Job ID = 6528407 SRX = SRX5775938 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T15:28:02 prefetch.2.10.7: 1) Downloading 'SRR8997141'... 2020-06-29T15:28:02 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:33:10 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:33:10 prefetch.2.10.7: 1) 'SRR8997141' was downloaded successfully Read 30566777 spots for SRR8997141/SRR8997141.sra Written 30566777 spots for SRR8997141/SRR8997141.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:06 30566777 reads; of these: 30566777 (100.00%) were unpaired; of these: 776339 (2.54%) aligned 0 times 20439276 (66.87%) aligned exactly 1 time 9351162 (30.59%) aligned >1 times 97.46% overall alignment rate Time searching: 00:15:06 Overall time: 00:15:06 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 10301788 / 29790438 = 0.3458 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:11:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5775938/SRX5775938.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5775938/SRX5775938.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5775938/SRX5775938.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5775938/SRX5775938.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:11:40: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:11:40: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:11:48: 1000000 INFO @ Tue, 30 Jun 2020 01:11:56: 2000000 INFO @ Tue, 30 Jun 2020 01:12:04: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:12:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5775938/SRX5775938.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5775938/SRX5775938.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5775938/SRX5775938.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5775938/SRX5775938.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:12:09: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:12:09: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:12:12: 4000000 INFO @ Tue, 30 Jun 2020 01:12:17: 1000000 INFO @ Tue, 30 Jun 2020 01:12:20: 5000000 INFO @ Tue, 30 Jun 2020 01:12:26: 2000000 INFO @ Tue, 30 Jun 2020 01:12:29: 6000000 INFO @ Tue, 30 Jun 2020 01:12:35: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:12:37: 7000000 INFO @ Tue, 30 Jun 2020 01:12:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5775938/SRX5775938.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5775938/SRX5775938.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5775938/SRX5775938.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5775938/SRX5775938.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:12:39: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:12:39: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:12:43: 4000000 INFO @ Tue, 30 Jun 2020 01:12:46: 8000000 INFO @ Tue, 30 Jun 2020 01:12:48: 1000000 INFO @ Tue, 30 Jun 2020 01:12:52: 5000000 INFO @ Tue, 30 Jun 2020 01:12:55: 9000000 INFO @ Tue, 30 Jun 2020 01:12:57: 2000000 INFO @ Tue, 30 Jun 2020 01:13:01: 6000000 INFO @ Tue, 30 Jun 2020 01:13:04: 10000000 INFO @ Tue, 30 Jun 2020 01:13:06: 3000000 INFO @ Tue, 30 Jun 2020 01:13:10: 7000000 INFO @ Tue, 30 Jun 2020 01:13:12: 11000000 INFO @ Tue, 30 Jun 2020 01:13:15: 4000000 INFO @ Tue, 30 Jun 2020 01:13:18: 8000000 INFO @ Tue, 30 Jun 2020 01:13:21: 12000000 INFO @ Tue, 30 Jun 2020 01:13:24: 5000000 INFO @ Tue, 30 Jun 2020 01:13:27: 9000000 INFO @ Tue, 30 Jun 2020 01:13:30: 13000000 INFO @ Tue, 30 Jun 2020 01:13:33: 6000000 INFO @ Tue, 30 Jun 2020 01:13:35: 10000000 INFO @ Tue, 30 Jun 2020 01:13:39: 14000000 INFO @ Tue, 30 Jun 2020 01:13:42: 7000000 INFO @ Tue, 30 Jun 2020 01:13:44: 11000000 INFO @ Tue, 30 Jun 2020 01:13:47: 15000000 INFO @ Tue, 30 Jun 2020 01:13:50: 8000000 INFO @ Tue, 30 Jun 2020 01:13:53: 12000000 INFO @ Tue, 30 Jun 2020 01:13:56: 16000000 INFO @ Tue, 30 Jun 2020 01:13:59: 9000000 INFO @ Tue, 30 Jun 2020 01:14:01: 13000000 INFO @ Tue, 30 Jun 2020 01:14:05: 17000000 INFO @ Tue, 30 Jun 2020 01:14:08: 10000000 INFO @ Tue, 30 Jun 2020 01:14:10: 14000000 INFO @ Tue, 30 Jun 2020 01:14:13: 18000000 INFO @ Tue, 30 Jun 2020 01:14:17: 11000000 INFO @ Tue, 30 Jun 2020 01:14:19: 15000000 INFO @ Tue, 30 Jun 2020 01:14:22: 19000000 INFO @ Tue, 30 Jun 2020 01:14:26: 12000000 INFO @ Tue, 30 Jun 2020 01:14:26: #1 tag size is determined as 75 bps INFO @ Tue, 30 Jun 2020 01:14:26: #1 tag size = 75 INFO @ Tue, 30 Jun 2020 01:14:26: #1 total tags in treatment: 19488650 INFO @ Tue, 30 Jun 2020 01:14:26: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:14:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:14:27: #1 tags after filtering in treatment: 19488650 INFO @ Tue, 30 Jun 2020 01:14:27: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:14:27: #1 finished! INFO @ Tue, 30 Jun 2020 01:14:27: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:14:27: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:14:27: 16000000 INFO @ Tue, 30 Jun 2020 01:14:28: #2 number of paired peaks: 73 WARNING @ Tue, 30 Jun 2020 01:14:28: Too few paired peaks (73) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:14:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5775938/SRX5775938.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5775938/SRX5775938.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5775938/SRX5775938.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5775938/SRX5775938.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 01:14:34: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 01:14:36: 17000000 INFO @ Tue, 30 Jun 2020 01:14:43: 14000000 INFO @ Tue, 30 Jun 2020 01:14:44: 18000000 INFO @ Tue, 30 Jun 2020 01:14:51: 15000000 INFO @ Tue, 30 Jun 2020 01:14:52: 19000000 INFO @ Tue, 30 Jun 2020 01:14:56: #1 tag size is determined as 75 bps INFO @ Tue, 30 Jun 2020 01:14:56: #1 tag size = 75 INFO @ Tue, 30 Jun 2020 01:14:56: #1 total tags in treatment: 19488650 INFO @ Tue, 30 Jun 2020 01:14:56: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:14:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:14:57: #1 tags after filtering in treatment: 19488650 INFO @ Tue, 30 Jun 2020 01:14:57: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:14:57: #1 finished! INFO @ Tue, 30 Jun 2020 01:14:57: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:14:57: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:14:58: #2 number of paired peaks: 73 WARNING @ Tue, 30 Jun 2020 01:14:58: Too few paired peaks (73) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:14:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5775938/SRX5775938.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5775938/SRX5775938.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5775938/SRX5775938.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5775938/SRX5775938.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 01:14:59: 16000000 INFO @ Tue, 30 Jun 2020 01:15:06: 17000000 INFO @ Tue, 30 Jun 2020 01:15:14: 18000000 INFO @ Tue, 30 Jun 2020 01:15:21: 19000000 INFO @ Tue, 30 Jun 2020 01:15:25: #1 tag size is determined as 75 bps INFO @ Tue, 30 Jun 2020 01:15:25: #1 tag size = 75 INFO @ Tue, 30 Jun 2020 01:15:25: #1 total tags in treatment: 19488650 INFO @ Tue, 30 Jun 2020 01:15:25: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:15:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:15:25: #1 tags after filtering in treatment: 19488650 INFO @ Tue, 30 Jun 2020 01:15:25: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:15:25: #1 finished! INFO @ Tue, 30 Jun 2020 01:15:25: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:15:25: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:15:26: #2 number of paired peaks: 73 WARNING @ Tue, 30 Jun 2020 01:15:26: Too few paired peaks (73) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:15:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5775938/SRX5775938.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5775938/SRX5775938.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5775938/SRX5775938.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5775938/SRX5775938.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。