Job ID = 12265347 SRX = SRX6430357 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 35174835 spots for SRR9669884/SRR9669884.sra Written 35174835 spots for SRR9669884/SRR9669884.sra fastq に変換しました。 bowtie でマッピング中... Your job 12265646 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:48:48 35174835 reads; of these: 35174835 (100.00%) were paired; of these: 11025382 (31.34%) aligned concordantly 0 times 18278389 (51.96%) aligned concordantly exactly 1 time 5871064 (16.69%) aligned concordantly >1 times ---- 11025382 pairs aligned concordantly 0 times; of these: 4016064 (36.43%) aligned discordantly 1 time ---- 7009318 pairs aligned 0 times concordantly or discordantly; of these: 14018636 mates make up the pairs; of these: 10915271 (77.86%) aligned 0 times 1397277 (9.97%) aligned exactly 1 time 1706088 (12.17%) aligned >1 times 84.48% overall alignment rate Time searching: 00:48:48 Overall time: 00:48:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 28 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 3532341 / 27723244 = 0.1274 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:48:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6430357/SRX6430357.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6430357/SRX6430357.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6430357/SRX6430357.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6430357/SRX6430357.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:48:40: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:48:40: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:48:45: 1000000 INFO @ Sat, 03 Apr 2021 07:48:51: 2000000 INFO @ Sat, 03 Apr 2021 07:48:57: 3000000 INFO @ Sat, 03 Apr 2021 07:49:03: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:49:09: 5000000 INFO @ Sat, 03 Apr 2021 07:49:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6430357/SRX6430357.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6430357/SRX6430357.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6430357/SRX6430357.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6430357/SRX6430357.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:49:10: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:49:10: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:49:16: 6000000 INFO @ Sat, 03 Apr 2021 07:49:16: 1000000 INFO @ Sat, 03 Apr 2021 07:49:22: 7000000 INFO @ Sat, 03 Apr 2021 07:49:23: 2000000 INFO @ Sat, 03 Apr 2021 07:49:29: 8000000 INFO @ Sat, 03 Apr 2021 07:49:29: 3000000 INFO @ Sat, 03 Apr 2021 07:49:35: 9000000 INFO @ Sat, 03 Apr 2021 07:49:36: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:49:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6430357/SRX6430357.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6430357/SRX6430357.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6430357/SRX6430357.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6430357/SRX6430357.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:49:40: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:49:40: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:49:42: 10000000 INFO @ Sat, 03 Apr 2021 07:49:43: 5000000 INFO @ Sat, 03 Apr 2021 07:49:47: 1000000 INFO @ Sat, 03 Apr 2021 07:49:49: 11000000 INFO @ Sat, 03 Apr 2021 07:49:50: 6000000 INFO @ Sat, 03 Apr 2021 07:49:54: 2000000 INFO @ Sat, 03 Apr 2021 07:49:56: 12000000 INFO @ Sat, 03 Apr 2021 07:49:57: 7000000 INFO @ Sat, 03 Apr 2021 07:50:00: 3000000 INFO @ Sat, 03 Apr 2021 07:50:04: 8000000 INFO @ Sat, 03 Apr 2021 07:50:04: 13000000 INFO @ Sat, 03 Apr 2021 07:50:07: 4000000 INFO @ Sat, 03 Apr 2021 07:50:11: 9000000 INFO @ Sat, 03 Apr 2021 07:50:11: 14000000 INFO @ Sat, 03 Apr 2021 07:50:15: 5000000 INFO @ Sat, 03 Apr 2021 07:50:18: 10000000 INFO @ Sat, 03 Apr 2021 07:50:18: 15000000 INFO @ Sat, 03 Apr 2021 07:50:22: 6000000 INFO @ Sat, 03 Apr 2021 07:50:25: 11000000 INFO @ Sat, 03 Apr 2021 07:50:25: 16000000 INFO @ Sat, 03 Apr 2021 07:50:29: 7000000 INFO @ Sat, 03 Apr 2021 07:50:32: 12000000 INFO @ Sat, 03 Apr 2021 07:50:32: 17000000 INFO @ Sat, 03 Apr 2021 07:50:36: 8000000 INFO @ Sat, 03 Apr 2021 07:50:39: 18000000 INFO @ Sat, 03 Apr 2021 07:50:39: 13000000 INFO @ Sat, 03 Apr 2021 07:50:43: 9000000 INFO @ Sat, 03 Apr 2021 07:50:46: 19000000 INFO @ Sat, 03 Apr 2021 07:50:46: 14000000 INFO @ Sat, 03 Apr 2021 07:50:49: 10000000 INFO @ Sat, 03 Apr 2021 07:50:53: 20000000 INFO @ Sat, 03 Apr 2021 07:50:53: 15000000 INFO @ Sat, 03 Apr 2021 07:50:56: 11000000 INFO @ Sat, 03 Apr 2021 07:51:00: 21000000 INFO @ Sat, 03 Apr 2021 07:51:00: 16000000 INFO @ Sat, 03 Apr 2021 07:51:03: 12000000 INFO @ Sat, 03 Apr 2021 07:51:07: 17000000 INFO @ Sat, 03 Apr 2021 07:51:07: 22000000 INFO @ Sat, 03 Apr 2021 07:51:10: 13000000 INFO @ Sat, 03 Apr 2021 07:51:14: 18000000 INFO @ Sat, 03 Apr 2021 07:51:14: 23000000 INFO @ Sat, 03 Apr 2021 07:51:17: 14000000 INFO @ Sat, 03 Apr 2021 07:51:21: 19000000 INFO @ Sat, 03 Apr 2021 07:51:21: 24000000 INFO @ Sat, 03 Apr 2021 07:51:24: 15000000 INFO @ Sat, 03 Apr 2021 07:51:28: 20000000 INFO @ Sat, 03 Apr 2021 07:51:28: 25000000 INFO @ Sat, 03 Apr 2021 07:51:31: 16000000 INFO @ Sat, 03 Apr 2021 07:51:35: 21000000 INFO @ Sat, 03 Apr 2021 07:51:35: 26000000 INFO @ Sat, 03 Apr 2021 07:51:37: 17000000 INFO @ Sat, 03 Apr 2021 07:51:42: 22000000 INFO @ Sat, 03 Apr 2021 07:51:42: 27000000 INFO @ Sat, 03 Apr 2021 07:51:44: 18000000 INFO @ Sat, 03 Apr 2021 07:51:49: 23000000 INFO @ Sat, 03 Apr 2021 07:51:49: 28000000 INFO @ Sat, 03 Apr 2021 07:51:51: 19000000 INFO @ Sat, 03 Apr 2021 07:51:56: 24000000 INFO @ Sat, 03 Apr 2021 07:51:56: 29000000 INFO @ Sat, 03 Apr 2021 07:51:58: 20000000 INFO @ Sat, 03 Apr 2021 07:52:03: 25000000 INFO @ Sat, 03 Apr 2021 07:52:03: 30000000 INFO @ Sat, 03 Apr 2021 07:52:05: 21000000 INFO @ Sat, 03 Apr 2021 07:52:09: 26000000 INFO @ Sat, 03 Apr 2021 07:52:10: 31000000 INFO @ Sat, 03 Apr 2021 07:52:12: 22000000 INFO @ Sat, 03 Apr 2021 07:52:16: 27000000 INFO @ Sat, 03 Apr 2021 07:52:17: 32000000 INFO @ Sat, 03 Apr 2021 07:52:18: 23000000 INFO @ Sat, 03 Apr 2021 07:52:23: 28000000 INFO @ Sat, 03 Apr 2021 07:52:24: 33000000 INFO @ Sat, 03 Apr 2021 07:52:25: 24000000 INFO @ Sat, 03 Apr 2021 07:52:30: 29000000 INFO @ Sat, 03 Apr 2021 07:52:31: 34000000 INFO @ Sat, 03 Apr 2021 07:52:32: 25000000 INFO @ Sat, 03 Apr 2021 07:52:37: 30000000 INFO @ Sat, 03 Apr 2021 07:52:38: 35000000 INFO @ Sat, 03 Apr 2021 07:52:39: 26000000 INFO @ Sat, 03 Apr 2021 07:52:44: 31000000 INFO @ Sat, 03 Apr 2021 07:52:45: 36000000 INFO @ Sat, 03 Apr 2021 07:52:45: 27000000 INFO @ Sat, 03 Apr 2021 07:52:51: 32000000 INFO @ Sat, 03 Apr 2021 07:52:51: 37000000 INFO @ Sat, 03 Apr 2021 07:52:52: 28000000 INFO @ Sat, 03 Apr 2021 07:52:58: 33000000 INFO @ Sat, 03 Apr 2021 07:52:58: 38000000 INFO @ Sat, 03 Apr 2021 07:52:59: 29000000 INFO @ Sat, 03 Apr 2021 07:53:05: 34000000 INFO @ Sat, 03 Apr 2021 07:53:05: 39000000 INFO @ Sat, 03 Apr 2021 07:53:06: 30000000 INFO @ Sat, 03 Apr 2021 07:53:11: 40000000 INFO @ Sat, 03 Apr 2021 07:53:11: 35000000 INFO @ Sat, 03 Apr 2021 07:53:13: 31000000 INFO @ Sat, 03 Apr 2021 07:53:18: 41000000 INFO @ Sat, 03 Apr 2021 07:53:18: 36000000 INFO @ Sat, 03 Apr 2021 07:53:20: 32000000 INFO @ Sat, 03 Apr 2021 07:53:25: 42000000 INFO @ Sat, 03 Apr 2021 07:53:26: 37000000 INFO @ Sat, 03 Apr 2021 07:53:27: 33000000 INFO @ Sat, 03 Apr 2021 07:53:32: 43000000 INFO @ Sat, 03 Apr 2021 07:53:32: 38000000 INFO @ Sat, 03 Apr 2021 07:53:34: 34000000 INFO @ Sat, 03 Apr 2021 07:53:39: 44000000 INFO @ Sat, 03 Apr 2021 07:53:39: 39000000 INFO @ Sat, 03 Apr 2021 07:53:40: 35000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 07:53:45: 45000000 INFO @ Sat, 03 Apr 2021 07:53:46: 40000000 INFO @ Sat, 03 Apr 2021 07:53:47: 36000000 INFO @ Sat, 03 Apr 2021 07:53:52: 46000000 INFO @ Sat, 03 Apr 2021 07:53:52: 41000000 INFO @ Sat, 03 Apr 2021 07:53:54: 37000000 INFO @ Sat, 03 Apr 2021 07:53:58: 47000000 INFO @ Sat, 03 Apr 2021 07:53:59: 42000000 INFO @ Sat, 03 Apr 2021 07:54:01: 38000000 INFO @ Sat, 03 Apr 2021 07:54:05: 48000000 INFO @ Sat, 03 Apr 2021 07:54:06: 43000000 INFO @ Sat, 03 Apr 2021 07:54:07: 39000000 INFO @ Sat, 03 Apr 2021 07:54:11: 49000000 INFO @ Sat, 03 Apr 2021 07:54:13: 44000000 INFO @ Sat, 03 Apr 2021 07:54:14: 40000000 INFO @ Sat, 03 Apr 2021 07:54:18: 50000000 INFO @ Sat, 03 Apr 2021 07:54:20: 45000000 INFO @ Sat, 03 Apr 2021 07:54:20: 41000000 INFO @ Sat, 03 Apr 2021 07:54:24: 51000000 INFO @ Sat, 03 Apr 2021 07:54:27: 46000000 INFO @ Sat, 03 Apr 2021 07:54:27: 42000000 INFO @ Sat, 03 Apr 2021 07:54:31: 52000000 INFO @ Sat, 03 Apr 2021 07:54:33: 47000000 INFO @ Sat, 03 Apr 2021 07:54:34: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:54:34: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:54:34: #1 total tags in treatment: 20963719 INFO @ Sat, 03 Apr 2021 07:54:34: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:54:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:54:34: 43000000 INFO @ Sat, 03 Apr 2021 07:54:34: #1 tags after filtering in treatment: 16915137 INFO @ Sat, 03 Apr 2021 07:54:34: #1 Redundant rate of treatment: 0.19 INFO @ Sat, 03 Apr 2021 07:54:34: #1 finished! INFO @ Sat, 03 Apr 2021 07:54:34: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:54:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:54:35: #2 number of paired peaks: 86 WARNING @ Sat, 03 Apr 2021 07:54:35: Too few paired peaks (86) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 07:54:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6430357/SRX6430357.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430357/SRX6430357.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430357/SRX6430357.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430357/SRX6430357.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:54:40: 48000000 INFO @ Sat, 03 Apr 2021 07:54:40: 44000000 INFO @ Sat, 03 Apr 2021 07:54:46: 49000000 INFO @ Sat, 03 Apr 2021 07:54:46: 45000000 INFO @ Sat, 03 Apr 2021 07:54:52: 50000000 INFO @ Sat, 03 Apr 2021 07:54:52: 46000000 INFO @ Sat, 03 Apr 2021 07:54:58: 51000000 INFO @ Sat, 03 Apr 2021 07:54:58: 47000000 INFO @ Sat, 03 Apr 2021 07:55:05: 52000000 INFO @ Sat, 03 Apr 2021 07:55:05: 48000000 INFO @ Sat, 03 Apr 2021 07:55:07: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:55:07: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:55:07: #1 total tags in treatment: 20963719 INFO @ Sat, 03 Apr 2021 07:55:07: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:55:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:55:08: #1 tags after filtering in treatment: 16915137 INFO @ Sat, 03 Apr 2021 07:55:08: #1 Redundant rate of treatment: 0.19 INFO @ Sat, 03 Apr 2021 07:55:08: #1 finished! INFO @ Sat, 03 Apr 2021 07:55:08: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:55:08: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:55:09: #2 number of paired peaks: 86 WARNING @ Sat, 03 Apr 2021 07:55:09: Too few paired peaks (86) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 07:55:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6430357/SRX6430357.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430357/SRX6430357.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430357/SRX6430357.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430357/SRX6430357.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:55:11: 49000000 INFO @ Sat, 03 Apr 2021 07:55:16: 50000000 INFO @ Sat, 03 Apr 2021 07:55:22: 51000000 INFO @ Sat, 03 Apr 2021 07:55:27: 52000000 INFO @ Sat, 03 Apr 2021 07:55:29: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:55:29: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:55:29: #1 total tags in treatment: 20963719 INFO @ Sat, 03 Apr 2021 07:55:29: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:55:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:55:30: #1 tags after filtering in treatment: 16915137 INFO @ Sat, 03 Apr 2021 07:55:30: #1 Redundant rate of treatment: 0.19 INFO @ Sat, 03 Apr 2021 07:55:30: #1 finished! INFO @ Sat, 03 Apr 2021 07:55:30: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:55:30: #2 looking for paired plus/minus strand peaks... BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 07:55:31: #2 number of paired peaks: 86 WARNING @ Sat, 03 Apr 2021 07:55:31: Too few paired peaks (86) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 07:55:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6430357/SRX6430357.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430357/SRX6430357.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430357/SRX6430357.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430357/SRX6430357.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling