Job ID = 6528423 SRX = SRX6386765 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:58:10 prefetch.2.10.7: 1) Downloading 'SRR9624497'... 2020-06-29T14:58:10 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:59:31 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:59:31 prefetch.2.10.7: 'SRR9624497' is valid 2020-06-29T14:59:31 prefetch.2.10.7: 1) 'SRR9624497' was downloaded successfully Read 9943816 spots for SRR9624497/SRR9624497.sra Written 9943816 spots for SRR9624497/SRR9624497.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:27 9943816 reads; of these: 9943816 (100.00%) were unpaired; of these: 427074 (4.29%) aligned 0 times 6569926 (66.07%) aligned exactly 1 time 2946816 (29.63%) aligned >1 times 95.71% overall alignment rate Time searching: 00:03:27 Overall time: 00:03:27 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 950267 / 9516742 = 0.0999 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:08:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6386765/SRX6386765.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6386765/SRX6386765.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6386765/SRX6386765.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6386765/SRX6386765.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:08:42: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:08:42: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:08:48: 1000000 INFO @ Tue, 30 Jun 2020 00:08:54: 2000000 INFO @ Tue, 30 Jun 2020 00:09:00: 3000000 INFO @ Tue, 30 Jun 2020 00:09:06: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:09:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6386765/SRX6386765.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6386765/SRX6386765.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6386765/SRX6386765.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6386765/SRX6386765.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:09:12: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:09:12: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:09:12: 5000000 INFO @ Tue, 30 Jun 2020 00:09:20: 6000000 INFO @ Tue, 30 Jun 2020 00:09:20: 1000000 INFO @ Tue, 30 Jun 2020 00:09:27: 7000000 INFO @ Tue, 30 Jun 2020 00:09:28: 2000000 INFO @ Tue, 30 Jun 2020 00:09:34: 8000000 INFO @ Tue, 30 Jun 2020 00:09:37: 3000000 INFO @ Tue, 30 Jun 2020 00:09:38: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:09:38: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:09:38: #1 total tags in treatment: 8566475 INFO @ Tue, 30 Jun 2020 00:09:38: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:09:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:09:38: #1 tags after filtering in treatment: 8566475 INFO @ Tue, 30 Jun 2020 00:09:38: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:09:38: #1 finished! INFO @ Tue, 30 Jun 2020 00:09:38: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:09:38: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:09:39: #2 number of paired peaks: 92 WARNING @ Tue, 30 Jun 2020 00:09:39: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:09:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6386765/SRX6386765.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6386765/SRX6386765.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6386765/SRX6386765.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6386765/SRX6386765.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:09:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6386765/SRX6386765.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6386765/SRX6386765.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6386765/SRX6386765.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6386765/SRX6386765.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:09:42: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:09:42: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:09:45: 4000000 INFO @ Tue, 30 Jun 2020 00:09:49: 1000000 INFO @ Tue, 30 Jun 2020 00:09:53: 5000000 INFO @ Tue, 30 Jun 2020 00:09:57: 2000000 INFO @ Tue, 30 Jun 2020 00:10:02: 6000000 INFO @ Tue, 30 Jun 2020 00:10:04: 3000000 INFO @ Tue, 30 Jun 2020 00:10:10: 7000000 INFO @ Tue, 30 Jun 2020 00:10:12: 4000000 INFO @ Tue, 30 Jun 2020 00:10:18: 8000000 INFO @ Tue, 30 Jun 2020 00:10:19: 5000000 INFO @ Tue, 30 Jun 2020 00:10:23: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:10:23: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:10:23: #1 total tags in treatment: 8566475 INFO @ Tue, 30 Jun 2020 00:10:23: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:10:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:10:23: #1 tags after filtering in treatment: 8566475 INFO @ Tue, 30 Jun 2020 00:10:23: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:10:23: #1 finished! INFO @ Tue, 30 Jun 2020 00:10:23: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:10:23: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:10:23: #2 number of paired peaks: 92 WARNING @ Tue, 30 Jun 2020 00:10:23: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:10:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6386765/SRX6386765.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6386765/SRX6386765.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6386765/SRX6386765.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6386765/SRX6386765.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:10:26: 6000000 INFO @ Tue, 30 Jun 2020 00:10:33: 7000000 INFO @ Tue, 30 Jun 2020 00:10:40: 8000000 INFO @ Tue, 30 Jun 2020 00:10:43: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:10:43: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:10:43: #1 total tags in treatment: 8566475 INFO @ Tue, 30 Jun 2020 00:10:43: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:10:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:10:43: #1 tags after filtering in treatment: 8566475 INFO @ Tue, 30 Jun 2020 00:10:43: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:10:43: #1 finished! INFO @ Tue, 30 Jun 2020 00:10:43: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:10:43: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:10:44: #2 number of paired peaks: 92 WARNING @ Tue, 30 Jun 2020 00:10:44: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:10:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6386765/SRX6386765.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6386765/SRX6386765.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6386765/SRX6386765.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6386765/SRX6386765.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。