Job ID = 6528439 SRX = SRX645136 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T15:32:54 prefetch.2.10.7: 1) Downloading 'SRR1505736'... 2020-06-29T15:32:54 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:45:27 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:45:27 prefetch.2.10.7: 1) 'SRR1505736' was downloaded successfully Read 61845339 spots for SRR1505736/SRR1505736.sra Written 61845339 spots for SRR1505736/SRR1505736.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:31:21 61845339 reads; of these: 61845339 (100.00%) were unpaired; of these: 30417495 (49.18%) aligned 0 times 23263494 (37.62%) aligned exactly 1 time 8164350 (13.20%) aligned >1 times 50.82% overall alignment rate Time searching: 00:31:21 Overall time: 00:31:21 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 3328412 / 31427844 = 0.1059 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 02:15:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX645136/SRX645136.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX645136/SRX645136.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX645136/SRX645136.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX645136/SRX645136.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 02:15:23: #1 read tag files... INFO @ Tue, 30 Jun 2020 02:15:23: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 02:15:30: 1000000 INFO @ Tue, 30 Jun 2020 02:15:38: 2000000 INFO @ Tue, 30 Jun 2020 02:15:45: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 02:15:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX645136/SRX645136.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX645136/SRX645136.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX645136/SRX645136.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX645136/SRX645136.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 02:15:53: #1 read tag files... INFO @ Tue, 30 Jun 2020 02:15:53: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 02:15:53: 4000000 INFO @ Tue, 30 Jun 2020 02:16:01: 5000000 INFO @ Tue, 30 Jun 2020 02:16:01: 1000000 INFO @ Tue, 30 Jun 2020 02:16:10: 6000000 INFO @ Tue, 30 Jun 2020 02:16:10: 2000000 INFO @ Tue, 30 Jun 2020 02:16:18: 7000000 INFO @ Tue, 30 Jun 2020 02:16:18: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 02:16:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX645136/SRX645136.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX645136/SRX645136.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX645136/SRX645136.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX645136/SRX645136.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 02:16:23: #1 read tag files... INFO @ Tue, 30 Jun 2020 02:16:23: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 02:16:27: 8000000 INFO @ Tue, 30 Jun 2020 02:16:27: 4000000 INFO @ Tue, 30 Jun 2020 02:16:32: 1000000 INFO @ Tue, 30 Jun 2020 02:16:36: 5000000 INFO @ Tue, 30 Jun 2020 02:16:36: 9000000 INFO @ Tue, 30 Jun 2020 02:16:41: 2000000 INFO @ Tue, 30 Jun 2020 02:16:45: 6000000 INFO @ Tue, 30 Jun 2020 02:16:45: 10000000 INFO @ Tue, 30 Jun 2020 02:16:50: 3000000 INFO @ Tue, 30 Jun 2020 02:16:54: 7000000 INFO @ Tue, 30 Jun 2020 02:16:54: 11000000 INFO @ Tue, 30 Jun 2020 02:16:59: 4000000 INFO @ Tue, 30 Jun 2020 02:17:03: 8000000 INFO @ Tue, 30 Jun 2020 02:17:03: 12000000 INFO @ Tue, 30 Jun 2020 02:17:08: 5000000 INFO @ Tue, 30 Jun 2020 02:17:12: 9000000 INFO @ Tue, 30 Jun 2020 02:17:12: 13000000 INFO @ Tue, 30 Jun 2020 02:17:17: 6000000 INFO @ Tue, 30 Jun 2020 02:17:21: 10000000 INFO @ Tue, 30 Jun 2020 02:17:21: 14000000 INFO @ Tue, 30 Jun 2020 02:17:26: 7000000 INFO @ Tue, 30 Jun 2020 02:17:30: 11000000 INFO @ Tue, 30 Jun 2020 02:17:30: 15000000 INFO @ Tue, 30 Jun 2020 02:17:35: 8000000 INFO @ Tue, 30 Jun 2020 02:17:39: 16000000 INFO @ Tue, 30 Jun 2020 02:17:39: 12000000 INFO @ Tue, 30 Jun 2020 02:17:44: 9000000 INFO @ Tue, 30 Jun 2020 02:17:48: 17000000 INFO @ Tue, 30 Jun 2020 02:17:48: 13000000 INFO @ Tue, 30 Jun 2020 02:17:53: 10000000 INFO @ Tue, 30 Jun 2020 02:17:57: 18000000 INFO @ Tue, 30 Jun 2020 02:17:57: 14000000 INFO @ Tue, 30 Jun 2020 02:18:02: 11000000 INFO @ Tue, 30 Jun 2020 02:18:05: 19000000 INFO @ Tue, 30 Jun 2020 02:18:06: 15000000 INFO @ Tue, 30 Jun 2020 02:18:11: 12000000 INFO @ Tue, 30 Jun 2020 02:18:14: 20000000 INFO @ Tue, 30 Jun 2020 02:18:15: 16000000 INFO @ Tue, 30 Jun 2020 02:18:20: 13000000 INFO @ Tue, 30 Jun 2020 02:18:23: 21000000 INFO @ Tue, 30 Jun 2020 02:18:24: 17000000 INFO @ Tue, 30 Jun 2020 02:18:28: 14000000 INFO @ Tue, 30 Jun 2020 02:18:32: 22000000 INFO @ Tue, 30 Jun 2020 02:18:32: 18000000 INFO @ Tue, 30 Jun 2020 02:18:37: 