Job ID = 6498641 SRX = SRX647440 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-25T23:55:15 prefetch.2.10.7: 1) Downloading 'SRR1508423'... 2020-06-25T23:55:15 prefetch.2.10.7: Downloading via HTTPS... 2020-06-25T23:58:10 prefetch.2.10.7: HTTPS download succeed 2020-06-25T23:58:10 prefetch.2.10.7: 1) 'SRR1508423' was downloaded successfully Read 19830267 spots for SRR1508423/SRR1508423.sra Written 19830267 spots for SRR1508423/SRR1508423.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:27 19830267 reads; of these: 19830267 (100.00%) were unpaired; of these: 5176971 (26.11%) aligned 0 times 10561807 (53.26%) aligned exactly 1 time 4091489 (20.63%) aligned >1 times 73.89% overall alignment rate Time searching: 00:15:27 Overall time: 00:15:27 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 11692487 / 14653296 = 0.7979 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 09:18:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX647440/SRX647440.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX647440/SRX647440.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX647440/SRX647440.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX647440/SRX647440.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 09:18:51: #1 read tag files... INFO @ Fri, 26 Jun 2020 09:18:51: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 09:18:57: 1000000 INFO @ Fri, 26 Jun 2020 09:19:03: 2000000 INFO @ Fri, 26 Jun 2020 09:19:09: #1 tag size is determined as 100 bps INFO @ Fri, 26 Jun 2020 09:19:09: #1 tag size = 100 INFO @ Fri, 26 Jun 2020 09:19:09: #1 total tags in treatment: 2960809 INFO @ Fri, 26 Jun 2020 09:19:09: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 09:19:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 09:19:09: #1 tags after filtering in treatment: 2960809 INFO @ Fri, 26 Jun 2020 09:19:09: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 09:19:09: #1 finished! INFO @ Fri, 26 Jun 2020 09:19:09: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 09:19:09: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 09:19:10: #2 number of paired peaks: 1696 INFO @ Fri, 26 Jun 2020 09:19:10: start model_add_line... INFO @ Fri, 26 Jun 2020 09:19:10: start X-correlation... INFO @ Fri, 26 Jun 2020 09:19:10: end of X-cor INFO @ Fri, 26 Jun 2020 09:19:10: #2 finished! INFO @ Fri, 26 Jun 2020 09:19:10: #2 predicted fragment length is 84 bps INFO @ Fri, 26 Jun 2020 09:19:10: #2 alternative fragment length(s) may be 84 bps INFO @ Fri, 26 Jun 2020 09:19:10: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX647440/SRX647440.05_model.r WARNING @ Fri, 26 Jun 2020 09:19:10: #2 Since the d (84) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 26 Jun 2020 09:19:10: #2 You may need to consider one of the other alternative d(s): 84 WARNING @ Fri, 26 Jun 2020 09:19:10: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 26 Jun 2020 09:19:10: #3 Call peaks... INFO @ Fri, 26 Jun 2020 09:19:10: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 26 Jun 2020 09:19:16: #3 Call peaks for each chromosome... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 09:19:20: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX647440/SRX647440.05_peaks.xls INFO @ Fri, 26 Jun 2020 09:19:20: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX647440/SRX647440.05_peaks.narrowPeak INFO @ Fri, 26 Jun 2020 09:19:20: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX647440/SRX647440.05_summits.bed INFO @ Fri, 26 Jun 2020 09:19:20: Done! pass1 - making usageList (14 chroms): 1 millis pass2 - checking and writing primary data (2396 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 09:19:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX647440/SRX647440.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX647440/SRX647440.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX647440/SRX647440.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX647440/SRX647440.