Job ID = 6528458 SRX = SRX661063 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:57:58 prefetch.2.10.7: 1) Downloading 'SRR1524298'... 2020-06-29T14:57:58 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:07:01 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:07:01 prefetch.2.10.7: 1) 'SRR1524298' was downloaded successfully Read 38944779 spots for SRR1524298/SRR1524298.sra Written 38944779 spots for SRR1524298/SRR1524298.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:55 38944779 reads; of these: 38944779 (100.00%) were unpaired; of these: 10641846 (27.33%) aligned 0 times 21157252 (54.33%) aligned exactly 1 time 7145681 (18.35%) aligned >1 times 72.67% overall alignment rate Time searching: 00:13:56 Overall time: 00:13:56 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 5204845 / 28302933 = 0.1839 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:44:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX661063/SRX661063.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX661063/SRX661063.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX661063/SRX661063.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX661063/SRX661063.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:44:33: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:44:33: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:44:40: 1000000 INFO @ Tue, 30 Jun 2020 00:44:46: 2000000 INFO @ Tue, 30 Jun 2020 00:44:52: 3000000 INFO @ Tue, 30 Jun 2020 00:44:59: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:45:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX661063/SRX661063.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX661063/SRX661063.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX661063/SRX661063.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX661063/SRX661063.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:45:03: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:45:03: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:45:05: 5000000 INFO @ Tue, 30 Jun 2020 00:45:10: 1000000 INFO @ Tue, 30 Jun 2020 00:45:11: 6000000 INFO @ Tue, 30 Jun 2020 00:45:16: 2000000 INFO @ Tue, 30 Jun 2020 00:45:18: 7000000 INFO @ Tue, 30 Jun 2020 00:45:23: 3000000 INFO @ Tue, 30 Jun 2020 00:45:24: 8000000 INFO @ Tue, 30 Jun 2020 00:45:29: 4000000 INFO @ Tue, 30 Jun 2020 00:45:30: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:45:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX661063/SRX661063.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX661063/SRX661063.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX661063/SRX661063.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX661063/SRX661063.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:45:34: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:45:34: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:45:35: 5000000 INFO @ Tue, 30 Jun 2020 00:45:37: 10000000 INFO @ Tue, 30 Jun 2020 00:45:40: 1000000 INFO @ Tue, 30 Jun 2020 00:45:42: 6000000 INFO @ Tue, 30 Jun 2020 00:45:43: 11000000 INFO @ Tue, 30 Jun 2020 00:45:47: 2000000 INFO @ Tue, 30 Jun 2020 00:45:48: 7000000 INFO @ Tue, 30 Jun 2020 00:45:50: 12000000 INFO @ Tue, 30 Jun 2020 00:45:53: 3000000 INFO @ Tue, 30 Jun 2020 00:45:55: 8000000 INFO @ Tue, 30 Jun 2020 00:45:56: 13000000 INFO @ Tue, 30 Jun 2020 00:45:59: 4000000 INFO @ Tue, 30 Jun 2020 00:46:01: 9000000 INFO @ Tue, 30 Jun 2020 00:46:03: 14000000 INFO @ Tue, 30 Jun 2020 00:46:06: 5000000 INFO @ Tue, 30 Jun 2020 00:46:07: 10000000 INFO @ Tue, 30 Jun 2020 00:46:09: 15000000 INFO @ Tue, 30 Jun 2020 00:46:12: 6000000 INFO @ Tue, 30 Jun 2020 00:46:14: 11000000 INFO @ Tue, 30 Jun 2020 00:46:16: 16000000 INFO @ Tue, 30 Jun 2020 00:46:19: 7000000 INFO @ Tue, 30 Jun 2020 00:46:20: 12000000 INFO @ Tue, 30 Jun 2020 00:46:22: 17000000 INFO @ Tue, 30 Jun 2020 00:46:25: 8000000 INFO @ Tue, 30 Jun 2020 00:46:27: 13000000 INFO @ Tue, 30 Jun 2020 00:46:29: 18000000 INFO @ Tue, 30 Jun 2020 00:46:32: 9000000 INFO @ Tue, 30 Jun 2020 00:46:33: 14000000 INFO @ Tue, 30 Jun 2020 00:46:36: 19000000 INFO @ Tue, 30 Jun 2020 00:46:38: 10000000 INFO @ Tue, 30 Jun 2020 00:46:40: 15000000 INFO @ Tue, 30 Jun 2020 00:46:42: 20000000 INFO @ Tue, 30 Jun 2020 00:46:45: 11000000 INFO @ Tue, 30 Jun 2020 00:46:47: 16000000 INFO @ Tue, 30 Jun 2020 00:46:49: 21000000 