15000000 INFO @ Tue, 30 Jun 2020 02:18:40: 23000000 INFO @ Tue, 30 Jun 2020 02:18:41: 19000000 INFO @ Tue, 30 Jun 2020 02:18:46: 16000000 INFO @ Tue, 30 Jun 2020 02:18:49: 24000000 INFO @ Tue, 30 Jun 2020 02:18:50: 20000000 INFO @ Tue, 30 Jun 2020 02:18:54: 17000000 INFO @ Tue, 30 Jun 2020 02:18:58: 25000000 INFO @ Tue, 30 Jun 2020 02:18:58: 21000000 INFO @ Tue, 30 Jun 2020 02:19:03: 18000000 INFO @ Tue, 30 Jun 2020 02:19:06: 26000000 INFO @ Tue, 30 Jun 2020 02:19:07: 22000000 INFO @ Tue, 30 Jun 2020 02:19:11: 19000000 INFO @ Tue, 30 Jun 2020 02:19:15: 27000000 INFO @ Tue, 30 Jun 2020 02:19:16: 23000000 INFO @ Tue, 30 Jun 2020 02:19:20: 20000000 INFO @ Tue, 30 Jun 2020 02:19:23: 28000000 INFO @ Tue, 30 Jun 2020 02:19:24: #1 tag size is determined as 100 bps INFO @ Tue, 30 Jun 2020 02:19:24: #1 tag size = 100 INFO @ Tue, 30 Jun 2020 02:19:24: #1 total tags in treatment: 28099432 INFO @ Tue, 30 Jun 2020 02:19:24: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 02:19:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 02:19:25: 24000000 INFO @ Tue, 30 Jun 2020 02:19:25: #1 tags after filtering in treatment: 28099432 INFO @ Tue, 30 Jun 2020 02:19:25: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 02:19:25: #1 finished! INFO @ Tue, 30 Jun 2020 02:19:25: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 02:19:25: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 02:19:27: #2 number of paired peaks: 4 WARNING @ Tue, 30 Jun 2020 02:19:27: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 02:19:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX645136/SRX645136.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX645136/SRX645136.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX645136/SRX645136.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX645136/SRX645136.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 02:19:28: 21000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 02:19:33: 25000000 INFO @ Tue, 30 Jun 2020 02:19:37: 22000000 INFO @ Tue, 30 Jun 2020 02:19:41: 26000000 INFO @ Tue, 30 Jun 2020 02:19:45: 23000000 INFO @ Tue, 30 Jun 2020 02:19:49: 27000000 INFO @ Tue, 30 Jun 2020 02:19:53: 24000000 INFO @ Tue, 30 Jun 2020 02:19:57: 28000000 INFO @ Tue, 30 Jun 2020 02:19:58: #1 tag size is determined as 100 bps INFO @ Tue, 30 Jun 2020 02:19:58: #1 tag size = 100 INFO @ Tue, 30 Jun 2020 02:19:58: #1 total tags in treatment: 28099432 INFO @ Tue, 30 Jun 2020 02:19:58: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 02:19:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 02:19:59: #1 tags after filtering in treatment: 28099432 INFO @ Tue, 30 Jun 2020 02:19:59: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 02:19:59: #1 finished! INFO @ Tue, 30 Jun 2020 02:19:59: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 02:19:59: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 02:20:01: #2 number of paired peaks: 4 WARNING @ Tue, 30 Jun 2020 02:20:01: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 02:20:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX645136/SRX645136.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX645136/SRX645136.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX645136/SRX645136.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX645136/SRX645136.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 02:20:01: 25000000 INFO @ Tue, 30 Jun 2020 02:20:09: 26000000 INFO @ Tue, 30 Jun 2020 02:20:16: 27000000 INFO @ Tue, 30 Jun 2020 02:20:23: 28000000 INFO @ Tue, 30 Jun 2020 02:20:24: #1 tag size is determined as 100 bps INFO @ Tue, 30 Jun 2020 02:20:24: #1 tag size = 100 INFO @ Tue, 30 Jun 2020 02:20:24: #1 total tags in treatment: 28099432 INFO @ Tue, 30 Jun 2020 02:20:24: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 02:20:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 02:20:25: #1 tags after filtering in treatment: 28099432 INFO @ Tue, 30 Jun 2020 02:20:25: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 02:20:25: #1 finished! INFO @ Tue, 30 Jun 2020 02:20:25: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 02:20:25: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 02:20:27: #2 number of paired peaks: 4 WARNING @ Tue, 30 Jun 2020 02:20:27: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 02:20:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX645136/SRX645136.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX645136/SRX645136.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX645136/SRX645136.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX645136/SRX645136.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。