Job ID = 6528457 SRX = SRX647919 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T15:23:34 prefetch.2.10.7: 1) Downloading 'SRR1508955'... 2020-06-29T15:23:34 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:25:56 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:25:56 prefetch.2.10.7: 1) 'SRR1508955' was downloaded successfully 2020-06-29T15:25:56 prefetch.2.10.7: 'SRR1508955' has 0 unresolved dependencies Read 16412362 spots for SRR1508955/SRR1508955.sra Written 16412362 spots for SRR1508955/SRR1508955.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:17 16412362 reads; of these: 16412362 (100.00%) were unpaired; of these: 610831 (3.72%) aligned 0 times 11922835 (72.65%) aligned exactly 1 time 3878696 (23.63%) aligned >1 times 96.28% overall alignment rate Time searching: 00:05:17 Overall time: 00:05:17 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1920164 / 15801531 = 0.1215 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:40:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX647919/SRX647919.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX647919/SRX647919.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX647919/SRX647919.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX647919/SRX647919.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:40:45: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:40:45: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:40:51: 1000000 INFO @ Tue, 30 Jun 2020 00:40:56: 2000000 INFO @ Tue, 30 Jun 2020 00:41:02: 3000000 INFO @ Tue, 30 Jun 2020 00:41:07: 4000000 INFO @ Tue, 30 Jun 2020 00:41:12: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:41:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX647919/SRX647919.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX647919/SRX647919.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX647919/SRX647919.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX647919/SRX647919.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:41:15: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:41:15: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:41:18: 6000000 INFO @ Tue, 30 Jun 2020 00:41:22: 1000000 INFO @ Tue, 30 Jun 2020 00:41:25: 7000000 INFO @ Tue, 30 Jun 2020 00:41:29: 2000000 INFO @ Tue, 30 Jun 2020 00:41:31: 8000000 INFO @ Tue, 30 Jun 2020 00:41:36: 3000000 INFO @ Tue, 30 Jun 2020 00:41:37: 9000000 INFO @ Tue, 30 Jun 2020 00:41:43: 4000000 INFO @ Tue, 30 Jun 2020 00:41:43: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:41:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX647919/SRX647919.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX647919/SRX647919.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX647919/SRX647919.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX647919/SRX647919.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:41:45: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:41:45: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:41:49: 5000000 INFO @ Tue, 30 Jun 2020 00:41:50: 11000000 INFO @ Tue, 30 Jun 2020 00:41:52: 1000000 INFO @ Tue, 30 Jun 2020 00:41:56: 12000000 INFO @ Tue, 30 Jun 2020 00:41:56: 6000000 INFO @ Tue, 30 Jun 2020 00:41:59: 2000000 INFO @ Tue, 30 Jun 2020 00:42:02: 13000000 INFO @ Tue, 30 Jun 2020 00:42:03: 7000000 INFO @ Tue, 30 Jun 2020 00:42:06: 3000000 INFO @ Tue, 30 Jun 2020 00:42:08: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:42:08: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:42:08: #1 total tags in treatment: 13881367 INFO @ Tue, 30 Jun 2020 00:42:08: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:42:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:42:08: #1 tags after filtering in treatment: 13881367 INFO @ Tue, 30 Jun 2020 00:42:08: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:42:08: #1 finished! INFO @ Tue, 30 Jun 2020 00:42:08: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:42:08: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:42:09: #2 number of paired peaks: 68 WARNING @ Tue, 30 Jun 2020 00:42:09: Too few paired peaks (68) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:42:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX647919/SRX647919.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX647919/SRX647919.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX647919/SRX647919.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX647919/SRX647919.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:42:10: 8000000 INFO @ Tue, 30 Jun 2020 00:42:13: 4000000 INFO @ Tue, 30 Jun 2020 00:42:17: 9000000 INFO @ Tue, 30 Jun 2020 00:42:19: 5000000 INFO @ Tue, 30 Jun 2020 00:42:23: 10000000 INFO @ Tue, 30 Jun 2020 00:42:26: 6000000 INFO @ Tue, 30 Jun 2020 00:42:30: 11000000 INFO @ Tue, 30 Jun 2020 00:42:33: 7000000 INFO @ Tue, 30 Jun 2020 00:42:37: 12000000 INFO @ Tue, 30 Jun 2020 00:42:40: 8000000 INFO @ Tue, 30 Jun 2020 00:42:44: 13000000 INFO @ Tue, 30 Jun 2020 00:42:46: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:42:50: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:42:50: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:42:50: #1 total tags in treatment: 13881367 INFO @ Tue, 30 Jun 2020 00:42:50: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:42:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:42:50: #1 tags after filtering in treatment: 13881367 INFO @ Tue, 30 Jun 2020 00:42:50: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:42:50: #1 finished! INFO @ Tue, 30 Jun 2020 00:42:50: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:42:50: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:42:51: #2 number of paired peaks: 68 WARNING @ Tue, 30 Jun 2020 00:42:51: Too few paired peaks (68) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:42:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX647919/SRX647919.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX647919/SRX647919.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX647919/SRX647919.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX647919/SRX647919.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:42:53: 10000000 INFO @ Tue, 30 Jun 2020 00:43:00: 11000000 INFO @ Tue, 30 Jun 2020 00:43:07: 12000000 INFO @ Tue, 30 Jun 2020 00:43:13: 13000000 INFO @ Tue, 30 Jun 2020 00:43:19: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:43:19: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:43:19: #1 total tags in treatment: 13881367 INFO @ Tue, 30 Jun 2020 00:43:19: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:43:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:43:19: #1 tags after filtering in treatment: 13881367 INFO @ Tue, 30 Jun 2020 00:43:19: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:43:19: #1 finished! INFO @ Tue, 30 Jun 2020 00:43:19: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:43:19: #2 looking for paired plus/minus strand peaks... BigWig に変換しました。 INFO @ Tue, 30 Jun 2020 00:43:20: #2 number of paired peaks: 68 WARNING @ Tue, 30 Jun 2020 00:43:20: Too few paired peaks (68) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:43:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX647919/SRX647919.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX647919/SRX647919.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX647919/SRX647919.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX647919/SRX647919.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling