Job ID = 12265368 SRX = SRX6430380 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 36702135 spots for SRR9669861/SRR9669861.sra Written 36702135 spots for SRR9669861/SRR9669861.sra fastq に変換しました。 bowtie でマッピング中... Your job 12265672 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:49:34 36702135 reads; of these: 36702135 (100.00%) were paired; of these: 10866214 (29.61%) aligned concordantly 0 times 20192684 (55.02%) aligned concordantly exactly 1 time 5643237 (15.38%) aligned concordantly >1 times ---- 10866214 pairs aligned concordantly 0 times; of these: 3979706 (36.62%) aligned discordantly 1 time ---- 6886508 pairs aligned 0 times concordantly or discordantly; of these: 13773016 mates make up the pairs; of these: 11100637 (80.60%) aligned 0 times 1089820 (7.91%) aligned exactly 1 time 1582559 (11.49%) aligned >1 times 84.88% overall alignment rate Time searching: 00:49:34 Overall time: 00:49:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 28 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 2680238 / 29361606 = 0.0913 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:52:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6430380/SRX6430380.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6430380/SRX6430380.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6430380/SRX6430380.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6430380/SRX6430380.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:52:01: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:52:01: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:52:06: 1000000 INFO @ Sat, 03 Apr 2021 07:52:12: 2000000 INFO @ Sat, 03 Apr 2021 07:52:17: 3000000 INFO @ Sat, 03 Apr 2021 07:52:22: 4000000 INFO @ Sat, 03 Apr 2021 07:52:28: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:52:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6430380/SRX6430380.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6430380/SRX6430380.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6430380/SRX6430380.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6430380/SRX6430380.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:52:31: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:52:31: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:52:33: 6000000 INFO @ Sat, 03 Apr 2021 07:52:36: 1000000 INFO @ Sat, 03 Apr 2021 07:52:39: 7000000 INFO @ Sat, 03 Apr 2021 07:52:42: 2000000 INFO @ Sat, 03 Apr 2021 07:52:44: 8000000 INFO @ Sat, 03 Apr 2021 07:52:48: 3000000 INFO @ Sat, 03 Apr 2021 07:52:51: 9000000 INFO @ Sat, 03 Apr 2021 07:52:53: 4000000 INFO @ Sat, 03 Apr 2021 07:52:56: 10000000 BedGraph に変換中... INFO @ Sat, 03 Apr 2021 07:52:59: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:53:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6430380/SRX6430380.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6430380/SRX6430380.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6430380/SRX6430380.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6430380/SRX6430380.