Job ID = 6528441 SRX = SRX6468485 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T15:00:28 prefetch.2.10.7: 1) Downloading 'SRR9710571'... 2020-06-29T15:00:28 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:05:20 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:05:20 prefetch.2.10.7: 1) 'SRR9710571' was downloaded successfully Read 29263938 spots for SRR9710571/SRR9710571.sra Written 29263938 spots for SRR9710571/SRR9710571.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:18 29263938 reads; of these: 29263938 (100.00%) were unpaired; of these: 1784068 (6.10%) aligned 0 times 22457257 (76.74%) aligned exactly 1 time 5022613 (17.16%) aligned >1 times 93.90% overall alignment rate Time searching: 00:06:18 Overall time: 00:06:18 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 11921812 / 27479870 = 0.4338 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:25:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6468485/SRX6468485.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6468485/SRX6468485.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6468485/SRX6468485.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6468485/SRX6468485.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:25:32: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:25:32: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:25:38: 1000000 INFO @ Tue, 30 Jun 2020 00:25:44: 2000000 INFO @ Tue, 30 Jun 2020 00:25:49: 3000000 INFO @ Tue, 30 Jun 2020 00:25:55: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:26:01: 5000000 INFO @ Tue, 30 Jun 2020 00:26:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6468485/SRX6468485.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6468485/SRX6468485.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6468485/SRX6468485.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6468485/SRX6468485.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:26:02: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:26:02: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:26:07: 6000000 INFO @ Tue, 30 Jun 2020 00:26:09: 1000000 INFO @ Tue, 30 Jun 2020 00:26:14: 7000000 INFO @ Tue, 30 Jun 2020 00:26:16: 2000000 INFO @ Tue, 30 Jun 2020 00:26:21: 8000000 INFO @ Tue, 30 Jun 2020 00:26:23: 3000000 INFO @ Tue, 30 Jun 2020 00:26:28: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:26:30: 4000000 INFO @ Tue, 30 Jun 2020 00:26:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6468485/SRX6468485.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6468485/SRX6468485.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6468485/SRX6468485.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6468485/SRX6468485.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:26:32: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:26:32: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:26:34: 10000000 INFO @ Tue, 30 Jun 2020 00:26:37: 5000000 INFO @ Tue, 30 Jun 2020 00:26:39: 1000000 INFO @ Tue, 30 Jun 2020 00:26:41: 11000000 INFO @ Tue, 30 Jun 2020 00:26:44: 6000000 INFO @ Tue, 30 Jun 2020 00:26:47: 2000000 INFO @ Tue, 30 Jun 2020 00:26:48: 12000000 INFO @ Tue, 30 Jun 2020 00:26:51: 7000000 INFO @ Tue, 30 Jun 2020 00:26:54: 3000000 INFO @ Tue, 30 Jun 2020 00:26:55: 13000000 INFO @ Tue, 30 Jun 2020 00:26:59: 8000000 INFO @ Tue, 30 Jun 2020 00:27:02: 14000000 INFO @ Tue, 30 Jun 2020 00:27:02: 4000000 INFO @ Tue, 30 Jun 2020 00:27:06: 9000000 INFO @ Tue, 30 Jun 2020 00:27:09: 15000000 INFO @ Tue, 30 Jun 2020 00:27:10: 5000000 INFO @ Tue, 30 Jun 2020 00:27:13: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:27:13: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:27:13: #1 total tags in treatment: 15558058 INFO @ Tue, 30 Jun 2020 00:27:13: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:27:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:27:13: 10000000 INFO @ Tue, 30 Jun 2020 00:27:13: #1 tags after filtering in treatment: 15558058 INFO @ Tue, 30 Jun 2020 00:27:13: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:27:13: #1 finished! INFO @ Tue, 30 Jun 2020 00:27:13: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:27:13: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:27:14: #2 number of paired peaks: 8 WARNING @ Tue, 30 Jun 2020 00:27:14: Too few paired peaks (8) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:27:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6468485/SRX6468485.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468485/SRX6468485.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468485/SRX6468485.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468485/SRX6468485.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:27:17: 6000000 INFO @ Tue, 30 Jun 2020 00:27:21: 11000000 INFO @ Tue, 30 Jun 2020 00:27:25: 7000000 INFO @ Tue, 30 Jun 2020 00:27:28: 12000000 INFO @ Tue, 30 Jun 2020 00:27:33: 8000000 INFO @ Tue, 30 Jun 2020 00:27:35: 13000000 INFO @ Tue, 30 Jun 2020 00:27:41: 9000000 INFO @ Tue, 30 Jun 2020 00:27:42: 14000000 INFO @ Tue, 30 Jun 2020 00:27:49: 10000000 INFO @ Tue, 30 Jun 2020 00:27:50: 15000000 INFO @ Tue, 30 Jun 2020 00:27:54: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:27:54: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:27:54: #1 total tags in treatment: 15558058 INFO @ Tue, 30 Jun 2020 00:27:54: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:27:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:27:54: #1 tags after filtering in treatment: 15558058 INFO @ Tue, 30 Jun 2020 00:27:54: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:27:54: #1 finished! INFO @ Tue, 30 Jun 2020 00:27:54: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:27:54: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:27:55: #2 number of paired peaks: 8 WARNING @ Tue, 30 Jun 2020 00:27:55: Too few paired peaks (8) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:27:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6468485/SRX6468485.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468485/SRX6468485.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468485/SRX6468485.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468485/SRX6468485.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:27:56: 11000000 INFO @ Tue, 30 Jun 2020 00:28:02: 12000000 INFO @ Tue, 30 Jun 2020 00:28:09: 13000000 INFO @ Tue, 30 Jun 2020 00:28:16: 14000000 INFO @ Tue, 30 Jun 2020 00:28:23: 15000000 INFO @ Tue, 30 Jun 2020 00:28:27: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:28:27: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:28:27: #1 total tags in treatment: 15558058 INFO @ Tue, 30 Jun 2020 00:28:27: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:28:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:28:27: #1 tags after filtering in treatment: 15558058 INFO @ Tue, 30 Jun 2020 00:28:27: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:28:27: #1 finished! INFO @ Tue, 30 Jun 2020 00:28:27: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:28:27: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:28:28: #2 number of paired peaks: 8 WARNING @ Tue, 30 Jun 2020 00:28:28: Too few paired peaks (8) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:28:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6468485/SRX6468485.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468485/SRX6468485.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468485/SRX6468485.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468485/SRX6468485.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。