Job ID = 4178579 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 58,591,094 reads read : 117,182,188 reads written : 58,591,094 reads 0-length : 58,591,094 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:22:08 58591094 reads; of these: 58591094 (100.00%) were unpaired; of these: 4741450 (8.09%) aligned 0 times 39954793 (68.19%) aligned exactly 1 time 13894851 (23.71%) aligned >1 times 91.91% overall alignment rate Time searching: 00:22:08 Overall time: 00:22:08 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 19765862 / 53849644 = 0.3671 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Thu, 05 Dec 2019 14:07:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5827843/SRX5827843.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5827843/SRX5827843.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5827843/SRX5827843.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5827843/SRX5827843.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 05 Dec 2019 14:07:54: #1 read tag files... INFO @ Thu, 05 Dec 2019 14:07:54: #1 read treatment tags... INFO @ Thu, 05 Dec 2019 14:08:04: 1000000 INFO @ Thu, 05 Dec 2019 14:08:14: 2000000 INFO @ Thu, 05 Dec 2019 14:08:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5827843/SRX5827843.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5827843/SRX5827843.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5827843/SRX5827843.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5827843/SRX5827843.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 05 Dec 2019 14:08:24: #1 read tag files... INFO @ Thu, 05 Dec 2019 14:08:24: #1 read treatment tags... INFO @ Thu, 05 Dec 2019 14:08:27: 3000000 INFO @ Thu, 05 Dec 2019 14:08:35: 1000000 INFO @ Thu, 05 Dec 2019 14:08:37: 4000000 INFO @ Thu, 05 Dec 2019 14:08:46: 2000000 INFO @ Thu, 05 Dec 2019 14:08:48: 5000000 BedGraph に変換中... INFO @ Thu, 05 Dec 2019 14:08:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5827843/SRX5827843.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5827843/SRX5827843.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5827843/SRX5827843.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5827843/SRX5827843.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 05 Dec 2019 14:08:54: #1 read tag files... INFO @ Thu, 05 Dec 2019 14:08:54: #1 read treatment tags... INFO @ Thu, 05 Dec 2019 14:08:57: 3000000 INFO @ Thu, 05 Dec 2019 14:08:59: 6000000 INFO @ Thu, 05 Dec 2019 14:09:06: 1000000 INFO @ Thu, 05 Dec 2019 14:09:08: 4000000 INFO @ Thu, 05 Dec 2019 14:09:11: 7000000 INFO @ Thu, 05 Dec 2019 14:09:19: 2000000 INFO @ Thu, 05 Dec 2019 14:09:20: 5000000 INFO @ Thu, 05 Dec 2019 14:09:24: 8000000 INFO @ Thu, 05 Dec 2019 14:09:32: 3000000 INFO @ Thu, 05 Dec 2019 14:09:35: 6000000 INFO @ Thu, 05 Dec 2019 14:09:37: 9000000 INFO @ Thu, 05 Dec 2019 14:09:44: 4000000 INFO @ Thu, 05 Dec 2019 14:09:47: 7000000 INFO @ Thu, 05 Dec 2019 14:09:49: 10000000 INFO @ Thu, 05 Dec 2019 14:09:56: 5000000 INFO @ Thu, 05 Dec 2019 14:10:00: 8000000 INFO @ Thu, 05 Dec 2019 14:10:00: 11000000 INFO @ Thu, 05 Dec 2019 14:10:08: 6000000 INFO @ Thu, 05 Dec 2019 14:10:12: 9000000 INFO @ Thu, 05 Dec 2019 14:10:12: 12000000 INFO @ Thu, 05 Dec 2019 14:10:20: 7000000 INFO @ Thu, 05 Dec 2019 14:10:23: 10000000 INFO @ Thu, 05 Dec 2019 14:10:24: 13000000 INFO @ Thu, 05 Dec 2019 14:10:32: 8000000 INFO @ Thu, 05 Dec 2019 14:10:34: 11000000 INFO @ Thu, 05 Dec 2019 14:10:35: 14000000 INFO @ Thu, 05 Dec 2019 14:10:44: 9000000 INFO @ Thu, 05 Dec 2019 14:10:45: 12000000 INFO @ Thu, 05 Dec 2019 14:10:47: 15000000 INFO @ Thu, 05 Dec 2019 14:10:55: 10000000 INFO @ Thu, 05 Dec 2019 14:10:55: 13000000 INFO @ Thu, 05 Dec 2019 14:10:58: 16000000 INFO @ Thu, 05 Dec 2019 14:11:05: 14000000 INFO @ Thu, 05 Dec 2019 14:11:06: 11000000 INFO @ Thu, 05 Dec 2019 14:11:09: 17000000 INFO @ Thu, 05 Dec 2019 14:11:15: 15000000 INFO @ Thu, 05 Dec 2019 14:11:18: 12000000 INFO @ Thu, 05 Dec 2019 14:11:21: 18000000 INFO @ Thu, 05 Dec 2019 14:11:25: 16000000 INFO @ Thu, 05 Dec 2019 14:11:30: 13000000 INFO @ Thu, 05 Dec 2019 14:11:33: 19000000 INFO @ Thu, 05 Dec 2019 14:11:34: 17000000 INFO @ Thu, 05 Dec 2019 14:11:44: 14000000 INFO @ Thu, 05 Dec 2019 14:11:45: 18000000 INFO @ Thu, 05 Dec 2019 14:11:48: 20000000 INFO @ Thu, 05 Dec 2019 14:11:55: 19000000 INFO @ Thu, 05 Dec 2019 14:11:57: 15000000 INFO @ Thu, 05 Dec 2019 14:12:00: 21000000 INFO @ Thu, 05 Dec 2019 14:12:06: 20000000 INFO @ Thu, 05 Dec 2019 14:12:07: 16000000 INFO @ Thu, 05 Dec 2019 14:12:12: 22000000 INFO @ Thu, 05 Dec 2019 14:12:16: 21000000 INFO @ Thu, 05 Dec 2019 14:12:19: 17000000 INFO @ Thu, 05 Dec 2019 14:12:26: 23000000 INFO @ Thu, 05 Dec 2019 14:12:27: 22000000 INFO @ Thu, 05 Dec 2019 14:12:32: 18000000 INFO @ Thu, 05 Dec 2019 14:12:37: 23000000 INFO @ Thu, 05 Dec 2019 14:12:37: 24000000 INFO @ Thu, 05 Dec 2019 14:12:43: 19000000 INFO @ Thu, 05 Dec 2019 14:12:47: 24000000 INFO @ Thu, 05 Dec 2019 14:12:49: 25000000 INFO @ Thu, 05 Dec 2019 14:12:55: 20000000 INFO @ Thu, 05 Dec 2019 14:12:57: 25000000 INFO @ Thu, 05 Dec 2019 14:13:01: 26000000 INFO @ Thu, 05 Dec 2019 14:13:06: 21000000 INFO @ Thu, 05 Dec 2019 14:13:07: 26000000 INFO @ Thu, 05 Dec 2019 14:13:13: 27000000 INFO @ Thu, 05 Dec 2019 14:13:17: 27000000 INFO @ Thu, 05 Dec 2019 14:13:18: 22000000 INFO @ Thu, 05 Dec 2019 14:13:25: 28000000 INFO @ Thu, 05 Dec 2019 14:13:27: 28000000 INFO @ Thu, 05 Dec 2019 14:13:30: 23000000 INFO @ Thu, 05 Dec 2019 14:13:37: 29000000 INFO @ Thu, 05 Dec 2019 14:13:37: 29000000 INFO @ Thu, 05 Dec 2019 14:13:42: 24000000 INFO @ Thu, 05 Dec 2019 14:13:48: 30000000 INFO @ Thu, 05 Dec 2019 14:13:50: 30000000 INFO @ Thu, 05 Dec 2019 14:13:54: 25000000 INFO @ Thu, 05 Dec 2019 14:13:59: 31000000 INFO @ Thu, 05 Dec 2019 14:14:02: 31000000 INFO @ Thu, 05 Dec 2019 14:14:05: 26000000 INFO @ Thu, 05 Dec 2019 14:14:09: 32000000 INFO @ Thu, 05 Dec 2019 14:14:13: 32000000 INFO @ Thu, 05 Dec 2019 14:14:18: 27000000 INFO @ Thu, 05 Dec 2019 14:14:20: 33000000 INFO @ Thu, 05 Dec 2019 14:14:25: 33000000 INFO @ Thu, 05 Dec 2019 14:14:30: 28000000 INFO @ Thu, 05 Dec 2019 14:14:30: 34000000 INFO @ Thu, 05 Dec 2019 14:14:31: #1 tag size is determined as 51 bps INFO @ Thu, 05 Dec 2019 14:14:31: #1 tag size = 51 INFO @ Thu, 05 Dec 2019 14:14:31: #1 total tags in treatment: 34083782 INFO @ Thu, 05 Dec 2019 14:14:31: #1 user defined the maximum tags... INFO @ Thu, 05 Dec 2019 14:14:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 05 Dec 2019 14:14:32: #1 tags after filtering in treatment: 34083782 INFO @ Thu, 05 Dec 2019 14:14:32: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 05 Dec 2019 14:14:32: #1 finished! INFO @ Thu, 05 Dec 2019 14:14:32: #2 Build Peak Model... INFO @ Thu, 05 Dec 2019 14:14:32: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 05 Dec 2019 14:14:34: #2 number of paired peaks: 52 WARNING @ Thu, 05 Dec 2019 14:14:34: Too few paired peaks (52) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 05 Dec 2019 14:14:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5827843/SRX5827843.