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 09:19:23: #1 read tag files... INFO @ Fri, 26 Jun 2020 09:19:23: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 09:19:29: 1000000 INFO @ Fri, 26 Jun 2020 09:19:35: 2000000 INFO @ Fri, 26 Jun 2020 09:19:41: #1 tag size is determined as 100 bps INFO @ Fri, 26 Jun 2020 09:19:41: #1 tag size = 100 INFO @ Fri, 26 Jun 2020 09:19:41: #1 total tags in treatment: 2960809 INFO @ Fri, 26 Jun 2020 09:19:41: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 09:19:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 09:19:41: #1 tags after filtering in treatment: 2960809 INFO @ Fri, 26 Jun 2020 09:19:41: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 09:19:41: #1 finished! INFO @ Fri, 26 Jun 2020 09:19:41: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 09:19:41: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 09:19:42: #2 number of paired peaks: 1696 INFO @ Fri, 26 Jun 2020 09:19:42: start model_add_line... INFO @ Fri, 26 Jun 2020 09:19:42: start X-correlation... INFO @ Fri, 26 Jun 2020 09:19:42: end of X-cor INFO @ Fri, 26 Jun 2020 09:19:42: #2 finished! INFO @ Fri, 26 Jun 2020 09:19:42: #2 predicted fragment length is 84 bps INFO @ Fri, 26 Jun 2020 09:19:42: #2 alternative fragment length(s) may be 84 bps INFO @ Fri, 26 Jun 2020 09:19:42: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX647440/SRX647440.10_model.r WARNING @ Fri, 26 Jun 2020 09:19:42: #2 Since the d (84) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 26 Jun 2020 09:19:42: #2 You may need to consider one of the other alternative d(s): 84 WARNING @ Fri, 26 Jun 2020 09:19:42: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 26 Jun 2020 09:19:42: #3 Call peaks... INFO @ Fri, 26 Jun 2020 09:19:42: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 26 Jun 2020 09:19:48: #3 Call peaks for each chromosome... BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 09:19:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX647440/SRX647440.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX647440/SRX647440.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX647440/SRX647440.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX647440/SRX647440.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 09:19:51: #1 read tag files... INFO @ Fri, 26 Jun 2020 09:19:51: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 09:19:51: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX647440/SRX647440.10_peaks.xls INFO @ Fri, 26 Jun 2020 09:19:51: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX647440/SRX647440.10_peaks.narrowPeak INFO @ Fri, 26 Jun 2020 09:19:51: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX647440/SRX647440.10_summits.bed INFO @ Fri, 26 Jun 2020 09:19:51: Done! pass1 - making usageList (14 chroms): 0 millis pass2 - checking and writing primary data (1226 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 09:19:57: 1000000 INFO @ Fri, 26 Jun 2020 09:20:04: 2000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 26 Jun 2020 09:20:10: #1 tag size is determined as 100 bps INFO @ Fri, 26 Jun 2020 09:20:10: #1 tag size = 100 INFO @ Fri, 26 Jun 2020 09:20:10: #1 total tags in treatment: 2960809 INFO @ Fri, 26 Jun 2020 09:20:10: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 09:20:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 09:20:10: #1 tags after filtering in treatment: 2960809 INFO @ Fri, 26 Jun 2020 09:20:10: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 09:20:10: #1 finished! INFO @ Fri, 26 Jun 2020 09:20:10: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 09:20:10: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 09:20:10: #2 number of paired peaks: 1696 INFO @ Fri, 26 Jun 2020 09:20:10: start model_add_line... INFO @ Fri, 26 Jun 2020 09:20:10: start X-correlation... INFO @ Fri, 26 Jun 2020 09:20:10: end of X-cor INFO @ Fri, 26 Jun 2020 09:20:10: #2 finished! INFO @ Fri, 26 Jun 2020 09:20:10: #2 predicted fragment length is 84 bps INFO @ Fri, 26 Jun 2020 09:20:10: #2 alternative fragment length(s) may be 84 bps INFO @ Fri, 26 Jun 2020 09:20:10: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX647440/SRX647440.20_model.r WARNING @ Fri, 26 Jun 2020 09:20:10: #2 Since the d (84) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 26 Jun 2020 09:20:10: #2 You may need to consider one of the other alternative d(s): 84 WARNING @ Fri, 26 Jun 2020 09:20:10: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 26 Jun 2020 09:20:10: #3 Call peaks... INFO @ Fri, 26 Jun 2020 09:20:10: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Fri, 26 Jun 2020 09:20:17: #3 Call peaks for each chromosome... INFO @ Fri, 26 Jun 2020 09:20:20: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX647440/SRX647440.20_peaks.xls INFO @ Fri, 26 Jun 2020 09:20:20: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX647440/SRX647440.20_peaks.narrowPeak INFO @ Fri, 26 Jun 2020 09:20:20: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX647440/SRX647440.20_summits.bed INFO @ Fri, 26 Jun 2020 09:20:20: Done! pass1 - making usageList (10 chroms): 1 millis pass2 - checking and writing primary data (769 records, 4 fields): 2 millis CompletedMACS2peakCalling