INFO @ Tue, 30 Jun 2020 00:46:51: 12000000 INFO @ Tue, 30 Jun 2020 00:46:53: 17000000 INFO @ Tue, 30 Jun 2020 00:46:55: 22000000 INFO @ Tue, 30 Jun 2020 00:46:58: 13000000 INFO @ Tue, 30 Jun 2020 00:47:00: 18000000 INFO @ Tue, 30 Jun 2020 00:47:02: 23000000 INFO @ Tue, 30 Jun 2020 00:47:02: #1 tag size is determined as 75 bps INFO @ Tue, 30 Jun 2020 00:47:02: #1 tag size = 75 INFO @ Tue, 30 Jun 2020 00:47:02: #1 total tags in treatment: 23098088 INFO @ Tue, 30 Jun 2020 00:47:02: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:47:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:47:03: #1 tags after filtering in treatment: 23098088 INFO @ Tue, 30 Jun 2020 00:47:03: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:47:03: #1 finished! INFO @ Tue, 30 Jun 2020 00:47:03: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:47:03: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:47:04: #2 number of paired peaks: 36 WARNING @ Tue, 30 Jun 2020 00:47:04: Too few paired peaks (36) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:47:04: Process for pairing-model is terminated! INFO @ Tue, 30 Jun 2020 00:47:04: 14000000 cut: /home/okishinya/chipatlas/results/dm3/SRX661063/SRX661063.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX661063/SRX661063.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX661063/SRX661063.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX661063/SRX661063.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:47:06: 19000000 INFO @ Tue, 30 Jun 2020 00:47:11: 15000000 INFO @ Tue, 30 Jun 2020 00:47:13: 20000000 INFO @ Tue, 30 Jun 2020 00:47:18: 16000000 INFO @ Tue, 30 Jun 2020 00:47:19: 21000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:47:24: 17000000 INFO @ Tue, 30 Jun 2020 00:47:26: 22000000 INFO @ Tue, 30 Jun 2020 00:47:31: 18000000 INFO @ Tue, 30 Jun 2020 00:47:32: 23000000 INFO @ Tue, 30 Jun 2020 00:47:33: #1 tag size is determined as 75 bps INFO @ Tue, 30 Jun 2020 00:47:33: #1 tag size = 75 INFO @ Tue, 30 Jun 2020 00:47:33: #1 total tags in treatment: 23098088 INFO @ Tue, 30 Jun 2020 00:47:33: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:47:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:47:33: #1 tags after filtering in treatment: 23098088 INFO @ Tue, 30 Jun 2020 00:47:33: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:47:33: #1 finished! INFO @ Tue, 30 Jun 2020 00:47:33: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:47:33: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:47:35: #2 number of paired peaks: 36 WARNING @ Tue, 30 Jun 2020 00:47:35: Too few paired peaks (36) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:47:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX661063/SRX661063.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX661063/SRX661063.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX661063/SRX661063.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX661063/SRX661063.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:47:38: 19000000 INFO @ Tue, 30 Jun 2020 00:47:44: 20000000 INFO @ Tue, 30 Jun 2020 00:47:51: 21000000 INFO @ Tue, 30 Jun 2020 00:47:57: 22000000 INFO @ Tue, 30 Jun 2020 00:48:04: 23000000 INFO @ Tue, 30 Jun 2020 00:48:05: #1 tag size is determined as 75 bps INFO @ Tue, 30 Jun 2020 00:48:05: #1 tag size = 75 INFO @ Tue, 30 Jun 2020 00:48:05: #1 total tags in treatment: 23098088 INFO @ Tue, 30 Jun 2020 00:48:05: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:48:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:48:05: #1 tags after filtering in treatment: 23098088 INFO @ Tue, 30 Jun 2020 00:48:05: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:48:05: #1 finished! INFO @ Tue, 30 Jun 2020 00:48:05: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:48:05: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:48:06: #2 number of paired peaks: 36 WARNING @ Tue, 30 Jun 2020 00:48:06: Too few paired peaks (36) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:48:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX661063/SRX661063.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX661063/SRX661063.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX661063/SRX661063.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX661063/SRX661063.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。