Job ID = 4178629 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 41,449,601 reads read : 41,449,601 reads written : 41,449,601 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:17:13 41449601 reads; of these: 41449601 (100.00%) were unpaired; of these: 5530010 (13.34%) aligned 0 times 24916289 (60.11%) aligned exactly 1 time 11003302 (26.55%) aligned >1 times 86.66% overall alignment rate Time searching: 00:17:14 Overall time: 00:17:14 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 20305243 / 35919591 = 0.5653 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Thu, 05 Dec 2019 14:06:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6468503/SRX6468503.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6468503/SRX6468503.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6468503/SRX6468503.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6468503/SRX6468503.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 05 Dec 2019 14:06:21: #1 read tag files... INFO @ Thu, 05 Dec 2019 14:06:21: #1 read treatment tags... INFO @ Thu, 05 Dec 2019 14:06:29: 1000000 INFO @ Thu, 05 Dec 2019 14:06:37: 2000000 INFO @ Thu, 05 Dec 2019 14:06:44: 3000000 INFO @ Thu, 05 Dec 2019 14:06:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6468503/SRX6468503.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6468503/SRX6468503.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6468503/SRX6468503.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6468503/SRX6468503.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 05 Dec 2019 14:06:51: #1 read tag files... INFO @ Thu, 05 Dec 2019 14:06:51: #1 read treatment tags... INFO @ Thu, 05 Dec 2019 14:06:52: 4000000 INFO @ Thu, 05 Dec 2019 14:07:00: 5000000 INFO @ Thu, 05 Dec 2019 14:07:00: 1000000 INFO @ Thu, 05 Dec 2019 14:07:08: 6000000 INFO @ Thu, 05 Dec 2019 14:07:10: 2000000 INFO @ Thu, 05 Dec 2019 14:07:16: 7000000 BedGraph に変換中... INFO @ Thu, 05 Dec 2019 14:07:20: 3000000 INFO @ Thu, 05 Dec 2019 14:07:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6468503/SRX6468503.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6468503/SRX6468503.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6468503/SRX6468503.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6468503/SRX6468503.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 05 Dec 2019 14:07:21: #1 read tag files... INFO @ Thu, 05 Dec 2019 14:07:21: #1 read treatment tags... INFO @ Thu, 05 Dec 2019 14:07:24: 8000000 INFO @ Thu, 05 Dec 2019 14:07:31: 4000000 INFO @ Thu, 05 Dec 2019 14:07:32: 9000000 INFO @ Thu, 05 Dec 2019 14:07:33: 1000000 INFO @ Thu, 05 Dec 2019 14:07:40: 10000000 INFO @ Thu, 05 Dec 2019 14:07:40: 5000000 INFO @ Thu, 05 Dec 2019 14:07:45: 2000000 INFO @ Thu, 05 Dec 2019 14:07:48: 11000000 INFO @ Thu, 05 Dec 2019 14:07:50: 6000000 INFO @ Thu, 05 Dec 2019 14:07:56: 12000000 INFO @ Thu, 05 Dec 2019 14:07:57: 3000000 INFO @ Thu, 05 Dec 2019 14:08:00: 7000000 INFO @ Thu, 05 Dec 2019 14:08:04: 13000000 INFO @ Thu, 05 Dec 2019 14:08:09: 8000000 INFO @ Thu, 05 Dec 2019 14:08:10: 4000000 INFO @ Thu, 05 Dec 2019 14:08:12: 14000000 INFO @ Thu, 05 Dec 2019 14:08:18: 9000000 INFO @ Thu, 05 Dec 2019 14:08:20: 15000000 INFO @ Thu, 05 Dec 2019 14:08:23: 5000000 INFO @ Thu, 05 Dec 2019 14:08:24: #1 tag size is determined as 51 bps INFO @ Thu, 05 Dec 2019 14:08:24: #1 tag size = 51 INFO @ Thu, 05 Dec 2019 14:08:24: #1 total tags in treatment: 15614348 INFO @ Thu, 05 Dec 2019 14:08:24: #1 user defined the maximum tags... INFO @ Thu, 05 Dec 2019 14:08:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 05 Dec 2019 14:08:25: #1 tags after filtering in treatment: 15614348 INFO @ Thu, 05 Dec 2019 14:08:25: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 05 Dec 2019 14:08:25: #1 finished! INFO @ Thu, 05 Dec 2019 14:08:25: #2 Build Peak Model... INFO @ Thu, 05 Dec 2019 14:08:25: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 05 Dec 2019 14:08:26: #2 number of paired peaks: 53 WARNING @ Thu, 05 Dec 2019 14:08:26: Too few paired peaks (53) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 05 Dec 2019 14:08:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6468503/SRX6468503.