Job ID = 6528452 SRX = SRX647439 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:56:28 prefetch.2.10.7: 1) Downloading 'SRR1508422'... 2020-06-29T14:56:28 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:03:18 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:03:18 prefetch.2.10.7: 1) 'SRR1508422' was downloaded successfully Read 35655159 spots for SRR1508422/SRR1508422.sra Written 35655159 spots for SRR1508422/SRR1508422.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:23:54 35655159 reads; of these: 35655159 (100.00%) were unpaired; of these: 2948302 (8.27%) aligned 0 times 23580887 (66.14%) aligned exactly 1 time 9125970 (25.60%) aligned >1 times 91.73% overall alignment rate Time searching: 00:23:54 Overall time: 00:23:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 4060134 / 32706857 = 0.1241 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:58:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX647439/SRX647439.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX647439/SRX647439.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX647439/SRX647439.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX647439/SRX647439.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:58:22: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:58:22: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:58:28: 1000000 INFO @ Tue, 30 Jun 2020 00:58:34: 2000000 INFO @ Tue, 30 Jun 2020 00:58:40: 3000000 INFO @ Tue, 30 Jun 2020 00:58:46: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:58:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX647439/SRX647439.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX647439/SRX647439.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX647439/SRX647439.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX647439/SRX647439.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:58:52: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:58:52: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:58:53: 5000000 INFO @ Tue, 30 Jun 2020 00:58:58: 1000000 INFO @ Tue, 30 Jun 2020 00:58:59: 6000000 INFO @ Tue, 30 Jun 2020 00:59:05: 2000000 INFO @ Tue, 30 Jun 2020 00:59:05: 7000000 INFO @ Tue, 30 Jun 2020 00:59:11: 3000000 INFO @ Tue, 30 Jun 2020 00:59:12: 8000000 INFO @ Tue, 30 Jun 2020 00:59:18: 4000000 INFO @ Tue, 30 Jun 2020 00:59:18: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:59:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX647439/SRX647439.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX647439/SRX647439.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX647439/SRX647439.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX647439/SRX647439.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:59:22: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:59:22: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:59:24: 5000000 INFO @ Tue, 30 Jun 2020 00:59:24: 10000000 INFO @ Tue, 30 Jun 2020 00:59:28: 1000000 INFO @ Tue, 30 Jun 2020 00:59:30: 6000000 INFO @ Tue, 30 Jun 2020 00:59:31: 11000000 INFO @ Tue, 30 Jun 2020 00:59:35: 2000000 INFO @ Tue, 30 Jun 2020 00:59:37: 7000000 INFO @ Tue, 30 Jun 2020 00:59:37: 12000000 INFO @ Tue, 30 Jun 2020 00:59:41: 3000000 INFO @ Tue, 30 Jun 2020 00:59:43: 8000000 INFO @ Tue, 30 Jun 2020 00:59:44: 13000000 INFO @ Tue, 30 Jun 2020 00:59:48: 4000000 INFO @ Tue, 30 Jun 2020 00:59:50: 9000000 INFO @ Tue, 30 Jun 2020 00:59:50: 14000000 INFO @ Tue, 30 Jun 2020 00:59:54: 5000000 INFO @ Tue, 30 Jun 2020 00:59:56: 10000000 INFO @ Tue, 30 Jun 2020 00:59:57: 15000000 INFO @ Tue, 30 Jun 2020 01:00:01: 6000000 INFO @ Tue, 30 Jun 2020 01:00:02: 11000000 INFO @ Tue, 30 Jun 2020 01:00:03: 16000000 INFO @ Tue, 30 Jun 2020 01:00:07: 7000000 INFO @ Tue, 30 Jun 2020 01:00:09: 12000000 INFO @ Tue, 30 Jun 2020 01:00:10: 17000000 INFO @ Tue, 30 Jun 2020 01:00:14: 8000000 INFO @ Tue, 30 Jun 2020 01:00:15: 13000000 INFO @ Tue, 30 Jun 2020 01:00:16: 18000000 INFO @ Tue, 30 Jun 2020 01:00:20: 9000000 INFO @ Tue, 30 Jun 2020 01:00:21: 14000000 INFO @ Tue, 30 Jun 2020 01:00:23: 19000000 INFO @ Tue, 30 Jun 2020 01:00:27: 10000000 INFO @ Tue, 30 Jun 2020 01:00:27: 15000000 INFO @ Tue, 30 Jun 2020 01:00:29: 20000000 INFO @ Tue, 30 Jun 2020 01:00:33: 11000000 INFO @ Tue, 30 Jun 2020 01:00:34: 16000000 INFO @ Tue, 30 Jun 2020 01:00:36: 21000000 INFO @ Tue, 30 Jun 2020 01:00:40: 12000000 INFO @ Tue, 30 Jun 2020 01:00:40: 17000000 INFO @ Tue, 30 Jun 2020 01:00:42: 22000000 INFO @ Tue, 30 Jun 2020 01:00:46: 13000000 INFO @ Tue, 30 Jun 2020 01:00:47: 18000000 INFO @ Tue, 30 Jun 2020 01:00:49: 23000000 INFO @ Tue, 30 Jun 2020 01:00:52: 14000000 INFO @ Tue, 