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5827843/SRX5827843.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5827843/SRX5827843.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5827843/SRX5827843.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 05 Dec 2019 14:14:37: 34000000 INFO @ Thu, 05 Dec 2019 14:14:38: #1 tag size is determined as 51 bps INFO @ Thu, 05 Dec 2019 14:14:38: #1 tag size = 51 INFO @ Thu, 05 Dec 2019 14:14:38: #1 total tags in treatment: 34083782 INFO @ Thu, 05 Dec 2019 14:14:38: #1 user defined the maximum tags... INFO @ Thu, 05 Dec 2019 14:14:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 05 Dec 2019 14:14:39: #1 tags after filtering in treatment: 34083782 INFO @ Thu, 05 Dec 2019 14:14:39: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 05 Dec 2019 14:14:39: #1 finished! INFO @ Thu, 05 Dec 2019 14:14:39: #2 Build Peak Model... INFO @ Thu, 05 Dec 2019 14:14:39: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 05 Dec 2019 14:14:41: 29000000 INFO @ Thu, 05 Dec 2019 14:14:42: #2 number of paired peaks: 52 WARNING @ Thu, 05 Dec 2019 14:14:42: Too few paired peaks (52) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 05 Dec 2019 14:14:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5827843/SRX5827843.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5827843/SRX5827843.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5827843/SRX5827843.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5827843/SRX5827843.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 05 Dec 2019 14:14:55: 30000000 INFO @ Thu, 05 Dec 2019 14:15:05: 31000000 INFO @ Thu, 05 Dec 2019 14:15:15: 32000000 INFO @ Thu, 05 Dec 2019 14:15:25: 33000000 INFO @ Thu, 05 Dec 2019 14:15:36: 34000000 INFO @ Thu, 05 Dec 2019 14:15:37: #1 tag size is determined as 51 bps INFO @ Thu, 05 Dec 2019 14:15:37: #1 tag size = 51 INFO @ Thu, 05 Dec 2019 14:15:37: #1 total tags in treatment: 34083782 INFO @ Thu, 05 Dec 2019 14:15:37: #1 user defined the maximum tags... INFO @ Thu, 05 Dec 2019 14:15:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 05 Dec 2019 14:15:38: #1 tags after filtering in treatment: 34083782 INFO @ Thu, 05 Dec 2019 14:15:38: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 05 Dec 2019 14:15:38: #1 finished! INFO @ Thu, 05 Dec 2019 14:15:38: #2 Build Peak Model... INFO @ Thu, 05 Dec 2019 14:15:38: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 05 Dec 2019 14:15:41: #2 number of paired peaks: 52 WARNING @ Thu, 05 Dec 2019 14:15:41: Too few paired peaks (52) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 05 Dec 2019 14:15:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5827843/SRX5827843.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5827843/SRX5827843.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5827843/SRX5827843.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5827843/SRX5827843.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。