Job ID = 5720928 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-04-15T18:18:07 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T18:18:07 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T18:18:07 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 10,534,288 reads read : 10,534,288 reads written : 10,534,288 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:21 10534288 reads; of these: 10534288 (100.00%) were unpaired; of these: 4439712 (42.15%) aligned 0 times 4276856 (40.60%) aligned exactly 1 time 1817720 (17.26%) aligned >1 times 57.85% overall alignment rate Time searching: 00:02:21 Overall time: 00:02:21 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 1576223 / 6094576 = 0.2586 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 03:26:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6386729/SRX6386729.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6386729/SRX6386729.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6386729/SRX6386729.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6386729/SRX6386729.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 03:26:03: #1 read tag files... INFO @ Thu, 16 Apr 2020 03:26:03: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 03:26:08: 1000000 INFO @ Thu, 16 Apr 2020 03:26:13: 2000000 INFO @ Thu, 16 Apr 2020 03:26:18: 3000000 INFO @ Thu, 16 Apr 2020 03:26:23: 4000000 INFO @ Thu, 16 Apr 2020 03:26:25: #1 tag size is determined as 51 bps INFO @ Thu, 16 Apr 2020 03:26:25: #1 tag size = 51 INFO @ Thu, 16 Apr 2020 03:26:25: #1 total tags in treatment: 4518353 INFO @ Thu, 16 Apr 2020 03:26:25: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 03:26:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 03:26:25: #1 tags after filtering in treatment: 4518353 INFO @ Thu, 16 Apr 2020 03:26:25: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 16 Apr 2020 03:26:25: #1 finished! INFO @ Thu, 16 Apr 2020 03:26:25: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 03:26:25: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 03:26:26: #2 number of paired peaks: 211 WARNING @ Thu, 16 Apr 2020 03:26:26: Fewer paired peaks (211) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 211 pairs to build model! INFO @ Thu, 16 Apr 2020 03:26:26: start model_add_line... INFO @ Thu, 16 Apr 2020 03:26:26: start X-correlation... INFO @ Thu, 16 Apr 2020 03:26:26: end of X-cor INFO @ Thu, 16 Apr 2020 03:26:26: #2 finished! INFO @ Thu, 16 Apr 2020 03:26:26: #2 predicted fragment length is 59 bps INFO @ Thu, 16 Apr 2020 03:26:26: #2 alternative fragment length(s) may be 59 bps INFO @ Thu, 16 Apr 2020 03:26:26: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX6386729/SRX6386729.05_model.r WARNING @ Thu, 16 Apr 2020 03:26:26: #2 Since the d (59) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 03:26:26: #2 You may need to consider one of the other alternative d(s): 59 WARNING @ Thu, 16 Apr 2020 03:26:26: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 03:26:26: #3 Call peaks... INFO @ Thu, 16 Apr 2020 03:26:26: #3 Pre-compute pvalue-qvalue table... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 03:26:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6386729/SRX6386729.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6386729/SRX6386729.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6386729/SRX6386729.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6386729/SRX6386729.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 03:26:33: #1 read tag files... INFO @ Thu, 16 Apr 2020 03:26:33: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 03:26:35: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 03:26:39: 1000000 INFO @ Thu, 16 Apr 2020 03:26:40: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX6386729/SRX6386729.05_peaks.xls INFO @ Thu, 16 Apr 2020 03:26:40: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX6386729/SRX6386729.05_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 03:26:40: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX6386729/SRX6386729.05_summits.bed INFO @ Thu, 16 Apr 2020 03:26:40: Done! pass1 - making usageList (14 chroms): 0 millis pass2 - checking and writing primary data (969 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 03:26:44: 2000000 INFO @ Thu, 16 Apr 2020 03:26:50: 3000000 INFO @ Thu, 16 Apr 2020 03:26:56: 4000000 INFO @ Thu, 16 Apr 2020 03:26:58: #1 tag size is determined as 51 bps INFO @ Thu, 16 Apr 2020 03:26:58: #1 tag size = 51 INFO @ Thu, 16 Apr 2020 03:26:58: #1 total tags in treatment: 4518353 INFO @ Thu, 16 Apr 2020 03:26:58: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 03:26:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 03:26:59: #1 tags after filtering in treatment: 4518353 INFO @ Thu, 16 Apr 2020 03:26:59: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 16 Apr 2020 03:26:59: #1 finished! INFO @ Thu, 16 Apr 2020 03:26:59: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 03:26:59: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 03:26:59: #2 number of paired peaks: 211 WARNING @ Thu, 16 Apr 2020 03:26:59: Fewer paired peaks (211) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 211 pairs to build model! INFO @ Thu, 16 Apr 2020 03:26:59: start model_add_line... INFO @ Thu, 16 Apr 2020 03:26:59: start X-correlation... INFO @ Thu, 16 Apr 2020 03:26:59: end of X-cor INFO @ Thu, 16 Apr 2020 03:26:59: #2 finished! INFO @ Thu, 16 Apr 2020 03:26:59: #2 predicted fragment length is 59 bps INFO @ Thu, 16 Apr 2020 03:26:59: #2 alternative fragment length(s) may be 59 bps INFO @ Thu, 16 Apr 2020 03:26:59: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX6386729/SRX6386729.10_model.r WARNING @ Thu, 16 Apr 2020 03:26:59: #2 Since the d (59) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 03:26:59: #2 You may need to consider one of the other alternative d(s): 59 WARNING @ Thu, 16 Apr 2020 03:26:59: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 03:26:59: #3 Call peaks... INFO @ Thu, 16 Apr 2020 03:26:59: #3 Pre-compute pvalue-qvalue table... BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 03:27:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6386729/SRX6386729.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6386729/SRX6386729.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6386729/SRX6386729.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6386729/SRX6386729.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 03:27:03: #1 read tag files... INFO @ Thu, 16 Apr 2020 03:27:03: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 03:27:08: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 03:27:09: 1000000 INFO @ Thu, 16 Apr 2020 03:27:13: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX6386729/SRX6386729.10_peaks.xls INFO @ Thu, 16 Apr 2020 03:27:13: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX6386729/SRX6386729.10_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 03:27:13: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX6386729/SRX6386729.10_summits.bed INFO @ Thu, 16 Apr 2020 03:27:13: Done! pass1 - making usageList (10 chroms): 0 millis pass2 - checking and writing primary data (567 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 03:27:14: 2000000 INFO @ Thu, 16 Apr 2020 03:27:20: 3000000 INFO @ Thu, 16 Apr 2020 03:27:26: 4000000 INFO @ Thu, 16 Apr 2020 03:27:29: #1 tag size is determined as 51 bps INFO @ Thu, 16 Apr 2020 03:27:29: #1 tag size = 51 INFO @ Thu, 16 Apr 2020 03:27:29: #1 total tags in treatment: 4518353 INFO @ Thu, 16 Apr 2020 03:27:29: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 03:27:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 03:27:29: #1 tags after filtering in treatment: 4518353 INFO @ Thu, 16 Apr 2020 03:27:29: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 16 Apr 2020 03:27:29: #1 finished! INFO @ Thu, 16 Apr 2020 03:27:29: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 03:27:29: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 03:27:29: #2 number of paired peaks: 211 WARNING @ Thu, 16 Apr 2020 03:27:29: Fewer paired peaks (211) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 211 pairs to build model! INFO @ Thu, 16 Apr 2020 03:27:29: start model_add_line... INFO @ Thu, 16 Apr 2020 03:27:29: start X-correlation... INFO @ Thu, 16 Apr 2020 03:27:29: end of X-cor INFO @ Thu, 16 Apr 2020 03:27:29: #2 finished! INFO @ Thu, 16 Apr 2020 03:27:29: #2 predicted fragment length is 59 bps INFO @ Thu, 16 Apr 2020 03:27:29: #2 alternative fragment length(s) may be 59 bps INFO @ Thu, 16 Apr 2020 03:27:29: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX6386729/SRX6386729.20_model.r WARNING @ Thu, 16 Apr 2020 03:27:29: #2 Since the d (59) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 03:27:29: #2 You may need to consider one of the other alternative d(s): 59 WARNING @ Thu, 16 Apr 2020 03:27:29: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 03:27:29: #3 Call peaks... INFO @ Thu, 16 Apr 2020 03:27:29: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 03:27:39: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 03:27:43: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX6386729/SRX6386729.20_peaks.xls INFO @ Thu, 16 Apr 2020 03:27:43: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX6386729/SRX6386729.20_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 03:27:43: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX6386729/SRX6386729.20_summits.bed INFO @ Thu, 16 Apr 2020 03:27:43: Done! pass1 - making usageList (9 chroms): 1 millis pass2 - checking and writing primary data (272 records, 4 fields): 1 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。