30 Jun 2020 01:00:53: 19000000 INFO @ Tue, 30 Jun 2020 01:00:55: 24000000 INFO @ Tue, 30 Jun 2020 01:00:59: 15000000 INFO @ Tue, 30 Jun 2020 01:00:59: 20000000 INFO @ Tue, 30 Jun 2020 01:01:01: 25000000 INFO @ Tue, 30 Jun 2020 01:01:05: 16000000 INFO @ Tue, 30 Jun 2020 01:01:06: 21000000 INFO @ Tue, 30 Jun 2020 01:01:08: 26000000 INFO @ Tue, 30 Jun 2020 01:01:11: 17000000 INFO @ Tue, 30 Jun 2020 01:01:12: 22000000 INFO @ Tue, 30 Jun 2020 01:01:14: 27000000 INFO @ Tue, 30 Jun 2020 01:01:18: 18000000 INFO @ Tue, 30 Jun 2020 01:01:19: 23000000 INFO @ Tue, 30 Jun 2020 01:01:20: 28000000 INFO @ Tue, 30 Jun 2020 01:01:24: 19000000 INFO @ Tue, 30 Jun 2020 01:01:25: #1 tag size is determined as 100 bps INFO @ Tue, 30 Jun 2020 01:01:25: #1 tag size = 100 INFO @ Tue, 30 Jun 2020 01:01:25: #1 total tags in treatment: 28646723 INFO @ Tue, 30 Jun 2020 01:01:25: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:01:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:01:25: 24000000 INFO @ Tue, 30 Jun 2020 01:01:25: #1 tags after filtering in treatment: 28646723 INFO @ Tue, 30 Jun 2020 01:01:25: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:01:25: #1 finished! INFO @ Tue, 30 Jun 2020 01:01:25: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:01:25: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:01:27: #2 number of paired peaks: 12 WARNING @ Tue, 30 Jun 2020 01:01:27: Too few paired peaks (12) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:01:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX647439/SRX647439.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX647439/SRX647439.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX647439/SRX647439.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX647439/SRX647439.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 01:01:30: 20000000 INFO @ Tue, 30 Jun 2020 01:01:31: 25000000 INFO @ Tue, 30 Jun 2020 01:01:37: 21000000 INFO @ Tue, 30 Jun 2020 01:01:38: 26000000 INFO @ Tue, 30 Jun 2020 01:01:43: 22000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 01:01:44: 27000000 INFO @ Tue, 30 Jun 2020 01:01:49: 23000000 INFO @ Tue, 30 Jun 2020 01:01:50: 28000000 INFO @ Tue, 30 Jun 2020 01:01:54: #1 tag size is determined as 100 bps INFO @ Tue, 30 Jun 2020 01:01:54: #1 tag size = 100 INFO @ Tue, 30 Jun 2020 01:01:54: #1 total tags in treatment: 28646723 INFO @ Tue, 30 Jun 2020 01:01:54: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:01:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:01:55: #1 tags after filtering in treatment: 28646723 INFO @ Tue, 30 Jun 2020 01:01:55: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:01:55: #1 finished! INFO @ Tue, 30 Jun 2020 01:01:55: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:01:55: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:01:56: 24000000 INFO @ Tue, 30 Jun 2020 01:01:57: #2 number of paired peaks: 12 WARNING @ Tue, 30 Jun 2020 01:01:57: Too few paired peaks (12) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:01:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX647439/SRX647439.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX647439/SRX647439.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX647439/SRX647439.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX647439/SRX647439.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 01:02:02: 25000000 INFO @ Tue, 30 Jun 2020 01:02:08: 26000000 INFO @ Tue, 30 Jun 2020 01:02:14: 27000000 INFO @ Tue, 30 Jun 2020 01:02:20: 28000000 INFO @ Tue, 30 Jun 2020 01:02:24: #1 tag size is determined as 100 bps INFO @ Tue, 30 Jun 2020 01:02:24: #1 tag size = 100 INFO @ Tue, 30 Jun 2020 01:02:24: #1 total tags in treatment: 28646723 INFO @ Tue, 30 Jun 2020 01:02:24: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:02:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:02:24: #1 tags after filtering in treatment: 28646723 INFO @ Tue, 30 Jun 2020 01:02:24: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:02:24: #1 finished! INFO @ Tue, 30 Jun 2020 01:02:24: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:02:24: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:02:26: #2 number of paired peaks: 12 WARNING @ Tue, 30 Jun 2020 01:02:26: Too few paired peaks (12) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:02:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX647439/SRX647439.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX647439/SRX647439.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX647439/SRX647439.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX647439/SRX647439.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。