Job ID = 6528453 SRX = SRX647914 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T15:02:13 prefetch.2.10.7: 1) Downloading 'SRR1508950'... 2020-06-29T15:02:13 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:05:00 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:05:00 prefetch.2.10.7: 1) 'SRR1508950' was downloaded successfully 2020-06-29T15:05:00 prefetch.2.10.7: 'SRR1508950' has 0 unresolved dependencies Read 20149351 spots for SRR1508950/SRR1508950.sra Written 20149351 spots for SRR1508950/SRR1508950.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:13 20149351 reads; of these: 20149351 (100.00%) were unpaired; of these: 826241 (4.10%) aligned 0 times 14806350 (73.48%) aligned exactly 1 time 4516760 (22.42%) aligned >1 times 95.90% overall alignment rate Time searching: 00:06:13 Overall time: 00:06:13 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2257332 / 19323110 = 0.1168 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:22:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX647914/SRX647914.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX647914/SRX647914.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX647914/SRX647914.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX647914/SRX647914.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:22:17: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:22:17: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:22:23: 1000000 INFO @ Tue, 30 Jun 2020 00:22:28: 2000000 INFO @ Tue, 30 Jun 2020 00:22:34: 3000000 INFO @ Tue, 30 Jun 2020 00:22:39: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:22:45: 5000000 INFO @ Tue, 30 Jun 2020 00:22:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX647914/SRX647914.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX647914/SRX647914.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX647914/SRX647914.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX647914/SRX647914.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:22:46: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:22:46: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:22:50: 6000000 INFO @ Tue, 30 Jun 2020 00:22:51: 1000000 INFO @ Tue, 30 Jun 2020 00:22:56: 7000000 INFO @ Tue, 30 Jun 2020 00:22:56: 2000000 INFO @ Tue, 30 Jun 2020 00:23:01: 3000000 INFO @ Tue, 30 Jun 2020 00:23:01: 8000000 INFO @ Tue, 30 Jun 2020 00:23:06: 4000000 INFO @ Tue, 30 Jun 2020 00:23:07: 9000000 INFO @ Tue, 30 Jun 2020 00:23:11: 5000000 INFO @ Tue, 30 Jun 2020 00:23:12: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:23:16: 6000000 INFO @ Tue, 30 Jun 2020 00:23:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX647914/SRX647914.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX647914/SRX647914.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX647914/SRX647914.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX647914/SRX647914.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:23:17: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:23:17: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:23:18: 11000000 INFO @ Tue, 30 Jun 2020 00:23:21: 7000000 INFO @ Tue, 30 Jun 2020 00:23:22: 1000000 INFO @ Tue, 30 Jun 2020 00:23:23: 12000000 INFO @ Tue, 30 Jun 2020 00:23:26: 8000000 INFO @ Tue, 30 Jun 2020 00:23:28: 2000000 INFO @ Tue, 30 Jun 2020 00:23:29: 13000000 INFO @ Tue, 30 Jun 2020 00:23:31: 9000000 INFO @ Tue, 30 Jun 2020 00:23:34: 3000000 INFO @ Tue, 30 Jun 2020 00:23:34: 14000000 INFO @ Tue, 30 Jun 2020 00:23:36: 10000000 INFO @ Tue, 30 Jun 2020 00:23:39: 4000000 INFO @ Tue, 30 Jun 2020 00:23:40: 15000000 INFO @ Tue, 30 Jun 2020 00:23:41: 11000000 INFO @ Tue, 30 Jun 2020 00:23:45: 5000000 INFO @ Tue, 30 Jun 2020 00:23:45: 16000000 INFO @ Tue, 30 Jun 2020 00:23:46: 12000000 INFO @ Tue, 30 Jun 2020 00:23:51: 6000000 INFO @ Tue, 30 Jun 2020 00:23:51: 17000000 INFO @ Tue, 30 Jun 2020 00:23:51: 13000000 INFO @ Tue, 30 Jun 2020 00:23:51: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:23:51: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:23:51: #1 total tags in treatment: 17065778 INFO @ Tue, 30 Jun 2020 00:23:51: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:23:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:23:51: #1 tags after filtering in treatment: 17065778 INFO @ Tue, 30 Jun 2020 00:23:51: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:23:51: #1 finished! INFO @ Tue, 30 Jun 2020 00:23:51: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:23:51: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:23:53: #2 number of paired peaks: 67 WARNING @ Tue, 30 Jun 2020 00:23:53: Too few paired peaks (67) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:23:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX647914/SRX647914.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX647914/SRX647914.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX647914/SRX647914.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX647914/SRX647914.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:23:56: 14000000 INFO @ Tue, 30 Jun 2020 00:23:56: 7000000 INFO @ Tue, 30 Jun 2020 00:24:01: 15000000 INFO @ Tue, 30 Jun 2020 00:24:02: 8000000 INFO @ Tue, 30 Jun 2020 00:24:05: 16000000 INFO @ Tue, 30 Jun 2020 00:24:07: 9000000 INFO @ Tue, 30 Jun 2020 00:24:10: 17000000 INFO @ Tue, 30 Jun 2020 00:24:11: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:24:11: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:24:11: #1 total tags in treatment: 17065778 INFO @ Tue, 30 Jun 2020 00:24:11: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:24:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:24:11: #1 tags after filtering in treatment: 17065778 INFO @ Tue, 30 Jun 2020 00:24:11: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:24:11: #1 finished! INFO @ Tue, 30 Jun 2020 00:24:11: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:24:11: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:24:12: #2 number of paired peaks: 67 WARNING @ Tue, 30 Jun 2020 00:24:12: Too few paired peaks (67) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:24:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX647914/SRX647914.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX647914/SRX647914.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX647914/SRX647914.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX647914/SRX647914.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:24:13: 10000000 INFO @ Tue, 30 Jun 2020 00:24:18: 11000000 INFO @ Tue, 30 Jun 2020 00:24:23: 12000000 INFO @ Tue, 30 Jun 2020 00:24:29: 13000000 INFO @ Tue, 30 Jun 2020 00:24:34: 14000000 INFO @ Tue, 30 Jun 2020 00:24:39: 15000000 INFO @ Tue, 30 Jun 2020 00:24:45: 16000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:24:50: 17000000 INFO @ Tue, 30 Jun 2020 00:24:51: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:24:51: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:24:51: #1 total tags in treatment: 17065778 INFO @ Tue, 30 Jun 2020 00:24:51: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:24:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:24:51: #1 tags after filtering in treatment: 17065778 INFO @ Tue, 30 Jun 2020 00:24:51: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:24:51: #1 finished! INFO @ Tue, 30 Jun 2020 00:24:51: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:24:51: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:24:52: #2 number of paired peaks: 67 WARNING @ Tue, 30 Jun 2020 00:24:52: Too few paired peaks (67) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:24:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX647914/SRX647914.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX647914/SRX647914.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX647914/SRX647914.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX647914/SRX647914.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。