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:53:01: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:53:01: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:53:02: 11000000 INFO @ Sat, 03 Apr 2021 07:53:04: 6000000 INFO @ Sat, 03 Apr 2021 07:53:06: 1000000 INFO @ Sat, 03 Apr 2021 07:53:08: 12000000 INFO @ Sat, 03 Apr 2021 07:53:10: 7000000 INFO @ Sat, 03 Apr 2021 07:53:11: 2000000 INFO @ Sat, 03 Apr 2021 07:53:13: 13000000 INFO @ Sat, 03 Apr 2021 07:53:15: 8000000 INFO @ Sat, 03 Apr 2021 07:53:16: 3000000 INFO @ Sat, 03 Apr 2021 07:53:19: 14000000 INFO @ Sat, 03 Apr 2021 07:53:21: 9000000 INFO @ Sat, 03 Apr 2021 07:53:21: 4000000 INFO @ Sat, 03 Apr 2021 07:53:25: 15000000 INFO @ Sat, 03 Apr 2021 07:53:26: 5000000 INFO @ Sat, 03 Apr 2021 07:53:26: 10000000 INFO @ Sat, 03 Apr 2021 07:53:30: 16000000 INFO @ Sat, 03 Apr 2021 07:53:31: 6000000 INFO @ Sat, 03 Apr 2021 07:53:32: 11000000 INFO @ Sat, 03 Apr 2021 07:53:36: 17000000 INFO @ Sat, 03 Apr 2021 07:53:37: 7000000 INFO @ Sat, 03 Apr 2021 07:53:37: 12000000 INFO @ Sat, 03 Apr 2021 07:53:42: 8000000 INFO @ Sat, 03 Apr 2021 07:53:42: 18000000 INFO @ Sat, 03 Apr 2021 07:53:43: 13000000 INFO @ Sat, 03 Apr 2021 07:53:47: 9000000 INFO @ Sat, 03 Apr 2021 07:53:48: 19000000 INFO @ Sat, 03 Apr 2021 07:53:48: 14000000 INFO @ Sat, 03 Apr 2021 07:53:52: 10000000 INFO @ Sat, 03 Apr 2021 07:53:54: 15000000 INFO @ Sat, 03 Apr 2021 07:53:54: 20000000 INFO @ Sat, 03 Apr 2021 07:53:57: 11000000 INFO @ Sat, 03 Apr 2021 07:53:59: 16000000 INFO @ Sat, 03 Apr 2021 07:54:00: 21000000 INFO @ Sat, 03 Apr 2021 07:54:02: 12000000 INFO @ Sat, 03 Apr 2021 07:54:05: 17000000 INFO @ Sat, 03 Apr 2021 07:54:06: 22000000 INFO @ Sat, 03 Apr 2021 07:54:07: 13000000 INFO @ Sat, 03 Apr 2021 07:54:10: 18000000 INFO @ Sat, 03 Apr 2021 07:54:12: 23000000 INFO @ Sat, 03 Apr 2021 07:54:13: 14000000 INFO @ Sat, 03 Apr 2021 07:54:15: 19000000 INFO @ Sat, 03 Apr 2021 07:54:17: 24000000 INFO @ Sat, 03 Apr 2021 07:54:18: 15000000 INFO @ Sat, 03 Apr 2021 07:54:21: 20000000 INFO @ Sat, 03 Apr 2021 07:54:23: 16000000 INFO @ Sat, 03 Apr 2021 07:54:23: 25000000 INFO @ Sat, 03 Apr 2021 07:54:26: 21000000 INFO @ Sat, 03 Apr 2021 07:54:28: 17000000 INFO @ Sat, 03 Apr 2021 07:54:29: 26000000 INFO @ Sat, 03 Apr 2021 07:54:32: 22000000 INFO @ Sat, 03 Apr 2021 07:54:33: 18000000 INFO @ Sat, 03 Apr 2021 07:54:35: 27000000 INFO @ Sat, 03 Apr 2021 07:54:37: 23000000 INFO @ Sat, 03 Apr 2021 07:54:38: 19000000 INFO @ Sat, 03 Apr 2021 07:54:41: 28000000 INFO @ Sat, 03 Apr 2021 07:54:43: 24000000 INFO @ Sat, 03 Apr 2021 07:54:43: 20000000 INFO @ Sat, 03 Apr 2021 07:54:47: 29000000 INFO @ Sat, 03 Apr 2021 07:54:48: 25000000 INFO @ Sat, 03 Apr 2021 07:54:49: 21000000 INFO @ Sat, 03 Apr 2021 07:54:53: 30000000 INFO @ Sat, 03 Apr 2021 07:54:54: 26000000 INFO @ Sat, 03 Apr 2021 07:54:54: 22000000 INFO @ Sat, 03 Apr 2021 07:54:58: 31000000 INFO @ Sat, 03 Apr 2021 07:54:59: 23000000 INFO @ Sat, 03 Apr 2021 07:54:59: 27000000 INFO @ Sat, 03 Apr 2021 07:55:04: 24000000 INFO @ Sat, 03 Apr 2021 07:55:04: 32000000 INFO @ Sat, 03 Apr 2021 07:55:05: 28000000 INFO @ Sat, 03 Apr 2021 07:55:09: 25000000 INFO @ Sat, 03 Apr 2021 07:55:10: 29000000 INFO @ Sat, 03 Apr 2021 07:55:10: 33000000 INFO @ Sat, 03 Apr 2021 07:55:15: 26000000 INFO @ Sat, 03 Apr 2021 07:55:16: 30000000 INFO @ Sat, 03 Apr 2021 07:55:16: 34000000 INFO @ Sat, 03 Apr 2021 07:55:20: 27000000 INFO @ Sat, 03 Apr 2021 07:55:21: 31000000 INFO @ Sat, 03 Apr 2021 07:55:22: 35000000 INFO @ Sat, 03 Apr 2021 07:55:25: 28000000 INFO @ Sat, 03 Apr 2021 07:55:27: 32000000 INFO @ Sat, 03 Apr 2021 07:55:28: 36000000 INFO @ Sat, 03 Apr 2021 07:55:31: 29000000 INFO @ Sat, 03 Apr 2021 07:55:32: 33000000 INFO @ Sat, 03 Apr 2021 07:55:34: 37000000 INFO @ Sat, 03 Apr 2021 07:55:36: 30000000 INFO @ Sat, 03 Apr 2021 07:55:38: 34000000 INFO @ Sat, 03 Apr 2021 07:55:40: 38000000 INFO @ Sat, 03 Apr 2021 07:55:41: 31000000 INFO @ Sat, 03 Apr 2021 07:55:44: 35000000 INFO @ Sat, 03 Apr 2021 07:55:46: 39000000 INFO @ Sat, 03 Apr 2021 07:55:46: 32000000 INFO @ Sat, 03 Apr 2021 07:55:50: 36000000 INFO @ Sat, 03 Apr 2021 07:55:52: 33000000 INFO @ Sat, 03 Apr 2021 07:55:52: 40000000 INFO @ Sat, 03 Apr 2021 07:55:55: 37000000 INFO @ Sat, 03 Apr 2021 07:55:57: 34000000 INFO @ Sat, 03 Apr 2021 07:55:58: 41000000 INFO @ Sat, 03 Apr 2021 07:56:01: 38000000 INFO @ Sat, 03 Apr 2021 07:56:02: 35000000 INFO @ Sat, 03 Apr 2021 07:56:04: 42000000 INFO @ Sat, 03 Apr 2021 07:56:07: 36000000 INFO @ Sat, 03 Apr 2021 07:56:07: 39000000 INFO @ Sat, 03 Apr 2021 07:56:10: 43000000 INFO @ Sat, 03 Apr 2021 07:56:12: 37000000 INFO @ Sat, 03 Apr 2021 07:56:13: 40000000 INFO @ Sat, 03 Apr 2021 07:56:16: 44000000 INFO @ Sat, 03 Apr 2021 07:56:17: 38000000 INFO @ Sat, 03 Apr 2021 07:56:19: 41000000 INFO @ Sat, 03 Apr 2021 07:56:22: 45000000 INFO @ Sat, 03 Apr 2021 07:56:22: 39000000 INFO @ Sat, 03 Apr 2021 07:56:24: 42000000 INFO @ Sat, 03 Apr 2021 07:56:27: 40000000 INFO @ Sat, 03 Apr 2021 07:56:28: 46000000 INFO @ Sat, 03 Apr 2021 07:56:30: 43000000 INFO @ Sat, 03 Apr 2021 07:56:32: 41000000 INFO @ Sat, 03 Apr 2021 07:56:34: 47000000 INFO @ Sat, 03 Apr 2021 07:56:36: 44000000 INFO @ Sat, 03 Apr 2021 07:56:37: 42000000 INFO @ Sat, 03 Apr 2021 07:56:40: 48000000 INFO @ Sat, 03 Apr 2021 07:56:42: 45000000 INFO @ Sat, 03 Apr 2021 07:56:42: 43000000 INFO @ Sat, 03 Apr 2021 07:56:46: 49000000 INFO @ Sat, 03 Apr 2021 07:56:47: 44000000 INFO @ Sat, 03 Apr 2021 07:56:47: 46000000 INFO @ Sat, 03 Apr 2021 07:56:51: 50000000 INFO @ Sat, 03 Apr 2021 07:56:52: 45000000 INFO @ Sat, 03 Apr 2021 07:56:53: 47000000 INFO @ Sat, 03 Apr 2021 07:56:57: 51000000 INFO @ Sat, 03 Apr 2021 07:56:58: 46000000 INFO @ Sat, 03 Apr 2021 07:56:59: 48000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 07:57:03: 47000000 INFO @ Sat, 03 Apr 2021 07:57:03: 52000000 INFO @ Sat, 03 Apr 2021 07:57:05: 49000000 INFO @ Sat, 03 Apr 2021 07:57:08: 48000000 INFO @ Sat, 03 Apr 2021 07:57:08: 53000000 INFO @ Sat, 03 Apr 2021 07:57:10: 50000000 INFO @ Sat, 03 Apr 2021 07:57:13: 49000000 INFO @ Sat, 03 Apr 2021 07:57:14: 54000000 INFO @ Sat, 03 Apr 2021 07:57:16: 51000000 INFO @ Sat, 03 Apr 2021 07:57:18: 50000000 INFO @ Sat, 03 Apr 2021 07:57:20: 55000000 INFO @ Sat, 03 Apr 2021 07:57:22: 52000000 INFO @ Sat, 03 Apr 2021 07:57:23: 51000000 INFO @ Sat, 03 Apr 2021 07:57:26: 56000000 INFO @ Sat, 03 Apr 2021 07:57:28: 53000000 INFO @ Sat, 03 Apr 2021 07:57:28: 52000000 INFO @ Sat, 03 Apr 2021 07:57:32: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:57:32: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:57:32: #1 total tags in treatment: 23559041 INFO @ Sat, 03 Apr 2021 07:57:32: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:57:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:57:32: #1 tags after filtering in treatment: 18721559 INFO @ Sat, 03 Apr 2021 07:57:32: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 