: 18000000 INFO @ Tue, 30 Jun 2020 00:37:34: 13000000 INFO @ Tue, 30 Jun 2020 00:37:36: 9000000 INFO @ Tue, 30 Jun 2020 00:37:40: 19000000 INFO @ Tue, 30 Jun 2020 00:37:41: 14000000 INFO @ Tue, 30 Jun 2020 00:37:44: 10000000 INFO @ Tue, 30 Jun 2020 00:37:47: 20000000 INFO @ Tue, 30 Jun 2020 00:37:48: 15000000 INFO @ Tue, 30 Jun 2020 00:37:51: 11000000 INFO @ Tue, 30 Jun 2020 00:37:55: 21000000 INFO @ Tue, 30 Jun 2020 00:37:55: 16000000 INFO @ Tue, 30 Jun 2020 00:37:58: 12000000 INFO @ Tue, 30 Jun 2020 00:38:02: 22000000 INFO @ Tue, 30 Jun 2020 00:38:02: 17000000 INFO @ Tue, 30 Jun 2020 00:38:05: 13000000 INFO @ Tue, 30 Jun 2020 00:38:09: 18000000 INFO @ Tue, 30 Jun 2020 00:38:09: 23000000 INFO @ Tue, 30 Jun 2020 00:38:13: 14000000 INFO @ Tue, 30 Jun 2020 00:38:16: 19000000 INFO @ Tue, 30 Jun 2020 00:38:17: 24000000 INFO @ Tue, 30 Jun 2020 00:38:20: 15000000 INFO @ Tue, 30 Jun 2020 00:38:23: 20000000 INFO @ Tue, 30 Jun 2020 00:38:24: 25000000 INFO @ Tue, 30 Jun 2020 00:38:27: 16000000 INFO @ Tue, 30 Jun 2020 00:38:30: 21000000 INFO @ Tue, 30 Jun 2020 00:38:31: 26000000 INFO @ Tue, 30 Jun 2020 00:38:34: 17000000 INFO @ Tue, 30 Jun 2020 00:38:38: 22000000 INFO @ Tue, 30 Jun 2020 00:38:39: 27000000 INFO @ Tue, 30 Jun 2020 00:38:41: 18000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:38:45: 23000000 INFO @ Tue, 30 Jun 2020 00:38:46: 28000000 INFO @ Tue, 30 Jun 2020 00:38:48: 19000000 INFO @ Tue, 30 Jun 2020 00:38:50: #1 tag size is determined as 74 bps INFO @ Tue, 30 Jun 2020 00:38:50: #1 tag size = 74 INFO @ Tue, 30 Jun 2020 00:38:50: #1 total tags in treatment: 28510464 INFO @ Tue, 30 Jun 2020 00:38:50: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:38:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:38:50: #1 tags after filtering in treatment: 28510464 INFO @ Tue, 30 Jun 2020 00:38:50: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:38:50: #1 finished! INFO @ Tue, 30 Jun 2020 00:38:50: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:38:50: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:38:52: #2 number of paired peaks: 62 WARNING @ Tue, 30 Jun 2020 00:38:52: Too few paired peaks (62) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:38:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6747977/SRX6747977.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6747977/SRX6747977.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6747977/SRX6747977.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6747977/SRX6747977.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:38:53: 24000000 INFO @ Tue, 30 Jun 2020 00:38:56: 20000000 INFO @ Tue, 30 Jun 2020 00:39:00: 25000000 INFO @ Tue, 30 Jun 2020 00:39:03: 21000000 INFO @ Tue, 30 Jun 2020 00:39:07: 26000000 INFO @ Tue, 30 Jun 2020 00:39:10: 22000000 INFO @ Tue, 30 Jun 2020 00:39:14: 27000000 INFO @ Tue, 30 Jun 2020 00:39:17: 23000000 INFO @ Tue, 30 Jun 2020 00:39:21: 28000000 INFO @ Tue, 30 Jun 2020 00:39:25: 24000000 INFO @ Tue, 30 Jun 2020 00:39:25: #1 tag size is determined as 74 bps INFO @ Tue, 30 Jun 2020 00:39:25: #1 tag size = 74 INFO @ Tue, 30 Jun 2020 00:39:25: #1 total tags in treatment: 28510464 INFO @ Tue, 30 Jun 2020 00:39:25: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:39:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:39:25: #1 tags after filtering in treatment: 28510464 INFO @ Tue, 30 Jun 2020 00:39:25: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:39:25: #1 finished! INFO @ Tue, 30 Jun 2020 00:39:25: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:39:25: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:39:27: #2 number of paired peaks: 62 WARNING @ Tue, 30 Jun 2020 00:39:27: Too few paired peaks (62) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:39:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6747977/SRX6747977.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6747977/SRX6747977.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6747977/SRX6747977.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6747977/SRX6747977.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:39:31: 25000000 INFO @ Tue, 30 Jun 2020 00:39:37: 26000000 INFO @ Tue, 30 Jun 2020 00:39:43: 27000000 BigWig に変換しました。 INFO @ Tue, 30 Jun 2020 00:39:49: 28000000 INFO @ Tue, 30 Jun 2020 00:39:52: #1 tag size is determined as 74 bps INFO @ Tue, 30 Jun 2020 00:39:52: #1 tag size = 74 INFO @ Tue, 30 Jun 2020 00:39:52: #1 total tags in treatment: 28510464 INFO @ Tue, 30 Jun 2020 00:39:52: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:39:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:39:52: #1 tags after filtering in treatment: 28510464 INFO @ Tue, 30 Jun 2020 00:39:52: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:39:52: #1 finished! INFO @ Tue, 30 Jun 2020 00:39:52: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:39:52: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:39:54: #2 number of paired peaks: 62 WARNING @ Tue, 30 Jun 2020 00:39:54: Too few paired peaks (62) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:39:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6747977/SRX6747977.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6747977/SRX6747977.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6747977/SRX6747977.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6747977/SRX6747977.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling