Job ID = 5721055 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-04-15T19:06:57 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T19:06:57 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T19:19:51 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T19:19:51 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T19:19:51 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T19:19:51 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T19:47:07 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T19:55:23 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T20:36:20 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T20:36:29 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T20:39:03 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T20:45:22 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T20:49:58 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 63,243,595 reads read : 126,487,190 reads written : 126,487,190 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 03:00:24 63243595 reads; of these: 63243595 (100.00%) were paired; of these: 13184443 (20.85%) aligned concordantly 0 times 39896434 (63.08%) aligned concordantly exactly 1 time 10162718 (16.07%) aligned concordantly >1 times ---- 13184443 pairs aligned concordantly 0 times; of these: 3753349 (28.47%) aligned discordantly 1 time ---- 9431094 pairs aligned 0 times concordantly or discordantly; of these: 18862188 mates make up the pairs; of these: 14729900 (78.09%) aligned 0 times 1697740 (9.00%) aligned exactly 1 time 2434548 (12.91%) aligned >1 times 88.35% overall alignment rate Time searching: 03:00:24 Overall time: 03:00:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 84 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 38429622 / 53558332 = 0.7175 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 09:36:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6386786/SRX6386786.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6386786/SRX6386786.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6386786/SRX6386786.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6386786/SRX6386786.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 09:36:41: #1 read tag files... INFO @ Thu, 16 Apr 2020 09:36:41: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 09:36:49: 1000000 INFO @ Thu, 16 Apr 2020 09:36:57: 2000000 INFO @ Thu, 16 Apr 2020 09:37:05: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 09:37:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6386786/SRX6386786.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6386786/SRX6386786.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6386786/SRX6386786.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6386786/SRX6386786.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 09:37:11: #1 read tag files... INFO @ Thu, 16 Apr 2020 09:37:11: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 09:37:13: 4000000 INFO @ Thu, 16 Apr 2020 09:37:20: 1000000 INFO @ Thu, 16 Apr 2020 09:37:22: 5000000 INFO @ Thu, 16 Apr 2020 09:37:28: 2000000 INFO @ Thu, 16 Apr 2020 09:37:30: 6000000 INFO @ Thu, 16 Apr 2020 09:37:37: 3000000 INFO @ Thu, 16 Apr 2020 09:37:39: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 09:37:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6386786/SRX6386786.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6386786/SRX6386786.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6386786/SRX6386786.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6386786/SRX6386786.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 09:37:41: #1 read tag files... INFO @ Thu, 16 Apr 2020 09:37:41: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 09:37:46: 4000000 INFO @ Thu, 16 Apr 2020 09:37:47: 8000000 INFO @ Thu, 16 Apr 2020 09:37:50: 1000000 INFO @ Thu, 16 Apr 2020 09:37:55: 5000000 INFO @ Thu, 16 Apr 2020 09:37:56: 9000000 INFO @ Thu, 16 Apr 2020 09:37:58: 2000000 INFO @ Thu, 16 Apr 2020 09:38:03: 6000000 INFO @ Thu, 16 Apr 2020 09:38:04: 10000000 INFO @ Thu, 16 Apr 2020 09:38:07: 3000000 INFO @ Thu, 16 Apr 2020 09:38:12: 7000000 INFO @ Thu, 16 Apr 2020 09:38:13: 11000000 INFO @ Thu, 16 Apr 2020 09:38:15: 4000000 INFO @ Thu, 16 Apr 2020 09:38:21: 8000000 INFO @ Thu, 16 Apr 2020 09:38:22: 12000000 INFO @ Thu, 16 Apr 2020 09:38:24: 5000000 INFO @ Thu, 16 Apr 2020 09:38:29: 9000000 INFO @ Thu, 16 Apr 2020 09:38:30: 13000000 INFO @ Thu, 16 Apr 2020 09:38:32: 6000000 INFO @ Thu, 16 Apr 2020 09:38:38: 10000000 INFO @ Thu, 16 Apr 2020 09:38:39: 14000000 INFO @ Thu, 16 Apr 2020 09:38:41: 7000000 INFO @ Thu, 16 Apr 2020 09:38:47: 11000000 INFO @ Thu, 16 Apr 2020 09:38:48: 15000000 INFO @ Thu, 16 Apr 2020 09:38:49: 8000000 INFO @ Thu, 16 Apr 2020 09:38:55: 12000000 INFO @ Thu, 16 Apr 2020 09:38:56: 16000000 INFO @ Thu, 16 Apr 2020 09:38:57: 9000000 INFO @ Thu, 16 Apr 2020 09:39:04: 13000000 INFO @ Thu, 16 Apr 2020 09:39:05: 17000000 INFO @ Thu, 16 Apr 2020 09:39:06: 10000000 INFO @ Thu, 16 Apr 2020 09:39:13: 14000000 INFO @ Thu, 16 Apr 2020 09:39:13: 18000000 INFO @ Thu, 16 Apr 2020 09:39:14: 11000000 INFO @ Thu, 16 Apr 2020 09:39:21: 15000000 INFO @ Thu, 16 Apr 2020 09:39:22: 19000000 INFO @ Thu, 16 Apr 2020 09:39:23: 12000000 INFO @ Thu, 16 Apr 2020 09:39:30: 16000000 INFO @ Thu, 16 Apr 2020 09:39:31: 20000000 INFO @ Thu, 16 Apr 2020 09:39:31: 13000000 INFO @ Thu, 16 Apr 2020 09:39:38: 17000000 INFO @ Thu, 16 Apr 2020 09:39:39: 21000000 INFO @ Thu, 16 Apr 2020 09:39:39: 14000000 INFO @ Thu, 16 Apr 2020 09:39:47: 18000000 INFO @ Thu, 16 Apr 2020 09:39:48: 15000000 INFO @ Thu, 16 Apr 2020 09:39:48: 22000000 INFO @ Thu, 16 Apr 2020 09:39:56: 19000000 INFO @ Thu, 16 Apr 2020 09:39:56: 16000000 INFO @ Thu, 16 Apr 2020 09:39:57: 23000000 INFO @ Thu, 16 Apr 2020 09:40:04: 20000000 INFO @ Thu, 16 Apr 2020 09:40:04: 17000000 INFO @ Thu, 16 Apr 2020 09:40:05: 24000000 INFO @ Thu, 16 Apr 2020 09:40:13: 18000000 INFO @ Thu, 16 Apr 2020 09:40:13: 21000000 INFO @ Thu, 16 Apr 2020 09:40:14: 25000000 INFO @ Thu, 16 Apr 2020 09:40:21: 19000000 INFO @ Thu, 16 Apr 2020 09:40:21: 22000000 INFO @ Thu, 16 Apr 2020 09:40:23: 26000000 INFO @ Thu, 16 Apr 2020 09:40:29: 20000000 INFO @ Thu, 16 Apr 2020 09:40:30: 23000000 INFO @ Thu, 16 Apr 2020 09:40:31: 27000000 INFO @ Thu, 16 Apr 2020 09:40:38: 21000000 INFO @ Thu, 16 Apr 2020 09:40:39: 24000000 INFO @ Thu, 16 Apr 2020 09:40:40: 28000000 INFO @ Thu, 16 Apr 2020 09:40:46: 22000000 INFO @ Thu, 16 Apr 2020 09:40:48: 25000000 INFO @ Thu, 16 Apr 2020 09:40:49: 29000000 INFO @ Thu, 16 Apr 2020 09:40:54: 23000000 INFO @ Thu, 16 Apr 2020 09:40:56: 26000000 INFO @ Thu, 16 Apr 2020 09:40:58: 30000000 INFO @ Thu, 16 Apr 2020 09:41:03: 24000000 INFO @ Thu, 16 Apr 2020 09:41:05: 27000000 INFO @ Thu, 16 Apr 2020 09:41:07: 31000000 INFO @ Thu, 16 Apr 2020 09:41:11: 25000000 INFO @ Thu, 16 Apr 2020 09:41:14: 28000000 INFO @ Thu, 16 Apr 2020 09:41:15: 32000000 INFO @ Thu, 16 Apr 2020 09:41:20: 26000000 INFO @ Thu, 16 Apr 2020 09:41:22: 29000000 INFO @ Thu, 16 Apr 2020 09:41:24: 33000000 INFO @ Thu, 16 Apr 2020 09:41:28: 27000000 INFO @ Thu, 16 Apr 2020 09:41:31: 30000000 INFO @ Thu, 16 Apr 2020 09:41:33: 34000000 INFO @ Thu, 16 Apr 2020 09:41:37: 28000000 INFO @ Thu, 16 Apr 2020 09:41:40: 31000000 INFO @ Thu, 16 Apr 2020 09:41:40: #1 tag size is determined as 151 bps INFO @ Thu, 16 Apr 2020 09:41:40: #1 tag size = 151 INFO @ Thu, 16 Apr 2020 09:41:40: #1 total tags in treatment: 13220416 INFO @ Thu, 16 Apr 2020 09:41:40: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 09:41:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 09:41:40: #1 tags after filtering in treatment: 10821866 INFO @ Thu, 16 Apr 2020 09:41:40: #1 Redundant rate of treatment: 0.18 INFO @ Thu, 16 Apr 2020 09:41:40: #1 finished! INFO @ Thu, 16 Apr 2020 09:41:40: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 09:41:40: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 09:41:41: #2 number of paired peaks: 2949 INFO @ Thu, 16 Apr 2020 09:41:41: start model_add_line... INFO @ Thu, 16 Apr 2020 09:41:42: start X-correlation... INFO @ Thu, 16 Apr 2020 09:41:42: end of X-cor INFO @ Thu, 16 Apr 2020 09:41:42: #2 finished! INFO @ Thu, 16 Apr 2020 09:41:42: #2 predicted fragment length is 200 bps INFO @ Thu, 16 Apr 2020 09:41:42: #2 alternative fragment length(s) may be 200 bps INFO @ Thu, 16 Apr 2020 09:41:42: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX6386786/SRX6386786.05_model.r WARNING @ Thu, 16 Apr 2020 09:41:42: #2 Since the d (200) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 09:41:42: #2 You may need to consider one of the other alternative d(s): 200 WARNING @ Thu, 16 Apr 2020 09:41:42: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 09:41:42: #3 Call peaks... INFO @ Thu, 16 Apr 2020 09:41:42: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 09:41:45: 29000000 INFO @ Thu, 16 Apr 2020 09:41:48: 32000000 INFO @ Thu, 16 Apr 2020 09:41:54: 30000000 INFO @ Thu, 16 Apr 2020 09:41:57: 33000000 INFO @ Thu, 16 Apr 2020 09:42:02: 31000000 INFO @ Thu, 16 Apr 2020 09:42:05: 34000000 INFO @ Thu, 16 Apr 2020 09:42:08: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 09:42:11: 32000000 INFO @ Thu, 16 Apr 2020 09:42:12: #1 tag size is determined as 151 bps INFO @ Thu, 16 Apr 2020 09:42:12: #1 tag size = 151 INFO @ Thu, 16 Apr 2020 09:42:12: #1 total tags in treatment: 13220416 INFO @ Thu, 16 Apr 2020 09:42:12: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 09:42:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 09:42:12: #1 tags after filtering in treatment: 10821866 INFO @ Thu, 16 Apr 2020 09:42:12: #1 Redundant rate of treatment: 0.18 INFO @ Thu, 16 Apr 2020 09:42:12: #1 finished! INFO @ Thu, 16 Apr 2020 09:42:12: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 09:42:12: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 09:42:13: #2 number of paired peaks: 2949 INFO @ Thu, 16 Apr 2020 09:42:13: start model_add_line... INFO @ Thu, 16 Apr 2020 09:42:13: start X-correlation... INFO @ Thu, 16 Apr 2020 09:42:14: end of X-cor INFO @ Thu, 16 Apr 2020 09:42:14: #2 finished! INFO @ Thu, 16 Apr 2020 09:42:14: #2 predicted fragment length is 200 bps INFO @ Thu, 16 Apr 2020 09:42:14: #2 alternative fragment length(s) may be 200 bps INFO @ Thu, 16 Apr 2020 09:42:14: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX6386786/SRX6386786.10_model.r WARNING @ Thu, 16 Apr 2020 09:42:14: #2 Since the d (200) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 09:42:14: #2 You may need to consider one of the other alternative d(s): 200 WARNING @ Thu, 16 Apr 2020 09:42:14: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 09:42:14: #3 Call peaks... INFO @ Thu, 16 Apr 2020 09:42:14: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 09:42:19: 33000000 INFO @ Thu, 16 Apr 2020 09:42:21: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX6386786/SRX6386786.05_peaks.xls INFO @ Thu, 16 Apr 2020 09:42:21: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX6386786/SRX6386786.05_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 09:42:21: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX6386786/SRX6386786.05_summits.bed INFO @ Thu, 16 Apr 2020 09:42:21: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (15500 records, 4 fields): 15 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 09:42:27: 34000000 INFO @ Thu, 16 Apr 2020 09:42:34: #1 tag size is determined as 151 bps INFO @ Thu, 16 Apr 2020 09:42:34: #1 tag size = 151 INFO @ Thu, 16 Apr 2020 09:42:34: #1 total tags in treatment: 13220416 INFO @ Thu, 16 Apr 2020 09:42:34: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 09:42:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 09:42:34: #1 tags after filtering in treatment: 10821866 INFO @ Thu, 16 Apr 2020 09:42:34: #1 Redundant rate of treatment: 0.18 INFO @ Thu, 16 Apr 2020 09:42:34: #1 finished! INFO @ Thu, 16 Apr 2020 09:42:34: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 09:42:34: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 09:42:35: #2 number of paired peaks: 2949 INFO @ Thu, 16 Apr 2020 09:42:35: start model_add_line... INFO @ Thu, 16 Apr 2020 09:42:35: start X-correlation... INFO @ Thu, 16 Apr 2020 09:42:35: end of X-cor INFO @ Thu, 16 Apr 2020 09:42:35: #2 finished! INFO @ Thu, 16 Apr 2020 09:42:35: #2 predicted fragment length is 200 bps INFO @ Thu, 16 Apr 2020 09:42:35: #2 alternative fragment length(s) may be 200 bps INFO @ Thu, 16 Apr 2020 09:42:35: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX6386786/SRX6386786.20_model.r WARNING @ Thu, 16 Apr 2020 09:42:35: #2 Since the d (200) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 09:42:35: #2 You may need to consider one of the other alternative d(s): 200 WARNING @ Thu, 16 Apr 2020 09:42:35: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 09:42:35: #3 Call peaks... INFO @ Thu, 16 Apr 2020 09:42:35: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 09:42:41: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 09:42:54: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX6386786/SRX6386786.10_peaks.xls INFO @ Thu, 16 Apr 2020 09:42:54: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX6386786/SRX6386786.10_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 09:42:54: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX6386786/SRX6386786.10_summits.bed INFO @ Thu, 16 Apr 2020 09:42:54: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (11481 records, 4 fields): 11 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 09:43:03: #3 Call peaks for each chromosome... BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 16 Apr 2020 09:43:15: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX6386786/SRX6386786.20_peaks.xls INFO @ Thu, 16 Apr 2020 09:43:16: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX6386786/SRX6386786.20_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 09:43:16: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX6386786/SRX6386786.20_summits.bed INFO @ Thu, 16 Apr 2020 09:43:16: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (7209 records, 4 fields): 8 millis CompletedMACS2peakCalling BigWig に変換しました。