Job ID = 12265356 SRX = SRX6430372 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 46277487 spots for SRR9669869/SRR9669869.sra Written 46277487 spots for SRR9669869/SRR9669869.sra fastq に変換しました。 bowtie でマッピング中... Your job 12265588 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:30:25 46277487 reads; of these: 46277487 (100.00%) were paired; of these: 26580495 (57.44%) aligned concordantly 0 times 12020194 (25.97%) aligned concordantly exactly 1 time 7676798 (16.59%) aligned concordantly >1 times ---- 26580495 pairs aligned concordantly 0 times; of these: 2035305 (7.66%) aligned discordantly 1 time ---- 24545190 pairs aligned 0 times concordantly or discordantly; of these: 49090380 mates make up the pairs; of these: 47100281 (95.95%) aligned 0 times 684534 (1.39%) aligned exactly 1 time 1305565 (2.66%) aligned >1 times 49.11% overall alignment rate Time searching: 00:30:25 Overall time: 00:30:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 4511836 / 21458064 = 0.2103 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:25:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6430372/SRX6430372.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6430372/SRX6430372.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6430372/SRX6430372.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6430372/SRX6430372.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:25:00: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:25:00: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:25:05: 1000000 INFO @ Sat, 03 Apr 2021 07:25:10: 2000000 INFO @ Sat, 03 Apr 2021 07:25:15: 3000000 INFO @ Sat, 03 Apr 2021 07:25:20: 4000000 INFO @ Sat, 03 Apr 2021 07:25:25: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:25:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6430372/SRX6430372.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6430372/SRX6430372.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6430372/SRX6430372.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6430372/SRX6430372.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:25:30: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:25:30: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:25:30: 6000000 INFO @ Sat, 03 Apr 2021 07:25:35: 7000000 INFO @ Sat, 03 Apr 2021 07:25:36: 1000000 INFO @ Sat, 03 Apr 2021 07:25:41: 8000000 INFO @ Sat, 03 Apr 2021 07:25:43: 2000000 INFO @ Sat, 03 Apr 2021 07:25:47: 9000000 INFO @ Sat, 03 Apr 2021 07:25:49: 3000000 INFO @ Sat, 03 Apr 2021 07:25:53: 10000000 INFO @ Sat, 03 Apr 2021 07:25:56: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:25:59: 11000000 INFO @ Sat, 03 Apr 2021 07:26:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6430372/SRX6430372.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6430372/SRX6430372.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6430372/SRX6430372.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6430372/SRX6430372.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:26:00: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:26:00: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:26:02: 5000000 INFO @ Sat, 03 Apr 2021 07:26:05: 12000000 INFO @ Sat, 03 Apr 2021 07:26:06: 1000000 INFO @ Sat, 03 Apr 2021 07:26:09: 6000000 INFO @ Sat, 03 Apr 2021 07:26:10: 13000000 INFO @ Sat, 03 Apr 2021 07:26:13: 2000000 INFO @ Sat, 03 Apr 2021 07:26:15: 7000000 INFO @ Sat, 03 Apr 2021 07:26:16: 14000000 INFO @ Sat, 03 Apr 2021 07:26:20: 3000000 INFO @ Sat, 03 Apr 2021 07:26:22: 8000000 INFO @ Sat, 03 Apr 2021 07:26:22: 15000000 INFO @ Sat, 03 Apr 2021 07:26:27: 4000000 INFO @ Sat, 03 Apr 2021 07:26:28: 16000000 INFO @ Sat, 03 Apr 2021 07:26:28: 9000000 INFO @ Sat, 03 Apr 2021 07:26:34: 17000000 INFO @ Sat, 03 Apr 2021 07:26:34: 5000000 INFO @ Sat, 03 Apr 2021 07:26:35: 10000000 INFO @ Sat, 03 Apr 2021 07:26:40: 18000000 INFO @ Sat, 03 Apr 2021 07:26:41: 6000000 INFO @ Sat, 03 Apr 2021 07:26:41: 11000000 INFO @ Sat, 03 Apr 2021 07:26:45: 19000000 INFO @ Sat, 03 Apr 2021 07:26:48: 7000000 INFO @ Sat, 03 Apr 2021 07:26:48: 12000000 INFO @ Sat, 03 Apr 2021 07:26:51: 20000000 INFO @ Sat, 03 Apr 2021 07:26:54: 8000000 INFO @ Sat, 03 Apr 2021 07:26:55: 13000000 INFO @ Sat, 03 Apr 2021 07:26:57: 21000000 INFO @ Sat, 03 Apr 2021 07:27:01: 9000000 INFO @ Sat, 03 Apr 2021 07:27:01: 14000000 INFO @ Sat, 03 Apr 2021 07:27:03: 22000000 INFO @ Sat, 03 Apr 2021 07:27:08: 10000000 INFO @ Sat, 03 Apr 2021 07:27:08: 15000000 INFO @ Sat, 03 Apr 2021 07:27:09: 23000000 INFO @ Sat, 03 Apr 2021 07:27:14: 16000000 INFO @ Sat, 03 Apr 2021 07:27:14: 11000000 INFO @ Sat, 03 Apr 2021 07:27:15: 24000000 INFO @ Sat, 03 Apr 2021 07:27:21: 17000000 INFO @ Sat, 03 Apr 2021 07:27:21: 12000000 INFO @ Sat, 03 Apr 2021 07:27:21: 25000000 INFO @ Sat, 03 Apr 2021 07:27:27: 26000000 INFO @ Sat, 03 Apr 2021 07:27:27: 18000000 INFO @ Sat, 03 Apr 2021 07:27:28: 13000000 INFO @ Sat, 03 Apr 2021 07:27:33: 27000000 INFO @ Sat, 03 Apr 2021 07:27:34: 19000000 INFO @ Sat, 03 Apr 2021 07:27:35: 14000000 INFO @ Sat, 03 Apr 2021 07:27:39: 28000000 INFO @ Sat, 03 Apr 2021 07:27:40: 20000000 INFO @ Sat, 03 Apr 2021 07:27:42: 15000000 INFO @ Sat, 03 Apr 2021 07:27:44: 29000000 INFO @ Sat, 03 Apr 2021 07:27:47: 21000000 INFO @ Sat, 03 Apr 2021 07:27:48: 16000000 INFO @ Sat, 03 Apr 2021 07:27:50: 30000000 INFO @ Sat, 03 Apr 2021 07:27:53: 22000000 INFO @ Sat, 03 Apr 2021 07:27:55: 17000000 INFO @ Sat, 03 Apr 2021 07:27:57: 31000000 INFO @ Sat, 03 Apr 2021 07:28:00: 23000000 INFO @ Sat, 03 Apr 2021 07:28:02: 18000000 INFO @ Sat, 03 Apr 2021 07:28:02: 32000000 INFO @ Sat, 03 Apr 2021 07:28:06: 24000000 INFO @ Sat, 03 Apr 2021 07:28:08: 33000000 INFO @ Sat, 03 Apr 2021 07:28:08: 19000000 INFO @ Sat, 03 Apr 2021 07:28:12: 25000000 INFO @ Sat, 03 Apr 2021 07:28:14: 34000000 INFO @ Sat, 03 Apr 2021 07:28:15: 20000000 INFO @ Sat, 03 Apr 2021 07:28:19: 26000000 INFO @ Sat, 03 Apr 2021 07:28:20: 35000000 INFO @ Sat, 03 Apr 2021 07:28:21: 21000000 INFO @ Sat, 03 Apr 2021 07:28:25: 27000000 INFO @ Sat, 03 Apr 2021 07:28:26: 36000000 INFO @ Sat, 03 Apr 2021 07:28:27: 22000000 INFO @ Sat, 03 Apr 2021 07:28:29: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:28:29: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:28:29: #1 total tags in treatment: 15276445 INFO @ Sat, 03 Apr 2021 07:28:29: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:28:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:28:29: #1 tags after filtering in treatment: 12392612 INFO @ Sat, 03 Apr 2021 07:28:29: #1 Redundant rate of treatment: 0.19 INFO @ Sat, 03 Apr 2021 07:28:29: #1 finished! INFO @ Sat, 03 Apr 2021 07:28:29: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:28:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:28:30: #2 number of paired peaks: 88 WARNING @ Sat, 03 Apr 2021 07:28:30: Too few paired peaks (88) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 07:28:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6430372/SRX6430372.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430372/SRX6430372.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430372/SRX6430372.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430372/SRX6430372.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:28:31: 28000000 INFO @ Sat, 03 Apr 2021 07:28:34: 23000000 INFO @ Sat, 03 Apr 2021 07:28:38: 29000000 INFO @ Sat, 03 Apr 2021 07:28:40: 24000000 INFO @ Sat, 03 Apr 2021 07:28:44: 30000000 INFO @ Sat, 03 Apr 2021 07:28:46: 25000000 INFO @ Sat, 03 Apr 2021 07:28:51: 31000000 INFO @ Sat, 03 Apr 2021 07:28:53: 26000000 INFO @ Sat, 03 Apr 2021 07:28:58: 32000000 INFO @ Sat, 03 Apr 2021 07:29:00: 27000000 INFO @ Sat, 03 Apr 2021 07:29:04: 33000000 INFO @ Sat, 03 Apr 2021 07:29:06: 28000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 07:29:11: 34000000 INFO @ Sat, 03 Apr 2021 07:29:13: 29000000 INFO @ Sat, 03 Apr 2021 07:29:17: 35000000 INFO @ Sat, 03 Apr 2021 07:29:19: 30000000 INFO @ Sat, 03 Apr 2021 07:29:24: 36000000 INFO @ Sat, 03 Apr 2021 07:29:26: 31000000 INFO @ Sat, 03 Apr 2021 07:29:27: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:29:27: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:29:27: #1 total tags in treatment: 15276445 INFO @ Sat, 03 Apr 2021 07:29:27: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:29:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:29:27: #1 tags after filtering in treatment: 12392612 INFO @ Sat, 03 Apr 2021 07:29:27: #1 Redundant rate of treatment: 0.19 INFO @ Sat, 03 Apr 2021 07:29:27: #1 finished! INFO @ Sat, 03 Apr 2021 07:29:27: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:29:27: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:29:28: #2 number of paired peaks: 88 WARNING @ Sat, 03 Apr 2021 07:29:28: Too few paired peaks (88) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 07:29:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6430372/SRX6430372.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430372/SRX6430372.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430372/SRX6430372.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430372/SRX6430372.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:29:32: 32000000 INFO @ Sat, 03 Apr 2021 07:29:39: 33000000 INFO @ Sat, 03 Apr 2021 07:29:45: 34000000 INFO @ Sat, 03 Apr 2021 07:29:51: 35000000 INFO @ Sat, 03 Apr 2021 07:29:57: 36000000 INFO @ Sat, 03 Apr 2021 07:30:00: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:30:00: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:30:00: #1 total tags in treatment: 15276445 INFO @ Sat, 03 Apr 2021 07:30:00: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:30:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:30:00: #1 tags after filtering in treatment: 12392612 INFO @ Sat, 03 Apr 2021 07:30:00: #1 Redundant rate of treatment: 0.19 INFO @ Sat, 03 Apr 2021 07:30:00: #1 finished! INFO @ Sat, 03 Apr 2021 07:30:00: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:30:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:30:01: #2 number of paired peaks: 88 WARNING @ Sat, 03 Apr 2021 07:30:01: Too few paired peaks (88) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 07:30:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6430372/SRX6430372.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430372/SRX6430372.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430372/SRX6430372.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430372/SRX6430372.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。