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468503/SRX6468503.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468503/SRX6468503.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468503/SRX6468503.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 05 Dec 2019 14:08:27: 10000000 INFO @ Thu, 05 Dec 2019 14:08:36: 11000000 INFO @ Thu, 05 Dec 2019 14:08:36: 6000000 INFO @ Thu, 05 Dec 2019 14:08:45: 12000000 INFO @ Thu, 05 Dec 2019 14:08:50: 7000000 INFO @ Thu, 05 Dec 2019 14:08:54: 13000000 INFO @ Thu, 05 Dec 2019 14:09:01: 8000000 INFO @ Thu, 05 Dec 2019 14:09:03: 14000000 INFO @ Thu, 05 Dec 2019 14:09:12: 9000000 INFO @ Thu, 05 Dec 2019 14:09:12: 15000000 INFO @ Thu, 05 Dec 2019 14:09:18: #1 tag size is determined as 51 bps INFO @ Thu, 05 Dec 2019 14:09:18: #1 tag size = 51 INFO @ Thu, 05 Dec 2019 14:09:18: #1 total tags in treatment: 15614348 INFO @ Thu, 05 Dec 2019 14:09:18: #1 user defined the maximum tags... INFO @ Thu, 05 Dec 2019 14:09:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 05 Dec 2019 14:09:18: #1 tags after filtering in treatment: 15614348 INFO @ Thu, 05 Dec 2019 14:09:18: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 05 Dec 2019 14:09:18: #1 finished! INFO @ Thu, 05 Dec 2019 14:09:18: #2 Build Peak Model... INFO @ Thu, 05 Dec 2019 14:09:18: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 05 Dec 2019 14:09:19: #2 number of paired peaks: 53 WARNING @ Thu, 05 Dec 2019 14:09:19: Too few paired peaks (53) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 05 Dec 2019 14:09:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6468503/SRX6468503.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468503/SRX6468503.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468503/SRX6468503.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468503/SRX6468503.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 05 Dec 2019 14:09:23: 10000000 INFO @ Thu, 05 Dec 2019 14:09:33: 11000000 INFO @ Thu, 05 Dec 2019 14:09:44: 12000000 INFO @ Thu, 05 Dec 2019 14:09:54: 13000000 INFO @ Thu, 05 Dec 2019 14:10:04: 14000000 INFO @ Thu, 05 Dec 2019 14:10:15: 15000000 INFO @ Thu, 05 Dec 2019 14:10:21: #1 tag size is determined as 51 bps INFO @ Thu, 05 Dec 2019 14:10:21: #1 tag size = 51 INFO @ Thu, 05 Dec 2019 14:10:21: #1 total tags in treatment: 15614348 INFO @ Thu, 05 Dec 2019 14:10:21: #1 user defined the maximum tags... INFO @ Thu, 05 Dec 2019 14:10:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 05 Dec 2019 14:10:21: #1 tags after filtering in treatment: 15614348 INFO @ Thu, 05 Dec 2019 14:10:21: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 05 Dec 2019 14:10:21: #1 finished! INFO @ Thu, 05 Dec 2019 14:10:21: #2 Build Peak Model... INFO @ Thu, 05 Dec 2019 14:10:21: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 05 Dec 2019 14:10:23: #2 number of paired peaks: 53 WARNING @ Thu, 05 Dec 2019 14:10:23: Too few paired peaks (53) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 05 Dec 2019 14:10:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6468503/SRX6468503.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468503/SRX6468503.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468503/SRX6468503.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468503/SRX6468503.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。