03 Apr 2021 07:57:32: #1 finished! INFO @ Sat, 03 Apr 2021 07:57:32: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:57:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:57:33: 53000000 INFO @ Sat, 03 Apr 2021 07:57:33: #2 number of paired peaks: 52 WARNING @ Sat, 03 Apr 2021 07:57:33: Too few paired peaks (52) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 07:57:33: Process for pairing-model is terminated! INFO @ Sat, 03 Apr 2021 07:57:33: 54000000 cut: /home/okishinya/chipatlas/results/dm3/SRX6430380/SRX6430380.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430380/SRX6430380.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430380/SRX6430380.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430380/SRX6430380.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:57:38: 54000000 INFO @ Sat, 03 Apr 2021 07:57:39: 55000000 INFO @ Sat, 03 Apr 2021 07:57:43: 55000000 INFO @ Sat, 03 Apr 2021 07:57:45: 56000000 INFO @ Sat, 03 Apr 2021 07:57:48: 56000000 INFO @ Sat, 03 Apr 2021 07:57:50: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:57:50: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:57:50: #1 total tags in treatment: 23559041 INFO @ Sat, 03 Apr 2021 07:57:50: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:57:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:57:51: #1 tags after filtering in treatment: 18721559 INFO @ Sat, 03 Apr 2021 07:57:51: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 03 Apr 2021 07:57:51: #1 finished! INFO @ Sat, 03 Apr 2021 07:57:51: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:57:51: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:57:52: #2 number of paired peaks: 52 WARNING @ Sat, 03 Apr 2021 07:57:52: Too few paired peaks (52) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 07:57:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6430380/SRX6430380.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430380/SRX6430380.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430380/SRX6430380.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430380/SRX6430380.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:57:52: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:57:52: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:57:52: #1 total tags in treatment: 23559041 INFO @ Sat, 03 Apr 2021 07:57:52: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:57:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:57:53: #1 tags after filtering in treatment: 18721559 INFO @ Sat, 03 Apr 2021 07:57:53: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 03 Apr 2021 07:57:53: #1 finished! INFO @ Sat, 03 Apr 2021 07:57:53: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:57:53: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:57:54: #2 number of paired peaks: 52 WARNING @ Sat, 03 Apr 2021 07:57:54: Too few paired peaks (52) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 07:57:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6430380/SRX6430380.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430380/SRX6430380.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430380/SRX6430380.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430380/SRX6430380.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。